4 research outputs found
Evaluation of E-cadherin (CDH1) gene polymorphism related to gastric cancer in Kurdish population
Abstract: Helicobacter pylori (H.pylori) infection induces inflammation in gastric mucosa that may progress to
gastric cancer that causes of much mortality. This cancer is a multistage process involved changes in environmental,
genetic and epigenetic factors. Polymorphism in promoter of CDH1 gene is associated with reduced E-cadherin
protein expression. Gastric cancer is associated with multiple changes nucleotides in CDH1 gene. Aimed: We were
evaluating -160 (C>A) CDH1 gene polymorphism associations with gastric cancer in Kurdish population. Methods:
A total of 306 biopsies taken from corpus of 144 gastric cancer patients and 162 nonulcer dyspepsia patients were
classified as H.pylori-infected and H.pylori-uninfected. All diagnoses confirmed pathologically and molecularly.
Polymorphism in -160(C>A) CDH1 was evaluated by PCR-RFLP. Results: Polymorphism of -160 (C>A) CDH1 in
H.pylori-uninfected and H.pylori-infected groups were not associated with gastric cancer (p > 0.05). Also there was
not relationship between -160(C>A) CDH1 genotypes and H.pylori infection susceptibility (p > 0.05). We found
significant relationship between CC genotype and survival time among gastric cancer patients (p = 0.01).
Conclusion: -160(C>A) CDH1 polymorphism may regardless of presence or absence of H.pylori, don’t influences
gastric cancer sensibility in Kurdish population. In other hand CC genotype, as a good trait, increases period of life
for Kurdish cancer patients
Evaluation of IL-17A and IL-17F genes polymorphism in Iranian dyspeptic patients
Helicobacter pylori (H.pylori) colonize the gastric mucosa of approximately 50 of the world's population that involved in chronic gastritis. The relationship between Hp colonization and gastric inflammation is widely accepted. Polymorphisms in inflammation related genes such as cytokines were thought to partly determine the outcome of Hp infection and progression of gastritis. Interleukin IL -17A and IL-17F are inflammatory cytokines expressed by a novel subset of CD4+ Th cells, play important function in inflammation. Aimed: we evaluate association of IL-17A G197A and IL-17F A7488G polymorphisms with gastritis, Polymorphonuclear (PMN) and Monoculear (MN) infiltration in related to Hp. Methods: According to rapid urease test, PCR 16srRNA, urea and histological examination of biopsies, patients were classified Hp-infected and Hp-uninfected. The histological severity of gastritis was graded from normal to severe based on the degree of MN cell and PMN leukocyte infiltration, chronic gastritis and chronic active gastritis. Polymorphism in IL-17A G197A and IL-17F A7488G were evaluated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: AG, GG, AG/AA carriers of IL-17A G197A and AA, GA, GG, GA/GG carriers of IL-17F A7488G polymorphisms were not associated with MN infiltration, PMN infiltration, chronic gastritis and Chronic active gastritis in Hp-infected and Hp-uninfected groups (p > 0.05). AA genotype of IL-17A G197A was related to chronic gastritis and PMN infiltration in Hp-uninfected group. Conclusion: IL-17A G197A substitution may be a risk factor for development gastritis in Hp-uninfected patients, also affect the pathway MN cell production pathways
Higher risk of progressing breast cancer in Kurdish population associated to CDH1 -160 C/A polymorphism
There is an increasing interest about studying possible effects of genetic polymorphisms and risk
of cancer pro- gression. E-cadherin (CDH1) involves in many important cellular processes including
cell-cell interactions, cell development and genetic changes of this molecule has been associated
with greater tumor metastasis. The present study was aimed to evaluate the possible role of CDH1
-160 C/A polymorphism as a potential risk factor for breast cancer in Kurdish population. This
case-control study consisted of 100 breast cancer patients and 200 healthy controls.
Clinicopathological findings of all individuals were reported and immunohistochemistry staining was
carried out on tissue samples. The CDH1 -160 C/A genotype was determined by polymerase chain
reaction- re- striction fragment length polymorphism method (PCR-RFLP). CDH1 -160 C/A polymorphism
was differently distributed between patient and control groups. The A allele of CDH1 -160 C/A
polymorphism significantly in- creased in patients compared to controls. In addition we found that
the A allele of this polymorphism might be a potential risk factor for progression of breast cancer
in our studied population. Patients with A allele of CDH1 -160 C/A was in higher risk to
progress invasive ductal carcinoma. The A allele was also correlated with high grade and stage IV
and also with metastatic tumors in studied subjects. The CDH1 -160 C/A polymorphism is correlated
with clinicopathologial findings of breast cancer patients. The A allele of CDH1 -160 C/A may be a
risk factor for progression of breast cancer in Kurdish patients