Masses and decay constants of Bc(∗)B_c^{(*)} mesons with Nf=2+1+1N_f=2+1+1 twisted mass fermions

We present a preliminary lattice determination of the masses and decay constants of the pseudoscalar and vector mesons $B_c$ and $B_c^*$. Our analysis is based on the gauge configurations produced by the European Twisted Mass Collaboration with $N_f = 2 + 1 + 1$ flavors of dynamical quarks. We simulated at three different values of the lattice spacing and with pion masses as small as 210 MeV. Heavy-quark masses are simulated directly on the lattice up to $\sim 3$ times the physical charm mass. The physical b-quark mass is reached using the ETMC ratio method. Our preliminary results are: $M_{B_c} = 6341\,(60)$ MeV, $f_{B_c} = 396\,(12)$ MeV, $M_{B_c^*} / M_{B_c} = 1.0037\,(39)$ and $f_{B_c^*} / f_{B_c} = 0.987\,(7)$.Comment: 7 pages, 3 figures, 1 table; contribution to the proceedings of the XXXVI Int'l Workshop on Lattice Field Theory (LATTICE2018), July 22-28, 2018, East Lansing, Michigan State University (Michigan, USA

Drug-induced disseminated intravascular coagulation: a pharmacovigilance study on World Health Organization’s database

Background: Disseminated intravascular coagulation (DIC) occurs in several clinical conditions, including drug therapy. We aim to investigate the association between the administration of several drug classes and the onset of DIC by using the reports of Adverse Drug Reactions (ADR) collected in Vigibase, the World Health Organization (WHO) database of ADR. Methods: We collected reports of drug-related DIC from 1968 to September 2015, classified in Vigibase according to the MedDRA (Medical Dictionary for Regulatory Activities) term “Disseminated intravascular coagulation”. A disproportionality analysis using Reporting Odds Ratio (ROR) with 95% Confidence Interval (CI95%) was performed. Results: Overall, 4653 reports of drug-associated DIC were retrieved and the 75.9% of them was serious according to WHO seriousness criteria. DIC was significantly (ROR > 1, lower limit of CI95% > 1) associated with 88 drugs, mainly antineoplastic agents, antithrombotic agents and antibacterials for systemic use. Among of the most frequently reported individual drugs we found dabigatran (94 reports) ROR = 1.34 (CI95% 1.08–1.67), oxaliplatin and bevacizumab both with 75 reports and ROR = 1.77 (1.38–2.27) and 2.02 (1.57–2.61), respectively. Conclusion: A substantial number of drugs, widely used in the clinical practice, may be associated with the potential occurrence of DIC. For many of these drugs, the ADR is not acknowledged in the corresponding Summary of Product Characteristics. The high number of drugs involved underlines the importance of evaluate this condition such as an ADR that might occur during drug therapy

Masses and decay constants of Bc(∗)B_c^{(*)} mesons with Nf=2+1+1N_f=2+1+1 twisted mass fermions

We present a preliminary lattice determination of the masses and decay constants of the pseudoscalar and vector mesons $B_c$ and $B_c^*$. Our analysis is based on the gauge configurations produced by the European Twisted Mass Collaboration with $N_f = 2 + 1 + 1$ flavors of dynamical quarks. We simulated at three different values of the lattice spacing and with pion masses as small as 210 MeV. Heavy-quark masses are simulated directly on the lattice up to $\sim 3$ times the physical charm mass. The physical b-quark mass is reached using the ETMC ratio method. Our preliminary results are: $M_{B_c} = 6341\,(60)$ MeV, $f_{B_c} = 396\,(12)$ MeV, $M_{B_c^*} / M_{B_c} = 1.0037\,(39)$ and $f_{B_c^*} / f_{B_c} = 0.987\,(7)$.Comment: 7 pages, 3 figures, 1 table; contribution to the proceedings of the XXXVI Int'l Workshop on Lattice Field Theory (LATTICE2018), July 22-28, 2018, East Lansing, Michigan State University (Michigan, USA

Impact of Horse Grazing on Floristic Diversity in Mediterranean Small Standing-Water Ecosystems (SWEs)

Small standing-Water Ecosystems (SWEs), despite their pivotal ecological role due to their participation in hydrogeological processes and their richness in biodiversity, seem to be often overlooked by the scientific community. In this study, the vascular plant diversity in some representative SWEs, that host a peculiar assemblage of plant and animal species, was investigated in relation to the disturbance effects of a wild horse population. A total of 50 plots, equally distributed in small and large SWEs, were surveyed and a level of disturbance was attributed to each plot. We found greater species richness in small and undisturbed SWEs, which suggests the negative impact of horse grazing on the richness of plant species in this type of habitat. Significant differences in plant assemblage were found according to the disturbance level, whereas, contrary to what was observed for species richness, no differences were detected based on their size. The diversity indices, used to evaluate the richness and diversity in these areas, recorded the highest values for small and undisturbed areas. This result highlights that the disturbance of the horse grazing plays a pivotal role in affecting the diversity and richness of species in the SWEs. These findings suggest that SWE systems should be analyzed considering these areas as unique in order to allow the conservation of the plant richness and biodiversity of the SWE systems in conjunction with the protection of horses

Towards the Creation of Interdisciplinary Consumer-Oriented Security Metrics

Information systems are evolving: IoT devices and Cyber-physical systems (CPS) impact on the security of assets and people in the real world. Old cybersecurity approaches, which focused on seeing humans 'as a problem', could be substitute by new paradigms of seeing humans 'as a solution'. Therefore, consumers awareness will be one of the building blocks, as well as initiative that aim to create a set of standardized security metrics that can evaluate the security of systems. In order to do that, researchers need to study which are the essential factors that our future metrics should focus on. In this paper we analyzed this problem over CPS while assuming the consumer perspective. We summarize the state of the art in security metrics and advocate the need for a research effort aimed at taking the field to a new level of formal soundness and practical usability by considering interdisciplinary implications on cybersecurity

Crohn's-like ileo-colitis in patients affected by glycogen storage disease Ib: Two years' follow-up of patients with a wide spectrum of gastrointestinal signs

Aim: To investigate the presence of inflammatory bowel disease (IBD) and to evaluate the progression of bowel involvement after two years' follow-up in seven patients affected by glycogen storage disease type Ib (GSDIb). Methods: Seven patients (5F, 2M, aged 4.5-20.6 y) entered the study. Bowel involvement was evaluated by ileocolonoscopy and specific IBD serologic markers. To evaluate disease activity, Paediatric Crohn's Disease Activity Index (PCDAI), terminal ileum wall thickness detected at ultrasonography (US), 99mTechnetium labelled autologous White Cell Scan (Tc-WCS) and barium meal with follow-through were investigated. Results: Ileocolonoscopy and histology examination revealed variable degrees of bowel involvement in all patients. The results of serologic markers were indicative of a Crohn's-like ileocolitis. US and Tc-WCS, could clearly define patients with severe inflammatory involvement, but failed to identify all patients with mild to moderate disease. For the most severely affected patients, anti-inflammatory agents and steroids were prescribed, whereas nutritional therapy with polymeric formula and antibiotics were assumed by two other patients and antibiotics only by one patient. Granulocyte colony-stimulating factor (G-CSF) was prescribed to all patients. Ileocolonoscopy and histology data improved in all patients. The assumption of G-CSF and/or gastric drip feeding (g.d.f.) was inversely associated with the PCDAI results (p < 0.05). Conclusion: IBD is common in patients affected by GSDIb independently of the severity of gastrointestinal signs and symptoms. Different therapeutic approaches can be used according to the severity of IBD. G-CSF treatment and g.d.f. can be protective factors for IBD

Liver proteome dataset of Sparus aurata exposed to low temperatures

We report the proteomic dataset of livers from Sparus aurata exposed to low temperature during growth. Gilthead sea bream juveniles were reared in Recirculating Aquaculture Systems (RAS) and exposed to a temperature ramp made of two phases of four weeks each: a Cooling phase from 18&nbsp;\ub0C (t0) to 11&nbsp;\ub0C (t1) and a Cold Maintenance phase at 11&nbsp;\ub0C (t1-t2) in a 8 week feeding trial. At the end of the experiment, sea bream livers were collected and analyzed with a shotgun proteomics approach based on filter-aided sample preparation followed by tandem mass spectrometry, peptide identification carried out using Sequest-HT as search engine within the Proteome Discoverer informatic platform, and label-free differential analysis. The mass spectrometry data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD011059 (Vizca\uedno et al., 2016; Deutsch et al., 2017; Perez-Riverol et al., 2016). The dataset described here is also related to the research article entitled "Liver proteomics of gilthead sea bream (Sparus aurata) exposed to cold stress" (Ghisaura et al., 2019)

Liver proteomics of gilthead sea bream (Sparus aurata) exposed to cold stress

The gilthead sea bream (Sparus aurata, L.) is very sensitive to low temperatures, which induce fasting and reduced growth performances. There is a strong interest in understanding the impact of cold on fish metabolism to foster the development and optimization of specific aquaculture practices for the winter period. In this study, an 8 week feeding trial was carried out on gilthead sea bream juveniles reared in a Recirculated Aquaculture System (RAS) by applying a temperature ramp in two phases of four weeks each: a cooling phase from 18 \ub0C to 11 \ub0C and a cold maintenance phase at 11 \ub0C. Liver protein profiles were evaluated with a shotgun proteomics workflow based on filter-aided sample preparation (FASP) and liquid chromatography-mass spectrometry (LC-ESI-Q-TOF MS/MS) followed by label-free differential analysis. Along the whole trial, sea breams underwent several changes in liver protein abundance. These occurred mostly during the cooling phase when catabolic processes were mainly observed, including protein and lipid degradation, together with a reduction in protein synthesis and amino acid metabolism. A decrease in protein mediators of oxidative stress protection was also seen. Liver protein profiles changed less during cold maintenance, but pathways such as the methionine cycle and sugar metabolism were significantly affected. These results provide novel insights on the dynamics and extent of the metabolic shift occurring in sea bream liver with decreasing water temperature, supporting future studies on temperature-adapted feed formulations. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD011059

Viral expression and molecular profiling in liver tissue versus microdissected hepatocytes in hepatitis B virus - associated hepatocellular carcinoma.

Background: The molecular mechanisms whereby hepatitis B virus (HBV) induces hepatocellular carcinoma (HCC) remain elusive. We used genomic and molecular techniques to investigate host-virus interactions by studying multiple areas of the same liver from patients with HCC. Methods: We compared the gene signature of whole liver tissue (WLT) versus laser capture-microdissected (LCM) hepatocytes along with the intrahepatic expression of HBV. Gene expression profiling was performed on up to 17 WLT specimens obtained at various distances from the tumor center from individual livers of 11 patients with HCC and on selected LCM samples. HBV markers in liver and serum were determined by real-time polymerase chain reaction (PCR)and confocal immunofluorescence. Results: Analysis of 5 areas of the liver showed a sharp change in gene expression between the immediate perilesional area and tumor periphery that correlated with a significant decrease in the intrahepatic expression of HB surface antigen (HBsAg). The tumor was characterized by a large preponderance of down-regulated genes, mostly involved in the metabolism of lipids and fatty acids, glucose, amino acids and drugs, with down-regulation of pathways involved in the activation of PXR/RXR and PPARα/RXRα nuclear receptors, comprising PGC-1α and FOXO1, two key regulators critically involved not only in the metabolic functions of the liver but also in the life cycle of HBV, acting as essential transcription factors for viral gene expression. These findings were confirmed by gene expression of microdissected hepatocytes. Moreover, LCM of malignant hepatocytes also revealed up-regulation of unique genes associated with cancer and signaling Pathways, including two novel HCC-associated cancer testis antigen genes, NUF2 and TTK. Conclusions: Integrated gene expression profiling of whole liver tissue with that of microdissected hepatocytes demonstrated that HBV-associated HCC is characterized by a metabolism switch-off and by a significant reduction in HBsAg. LCM proved to be a critical tool to validate gene signatures associated with HCC and to identify genes that may play a role in hepatocarcinogenesis, opening new perspectives for the discovery of novel diagnostic markers and therapeutic targets