268 research outputs found
Validation of a self-reported work disability questionnaire for ulcerative colitis
Ulcerative colitis (UC) may severely limit patients'' capacity to work. Recently, we validated a work disability questionnaire (WDQ) for Crohn disease. As UC shares clinical characteristics with Crohn disease, we hypothesized that the questionnaire might also be useful for UC. The study was aimed to validate the WDQ for use in UC. Consecutive patients with UC (n = 142, 67 women; age 48 +/- 1) completed the UC-WDQ and the inflammatory bowel disease questionnaire-9 (IBDQ-9), and EuroQoL-5D quality-of-life questionnaires. Validation of the UC-WDQ included an assessment of its construct validity, including: discriminant validity, convergent validity, and reproducibility (test-retest). We also calculated the intraclass correlation and the Cronbach alpha. 1. Discriminant validity: Mean UC-WDQ scores were 12.8 +/- 4.4 (remission phase) and 17.2 +/- 6.1 (with clinical activity) (P < .05). 2. Convergent validity: The correlations of UC-WDQ were r = 0.74 (P < .001) with IBDQ, r = 0.44 (P < .01) with disease activity, r = 0.56 (P < .01) with EuroQoL-5D, and r = 0.60 (P < .01) with the EuroQoL-5D visual scale. 3. Reproducibility: Test-retest reproducibility: UC-WDQ scores obtained after a 2-week interval were similar (15.8 vs 15.1), (r = 0.91, P < .01). Intraclass correlation was 0.93 (95% confidence interval 0.92-0.95). Cronbach alpha was 0.94. The UC-WDQ is a valid and reliable tool for measuring work disability in patients with UC
Inferior Fronto-Occipital fascicle anatomy in brain tumor surgeries: From anatomy lab to surgical theater
DIFFERENT AQUAPORIN-4 EXPRESSION IN GLIOBLASTOMA MULTIFORME PATIENTS WITH AND WITHOUT SEIZURES
Aquaporin-4 (AQP-4), the most important water channel in the brain, is expressed by astrocyte endfeet abutting microvessels. Altered expression levels of AQP-4 and redistribution of the protein throughout the membranes of cells found in glioblastoma multiforme (GBM) lead to development of the oedema often found surrounding the tumour mass. Dysregulation of AQP-4 also occurs in hippocampal sclerosis and cortical dysplasia in patients with refractory partial epilepsy. This work reports on analysis of the relationship between AQP-4 expression and the incidence of epileptic seizures in patients with GBM. Immunohistochemical and PCR techniques were used to evaluate AQP-4 in biopsy specimens from 19 patients with GBM, 10 of whom had a history of seizures prior to surgery. AQP-4 mRNA levels were identical in the two groups of patients, but AQP-4 expression was more frequently detected on the GBM membranes from specimens of patients with seizures than from those without (10 vs. 2,
The Targeted Therapies Era Beyond the Surgical Point of View: What Spine Surgeons Should Know Before Approaching Spinal Metastases
PGI12 Organizational and Economic Issues Related to the Introduction of Boceprevir in the Treatment of Patients with Genotype 1 Chronic Hepatitis C in Italy
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Let me see: Correlation between 5-ala fluorescence and molecular pathways in glioblastoma: A single center experience
Background: Despite the aggressiveness of multimodal treatment, glioblastoma (GBM) is still a challenge for neurosurgeons, neurooncologists, and radiotherapists. A surgical approach is still a cornerstone in GBM therapeutic management, as the extent of resection is strongly related both to overall survival and progression-free survival. From this perspective, the use of photodynamic molecules could represent an interesting tool to achieve maximal and safe resection. Being able to trace the lesion’s edges, indeed, could allow to improve the extent of resection and to minimize residual tumor while sparing normal tissue. The use of 5-aminolevulinic acid (5-ALA) as a photodynamic tracer is well established due to its strict correlation both with cellularity and metabolic activity of the GBM cell clones. Objective: Our study aims to define whether a different molecular asset of GBM (especially investigating IDH 1/2 mutation, proliferation index, and MGMT promoter methylation) results in different fluorescence expression, possibly because of differences in metabolic pathways due to different genotypes. Methods: Patients undergoing surgery for GBM removal at our Institute (Dep. Of Neurosurgery, Ospedale Città della Salute e della Scienza, University of Turin, Italy) were retrospectively reviewed. Patients with histological diagnosis confirmation and to whom 5-ALA was given before surgery were included. The whole surgical procedure was recorded and then analyzed by three different people (a medical student, a resident, and a senior surgeon with an interest in neurooncology and experience in using 5-ALA) and a score was assigned to the different degrees of intraoperative fluorescence. The degree of fluorescence was then matched with the genotype. Results: A trend of grade 2 fluorescence (i.e., ”strong”) was observed in the IDH 1/2 wild-type (WT) genotype, suggesting a more intense metabolic activity in this particular subgroup, while, no or weak fluorescence was observed more often in the IDH 1/2 mutated tumors, suggesting a lower metabolic activity. No relations were found between fluorescence grade and MGMT promoter methylation or, interestingly, cellularity. As a secondary analysis, more epileptogenicity of the IDH 1/2 mutated GBM was noticed, similarly to other recent literature. Conclusion: Our results do not support the use of 5-ALA as a diagnostic tool, or a way to substitute the molecular profiling, but confirm 5-ALA as a powerful metabolic tracer, able to easily detect the pathological cells, especially in the IDH WT genotype, and in this perspective, further studies will be necessary to better describe the metabolic activity of GBM cells
Proton-air cross section measurement with the ARGO-YBJ cosmic ray experiment
The proton-air cross section in the energy range 1-100 TeV has been measured
by the ARGO-YBJ cosmic ray experiment. The analysis is based on the flux
attenuation for different atmospheric depths (i.e. zenith angles) and exploits
the detector capabilities of selecting the shower development stage by means of
hit multiplicity, density and lateral profile measurements at ground. The
effects of shower fluctuations, the contribution of heavier primaries and the
uncertainties of the hadronic interaction models, have been taken into account.
The results have been used to estimate the total proton-proton cross section at
center of mass energies between 70 and 500 GeV, where no accelerator data are
currently available.Comment: 14 pages, 9 figure
Gamma ray flares from Mrk421 in 2008 observed with the ARGO-YBJ detector
In 2008 the blazar Markarian 421 entered a very active phase and was one of
the brightest sources in the sky at TeV energies, showing frequent flaring
episodes. Using the data of ARGO-YBJ, a full coverage air shower detector
located at Yangbajing (4300 m a.s.l., Tibet, China), we monitored the source at
gamma ray energies E > 0.3 TeV during the whole year. The observed flux was
variable, with the strongest flares in March and June, in correlation with
X-ray enhanced activity. While during specific episodes the TeV flux could be
several times larger than the Crab Nebula one, the average emission from day 41
to 180 was almost twice the Crab level, with an integral flux of (3.6 +-0.6)
10^-11 photons cm^-2 s^-1 for energies E > 1 TeV, and decreased afterwards.
This paper concentrates on the flares occurred in the first half of June.
This period has been deeply studied from optical to 100 MeV gamma rays, and
partially up to TeV energies, since the moonlight hampered the Cherenkov
telescope observations during the most intense part of the emission. Our data
complete these observations, with the detection of a signal with a statistical
significance of 3.8 standard deviations on June 11-13, corresponding to a gamma
ray flux about 6 times larger than the Crab one above 1 TeV. The reconstructed
differential spectrum, corrected for the intergalactic absorption, can be
represented by a power law with an index alpha = -2.1 extending up to several
TeV. The spectrum slope is fully consistent with previous observations
reporting a correlation between the flux and the spectral index, suggesting
that this property is maintained in different epochs and characterizes the
source emission processes.Comment: Accepted for publication on ApJ
The ARGO-YBJ Experiment Progresses and Future Extension
Gamma ray source detection above 30TeV is an encouraging approach for finding
galactic cosmic ray origins. All sky survey for gamma ray sources using wide
field of view detector is essential for population accumulation for various
types of sources above 100GeV. To target the goals, the ARGO-YBJ experiment has
been established. Significant progresses have been made in the experiment. A
large air shower detector array in an area of 1km2 is proposed to boost the
sensitivity. Hybrid detection with multi-techniques will allow a good
discrimination between different types of primary particles, including photons
and protons, thus enable an energy spectrum measurement for individual specie.
Fluorescence light detector array will extend the spectrum measurement above
100PeV where the second knee is located. An energy scale determined by balloon
experiments at 10TeV will be propagated to ultra high energy cosmic ray
experiments
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