Creating a Circular Chromosome in the Building Yeast Saccharomyces cerevisiae

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Multi-context Use of Language: Toward Effective Thinking and Planning for Curriculum

The flexible, multi-contextual use of language is essential to integrated learning and thinking. Likewise, learning and thinking in an integrated way is essential to multi-dimensional teaching. This study examines the ways pre-service secondary teachers define their subjects. Students enrolled in a secondary reading methods course were asked to provide multiple definitions for a predetermined list of critical vocabulary terms common to multiple disciplines. We used these definitions to measure participants’ level of sophistication with regard to the multi-context use of language. Participants’ responses illuminated implications for curriculum development in secondary schools including the need for models for pre- and in-service teachers of authentic integrated curriculum

How does innovation emerge in a service ecosystem?

To advance the study of innovation in complex settings, this study integrates the innovation, institutional theory, philosophy, and service-dominant logic literatures. Exploring the emergence of innovation and service ecosystem dynamics, researchers take an abductive approach anchored in over 4 years of case study data regarding a high technology solution in an Internet-of-Things setting. By framing innovation as a systemic process, the study reveals that (1) institutional reconciliation is an overlooked phase of innovation, (2) ideas are refined by four types of institutional reconciliation pressures (tensions, divergences, expected value, and service), and (3) innovation is influenced by plasticity in four ways (recursivity, temporality, complementarity, and continuity). Based on these findings, the authors outline a research agenda regarding four principles of innovation as a systemic process. The findings suggest that managers should nurture norms, rules, and beliefs through a systemic process that facilitates the emergence of innovation

A Novel Vertebral Stabilization Method for Producing Contusive Spinal Cord Injury

Clinically-relevant animal cervical spinal cord injury (SCI) models are essential for developing and testing potential therapies; however, producing reliable cervical SCI is difficult due to lack of satisfactory methods of vertebral stabilization. The conventional method to stabilize the spine is to suspend the rostral and caudal cervical spine via clamps attached to cervical spinous processes. However, this method of stabilization fails to prevent tissue yielding during the contusion as the cervical spinal processes are too short to be effectively secured by the clamps (Figure 1). Here we introduce a new method to completely stabilize the cervical vertebra at the same level of the impact injury. This method effectively minimizes movement of the spinal column at the site of impact, which greatly improves the production of consistent SCIs. We provide visual description of the equipment (Figure 2-4), methods, and a step-by-step protocol for the stabilization of the cervical 5 vertebra (C5) of adult rats, to perform laminectomy (Figure 5) and produce a contusive SCI thereafter. Although we only demonstrate a cervical hemi-contusion using the NYU/MASCIS impactor device, this vertebral stabilization technique can be applied to other regions of the spinal cord, or be adapted to other SCI devices. Improving spinal cord exposure and fixation through vertebral stabilization may be valuable for producing consistent and reliable injuries to the spinal cord. This vertebral stabilization method can also be used for stereotactic injections of cells and tracers, and for imaging using two-photon microscopy in various neurobiological studies

Perceptions, Barriers, and Suggestions for Creation of a Tobacco and Health Website among American Indian/Alaska Native College Students

Information concerning American Indian/Alaska Native (AI/AN) Internet use and health information needs is dearth. Our research team explored Internet use among AI/AN college students to determine Internet use in relation to health information seeking behaviors. We used a tobacco site example for participants to describe what they desired in a health site designed specifically for AI/AN. Using a community-based participatory research approach, we conducted 14 focus groups with AI/AN college students (N=108), to better understand their perceptions of and attitudes toward Internet use and health information needs. Daily Internet use was reported across strata yet health topics investigated differed among groups. Participants in all strata desired a health website that was easy to navigate and interactive. Respectful representation of Native culture was a concern, yet no consensus was reached for a multi-tribal audience. Participants felt a website should use caution with cultural depictions due to the possible misinterpretation. Overall, participants agreed that recreational and traditional tobacco use should be differentiated and the variation of traditional use among tribes acknowledged. Data concerning Internet use for health information among AI/AN college students are needed to establish baseline indicators to effectively address disparities

Spenders and Tightwads Among Newlyweds: Perceptions of Partner Financial Behaviors and Relational Well-Being

Finances, and how couples manage their finances, can have important implications for couples’ relational well-being. Using data from 1,585 couples that participated in the CREATE study (a nationally representative dyadic dataset of U.S. newlywed couples), we examined how perceiving one’s spouse as a financial spender (i.e., spending more than they ideally would) or financial tightwad (i.e., spending less than they ideally would) was associated with several measures of relational well-being (i.e., satisfaction, commitment, and power) through actor-partner interdependence structural equation models. Results showed that perceiving one’s partner as a spender was detrimental for both the individual’s and the partner’s marital satisfaction, marital commitment, and marital power. Perceiving one’s partner as a tightwad was detrimental for both the individual’s and the partner’s marital commitment and marital power. The findings suggest that interventions focused on perceptions of financial management behaviors may help strengthen relational well-being among newlyweds

American Indian/Alaska Native Willingness to Provide Biological Samples for Research Purpose

This article examines the willingness of American Indian/Alaska Natives (AI/AN) to provide biological samples for research purposes. Prior cases of abuse and misuse of individuals, materials, and data highlight ethical research concerns. Investigators may be hesitant to engage AI/ANs in research projects. We conducted a survey of AI/ANs in the central plains region of the US over 1 year. This convenience sample completed a series of questions on biological samples and research. Survey results (N = 998) indicate that 70.15% of AI/ANs would be willing to provide saliva/spit for a specific study with the proper consent and control of samples. In conclusion, researchers should find ways to work with and for AI/ANs, assuring participant input in the research process

Disk and Envelope Structure in Class 0 Protostars: I. The Resolved Massive Disk in Serpens FIRS 1

We present the first results of a program to characterize the disk and envelope structure of typical Class 0 protostars in nearby low-mass star forming regions. We use Spitzer IRS mid-infrared spectra, high resolution CARMA 230 GHz continuum imaging, and 2-D radiative transfer models to constrain the envelope structure, as well as the size and mass of the circum-protostellar disk in Serpens FIRS 1. The primary envelope parameters (centrifugal radius, outer radius, outflow opening angle, and inclination) are well constrained by the spectral energy distribution (SED), including Spitzer IRAC and MIPS photometry, IRS spectra, and 1.1 mm Bolocam photometry. These together with the excellent uv-coverage (4.5-500 klam) of multiple antenna configurations with CARMA allow for a robust separation of the envelope and a resolved disk. The SED of Serpens FIRS 1 is best fit by an envelope with the density profile of a rotating, collapsing spheroid with an inner (centrifugal) radius of approximately 600 AU, and the millimeter data by a large resolved disk with Mdisk~1.0 Msun and Rdisk~300 AU. These results suggest that large, massive disks can be present early in the main accretion phase. Results for the larger, unbiased sample of Class~0 sources in the Perseus, Serpens, and Ophiuchus molecular clouds are needed to determine if relatively massive disks are typical in the Class 0 stage.Comment: Comments: 13 pages, 8 figures, 3 tables; accepted for publication in the Ap

Data Management Strategy to Improve Global Use of Ocean Acidification Data and Information

International audienceOcean acidification (OA) refers to the general decrease in pH of the global ocean as a result of absorbing anthropogenic CO2 emitted in the atmosphere since preindustrial times (Sabine et al., 2004). There is, however, considerable variability in ocean acidification, and many careful measurements need to be made and compared in order to obtain scientifically valid information for the assessment of patterns, trends, and impacts over a range of spatial and temporal scales, and to understand the processes involved. A single country or institution cannot undertake measurements of worldwide coastal and open ocean OA changes; therefore, international cooperation is needed to achieve that goal. The OA data that have been, and are being, collected represent a significant public investment. To this end, it is critically important that researchers (and others) around the world are easily able to find and use reliable OA information that range from observing data (from time-series moorings, process studies, and research cruises), to biological response experiments (e.g., mesocosm), data products, and model output. [...

Functional and Histological Gender Comparison of Age-Matched Rats after Moderate Thoracic Contusive Spinal Cord Injury

Spinal cord injury (SCI) afflicts hundreds of thousands of Americans, and most SCI (∼80%) occurs in males. In experimental animal models, however, many studies used females. Funding agencies like the National Institutes of Health recommend that new proposed studies should include both genders due to variations in gender response to injuries, diseases, and treatments. However, cost and considerations for some animal models, such as SCI, affect investigators in adapting to this recommendation. Research has increased comparing gender effects in various disease and injury models, including SCI. However, most studies use weight-matched animals, which poses issues in comparing results and outcomes. The present study compared histologic and functional outcomes between age-matched male and female Sprague-Dawley rats in a moderate thoracic contusion SCI model. Cresyl violet and eosin staining showed no significant differences in lesion volume between genders after 9 weeks post-SCI (p > 0.05). Luxol fast blue–stained spared myelin was similar between genders, although slightly greater (∼6%) in spared myelin, compared with cord volume (p = 0.044). Glial reactivity and macrophage labeling in the lesion area was comparable between genders, as well. Basso, Beattie, Bresnahan (BBB) functional scores were not significantly different between genders, and Hargreaves thermal hyperalgesia and Gridwalk sensorimotor analyses also were similar between genders, compared with uninjured gender controls. Analysis of covariance showed weight did not influence functional recovery as assessed through BBB (p = 0.65) or Gridwalk assessment (p = 0.63) in this study. In conclusion, our findings suggest age-matched male and female rats recover similarly in a common clinically relevant SCI model