The use of digital techniques to examine the intermittent region of a turbulent jet

Voltage signals, sampled at a high rate in the intermittent region of a round jet, are analyzed to provide instantaneous velocity vector information and measures of the vorticity and dissipation scales. A clustering routine to assess the feasibility of using the voltage readings to define the vortical, nonvortical state of the flow is also utilized. The results indicate that the clustering routine is partially successful; more sophisticated discrimination techniques will be required for a complete specification

Circadian systems:different levels of complexity

After approximately 50 years of circadian research, especially in selected circadian model systems (Drosophila, Neurospora, Gonyaulax and, more recently, cyanobacteria and mammals), we appreciate the enormous complexity of the circadian programme in organisms and cells, as well as in physiological and molecular circuits. Many of our insights into this complexity stem from experimental reductionism that goes as far as testing the interaction of molecular clock components in heterologous systems or in vitro. The results of this enormous endeavour show circadian systems that involve several oscillators, multiple input pathways and feedback loops that contribute to specific circadian qualities but not necessarily to the generation of circadian rhythmicity. For a full appreciation of the circadian programme, the results from different levels of the system eventually have to be put into the context of the organism as a whole and its specific temporal environment. This review summarizes some of the complexities found at the level of organisms, cells and molecules, and highlights similar strategies that apparently solve similar problems at the different levels of the circadian system

Summary of Space Environment Magnetometer and Particle Replacement Experiment (SEMPRE) Study

As part of the GOES-R series follow on architecture study following the NOAA Satellite Observing System Architecture (NSOSA) study, a study team evaluated the feasibility of accommodating the GOES in-situ instruments (Magnetometer and Particle Detectors) on a dedicated spacecraft with no impact to the overall baseline mission cost assuming two large observatories. The accommodations cost on a primary operational type observatory are non-negligible requiring: a large non-magnetic boom to reduce the impact of the spacecraft interference on the magnetometer; and strict contamination control and magnetic cleanliness to prevent magnetic contamination near the magnetometers. These, along with the additional interface complexities greatly increase the cost of larger spacecraft by extending integration time with a large marching army. By contrast, a dedicated mission provides flexibility in location and refresh rate not afforded when these sensors are launched as secondary payloads. This study performed an informal industry survey of small form-factor instruments currently flying or in process of being developed. The study identified three potential particle detector suites and multiple magnetometers that will satisfy the requirements while having low enough volume and mass to allow accommodation on a rideshare class spacecraft. Using the largest of the identified particle detector suites, the Goddard Space Flight Center Mission Design Lab developed a design for a rideshare spacecraft that will accommodate the particle detector suite and magnetometer. The cost of the spacecraft, based on multiple cost models, is comparable to the cost of accommodating the magnetometer and particle detector suite on two (East and West) larger main observatories

Prolonged quiescence delays somatic stem cell-like divisions in Caenorhabditis elegans and is controlled by insulin signaling

Cells can enter quiescence in adverse conditions and resume proliferation when the environment becomes favorable. Prolonged quiescence comes with a cost, reducing the subsequent speed and potential to return to proliferation. Here, we show that a similar process happens during Caenorhabditis elegans development, providing an in vivo model to study proliferative capacity after quiescence. Hatching under starvation provokes the arrest of blast cell divisions that normally take place during the first larval stage (L1). We have used a novel method to precisely quantify each stage of postembryonic development to analyze the consequences of prolonged L1 quiescence. We report that prolonged L1 quiescence delays the reactivation of blast cell divisions in C. elegans, leading to a delay in the initiation of postembryonic development. The transcription factor DAF‐16/FOXO is necessary for rapid recovery after extended arrest, and this effect is independent from its role as a suppressor of cell proliferation. Instead, the activation of DAF‐16 by decreased insulin signaling reduces the rate of L1 aging, increasing proliferative potential. We also show that yolk provisioning affects the proliferative potential after L1 arrest modulating the rate of L1 aging, providing a possible mechanistic link between insulin signaling and the maintenance of proliferative potential. Furthermore, variable yolk provisioning in embryos is one of the sources of interindividual variability in recovery after quiescence of genetically identical animals. Our results support the relevance of L1 arrest as an in vivo model to study stem cell‐like aging and the mechanisms for maintenance of proliferation potential after quiescence.Spanish Ministerio de Economía y Competitividad (BFU2016-74949-P and BFU2012- 35509)European Research Council (ERC-2011-StG-281691)Marie-Curie Intra-European Fellowship (FP7-PEOPLE-2013- IEF/GA Nr: 627263

How Temperature Changes Reset a Circadian Oscillator

Circadian rhythms control many physiological activities. The environmental entrainment of rhythms involves the immediate responses of clock components. Levels of the clock protein FRQ were measured in Neurospora at various temperatures; at higher temperatures, the amount of FRQ oscillated around higher levels. Absolute FRQ amounts thus identified different times at different temperatures, so temperature shifts corresponded to shifts in clock time without immediate synthesis or turnover of components. Moderate temperature changes could dominate light-to-dark shifts in the influence of circadian timing. Temperature regulation of clock components could explain temperature resetting of rhythms and how single transitions can initiate rhythmicity from characteristic circadian phases

Characteristics of hypervelocity impact craters on LDEF experiment S1003 and implications of small particle impacts on reflective surfaces

The Ion Beam textured and coated surfaces EXperiment (IBEX), designated S1003, was flown on LDEF at a location 98 deg in a north facing direction relative to the ram direction. Thirty-six diverse materials were exposed to the micrometeoroid (and some debris) environment for 5.8 years. Optical property measurements indicated no changes for almost all of the materials except S-13G, Kapton, and Kapton-coated surfaces, and these changes can be explained by other environmental effects. From the predicted micrometeoroid flux of NASA SP-8013, no significant changes in optical properties of the surfaces due to micrometeoroids were expected. There were hypervelocity impacts on the various diverse materials flown on IBEX, and the characteristics of these craters were documented using scanning electron microscopy (SEM). The S1003 alumigold-coated aluminum cover tray was sectioned into 2 cm x 2 cm pieces for crater documentation. The flux curve generated from this crater data fits well between the 1969 micrometeoroid model and the Kessler debris model for particles less than 10(exp -9) gm which were corrected for the S1003 positions (98 deg to ram). As the particle mass increases, the S1003 impact data is greater than that predicted by even the debris model. This, however, is consistent with data taken on intercostal F07 by the Micrometeoroid/Debris Special Investigating Group (M/D SIG). The mirrored surface micrometeoroid detector flown on IBEX showed no change in solar reflectance and corroborated the S1003 flux curve, as well as results of this surface flown on SERT 2 and OSO 3 for as long as 21 years

A lunar space station

A concept for a space station to be placed in low lunar orbit in support of the eventual establishment of a permanent moon base is proposed. This space station would have several functions: (1) a complete support facility for the maintenance of the permanent moon base and its population; (2) an orbital docking area to facilitate the ferrying of materials and personnel to and from Earth; (3) a zero gravity factory using lunar raw materials to grow superior GaAs crystals for use in semiconductors and mass produce inexpensive fiber glass; and (4) a space garden for the benefit of the air food cycles. The mission scenario, design requirements, and technology needs and developments are included as part of the proposal

Light reception and circadian behavior in `blind' and `clock-less' mutants of <i>Neurospora crassa</i>

The filamentous fungus Neurospora crassa is a model organism for the genetic dissection of blue light photoreception and circadian rhythms. WHITE COLLAR-1 (WC-1) and WC-2 are considered necessary for all light responses, while FREQUENCY (FRQ) is required for light-regulated asexual development (conidia formation); without any of the three, self-sustained (circadian) rhythmicity in constant conditions fails. Here we show that light-regulated and self-sustained development occur in the individual or mutant white collar strains. These strains resemble wild type in their organization of the daily bout of light-regulated conidiation. Molecular profiles of light-induced genes indicate that the individual white collar-1 and white collar-2 mutants utilize distinct pathways, despite their similar appearance in all aspects. Titration of fluence rate also demonstrates different light sensitivities between the two strains. The data require the existence of an as-yet-unidentified photoreceptor. Furthermore, the extant circadian clock machinery in these mutant strains supports the notion that the circadian system in Neurospora involves components outside the WC-FRQ loop

Circadian regulation of olfaction and an evolutionarily conserved, nontranscriptional marker in Caenorhabditis elegans

Circadian clocks provide a temporal structure to processes from gene expression to behavior in organisms from all phyla. Most clocks are synchronized to the environment by alternations of light and dark. However, many organisms experience only muted daily environmental cycles due to their lightless spatial niches (e.g., caves or soil). This has led to speculation that they may dispense with the daily clock. However, recent reports contradict this notion, showing various behavioral and molecular rhythms in Caenorhabditis elegans and in blind cave fish. Based on the ecology of nematodes, we applied low-amplitude temperature cycles to synchronize populations of animals through development. This entrainment regime reveals rhythms on multiple levels: in olfactory cued behavior, in RNA and protein abundance, and in the oxidation state of a broadly conserved peroxiredoxin protein. Our work links the nematode clock with that of other clock model systems; it also emphasizes the importance of daily rhythms in sensory functions that are likely to impact on organism fitness and population structure