Autochthonous yeast populations from different brazilian geographic indications.

Yeasts are versatile microorganisms which show heterogeneity in their abilities of aromatic molecules formation. The metabolic conversions may improve the production of a particular compound already formed by the microorganism or promote the production of a completely new biochemicals. These conversions depend on the environment. The microbiome of terroir is unique. If the term terroir is a set of physical properties of a vineyard that contribute to the specific characteristics of its wine, the microorganisms will undoubtedly form an integral part of this concept. There are yeasts, filamentous fungi and bacteria that can affect the quality of the wine. The aim of the present study was to identify the autochthonous yeast populations of grape berries collected from regions with Geographic Indications or under construction. The identification was carried out by an approach, combining Maldi-Tof-MS, PCR-RFLP of the internal transcribed spacer with 5.8S ribosomal DNA (rDNA) (ITS1-5.8S-ITS2) and sequences of the D1/D2 domain of the 26S rRNA gene. Some species are common to different GIs and in some of them other species are completely absent, besides some places are contiguous areas. In some areas, Hanseniaspora opuntiae, Saccharomyces cerevisiae, Pichia myanmarensis and Hanseniaspora uvarum were the predominant species.Também publicado em: CONGRESSO MUNDIAL DA VINHA E DO VINHO, 39., 2016, Bento Gonçalves. Programa...Bento Gonçalves, RS: International Organisation of Vine and Wine (OIV): Ministério da Agricultura, Pecuária e Abastecimento, de 19 a 21 outubro, 2016. Artigo nº 7, 02030. 2016

CODAP: um Consenso Multidisciplinar sobre a Definição, Diagnóstico e Tratamento da Dislipidemia Aterogénica em Portugal

Introduction and aims: Atherogenic dyslipidemia is an important contributor to residual cardiovascular (CV) risk, but it is underdiagnosed and undertreated. This study aimed to assess the opinion of Portuguese experts to generate a consensus concerning the diagnosis and treatment of atherogenic dyslipidemia, as well as to contribute toward standardization of clinical practice in this disorder. Methods: The study consisted in the application of a questionnaire to an expert panel, following a modified Delphi methodology. Results: The majority (88.4%) of the proposed items were found to be consensual. The expert panel recognized the importance of the atherogenic dyslipidemia phenotype, the role played by low-density lipoprotein cholesterol and non-high-density lipoprotein cholesterol as risk markers and therapeutic targets, the choice of statins as first-line lipid-lowering drugs, and the value of associating statins with fenofibrate as a means to reduce residual CV risk. However, the role played by triglycerides in CV risk and the therapeutic value of fibrates lacked consensus. Taking into consideration the state of the art and the opinions expressed in this study, the scientific committee developed a treatment algorithm aimed to improve the perception and treatment of atherogenic dyslipidemia. Conclusions: The experts involved in this study were shown to be familiar with the concept and the importance of atherogenic dyslipidemia. The few situations in which a consensus could not be found were mainly related to the interpretation and/or relevance of the available evidence.info:eu-repo/semantics/publishedVersio

Resíduos sólidos de uva e compostos com capacidade oxi-redox favorável.

O objetivo deste estudo foi determinar a diferença existente entre os resíduos dos cultivares Isabel, Niágara, Chardonnay, Pinot Noir e uma mistura de cultivares em relação à concentração dos agentes oxi-redoxf para exploração industrial e comercial.Resumo

Identificação taxonômica de leveduras ambientais por espectrometria de massa MALDI-TOF.

O objetivo deste trabalho foi identificar, por MALDI-TOF MS, 99 leveduras isoladas de uvas cultivadas no Vale do São Francisco/PE para inseri-las na Coleção de Leveduras do Centro Nacional de Pesquisa de Uva e Vinho (WDCM 1056)

Alterações em alguns atributos químicos do solo de cerrado tratado com pó de balão e cultivado com milho, sob condições controladas.

Fertbio 2012

Impact of fungicide residues on polymerase chain reaction and on yeast metabolism.

The indiscriminate use of pesticides on grape crops is harmful for consumers´ healthin “in natura” consumption and in the ingestion of wine and grape juice. During winemaking, a rapid and efficient fermentation stage is critical to avoid proliferation of contaminating microorganisms and to guarantee the product´s quality. Polymerase chain reaction (PCR) has the advantage of detecting these contaminants in the early stages of fermentation. However,this enzymatic reaction may also be susceptible to specific problems, reducing its efficiency. Agricultural practices, such as fungicide treatments, may be a source of PCR inhibiting factors and may also interfere in the normal course of fermentation.The action of the pesticides captan and folpet on PCR and on yeast metabolism was evaluated, once these phthalimide compounds are widely employed in Brazilian vineyards. DNA amplification was only observed at 75 and 37.5 µg/mL of captan concentrations, whereas with folpet, amplification was observed only in the two lowest concentrations tested (42.2 and 21.1µg/mL).Besides the strong inhibition on Taq polymerase activity, phthalimides also inhibited yeast metabolism at all concentrations analyzed.Grape must containing captan and folpet residues could not be transformed into wine due to stuck fermentation caused by the inhibition of yeast metabolism. Noncompliance with the waiting period for phthalimide fungicides may result in financial liabilities to the viticulture sector.The use of yeasts with high fungicide sensitivity should be selected for must fermentation as a strategy for sustainable wine production and to assure that products comply with health and food safety standards. Key words: fungicide;PCR inhibitors; stuck fermentation;Taq polymerase inhibitors; yeast growt

Carotenoids, sugars and dry matter concentrations in sweetpotato are different in two Brazilian regions.

Considering that the expression of sweetpotato characters may vary according to the environment, we aimed to evaluate the nutritional performance of nine genotypes in two different regions. Experiments were carried out in Itabaiana, SE and Gama, DF, in 2015/2015 and 2015/2016 crop years. The experimental design was a four-replicate randomized block design of 10 plants per row, spaced 75 cm between rows and 40 cm between plants. The treatments were the Beauregard cultivar and eight clones 6, 8, 9, 33, 50, 66, 75, 79; in addition, two local cultivars (Olho Roxo and Ourinhos) were used as controls for sugar analysis. They were evaluated for dry matter, total carotenoid and betacarotene content; and the content of fructose, glucose, sucrose, and total soluble solids. Total and soluble sugars evaluations were performed only in 2015/2016. The resulting data underwent analysis of individual and pool variances, simple correlation, and grouping of treatment means by a Scott-Knott test. It was verified that dry matter, brix, total carotenoids, betacarotene, and sugar (fructose and glucose) levels were different in Itabaiana-SE and Gama-DF evaluations. These findings demonstrate the importance of regional validations of sweetpotato quality traits under different Brazilian conditions

Coleção de microrganismos de interesse agroindustrial: histórico e realizações.

Esta publicação trata do histórico da Coleção de Microrganismos de Interesse Industrial (CMIA), pertencente à Embrapa Uva e Vinho, lotada em Bento Gonçalves, que teve sua origem em 1980, como um aglomerado de leveduras. Com o aumento progressivo do número de leveduras e depois de estruturado, esse conjunto se tornou uma Coleção Institucional com normas e regras bem definidas, obedecendo as diretrizes do Sistema de Qualidade. São apresentadas na obra as formas de armazenamento das linhagens e as técnicas de caracterização e identificação utilizadas. A Coleção conta com aproximadamente 4500 linhagens, quase todas isoladas de bagas de uvas de diferentes regiões do país, com amplo espectro de ação, e estão à disposição do setor produtivo linhagens com aptidão enológica específica para cada uma das Indicações Geográficas de vinhos brasileiros