Histomorphometrical study of silver carp fish testis in two age classes

In this research, morphological and histomorphometrical structure of testis of 20 silver male carp fish were studied in two classes or groups. Group1 was composed of 10 fish with average (±SD) weight of 1.247+0.656kg and average(±SD) length of 43.675+1.414cm with about 2 years age, Group2 was composed of 10 fish with average(±SD) weight of 5.716+0.519kg and average(±SD) length of 81.5+1.643cm. Average (±SD) weight of testis were 2.34+1.47gr and 83.33+25.81gr with average (±SD) GSI of 0.187+0.224 and 1.457+4.974 in groups 1 and 2 respectively. Samples from testis were taken by maximum thickness of 0.5cm and after fixation in bouin , s fixative and 5-6µm thickness section were made routine paraffin embedding method and stained by Hematoxylin-Eosin and PAS staining. The microscopic results showed that the silver carp testis was lobular and cystic type in two groups. In group 1, there was no spermatozoon activity and PGCs were only germ cells in the cysts. But in group2, the numbers of PGCs were decreased significantly and spermatogenic cells were seen in different phases including spermatogonia, primary and secondary spermatocysts, early and late spermatid, and spermatozoa which each one was located in a separated cyst. There was no significant difference in nucleus diameter of PGCs in testis of group1 (6.97+0.438µ) and group (6.13+0.438µ). In group2, the nucleolus diameter of spermatogonia was 2.97+0.112µm, primary spermatocyt 3.59+0.107µ, early spermatid 1.59+0.761µ, late spermatid 1.24+0.132µ, spermatozoa 1.16+0.054µ, and the length of spermatozoia 17.412+1.946µ. The interesting finding was immature testis in fish of group 1 with average weigh (1.247+0.656kg) and average length (43.675+1.414cm) in about 2 years age and mature testis in fish of group 2 with average weight of (5.716+0.519kg) and average length of (81.5+1.643cm) with about 4 years age in Khuzestan climate conditions

Influence des paramètres de composition sur le comportement du béton autoplaçant à l’état frais

La présente étude s’inscrit dans le cadre des travaux de recherche menés au département de génie civil de l'université de Mostaganem, en Algérie. Elle concerne l’étude de l’influence des paramètres de composition sur le comportement du béton autoplaçant à l'état frais. Elle a pour objectif de comprendre les rôles joués par les différents constituants susceptibles d’entrer dans la formulation d'un BAP à base de matériaux locaux notamment le sable de mer et le sable de carrière (considéré comme déchet naturel) et de mettre en évidence les éventuelles interactions entre constituants. Les résultats obtenus offrent de belles perspectives pour optimiser les BAP. Nos essais ont permis de développer une variété de formulations de béton autoplaçant répondant aux critères rhéologiques (bonne déformabilité, moins de ressuage, absence de ségrégation).Mots-clés : Béton autoplaçant, superplastifiant, fines calcaires, matériaux locaux, béton frais

Isolation of common carp ovarian follicular cells and evaluation of their endocrine activity in primary cell culture

To study viability and activity of isolated common carp, Cyprinus carpio, ovarian follicular cells (granulosa and theca cells), 17-α-Hydroxy progesterone (17α-OHP) and 17β-Estradiol (E2) levels were estimated in the culture media of cultivated carp ovarian follicular cells, using radioimmunoassay (RIA). Oocytes were isolated from the ovaries of female carp. Interstitial tissue was manually removed in order to obtain single oocytes surrounded only by the follicular envelope. Such a preparation was trypsinized at room temperature. Follicular cell suspension contained both cells and cell clumps. It was the mixture of theca (T) and granulosa (G) cells. The cells were grown as monolayer in 24-well microplates in M199 medium supplemented with FBS. Culture media were analyzed for estrogen and progesterone content by appropriate radioimmunoassay. Trypsinization of ovarian follicles resulted in the formation of the cell suspension which contained a mixture of G and T cells. The follicular cells attached to glass and grew well during culture period. E2 was the main steroid hormone secreted by cultivated cells. Estrogen secretion increased by 415.52 ± 25 pg/ml at the first 3 days up to 530.25 ± 55.8 pg/ml on day 5 and it didn't change significantly until the end of the experiment. 17α-OHP secretion, however, gradually increased from 23.84 ± 8.2 pg/ml at the beginning of culture up to 35.76 ± 5.4 pg/ml at the end of cultivating. As the result of the present study the fish follicular cells grown in tissue culture were steroidogenically active cells as expressed by the secretion of E2 and P4 and the E2 was a dominant hormone secreted by isolated follicular cells, which it correlated closely with vitellogenes stage

Primary Malignant Cardiac Tumors (PMCTs) Successful Resection of a Huge Liposarcoma of the Heart

Primary cardiac tumors are rare with an incidence ranging from 0.001% to 0.03% in autopsy series. The prognosis of cardiac sarcomas remains poor because it proliferates rapidly, and distant metastases are often found at diagnosis. We present a case of liposarcoma in the atrium of the heart as case report and same time ,we do a literature review about it.  Case Report: We present the case of a young 30 years old female with a significant tumor of cardiac liposarcoma, with chief complaint of dyspnea, tachycardia and heart failure even angina pectoris. Conclusion: The diagnostician’s differential diagnosis must be broad when encountering common chief complaints, such as tachycardia, heart failure and angina pectoris

An in situ exploration of subsurface defect migration to a liquid water‐exposed rutile TiO2(110) surface by XPS

The ability of titanium dioixide to split water into OH− and H+ species is heavily dependent on the behaviour of defects in the crystal structure at or near the surface. We present an in situ study of defect migration in rutile TiO2(110) conducted using X‐ray photoelectron spectroscopy (XPS). First, surface and subsurface defects were created in the crystal by argon ion sputtering. Subsequent in situ exposure of the defective crystal to liquid water healed the surface defects, whereas the subsurface remained defective. The sample was then annealed while XPS was used to monitor the concentration of titanium defects. At low annealing temperatures, Ti3+ was observed to migrate from the subsurface to the surface. Further annealing gradually restored the surface and subsurface to the defect‐free Ti4+ form, during which the changes in abundance of Ti1+, Ti2+ and Ti3+ defects are discussed

Traumatic Pulmonary Pneumatoceles (Pseudocyst)

Introduction: Traumatic pulmonary parenchymal cavitary lesions (TPPCs) are pulmonary pseudocysts raiding secondary to lung contusion. Method: To provide an overview of the etiology, presentation, diagnosis and treatment of TPPCs, and to discuss this in the context of a cohort of 12 retrospectively reviewed patients with TPPCs presenting to Sahlgrenska University Hospital, Gothenburg, Sweden, from January 2014 to December 2016. Between January 2014 and December 2016, a total of twelve trauma patients presented to Sahlgrenska University Hospital with TPPC following blunt trauma. Results: TPPCs are of limited clinical consequence. Inexperienced clinicians may treat these inappropriately. A Computed Tomography (CT) scan is the investigation of choice. Treatment is symptomatic. Intervention is indicated only in case of complications

Function of specialized regulatory proteins and signaling pathways in exercise-induced muscle mitochondrial biogenesis

AbstractSkeletal muscle mitochondrial content and function are regulated by a number of specialized molecular pathways that remain to be fully defined. Although a number of proteins have been identified to be important for the maintenance of mitochondria in quiescent muscle, the requirement for these appears to decrease with the activation of multiple overlapping signaling events that are triggered by exercise. This makes exercise a valuable therapeutic tool for the treatment of mitochondrially based metabolic disorders. In this review, we summarize some of the traditional and more recently appreciated pathways that are involved in mitochondrial biogenesis in muscle, particularly during exercise

Marichromatium bheemlicum sp. nov., a non-diazotrophic photosynthetic gammaproteobacterium from a marine aquaculture pond

A rod-shaped, phototrophic, purple sulfur bacterium, strain JA124(T), was isolated in pure culture from a marine aquaculture pond, located near Bhimunipatnam, in a medium that contained 3 % NaCl (w/v). Strain JA124(T) is a Gram-negative, motile rod with a single polar flagellum. Strain JA124(T) has a requirement for NaCl, with optimum growth at 1.5-8.5 %, and tolerates up to 11 % NaCl. Intracellular photosynthetic membranes are of the vesicular type. Bacteriochlorophyll a and probably carotenoids of the spirilloxanthin series are present as photosynthetic pigments. Strain JA124(T) was able to utilize sulfide, sulfate, thiosulfate, sulfite, thioglycollate and cysteine as sulfur sources. Strain JA124(T) was able to grow photolithoautotrophically, photolithoheterotrophically and photo-organoheterotrophically. Chemotrophic and fermentative growth could not be demonstrated. Strain JA124(T) lacks diazotrophic growth and acetylene reduction activity. Pyridoxal phosphate is required for growth. During growth on reduced sulfur sources as electron donors, sulfur is deposited intermediately as a number of small granules within the cell. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain JA124(T) clusters with species of the genus Marichromatium belonging to the class Gammaproteobacteria. The highest sequence similarities of strain JA124(T) were found with the type strains of Marichromatium indicum (98 %), Marichromatium purpuratum (95 %) and Marichromatium gracile (93 %). However, DNA-DNA hybridization with Marichromatium indicum DSM 15907(T) revealed relatedness of only 65 % with strain JA124(T). The DNA base composition of strain JA124(T) was 67 mol% G+C (by HPLC). Based on 16S rRNA gene sequence analysis, morphological and physiological characteristics and DNA-DNA hybridization studies, strain JA124(T) (=ATCC BAA-1316(T)=JCM 13911(T)) is sufficiently different from other Marichromatium species to merit its description as the type strain of a novel species, Marichromatium bheemlicum sp. nov

Haloalkaliphilic spore-forming sulfidogens from soda lake sediments and description of Desulfitispora alkaliphila gen. nov., sp. nov.

An anaerobic enrichment with pyruvate as electron donor and thiosulfate at pH 10 and 0.6 M Na+ inoculated with pasteurized soda lake sediments resulted in a sulfidogenic coculture of two morphotypes of obligately anaerobic haloalkaliphilic endospore-forming clostridia, which were further isolated in pure culture. Strain AHT16 was a thin long rod able to ferment sugars and pyruvate and to respire H2, formate and pyruvate using thiosulfate and fumarate as electron acceptors and growing optimally at pH 9.5. Thiosulfate was reduced incompletely to sulfide and sulfite. The strain was closely related (99% sequence similarity) to a peptolytic alkaliphilic clostridium Natronincola peptidovorans. Strain AHT17 was a short rod with a restricted respiratory metabolism, growing with pyruvate and lactate as electron donor and sulfite, thiosulfate and elemental sulfur as electron acceptors with a pH optimum 9.5. Thiosulfate was reduced completely via sulfite to sulfide. The ability of AHT17 to use sulfite explained the stability of the original coculture of the two clostridia—one member forming sulfite from thiosulfate and another consuming it. Strain AHT17 formed an independent deep phylogenetic lineage within the Clostridiales and is proposed as a new genus and species Desulfitisporum alkaliphilum gen. nov., sp. nov. (=DSM 22410T = UNIQEM U794T)

Rubrivivax benzoatilyticus sp.nov., an aromatic hydrocarbon-degrading purple betaproteobacterium

A brown-coloured bacterium was isolated from photoheterotrophic (benzoate) enrichments of flooded paddy soil from Andhra Pradesh, India. On the basis of 16S rRNA gene sequence analysis, strain JA2(T) was shown to belong to the class Betaproteobacteria, related to Rubrivivax gelatinosus (99 % sequence similarity). Cells of strain JA2(T) are Gram-negative, motile rods with monopolar single flagella. The strain contained bacteriochlorophyll a and most probably the carotenoids spirilloxanthin and sphaeroidene, but did not have internal membrane structures. Intact cells had absorption maxima at 378, 488, 520, 590, 802 and 884 nm. No growth factors were required. Strain JA2(T) grew on benzoate, 2-aminobenzoate (anthranilate), 4-aminobenzoate, 4-hydroxybenzoate, phthalate, phenylalanine, trans-cinnamate, benzamide, salicylate, cyclohexanone, cyclohexanol and cyclohexane-2-carboxylate as carbon sources and/or electron donors. The DNA G+C content was 74.9 mol%. Based on DNA-DNA hybridization studies, 16S rRNA gene sequence analysis and morphological and physiological characteristics, strain JA2(T) is different from representatives of other photosynthetic species of the Betaproteobacteria and was recognised as representing a novel species, for which the name Rubrivivax benzoatilyticus sp. nov. is proposed. The type strain is JA2(T) (=ATCC BAA-35(T)=JCM 13220(T)=MTCC 7087(T))