Towards understanding breast cancer mechanisms to metastasize

Accession Number: GSE47389 Platform: GPL570: [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array Organism: Homo sapiens Published on 2013-11-01 Summary: How organ-specific metastatic traits accumulate in primary tumors remains unknown. We identified a role of the primary tumor stroma in selecting breast cancer cells that are primed for metastasis in the bone. A fibroblast-rich stroma in breast tumors creates a microenvironment that is similar to that of bone metastases in its abundance of the cytokines CXCL12 and IGF1. Heterogeneous breast cancer cell populations growing in such mesenchymal environment evolve towards a preponderance of clones that thrive on CXCL12 and IGF1. Fibroblast-driven selection of bone metastatic clones in mammary tumors is suppressed by CXCL12 and IGF1 receptor inhibition. Thus, a fibroblast-rich stroma in breast tumors can pre-select bone metastatic seeds, promoting the evolution of metastatic traits and the interplay between a primary tumor and its distant metastases. Overall Design: Affymetrix U133 Plus2 arrays were hybridized according to the manufacturer's procedure using RNA extracted from 47 primary breast tumors. Specific gene sets were evaluated in this cohort. Contact: Name: Marcel Smid Organization: Erasmus MC Deparment: Medical Oncology Address: Dr. Molewaterplein 50 Rotterdam Netherlands Organization: Affymetrix, Inc. Address: Santa Clara CA 95051 USA Email: [email protected], [email protected] Phone: 888-362-2447 Web-Link: http://www.affymetrix.com/index.aff

Towards understanding breast cancer mechanisms to metastasize

Accession Number: GSE47389 Platform: GPL570: [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array Organism: Homo sapiens Published on 2013-11-01 Summary: How organ-specific metastatic traits accumulate in primary tumors remains unknown. We identified a role of the primary tumor stroma in selecting breast cancer cells that are primed for metastasis in the bone. A fibroblast-rich stroma in breast tumors creates a microenvironment that is similar to that of bone metastases in its abundance of the cytokines CXCL12 and IGF1. Heterogeneous breast cancer cell populations growing in such mesenchymal environment evolve towards a preponderance of clones that thrive on CXCL12 and IGF1. Fibroblast-driven selection of bone metastatic clones in mammary tumors is suppressed by CXCL12 and IGF1 receptor inhibition. Thus, a fibroblast-rich stroma in breast tumors can pre-select bone metastatic seeds, promoting the evolution of metastatic traits and the interplay between a primary tumor and its distant metastases. Overall Design: Affymetrix U133 Plus2 arrays were hybridized according to the manufacturer's procedure using RNA extracted from 47 primary breast tumors. Specific gene sets were evaluated in this cohort. Contact: Name: Marcel Smid Organization: Erasmus MC Deparment: Medical Oncology Address: Dr. Molewaterplein 50 Rotterdam Netherlands Organization: Affymetrix, Inc. Address: Santa Clara CA 95051 USA Email: [email protected], [email protected] Phone: 888-362-2447 Web-Link: http://www.affymetrix.com/index.aff

Transcription profiling of human CD34 cells isolated from cord blood or peripheral blood transduced with AML1-ETO or empty vector (MIGR1)

Human CD34 cells isolated from cord blood or peripheral blood transduced with AML1-ETO or empty vector (MIGR1)