Ionization vacuum gauge with all but the end of the ion collector shielded Patent

Describing hot filament type Bayard-Alpert ionization gage with ion collector buried or removed from grid structur

Nonlinear renewal theory for Markov random walks

AbstractLet {Sn} be a Markov random walk satisfying the conditions of Kesten's Markov renewal theorem. It is shown that if {Zn} is a stochastic process whose finite-dimensional, conditional distributions are asymptotically close to those of {Sn} (in the sense of weak convergence), then the overshoot of {Zn} has the same limiting distribution as that of {Sn}. In the case where {Zn} can be represented as a perturbed Markov random walk, this allows substantial weakening of the slow change condition on the perturbation process; more importantly, no such representation is required. An application to machine breakdown times is given

Primitive abundant and weird numbers with many prime factors

We give an algorithm to enumerate all primitive abundant numbers (briefly, PANs) with a fixed $\Omega$ (the number of prime factors counted with their multiplicity), and explicitly find all PANs up to $\Omega=6$, count all PANs and square-free PANs up to $\Omega=7$ and count all odd PANs and odd square-free PANs up to $\Omega=8$. We find primitive weird numbers (briefly, PWNs) with up to 16 prime factors, improving the previous results of [Amato-Hasler-Melfi-Parton] where PWNs with up to 6 prime factors have been given. The largest PWN we find has 14712 digits: as far as we know, this is the largest example existing, the previous one being 5328 digits long [Melfi]. We find hundreds of PWNs with exactly one square odd prime factor: as far as we know, only five were known before. We find all PWNs with at least one odd prime factor with multiplicity greater than one and $\Omega = 7$ and prove that there are none with $\Omega < 7$. Regarding PWNs with a cubic (or higher) odd prime factor, we prove that there are none with $\Omega\le 7$, and we did not find any with larger $\Omega$. Finally, we find several PWNs with 2 square odd prime factors, and one with 3 square odd prime factors. These are the first such examples.Comment: New section on open problems. A mistake in table 2 corrected (# odd PAN with Omega=8). New PWN in table 5, last line, 2 squared prime factors, Omega=15. Updated bibliograph

A new approach to mass spectrometer measurements of thermospheric density

The gas sampling problem in satellite and high velocity probes was investigated by applying the theory of a drifting Maxwellian gas. A lens system using a free stream ion source was developed and experimentally evaluated over the pressure range of 0.00001 to 0.01 N/m sq (approx. 10 to the minus 7th power to 0.0001 torr). The source has high beam transparency, which minimizes gas-surface collisions within, or near, the ionization volume. It is shown that for high ion energy (60 eV), the extracted ion beam has an on-axis energy spread of less than 4 eV, and that 90 percent of the ions are within 2.5 deg of the beam axis. It is concluded that the molecular beam mass spectrometer concept, developed for gas density measurements in the upper atmosphere, substantially reduces gas-surface scattering and gas-surface reactions in the sample, and preserves the integrity of the gas sample during the analysis process. Studies show that both the Scout and Delta launch vehicles have adequate volume, control, velocity, and data acquisition capability necessary to obtain thermospheric number density in real time

M2 muscarinic receptor activation inhibits cell proliferation and migration of rat adipose-mesenchymal stem cells

Mesenchymal stem cells (MSCs), also known as stromal mesenchymal stem cells, are multipotent cells, which can be found in many tissues and organs as bone marrow, adipose tissue and other tissues. In particular MSCs derived from Adipose tissue (ADSCs) are the most frequently used in regenerative medicine because they are easy to source, rapidly expandable in culture and excellent differentiation potential into adipocytes, chondrocytes and other cell types. Acetylcholine (ACh), is one of the most important neurotransmitter in central (CNS) and peripheral nervous system (PNS), playing important roles also in non-neural tissue, but its functions in MSCs are still not investigated. Although MSCs express muscarinic receptor subtypes, their role is completely unknown. In present work muscarinic cholinergic effects were characterized in rat ADSCs. Analysis by RT-PCR demonstrates that ADSCs express M1-M4 muscarinic receptor subtypes, whereas M2 is one of the most expressed subtype. For this reason, our attention was focused on M2 subtype. By using the selective M2 agonist Arecaidine Propargyl Ester (APE) we performed cell proliferation and migration assays demonstrating that APE causes cell growth and migration inhibition without affecting cell survival. Our results indicate that ACh via M2 receptors, may contribute to the maintaining of the ADSCs quiescent status. These data are the first evidence that ACh via muscarinic receptors might contribute to control ADSCs physiology

P2X7 mRNA expression in non-small cell lung cancer: MicroRNA regulation and prognostic value

The human P2X7 receptor is significant and exhibits several functions in neoplasia. At present, little is known with regard to its regulation. P2X7 expression may be regulated post-transcriptionally and putative microRNA (miRNA) binding sites are considered to be involved. The aim of this study was to determine whether miRNAs (miR-21, let-7 g and miR-205) regulate P2X7 mRNA stability. In addition, the impact of P2X7 expression in patients with non-small cell lung cancer (NSCLC) was investigated. P2X7 mRNA and mature Let-7 g, miR-21, and miR-205 expression levels were quantified in 96 NSCLC cases using quantitative reverse transcription polymerase chain reaction. In all samples, epidermal growth factor receptor and K-Ras mutational analysis was also performed. Samples with low P2X7 expression were found to exhibit a higher fold change in miR-21 expression when compared with samples exhibiting high P2X7 expression. Significantly higher miR-21 expression was observed in the tumors of NSCLC patients with a K-Ras mutation when compared with patients who had K-Ras wild-type tumors (P=0.003). Additionally, to evaluate the association between P2X7 expression and prognosis in NSCLC patients, survival analysis was performed using the Kaplan-Meier method. A significant difference in the progression-free survival and overall survival in the NSCLC patients with high P2X7 expression was identified, when compared with that of patients with low expression (P=0.03 and P=0.02, respetively). Therefore, we hypothesized that high levels of miR-21 expression in NSCLC patients with K-Ras mutations may be regulated by a complex circuit, including P2X7 downregulation and together these processes may promote tumor progression

Site-Specific RNA Editing of Stop Mutations in the CFTR mRNA of Human Bronchial Cultured Cells

It is reported that about 10% of cystic fibrosis (CF) patients worldwide have nonsense (stop) mutations in the CFTR gene, which cause the premature termination of CFTR protein synthesis, leading to a truncated and non-functional protein. To address this issue, we investigated the possibility of rescuing the CFTR nonsense mutation (UGA) by sequence-specific RNA editing in CFTR mutant CFF-16HBEge, W1282X, and G542X human bronchial cells. We used two different base editor tools that take advantage of ADAR enzymes (adenosine deaminase acting on RNA) to edit adenosine to inosine (A-to-I) within the mRNA: the REPAIRv2 (RNA Editing for Programmable A to I Replacement, version 2) and the minixABE (A to I Base Editor). Immunofluorescence experiments show that both approaches were able to recover the CFTR protein in the CFTR mutant cells. In addition, RT-qPCR confirmed the rescue of the CFTR full transcript. These findings suggest that site-specific RNA editing may efficiently correct the UGA premature stop codon in the CFTR transcript in CFF-16HBEge, W1282X, and G542X cells. Thus, this approach, which is safer than acting directly on the mutated DNA, opens up new therapeutic possibilities for CF patients with nonsense mutations

Phylogenetic relationships of the italian populations of horseshoe whip snake hemorrhois hippocrepis (Serpentes, colubridae)

Hemorrhois hippocrepis is a colubrid snake with a West Mediterranean distribution. It is widespread in the Iberian Peninsula and Northwest Africa. The only Italian populations are found on the islands of Sardinia and Pantel-leria. The phylogenetic relationships of these insular populations have been analysed for the first time on the basis of the mitochondrial DNA cytochrome b gene. The sequences were compared with those available from the geographic range of this species. The analyses showed that the Italian samples are part of a lineage that groups Tunisian and East Algerian samples, with which they share the same haplotype. These results strongly support the hypothesis of a recent origin of the Italian populations of Hemorrhois hippocrepis, probably determined by human-mediated dispersal from North Africa