Sorption competition and kinetics of trivalent cations (Eu, Y and Cm) on corundum (alpha-Al2O3 ) : A batch sorption and TRLFS study

Abstract In this study we have combined batch sorption and laser spectroscopic investigations to study the sorption of Eu(III) and Cm(III), on the aluminum oxide corundum in single- and multi-metal systems. Experiments were performed using a constant equilibrium time as a function of pH (pH-edges) or at constant pH as a function of equilibrium time (kinetic experiments) in 0.01 M NaClO4 and carbonate free conditions. The objective was to investigate how the sorption behavior of trivalent actinides and lanthanides is affected by the presence of another trivalent metal, Y(III). Our hypothesis was that the addition of higher concentrations of trivalent Y(III) together with a chemically similar trivalent metal, Eu(III) or Cm(III), would affect the sorption behavior of that metal. Batch experiments show that when the concentration of competing Y(III) is high enough (1 × 10−4 M) to occupy most of the surface sites, there is a clear shift in the position of the Eu(III) pH-edge to higher pH. Spectroscopic studies using time-resolved laser fluorescence spectroscopy (TRLFS) clearly confirm sorption competition between the trivalent metals Cm(III) and Y(III), but they also indicate a change in the surface speciation of the trivalent actinide in the presence of the competing metal if the concentration of that competing metal is high enough.Peer reviewe

Dendroclimatic transfer functions revisited : Little Ice Age and Medieval Warm Period summer temperatures reconstructed using artificial neural networks and linear algorithms

Peer reviewe

Examining relational social ontologies of disaster resilience: lived experiences from India, Indonesia, Nepal, Chile and Andean territories

Purpose: The neoliberal resilience discourse and its critiques both contribute to its hegemony, obscuring alternative discourses in the context of risk and uncertainties. Drawing from the “ontology of potentiality”, the authors suggest reclaiming “resilience” through situated accounts of the connected and relational every day from the global south. To explore alternate possibilities, the authors draw attention to the social ontology of disaster resilience that foregrounds relationality, intersectionality and situated knowledge. / Design/methodology/approach: Quilting together the field work experiences in India, Indonesia, Nepal, Chile and Andean territories, the authors interrogate the social ontologies and politics of resilience in disaster studies in these contexts through six vignettes. Quilting, as a research methodology, weaves together various individual fragments involving their specific materialities, situated knowledge, layered temporalities, affects and memories. The authors’ six vignettes discuss the use, politicisation and resistance to resilience in the aftermath of disasters. / Findings: While the pieces do not try to bring out a single “truth”, the authors argue that firstly, the vignettes provide non-Western conceptualisations of resilience, and attempts to provincialise externally imposed notions of resilience. Secondly, they draw attention to social ontology of resilience as the examples underscores the intersubjectivity of disaster experiences, the relational reaching out to communities and significant others. / Originality/value: Drawing from in-depth research conducted in six disaster contexts by seven scholars from South Asia, South America and Northern Europe, the authors embrace pluralist situated knowledge, and cross-cultural/language co-authoring. Thus, the co-authored piece contributes to diversifying disaster studies scholarship methodologically

OSBP-related protein-2 (ORP2) : a novel Akt effector that controls cellular energy metabolism

ORP2 is a ubiquitously expressed OSBP-related protein previously implicated in endoplasmic reticulum (ER)lipid droplet (LD) contacts, triacylglycerol (TG) metabolism, cholesterol transport, adrenocortical steroidogenesis, and actin-dependent cell dynamics. Here, we characterize the role of ORP2 in carbohydrate and lipid metabolism by employing ORP2-knockout (KO) hepatoma cells (HuH7) generated by CRISPR-Cas9 gene editing. The ORP2-KO and control HuH7 cells were subjected to RNA sequencing, analyses of Akt signaling, carbohydrate and TG metabolism, the extracellular acidification rate, and the lipidome, as well as to transmission electron microscopy. The loss of ORP2 resulted in a marked reduction of active phosphorylated Akt(Ser473) and its target Glycogen synthase kinase 3(Ser9), consistent with defective Akt signaling. ORP2 was found to form a physical complex with the key controllers of Akt activity, Cdc37, and Hsp90, and to co-localize with Cdc37 and active Akt(Ser473) at lamellipodial plasma membrane regions, in addition to the previously reported ER-LD localization. ORP2-KO reduced glucose uptake, glycogen synthesis, glycolysis, mRNA-encoding glycolytic enzymes, and SREBP-1 target gene expression, and led to defective TG synthesis and storage. ORP2-KO did not reduce but rather increased ER-LD contacts under basal culture conditions and interfered with their expansion upon fatty acid loading. Together with our recently published work (Kentala et al. in FASEB J 32:1281-1295, 2018), this study identifies ORP2 as a new regulatory nexus of Akt signaling, cellular energy metabolism, actin cytoskeletal function, cell migration, and proliferation.Peer reviewe

Water quality monitoring in the former Soviet Union and the Russian Federation : Assessment of analytical methods

Monitoring of surface water quality in the former Soviet Union (FSU) and the present-day Russian Federation historically held an important place in the hierarchy of science, legal framework and relations between agencies. Sadly, the gap between the intentions, qualification of managers and effective programmes has always been sizeable. Since disintegration of the FSU this gap has become a formidable barrier for collecting reliable monitoring information and producing effective water quality management decisions in the Russian Federation. Updating the federal system for freshwater quality monitoring in the Russian Federation is complicated by several unresolved problems. The principal issues are political, technical, institutional and financial. The existing Russian model of water chemistry data collection inherited from the FSU has proved unreliable, outdated and unrelated to modern national issues of water management. The quality of produced data is one of the greatest weaknesses of the federal monitoring system both in the Russian Federation and in other states of the FSU. A significant cause of the low reliability of the produced information is the analytical methods used in monitoring, their inappropriate use, non-compliance to laboratory practices when following expert recommendations, insufficient training level of managers and laboratory personnel and under-funding of the federal monitoring system. The growing national priorities in the field of surface water quality control and improvement conflict with the capacity of the Russian Federation to provide necessary information of guaranteed high quality. Here we make the first attempt to present a critical analysis of the analytical methods used to assess and control surface water quality, to show the main errors arising when applying the recommended analytical methods, and to assess the degree of reliability of produced monitoring information from 1977-1978 and to the present. Our overall objective is to summarize the current situation in order to facilitate implementation of future improvements

Clinical practice selectively follows acute appendicitis guidelines

Peer reviewe

Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum

Thiolase is the first enzyme catalysing the condensation of two acetyl-coenzyme A (CoA) molecules to form acetoacetyl-CoA in a dedicated pathway towards the biosynthesis of n-butanol, an important solvent and biofuel. Here we elucidate the crystal structure of Clostridium acetobutylicum thiolase (CaTHL) in its reduced/oxidized states. CaTHL, unlike those from other aerobic bacteria such as Escherichia coli and Zoogloea ramegera, is regulated by the redox-switch modulation through reversible disulfide bond formation between two catalytic cysteine residues, Cys88 and Cys378. When CaTHL is overexpressed in wild-type C. acetobutylicum, butanol production is reduced due to the disturbance of acidogenic to solventogenic shift. The CaTHLV77Q/N153Y/A286K mutant, which is not able to form disulfide bonds, exhibits higher activity than wild-type CaTHL, and enhances butanol production upon overexpression. On the basis of these results, we suggest that CaTHL functions as a key enzyme in the regulation of the main metabolism of C. acetobutylicum through a redox-switch regulatory mechanism.close0

Quality of life and metabolic status in mildly depressed women with type 2 diabetes treated with paroxetine: A single-blind randomised placebo controlled trial

BACKGROUND: Depression is prevalent in people with type 2 diabetes and affects both glycemic control and overall quality of life. The aim of this trial was to evaluate the effect of the antidepressant paroxetine on metabolic control, quality of life and mental well-being in mildly depressed women with type 2 diabetes. METHODS: We randomised 15 mildly depressed women with non-optimally controlled type 2 diabetes to a 10-week single-blind treatment with either paroxetine 20 mg per day or placebo. Primary efficacy measurements were glycemic control and quality of life. Glycosylated hemoglobin A(1c )(GHbA(1c)) was used as a measure of glycemic control. Quality of life was evaluated using RAND-36. Mental state was assessed using two clinician-rated scoring instruments, Hamilton's Anxiety Scale (HAM-A) and Montgomery-Åsberg's Depression Rating Scale (MADRS), and a patient-rated scoring instrument, Beck's Depression Inventory (BDI). RESULTS: At the end of the study no significant difference between groups in improvement of quality of life was found. A trend towards a superior improvement in glycemic control was found in the paroxetine group (p = 0.08). A superior increase in sex-hormone-binding-globuline (SHBG) levels was evidenced in the paroxetine group (p = 0.01) as a sign of improved insulin sensitivity. There was also a trend for superior efficacy of paroxetine in investigator-rated anxiety and depression. This notion was supported by a trend for superior decrease of serum cortisol levels in the paroxetine group (p = 0.06). CONCLUSION: Paroxetine has a beneficial effect on measures of insulin sensitivity and may improve glycemic control. Larger studies of longer duration are needed to verify the benefits of paroxetine in type 2 diabetes. While waiting for more conclusive evidence it seems sensible to augment standard care of type 2 diabetes with paroxetine even in patients who do not fulfil routine psychiatric criteria for initiation of antidepressant drug treatment

Sää- ja ilmastoriskit Suomessa - Kansallinen arvio

Tähän raporttiin on koottu ajantasainen arvio sään ja ilmaston aiheuttamista riskeistä eri toimialoille Suomessa. Arviossa otettiin huomioon sekä muuttuvan ilmaston että yhteiskunnallisen kehityksen vaikutus riskin muodostumiseen nykyhetkessä ja tulevaisuudessa. Sää- ja ilmastoriskejä pyrittiin hahmottamaan vaaratekijän (riskiä aiheuttava sääilmiö), altistumisen (riskin kohteen sijainti) ja haavoittuvuuden (riskin kohteen ominaisuudet) yhdistelmänä. Sääilmiöt aiheuttavat Suomessa riskejä jo nykyilmastossa. Muun muassa rajuilmat, helleaallot ja rankkasateet aiheuttavat taloudellisia ja terveydellisiä vaikutuksia sekä yleistä haittaa. Tulevaisuudessa riskit muuttuvat ilmastonmuutoksen muuttaessa haitallisia sääilmiöitä. Ilmastonmuutos tuo vähitellen kasvavia riskejä erityisesti ekosysteemeille ja infrastruktuurille. Muualla maailmalla tapahtuvat ilmastonmuutoksen vaikutukset voivat heijastua epäsuorasti Suomeen globaalien tavara-, energia-, raha- ja ihmisvirtojen kautta. Näiden riskien systemaattinen arviointi on vasta aloitettu. Raportin tavoitteena on tukea yhteiskunnan riskeihin varautumista ja ilmastonmuutokseen sopeutumista eri hallinnon tasoilla ja toimialoilla. Arvio perustuu pääosin kirjallisuudesta löytyviin tutkimuksiin ja selvityksiin sekä asiantuntija-arvioihin. Työ tehtiin “Sää- ja ilmastoriskien arviointi ja toimintamallit” (SIETO)- hankkeessa vuosina 2017–2018