“I drive outside of peak time to avoid traffic jams—Public transport is not attractive here.” Challenging discourses on travel to the University campus in Manila

One of the major narratives in transport policy internationally concerns the promotion of private versus public modes. The Global North has many examples where public transport, walking and cycling networks are well developed, yet examples from the Global South are less evident. There is a historical failure of replicating policies and practices from the Global North, particularly in perpetuating the highway building model, often unsuitable to the cultural contexts in the Global South. This paper examines individual attitudes and discourses concerning travel to De La Salle University campus, in Metro Manila, the Philippines. 42 participants are surveyed using Q methodology. Four discourses are developed, reflecting attitudes to growing automobility in Manila, public transport service provision, the difficulties of travelling in the city and the aspiration for increased comfort whilst travelling. Manila provides an example of the complexities in moving towards greater sustainable travel in the southeast Asian context where levels of private car usage are already high. It is hoped that a greater awareness of the problems of the current travel experiences might lead to us to seek different narratives, where transport systems can be developed which better serve social equity and environmental goals

Ground state potential energy surfaces and bound states of M-He dimers (M=Cu,Ag,Au): A theoretical investigation

We present an ab initio investigation on the ground state interaction potentials [potential energy surface (PES)] between helium and the group 11 metal atoms: copper, silver, and gold. To the best of our knowledge, there are no previous theoretical PESs proposed for Cu-He and Au-He, and a single one for Ag-He [Z. J. Jakubek and M. Takami, Chem. Phys. Lett. 265, 653 (1997)], computed about 10 years ago at MP2 level and significantly improved by our study. To reach a high degree of accuracy in the determination of the three M-He potentials (M=Cu,Ag,Au), we performed extensive series of test computations to establish the appropriate basis set, the theoretical method, and the computational scheme for these systems. For each M-He dimer we computed the PES at the CCSD(T) level of theory, starting from the reference unrestricted Hartree-Fock wave function. We described the inner shells with relativistic small core pseudopotentials, and we adopted high quality basis sets for the valence electrons. We also performed CCSDT computations in a limited set of M-He internuclear distances, adopting a medium-sized basis set, such as to define for each dimer a CCSD(T) to CCSDT correction term and to improve further the quality of the CCSD(T) interaction potentials. The Cu-He complex has minimum interaction energy (E(min)) of -28.4 mu hartree at the internuclear distance of 4.59 A (R(min)), and the short-range repulsive wall starts at 4.04 A (R(E=0)). Quite interestingly, the PES of Ag-He is more attractive (E(min)=-33.8 mu hartree) but presents nearly the same R(min) and R(E=0) values, 4.60 and 4.04 A, respectively. The interaction potential for Au-He is markedly deeper and shifted at shorter distances as compared to the lighter complexes, with E(min)=-69.6 mu hartree, R(min)=4.09 A and R(E=0)=3.60 A. As a first insight in the structure of M-He(n) aggregates, we determined the rovibrational structure of the three M-He dimers. The Cu-He and Ag-He potentials support just few rotational excitations, while the Au-He PES admits also a bound vibrational excitation

Condiciones orgánicas y correlaciones de fuerza del movimiento estudiantil chileno.: Una aproximación desde la Confech (2011-2015)*

This paper aims at analyzing the organizational changes in the Confederation of Chilean Students (CONFECH) in the period 2011-2015 and the complexities of the process of institutionalization in the context of the government of Sebastián Piñera and the subsequent administration of Michelle Bachelet. It argues, in the main, that the processes of growth and fragmentation of space CONFECH have generated an increase in the cost of decision-making and collective coordination has become more complex political management capacity of the Executive Board

Multi-payload measurement of transverse velocity shears in the topside ionosphere

Using a multi-payload sounding rocket mission, we present the first direct measurement of velocity shear in the topside auroral ionosphere. In regions of large, ∼200 mV/m, transient electric fields we directly measure differences in the plasma drift velocity. From these differences, shear frequencies reaching ±6Hz are measured. These directly measured shears are compared with the shear inferred from single payload measurements. It is shown this traditional measurement of shear overestimates the shear frequency by a factor of two for this event, highlighting the importance of the temporal component of near-DC electric field structures. Coincident with these strong fields and shears are enhanced emissions of broadband, extremely low frequency (BB-ELF) plasma waves, and a narrowband wave emission near the H+-O+bi-ion resonant frequency

Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide

Combined microbiological test (Biolog Ecoplate, denaturing gradient gel electrophoresis (DGGE) and Real Time PCR (qPCR)) were developed to evaluate the impact of repeated diazinon (DZN) applications at high concentration (40 mg kg-1) on microbial communities in a microcosm simulating the organic matrix (straw (50%): peat (25%): soil (25%) vv-1) of an pesticide biopurification system (PBS). Moreover, pesticide dissipation was also evaluated. After three successive exposition of DZN, dissipation efficiency was high; achieved 87%, 93% and 96% after each application, respectively showing a clear accelerated dissipation of this pesticide in the organic matrix. The results obtained with Biolog Ecoplate showed that community level physiological profiles were no affected by the addition of DZN. On the other hand, molecular assays (DGGE and QPCR) demonstrated that the microbial structure (bacteria and fungi) remained relatively stable over time with high DZN doses compared to control. Therefore, the results of the present study, clearly, demonstrate the high dissipation capacity of this biomixture and highlight the microbiological robustness of this biological system.Fil: Tortella, G. R.. Universidad de la Frontera. Nucleo Cientifico y Tecnologico En Recursos Naturales (bioren-ufro). Departamento de Ciencias Quimicas y Recursos Naturales; ChileFil: Salgado, E.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico En Recursos Naturales; ChileFil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); ArgentinaFil: Mella Herrera, R. A.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico En Recursos Naturales; ChileFil: Parra, L.. Universidad de la Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; ChileFil: Diez, M. C.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico En Recursos Naturales; ChileFil: Rubilar, O.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico En Recursos Naturales; Chil

A New Kind of Quinonic-Antibiotic Useful Against Multidrug-Resistant S. aureus and E. faecium Infections

Indexación: Scopus.A rapid emergence of resistant bacteria is occurring worldwide, endangering the efficacy of antibiotics and reducing the therapeutic arsenal available for treatment of infectious diseases. In the present study, we developed a new class of compounds with antibacterial activity obtained by a simple, two step synthesis and screened the products for in vitro antibacterial activity against ATCC® strains using the broth microdilution method. The compounds exhibited minimum inhibitory concentrations (MIC) of 1⁻32 μg/mL against Gram-positive ATCC® strains. The structure⁻activity relationship indicated that the thiophenol ring is essential for antibacterial activity and the substituents on the thiophenol ring module, for antibacterial activity. The most promising compounds detected by screening were tested against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecium (VREF) clinical isolates. We found remarkable activity against VREF for compounds 7 and 16, were the MIC50/90 were 2/4 µg/mL and 4/4 µg/mL, respectively, while for vancomycin the MIC50/90 was 256/512 µg/mL. Neither compound affected cell viability in any of the mammalian cell lines at any of the concentrations tested. These in vitro data show that compounds 7 and 16 have an interesting potential to be developed as new antibacterial drugs against infections caused by VREF.https://www.mdpi.com/1420-3049/23/7/177

Carbendazim dissipation in the biomixture of on-farm biopurification systems and its effect on microbial communities

The impact of repeated carbendazim (CARB) applications on the extent of \CARB\ dissipation, the microbial diversity, the community level physiological profile (CLPP), and the enzymatic activity within the biomixture of an on-farm biopurification system was evaluated. After three successive \CARB\ applications, the \CARB\ dissipation efficiency was high; the efficiency of dissipation was 87%, 94% and 96% after each application, respectively. Although microbial enzymatic activity was affected significantly by \CARB\ application, it could recover after each \CARB\ pulse. Likewise, the numbers of cultivable bacteria, fungi and actinomycetes (as measured in CFUs) were slightly affected by the addition of CARB, but the inhibitory effect of the pesticide application was temporary. Denaturing gradient gel electrophoresis (DGGE) and Biolog Ecoplate assays demonstrated that the microbial populations remained relatively stable over time when compared to the control. The results obtained herein therefore demonstrate the high dissipation capacity of this biomixture and highlight the microbiological robustness of this biological system.This work was supported by FONDECYT project No 11100236