A native promoter–gene fusion created by CRISPR/Cas9-mediated genomic deletion offers a transgene-free method to drive oil accumulation in leaves

Achieving gain-of-function phenotypes without inserting foreign DNA is an important challenge for plant biotechnologists. Here we show that a gene can be brought under the control of a promoter from an upstream gene by deleting the intervening genomic sequence using dual-guide CRISPR/Cas9. We fuse the promoter of a non-essential photosynthesis-related gene to DIACYLGLYCEROL ACYLTRANSFERASE 2 (DGAT2) in the lipase-deficient sugar-dependent 1 mutant of Arabidopsis thaliana to drive ectopic oil accumulation in leaves. DGAT2 expression is enhanced more than twenty-fold and the triacylglycerol content increases by around thirty-fold. This deletion strategy offers a transgene-free route to engineering traits that rely on transcriptional gain-of-function, such as producing high lipid forage to increase the productivity and sustainability of ruminant farming

Microlensing of Circumstellar Disks

We investigate the microlensing effects on a source star surrounded by a circumstellar disk, as a function of wavelength. The microlensing light curve of the system encodes the geometry and surface brightness profile of the disk. In the mid- and far-infrared, the emission of the system is dominated by the thermal emission from the cold dusty disk. For a system located at the Galactic center, we find typical magnifications to be of order 10-20% or higher, depending on the disk surface brightness profile, and the event lasts over one year. At around 20 microns, where the emission for the star and the disk are comparable, the difference in the emission areas results in a chromatic microlensing event. Finally, in the near-infrared and visible, where the emission of the star dominates, the fraction of star light directly reflected by the disk slightly modifies the light curve of the system which is no longer that of a point source. In each case, the corresponding light curve can be used to probe some of the disk properties. A fraction of 0.1% to 1% optical microlensing events are expected to be associated with circumstellar disk systems. We show that the lensing signal of the disk can be detected with sparse follow-up observations of the next generation space telescopes. While direct imaging studies of circumstellar disks are limited to the solar neighborhood, this microlensing technique can probe very distant disk systems living in various environments and has the potential to reveal a larger diversity of circumstellar disks.Comment: 9 pages, 7 figures. Accepted for publication in Ap

[OI] disk emission in the Taurus star forming region

The structure of protoplanetary disks is thought to be linked to the temperature and chemistry of their dust and gas. Whether the disk is flat or flaring depends on the amount of radiation that it absorbs at a given radius, and on the efficiency with which this is converted into thermal energy. The understanding of these heating and cooling processes is crucial to provide a reliable disk structure for the interpretation of dust continuum emission and gas line fluxes. Especially in the upper layers of the disk, where gas and dust are thermally decoupled, the infrared line emission is strictly related to the gas heating/cooling processes. We aim to study the thermal properties of the disk in the oxygen line emission region, and to investigate the relative importance of X-ray (1-120 Angstrom) and far-UV radiation (FUV, 912-2070 Angstrom) for the heating balance there. We use [OI] 63 micron line fluxes observed in a sample of protoplanetary disks of the Taurus/Auriga star forming region and compare it to the model predictions presented in our previous work. The data were obtained with the PACS instrument on board the Herschel Space Observatory as part of the Herschel Open Time Key Program GASPS (GAS in Protoplanetary diskS), published in Howard et al. (2013). Our theoretical grid of disk models can reproduce the [OI] absolute fluxes and predict a correlation between [OI] and the sum Lx+Lfuv. The data show no correlation between the [OI] line flux and the X-ray luminosity, the FUV luminosity or their sum. The data show that the FUV or X-ray radiation has no notable impact on the region where the [OI] line is formed. This is in contrast with what is predicted from our models. Possible explanations are that the disks in Taurus are less flaring than the hydrostatic models predict, and/or that other disk structure aspects that were left unchanged in our models are important. ..abridged..Comment: 9 pages, accepted for publication in A&

Confinement effects on glass forming liquids probed by DMA

Many molecular glass forming liquids show a shift of the glass transition T-g to lower temperatures when the liquid is confined into mesoporous host matrices. Two contrary explanations for this effect are given in literature: First, confinement induced acceleration of the dynamics of the molecules leads to an effective downshift of T-g increasing with decreasing pore size. Second, due to thermal mismatch between the liquid and the surrounding host matrix, negative pressure develops inside the pores with decreasing temperature, which also shifts T-g to lower temperatures. Here we present dynamic mechanical analysis measurements of the glass forming liquid salol in Vycor and Gelsil with pore sizes of d=2.6, 5.0 and 7.5 nm. The dynamic complex elastic susceptibility data can be consistently described with the assumption of two relaxation processes inside the pores: A surface induced slowed down relaxation due to interaction with rough pore interfaces and a second relaxation within the core of the pores. This core relaxation time is reduced with decreasing pore size d, leading to a downshift of T-g proportional to 1/d in perfect agreement with recent differential scanning calorimetry (DSC) measurements. Thermal expansion measurements of empty and salol filled mesoporous samples revealed that the contribution of negative pressure to the downshift of T-g is small (<30%) and the main effect is due to the suppression of dynamically correlated regions of size xi when the pore size xi approaches

Gas and dust in the Beta Pictoris Moving Group as seen by the Herschel Space Observatory

Context. Debris discs are thought to be formed through the collisional grinding of planetesimals, and can be considered as the outcome of planet formation. Understanding the properties of gas and dust in debris discs can help us to comprehend the architecture of extrasolar planetary systems. Herschel Space Observatory far-infrared (IR) photometry and spectroscopy have provided a valuable dataset for the study of debris discs gas and dust composition. This paper is part of a series of papers devoted to the study of Herschel PACS observations of young stellar associations. Aims. This work aims at studying the properties of discs in the Beta Pictoris Moving Group (BPMG) through far-IR PACS observations of dust and gas. Methods. We obtained Herschel-PACS far-IR photometric observations at 70, 100 and 160 microns of 19 BPMG members, together with spectroscopic observations of four of them. Spectroscopic observations were centred at 63.18 microns and 157 microns, aiming to detect [OI] and [CII] emission. We incorporated the new far-IR observations in the SED of BPMG members and fitted modified blackbody models to better characterise the dust content. Results. We have detected far-IR excess emission toward nine BPMG members, including the first detection of an IR excess toward HD 29391.The star HD 172555, shows [OI] emission, while HD 181296, shows [CII] emission, expanding the short list of debris discs with a gas detection. No debris disc in BPMG is detected in both [OI] and [CII]. The discs show dust temperatures in the range 55 to 264 K, with low dust masses (6.6*10^{-5} MEarth to 0.2 MEarth) and radii from blackbody models in the range 3 to 82 AU. All the objects with a gas detection are early spectral type stars with a hot dust component.Comment: 12 pages, 7 figures, 6 table

Sub-percent constraints on cosmological temperature evolution

The redshift dependence of the cosmic microwave background temperature is one of the key cosmological observables. In the standard cosmological model one has T (z) = T0(1 + z), where T0 is the present-day temperature. Deviations from this behavior would imply the presence of new physics. Here we discuss how the combination of all currently available direct and indirect measurements of T (z) constrains the common phenomenological parametrization T (z) = T0(1 + z)^(1−β) , and obtain the first sub-percent constraint on the temperature growth index 1 − β. Specifically, we find β = (7.6 ± 8.0) × 10^(−3) at the 68.3% confidence level

Randomized Clinical Trial of Azithromycin vs. Erythromycin for the Treatment of Chlamydia Cervicitis in Pregnancy

Objective: The purpose of this study was to prospectively test the null hypothesis that there is no difference in the clinical effectiveness of azithromycin and erythromycin for the treatment of chlamydia cervicitis in pregnancy

Growth, Structure and Properties of BiFeO3-BiCrO3 Films obtained by Dual Cross Beam PLD

The properties of epitaxial Bi2FeCrO6 thin films, recently synthesized by pulsed laser deposition, have partially confirmed the theoretical predictions (i.e. a magnetic moment of 2 muB per formula unit and a polarization of ~80 microC/cm2 at 0K). The existence of magnetic ordering at room temperature for this material is an unexpected but very promising result that needs to be further investigated. Since magnetism is assumed to arise from the exchange interaction between the Fe and Cr cations, the magnetic behaviour is strongly dependent on both their ordering and the distance between them. We present here the successful synthesis of epitaxial Bi2FexCryO6 (BFCO x/y) films grown on SrTiO3 substrates using dual crossed beam pulsed laser deposition. The crystal structure of the films has different types of (111)-oriented superstructures depending on the deposition conditions. The multiferroic character of BFCO (x/y) films is proven by the presence of both ferroelectric and magnetic hysteresis at room temperature. The oxidation state of Fe and Cr ions in the films is shown to be 3+ only and the difference in macroscopic magnetization with Fe/Cr ratio composition could only be due to ordering of the Cr3+ and Fe3+ cations therefore to the modification of the exchange interaction between them.Comment: Manuscript accepted for publication in IEEE-UFF