Germination of grass seeds with recycling waste water

This study was designed to determine the effects of residual water irrigation on the rate and percentage of germination of grass seeds. Germination tests were carried out to compare the seeds irrigated with recycling waste water with seeds irrigated with distilled water. Test with Festuca arundinacea Sch. and Agrostis tenuis L. seeds was performed under laboratory conditions. Parameters used to evaluate germination were: number of germinated seeds (Gmax), mean germination time (MGT), the time required for 1 to 75%, of the seeds to germinate (T1, T10, T25, T50 and T75). The evaluated parameters for both seeds irrigated with recycled waste water were below the control seeds parameters. These reductions involve that germination rate was increased. Recycling waste water could be used for irrigation of grass seeds because produces a beneficial effect in germination rate and percentage

Diagnóstico de necesidades de formación para revitalizar el sector comercial

Producción CientíficaEstudio del comercio minorista en las ciudades de Valladolid (incluyendo análisis pormenorizado del centro y varios barrios) y Medina del Campo, basado en el impuesto de actividades económicas y la realización de encuestas a comerciantes y consumidores.Geografí

Formación de los tutores de las prácticas externas como herramienta eficaz en la mejora del aprendizaje del estudiante y su inserción laboral

El objetivo del proyecto es mejorar la calidad de la tutorización de las prácticas externas y fomentar las buenas prácticas que garanticen el proceso de enseñanza-aprendizaje. Están implicados tutores académicos, externos, instituciones y estudiante

CARB-ES-19 Multicenter Study of Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli From All Spanish Provinces Reveals Interregional Spread of High-Risk Clones Such as ST307/OXA-48 and ST512/KPC-3

ObjectivesCARB-ES-19 is a comprehensive, multicenter, nationwide study integrating whole-genome sequencing (WGS) in the surveillance of carbapenemase-producing K. pneumoniae (CP-Kpn) and E. coli (CP-Eco) to determine their incidence, geographical distribution, phylogeny, and resistance mechanisms in Spain.MethodsIn total, 71 hospitals, representing all 50 Spanish provinces, collected the first 10 isolates per hospital (February to May 2019); CPE isolates were first identified according to EUCAST (meropenem MIC > 0.12 mg/L with immunochromatography, colorimetric tests, carbapenem inactivation, or carbapenem hydrolysis with MALDI-TOF). Prevalence and incidence were calculated according to population denominators. Antibiotic susceptibility testing was performed using the microdilution method (EUCAST). All 403 isolates collected were sequenced for high-resolution single-nucleotide polymorphism (SNP) typing, core genome multilocus sequence typing (cgMLST), and resistome analysis.ResultsIn total, 377 (93.5%) CP-Kpn and 26 (6.5%) CP-Eco isolates were collected from 62 (87.3%) hospitals in 46 (92%) provinces. CP-Kpn was more prevalent in the blood (5.8%, 50/853) than in the urine (1.4%, 201/14,464). The cumulative incidence for both CP-Kpn and CP-Eco was 0.05 per 100 admitted patients. The main carbapenemase genes identified in CP-Kpn were blaOXA–48 (263/377), blaKPC–3 (62/377), blaVIM–1 (28/377), and blaNDM–1 (12/377). All isolates were susceptible to at least two antibiotics. Interregional dissemination of eight high-risk CP-Kpn clones was detected, mainly ST307/OXA-48 (16.4%), ST11/OXA-48 (16.4%), and ST512-ST258/KPC (13.8%). ST512/KPC and ST15/OXA-48 were the most frequent bacteremia-causative clones. The average number of acquired resistance genes was higher in CP-Kpn (7.9) than in CP-Eco (5.5).ConclusionThis study serves as a first step toward WGS integration in the surveillance of carbapenemase-producing Enterobacterales in Spain. We detected important epidemiological changes, including increased CP-Kpn and CP-Eco prevalence and incidence compared to previous studies, wide interregional dissemination, and increased dissemination of high-risk clones, such as ST307/OXA-48 and ST512/KPC-3

El huerto escolar : recurso educativo del geocentro

El trabajo obtuvo un premio de la Modalidad A de los Premios Tomás García Verdejo a las buenas practicas educativas en la Comunidad Autónoma de Extremadura para el curso 2011-2012Se describe un proyecto que consistió en crear un huerto escolar con el que se quería enfatizar la importancia de la protección del medio ambiente y fomentar el respeto y el amor por la naturaleza, valorando su importancia e interdependencia con la vida de los seres humanos. Se detallan las actividades llevadas a cabo dirigidas tanto a alumnos de infantil como de primariaExtremaduraES

Ex vivo, in situ perfusion protocol for human brain fixation compatible with microscopy, MRI techniques, and anatomical studies

We present a method for human brain fixation based on simultaneous perfusion of 4% paraformaldehyde through carotids after a flush with saline. The left carotid cannula is used to perfuse the body with 10% formalin, to allow further use of the body for anatomical research or teaching. The aim of our method is to develop a vascular fixation protocol for the human brain, by adapting protocols that are commonly used in experimental animal studies. We show that a variety of histological procedures can be carried out (cyto- and myeloarchitectonics, histochemistry, immunohistochemistry, intracellular cell injection, and electron microscopy). In addition, ex vivo, ex situ high-resolution MRI (9.4T) can be obtained in the same specimens. This procedure resulted in similar morphological features to those obtained by intravascular perfusion in experimental animals, provided that the postmortem interval was under 10 h for several of the techniques used and under 4 h in the case of intracellular injections and electron microscopy. The use of intravascular fixation of the brain inside the skull provides a fixed whole human brain, perfectly fitted to the skull, with negligible deformation compared to conventional techniques. Given this characteristic of ex vivo, in situ fixation, this procedure can probably be considered the most suitable one available for ex vivo MRI scans of the brain. We describe the compatibility of the method proposed for intravascular fixation of the human brain and fixation of the donor’s body for anatomical purposes. Thus, body donor programs can provide human brain tissue, while the remainder of the body can also be fixed for anatomical studies. Therefore, this method of human brain fixation through the carotid system optimizes the procurement of human brain tissue, allowing a greater understanding of human neurological diseases, while benefiting anatomy departments by making the remainder of the body available for teaching purposes.This work was supported in part by the National Institute of Health (Grant R01 AG056014-R01), a UCLM travel grant (to RI), a UCLM research grant to Human Neuroanatomy Laboratory (2020-GRIN-28837), and project EQC2019-006341-P (AEI/FEDER UE)

La Atención a las Necesidades en Salud Mental de los Profesionales Sanitarios durante la COVID-19

Los profesionales sanitarios se encuentran en primera línea de atención a pacientes con COVID-19, presentando mayor riesgo de contagio y de afectación emocional. El objetivo del estudio es describir las necesidades en salud mental y conocer el efecto de una intervención psicológica en estos profesionales. Se incluyeron 47 trabajadores del Hospital Universitari Vall d’Hebron que solicitaron soporte psicológico durante el primer mes y medio de la crisis. Se administraron pre-post intervención un listado de síntomas clínicos, la Escala Clínica de Ansiedad y de Impresión Clínica Global. Consultaron mayoritariamente mujeres, profesionales de enfermería y técnicos auxiliares de enfermería, presentando hiperpreocupación, tristeza, alteraciones de sueño y orexia. Se observó una mejoría significativa en síntomas clínicos (z = 4.6, p ≤ .0001), estrés agudo (z = 2.5, p = .012), ansiedad (z = 5.3, p ≤ .0001) e impresión clínica (z = 4.1, p ≤ .0001). Una intervención psicológica breve, basada en técnicas de gestión de ansiedad, regulación emocional y orientación en valores reduce los síntomas emocionales en profesionales

Unfolding the Medial Temporal Lobe Cortex to Characterize Neurodegeneration Due to Alzheimer’s Disease Pathology Using Ex vivo Imaging

Neurofibrillary tangle (NFT) pathology in the medial temporal lobe (MTL) is closely linked to neurodegeneration, and is the early pathological change associated with Alzheimer’s Disease (AD). In this work, we investigate the relationship between MTL morphometry features derived from high-resolution ex vivo imaging and histology-based measures of NFT pathology using a topological unfolding framework applied to a dataset of 18 human postmortem MTL specimens. The MTL has a complex 3D topography and exhibits a high degree of inter-subject variability in cortical folding patterns which poses a significant challenge for volumetric registration methods typically used during MRI template construction. By unfolding the MTL cortex, the proposed framework explicitly accounts for the sheet-like geometry of the MTL cortex and provides a two-dimensional reference coordinate space which can be used to implicitly register cortical folding patterns across specimens based on distance along the cortex despite large anatomical variability. Leveraging this framework in a subset of 15 specimens, we characterize the associations between NFTs and morphological features such as cortical thickness and surface curvature and identify regions in the MTL where patterns of atrophy are strongly correlated with NFT pathology