Maternal and Congenital cytomegalovirus infection and zero rubella IgM prevalence in newborns in St.Paul’s Hospital Millennium Medical College

Additional file 1. Questioners

Seroepidemiology study of cytomegalovirus and rubella among pregnant women at St. Paul’s Hospital Millennium Medical College, Addis Ababa, Ethiopia

Background: Maternal cytomegalovirus (CMV) and rubella infections have adverse neonatal outcomes. Both CMV and rubella are more widespread in developing countries and in communities with lower socioeconomic status. The aim of this study was to investigate sero-prevalence of CMV and rubella infection and associated possible risk factors.Method: Using cross sectional study design a total of 200 pregnant women were consecutively recruited starting from June and July 2014. Blood samples were collected, and structured questions were used to gather socio-demographic and risk factor related data. ELISA was used to detect CMV (IgG, IgM) and rubella IgM. SPSS version 20 was used to analyze the data, and regression was also performed.Results: Out of 200 pregnant women, 88.5%, 30(15.5%) and 4(2.0%) were CMV-IgG, CMV- IgM, and rubella-IgM positive, respectively. Women who were immune/positive only for IgG were 73.5%. The second group was those with primary infection [IgG (+) plus IgM (+)] and this consisted of 15.0% participants. Eleven percent of the participants were at high risk for primary infection during their pregnancy. One pregnant woman was identified as having a recent primary infection. In this study, no statistically significant association was detected between CMV infection with idependent factors (p-value>0.05).Conclusion: In addition to detection of high prevalence of CMV, detecting recent infection of rubella worsens the outcome of the disease. Rubella vaccine should be taken into consideration after large scale surveillance. However, screening of all pregnant women for CMV infection may not be cost-effective as in the countries with high seropositivity.Keywords: Cytomegalovirus, Human cytomegalovirus, Rubella, Seroprevalence, Pregnanc

Epidemiology of rubella virus cases in the pre-vaccination era of Ethiopia, 2009–2015

Abstract Background Rubella is a common mild rash illness caused by rubella virus. The majority of infections occur in children and young adults. The infection is the cause of a serious birth defect known as Congenital Rubella Syndrome (CRS) when a woman acquires infection early in pregnancy. Ethiopia has not yet established rubella virus surveillance and has not yet introduced rubella vaccine into the routine immunization program. We characterize the epidemiology of laboratory confirmed rubella virus cases collected through measles surveillance from 2009 to 2015 to better understand the burden of the disease in the country. Methods A descriptive analysis was made to characterize rubella cases reported through the national measles case based surveillance system. The measles case definition was used to capture potential rubella cases. A suspected measles case was a person with generalized rash and fever with cough, or coryza or conjunctivitis. Those cases whose sera were negative for measles IgM antibodies were tested for rubella IgM antibody. A confirmed rubella case was a person who tested positive for rubella IgM. Only laboratory confirmed rubella cases were analyzed in this article. Results Between 2009 and 2015, a total of 28,284 serum/plasma samples were collected and tested for measles IgM antibody and 11,151 (39.4%) were found positive. A total of 17,066 measles IgM negative or indeterminate samples were tested for rubella virus IgM and 2615 (15.3%) were found positive during the same period. Of 2615 confirmed rubella cases, 52.2% were females. The age of confirmed cases ranged from one month to 42 years with a mean age of 7.3 years. Three-fourth of all confirmed rubella cases were aged less than 10 years. The number of laboratory confirmed rubella cases linearly increased from 83 in 2009 to 856 in 2013 but dropped to 222 and 319 in 2014 and 2015 respectively. Higher number of cases occurred in the hot dry season (January through June) and in the central and western part of Ethiopia with 127 lab-confirmed outbreaks in the study period. Conclusions Based on our analysis, rubella was found to be endemic throughout Ethiopia. Children below the age of 10 years were the most affected. The burden of rubella cases varied from year to year but had a seasonal peak in March. To better understand the magnitude of rubella prior to vaccine introduction, establishing rubella surveillance system, conducting sero-prevalence studies among child bearing age females and establishing CRS sentinel surveillance among young infants are critical

Diversity and abundance of fishes in Tekeze Reservoir, Tekeze Basin, Ethiopia

The diversity and relative abundance of fishes from Tekeze Reservoir, Ethiopia, was studied from January 2016 to December 2017. Samples were collected from six sites using gill nets with different stretched mesh sizes (0.5–5.5, 6, 8, 10, 12, and 14 cm). Fish were identified to family and species level. Ecological indices such as Shannon-Weaver diversity index, Simpson’s diversity index, Species Evenness and Richness were used to analyze the data. All environmental variables were found in the optimum condition for fish production. The fish belonged to 15 species within four families: Cyprinidae, Bagridae, Claridae and Cichlidae. Shannon-Weaver and Simpson’s diversity indices ranged from 1.447 to 1.697 and 0.7333 to 0.7925, respectively, and Equitability ranged 0.6957–0.8718. Values for fish species diversity and equitability were higher (H′ = 1.715; J′ = 0.746) during the wet and dry-cold seasons, respectively. The index of relative importance(%IRI) in the gill net landings were: Oreochromis niloticus, (35.5%), Bagrus docmak (22.6%), Labeobarbus intermedius (20.5%), Labeo niloticus (10.6%)and Labeo forskalii (10.2%). The species with low relative importance (< 1%) include Clarias gariepinus, Labeobarbus nedgia, Raiamas senegalensis, Labeobarbus crassibarbis, Heterobranchus longifilis, Garra dembeensis, Bagrus bajad, Labeobarbus bynni, Labeo cylindricus, and Labeobarbus beso. The river mouth habitats had more catch composition than the pelagic. Physico-chemical parameters played a key role in the spatial variation of the fish. The study indicated decline of the fish stock in the reservoir, therefore, management plan and strategy should be in place to maintain the fish species and increase their sustainable utilization. Keywords/phrases: Abundance, Diversity, Evenness, Seasons, Tekeze Reservoi

Spatial distribution of health-risky road traffic noise pollution in Dessie City, North East Ethiopia.

ObjectiveDessie is the trade center for northeast Ethiopia. High traffic flow plus overacting of promotion made the city noisy. There is a shortage of relevant evidence that enforces policy makers to design intervention plans. Therefore, this study aimed to explore the health-risky road traffic noise pollution in Dessie City, Ethiopia.MethodsThe study was conducted by purposive selection of the study area and sampling sites of the city from May 31, 2021 -June 6, 2021. Noise level recordings were taken by a digital Sound Meter and location data was collected by Global Positioning System. Residential, health facility, commercial, and mixed sites were identified by field observation. A total of 20 noise sampling points were included. The sampling points were selected by considering World Health Organization guideline. The measurements were taken twice a day at peak hours, between 8:00-11:00am and 4:00-7:00pm on all days of the week. The sound level meter was placed at a height of 1.5m and 2m from the curb. A total of 280 sound level records were conducted over one week.ResultsAmong twenty noise recording sites, more than 50% of them registered as excessive noisy sites for all types of site categories (health facility, residential, commercial, and mixed areas). For the seven days, average noise recordings were in the range of 66-72 dB at 83% of mixed areas; 33% of health facilities; 25% of residential areas, and 86% of commercial areas. The highest levels of noise pollution were seen at the Bus-station, Buanbuawuha Square, Tekuam, Arada, Ethio General Hospital, Ersha-seble, and Menafesha areas.ConclusionThis study shows that the average noise level measurement within a week exceeded the permissible limits set by Ethiopia and the World Health Organization. It helps for policy development and timely actions against noise pollution and as baseline information for further investigation

Genetic Diversity of Potato Cultivars for Nitrogen Use Efficiency Under Contrasting Nitrogen Regimes

Developing cultivars that use nitrogen more efficiently is a sustainable strategy for reducing nitrogen use in crop production. To assess the genetic diversity for nitrogen use efficiency (NUE) and related traits in potato, a total of 97 (88 for the Western-European market and 9 Ethiopian) cultivars were evaluated at two nitrogen levels (40 kg/ha and 120 kg/ha) for 24 quantitative traits in Debre-Tabor and Injibara (Ethiopia) in the 2013 main rainy season (June to September). Highly significant variation was found among genotypes for almost all measured traits. Plant height, NUE, tuber yield, and yield-related traits and model parameters for canopy development (maximum canopy covers area under the canopy curve) were significantly affected by N levels across locations. Dutch cultivars had more rapid initial canopy development and matured earlier than the Ethiopian cultivars at both N levels and locations. A hierarchical cluster analysis grouped the cultivars in 9 and 11 genetically distinct classes at low and high N, respectively. The genetic component accounted for a large portion of the phenotypic variation for plant height, tuber number per plant, average tuber weight, and NUE under both N regimes, as indicated by a high heritability. Strong phenotypic correlations were observed between NUE and tuber number per plant, days to maturity, tuber dry matter %, maximum canopy cover, and area under the canopy curve under both low and high N conditions. The result is indicative to set the best parental line selection criteria for crossing purpose and utilize the cultivars for further potato NUE breeding programmes

Genetic Diversity of Potato Cultivars for Nitrogen Use Efficiency Under Contrasting Nitrogen Regimes

Developing cultivars that use nitrogen more efficiently is a sustainable strategy for reducing nitrogen use in crop production. To assess the genetic diversity for nitrogen use efficiency (NUE) and related traits in potato, a total of 97 (88 for the Western-European market and 9 Ethiopian) cultivars were evaluated at two nitrogen levels (40 kg/ha and 120 kg/ha) for 24 quantitative traits in Debre-Tabor and Injibara (Ethiopia) in the 2013 main rainy season (June to September). Highly significant variation was found among genotypes for almost all measured traits. Plant height, NUE, tuber yield, and yield-related traits and model parameters for canopy development (maximum canopy covers area under the canopy curve) were significantly affected by N levels across locations. Dutch cultivars had more rapid initial canopy development and matured earlier than the Ethiopian cultivars at both N levels and locations. A hierarchical cluster analysis grouped the cultivars in 9 and 11 genetically distinct classes at low and high N, respectively. The genetic component accounted for a large portion of the phenotypic variation for plant height, tuber number per plant, average tuber weight, and NUE under both N regimes, as indicated by a high heritability. Strong phenotypic correlations were observed between NUE and tuber number per plant, days to maturity, tuber dry matter %, maximum canopy cover, and area under the canopy curve under both low and high N conditions. The result is indicative to set the best parental line selection criteria for crossing purpose and utilize the cultivars for further potato NUE breeding programmes.</p

Knowledge level and factors influencing prevention of COVID-19 pandemic among residents of Dessie and Kombolcha City administrations, North-East Ethiopia: a population-based cross-sectional study

Objective In Ethiopia, community-level knowledge about the current COVID-19 pandemic has not been well studied. This study is aimed to assess knowledge level and factors influencing the prevention of the COVID-19 pandemic among residents of Dessie and Kombolcha city administrations, Ethiopia.Design Community-based cross-sectional study.Settings Dessie and Kombolcha city administrations.Participants Participants were household heads or members (n=828, &gt;18 years) who have lived in the study area for at least 2 months preceding the survey.Methods Binary logistic regression was used for a single outcome and multiple response variables. In the multivariable regression model, a value of p&lt;0.05 and adjusted OR (AOR) with 95% CI were used to identify factors associated with knowledge level of the community. Epi Info V.7.2 and SPSS V.20 software were used for data entry and analysis, respectively.Outcome Knowledge level.Results A total of 828 participants was involved with a response rate of 98%. Women were 61.7%. Participants’ mean (±SD) age was 39 (±14) years. Of the total participants 54.11% (95% CI 50.6% to 57.6%) had inadequate knowledge about COVID-19 prevention. Significant associations were reported among women (AOR=1.41; 95% CI 1.03 to 1.92); age ≥65 years (AOR=2.72; 95% CI 1.45 to 5.11); rural residence (AOR=2.69; 95% CI 1.78 to 4.07); unable to read and write (AOR=1.60; 95% CI 1.02 to 2.51); information not heard from healthcare workers, mass media and social media (AOR=1.95; 95% CI 1.35 to 2.82), (AOR=2.5; 95% CI 1.58 to 4.19) and (AOR=2.13; 95% CI 1.33 to 3.42), respectively, with inadequate knowledge.Conclusion These findings revealed that more than 50% of participants had inadequate knowledge about COVID-19. It highlights the need for widespread awareness campaigns about COVID-19 through mass media, healthcare professionals and social media as sources of information. House-to-house awareness creation is recommended to address older adults who are more vulnerable to the pandemic