Energy Consumption per GDP in Various Regions of China and Its Mode

AbstractBased on the analysis of the changes of Energy consumption per GDP in various regions of China, we can summarize modes of Energy consumption per GDP in various regions of China. The modes are Conventional mode, Gradational mode Contemporary mode, Low-carbon mode. Then, analyse the energy consumption characteristics of each mode and give optimizational initiatives

Comparative metatranscriptomic profiling and microRNA sequencing to reveal active metabolic pathways associated with a dinoflagellate bloom.

Harmful algal blooms (HABs) have increased as a result of global climate and environmental changes, exerting increasing impacts on the aquatic ecosystem, coastal economy, and human health. Despite great research efforts, our understanding on the drivers of HABs is still limited in part because HAB species’ physiology is difficult to probe in situ. Here, we used molecular ecological analyses to characterize a dinoflagellate bloom at Xiamen Harbor, China. Prorocentrum donghaiense was identified as the culprit, which nutrient bioassays showed were not nutrient-limited. Metatranscriptome profiling revealed that P. donghaiense highly expressed genes related to N- and P-nutrient uptake, phagotrophy, energy metabolism (photosynthesis, oxidative phophorylation, and rhodopsin) and carbohydrate metabolism (glycolysis/gluconeogenesis, TCA cycle and pentose phosphate) during the bloom. Many genes in P. donghaiense were up-regulated at night, including phagotrophy and environmental communication genes, and showed active expression in mitosis. Eight microbial defense genes were up-regulated in the bloom compared with previously analyzed laboratory cultures. Furthermore, 76 P. donghaiense microRNA were identified from the bloom, and their target genes exhibited marked differences in amino acid metabolism between the bloom and cultures and the potential of up-regulated antibiotic and cell communication capabilities. These findings, consistent with and complementary to recent reports, reveal major metabolic processes in P. donghaiense potentially important for bloom formation and provide a gene repertoire for developing bloom markers in future research

Comparative metatranscriptomic profiling and microRNA sequencing to reveal active metabolic pathways associated with a dinoflagellate bloom

Abstract(#br)Harmful algal blooms (HABs) have increased as a result of global climate and environmental changes, exerting increasing impacts on the aquatic ecosystem, coastal economy, and human health. Despite great research efforts, our understanding on the drivers of HABs is still limited in part because HAB species’ physiology is difficult to probe in situ . Here, we used molecular ecological analyses to characterize a dinoflagellate bloom at Xiamen Harbor, China. Prorocentrum donghaiense was identified as the culprit, which nutrient bioassays showed were not nutrient-limited. Metatranscriptome profiling revealed that P. donghaiense highly expressed genes related to N- and P-nutrient uptake, phagotrophy, energy metabolism (photosynthesis, oxidative phophorylation, and rhodopsin) and carbohydrate metabolism (glycolysis/gluconeogenesis, TCA cycle and pentose phosphate) during the bloom. Many genes in P. donghaiense were up-regulated at night, including phagotrophy and environmental communication genes, and showed active expression in mitosis. Eight microbial defense genes were up-regulated in the bloom compared with previously analyzed laboratory cultures. Furthermore, 76 P. donghaiense microRNA were identified from the bloom, and their target genes exhibited marked differences in amino acid metabolism between the bloom and cultures and the potential of up-regulated antibiotic and cell communication capabilities. These findings, consistent with and complementary to recent reports, reveal major metabolic processes in P. donghaiense potentially important for bloom formation and provide a gene repertoire for developing bloom markers in future research

Chromatin topology reorganization and transcription repression by PML-RARα in acute promyeloid leukemia.

BACKGROUND: Acute promyeloid leukemia (APL) is characterized by the oncogenic fusion protein PML-RARα, a major etiological agent in APL. However, the molecular mechanisms underlying the role of PML-RARα in leukemogenesis remain largely unknown. RESULTS: Using an inducible system, we comprehensively analyze the 3D genome organization in myeloid cells and its reorganization after PML-RARα induction and perform additional analyses in patient-derived APL cells with native PML-RARα. We discover that PML-RARα mediates extensive chromatin interactions genome-wide. Globally, it redefines the chromatin topology of the myeloid genome toward a more condensed configuration in APL cells; locally, it intrudes RNAPII-associated interaction domains, interrupts myeloid-specific transcription factors binding at enhancers and super-enhancers, and leads to transcriptional repression of genes critical for myeloid differentiation and maturation. CONCLUSIONS: Our results not only provide novel topological insights for the roles of PML-RARα in transforming myeloid cells into leukemia cells, but further uncover a topological framework of a molecular mechanism for oncogenic fusion proteins in cancers

Digital crowdsourced intervention to promote HIV testing among MSM in China: study protocol for a cluster randomized controlled trial.

BACKGROUND: Men who have sex with men (MSM) are an important HIV key population in China. However, HIV testing rates among MSM remain suboptimal. Digital crowdsourced media interventions may be a useful tool to reach this marginalized population. We define digital crowdsourced media as using social media, mobile phone applications, Internet, or other digital approaches to disseminate messages developed from crowdsourcing contests. The proposed cluster randomized controlled trial (RCT) study aims to assess the effectiveness of a digital crowdsourced intervention to increase HIV testing uptake and decrease risky sexual behaviors among Chinese MSM. METHODS: A two-arm, cluster-randomized controlled trial will be implemented in eleven cities (ten clusters) in Shandong Province, China. Targeted study participants will be 250 MSM per arm and 50 participants per cluster. MSM who are 18 years old or above, live in the study city, have not been tested for HIV in the past 3 months, are not living with HIV or have never been tested for HIV, and are willing to provide informed consent will be enrolled. Participants will be recruited through banner advertisements on Blued, the largest gay dating app in China, and in-person at community-based organizations (CBOs). The intervention includes a series of crowdsourced intervention materials (24 images and four short videos about HIV testing and safe sexual behaviors) and HIV self-test services provided by the study team. The intervention was developed through a series of participatory crowdsourcing contests before this study. The self-test kits will be sent to the participants in the intervention group at the 2nd and 3rd follow-ups. Participants will be followed up quarterly during the 12-month period. The primary outcome will be self-reported HIV testing uptake at 12 months. Secondary outcomes will include changes in condomless sex, self-test efficacy, social network engagement, HIV testing social norms, and testing stigma. DISCUSSION: Innovative approaches to HIV testing among marginalized population are urgently needed. Through this cluster randomized controlled trial, we will evaluate the effectiveness of a digital crowdsourced intervention, improving HIV testing uptake among MSM and providing a resource in related public health fields. TRIAL REGISTRATION: ChiCTR1900024350 . Registered on 6 July 2019

Extensive Promoter-Centered Chromatin Interactions Provide a Topological Basis for Transcription Regulation

Higher-order chromosomal organization for transcription regulation is poorly understood in eukaryotes. Using genome-wide Chromatin Interaction Analysis with Paired-End-Tag sequencing (ChIAPET), we mapped long-range chromatin interactions associated with RNA polymerase II in human cells and uncovered widespread promoter-centered intragenic, extragenic, and intergenic interactions. These interactions further aggregated into higher-order clusters, wherein proximal and distal genes were engaged through promoter-promoter interactions. Most genes with promoter-promoter interactions were active and transcribed cooperatively, and some interacting promoters could influence each other implying combinatorial complexity of transcriptional controls. Comparative analyses of different cell lines showed that cell-specific chromatin interactions could provide structural frameworks for cell-specific transcription, and suggested significant enrichment of enhancer-promoter interactions for cell-specific functions. Furthermore, genetically-identified disease-associated noncoding elements were found to be spatially engaged with corresponding genes through long-range interactions. Overall, our study provides insights into transcription regulation by three-dimensional chromatin interactions for both housekeeping and cell-specific genes in human cells

Land Space Ecological Restoration Zoning in Guangdong-Hong Kong-Macao Bay Area from the Perspective of Supply and Demand of Ecosystem Services

Territorial spatial ecological restoration zoning is an important basis for implementing differentiated protection and restoration strategies and an important premise for promoting the integrated protection and restoration of mountains, rivers, forests, fields, lakes, and grasses and regional coordinated governance. In the current research on territorial spatial ecological restoration zoning, less attention is paid to human demand for ecosystem services and territorial spatial ecological restoration zoning from the perspective of the supply and demand of ecosystem services. As a result, zoning is unable to solve the contradiction between human and land relations, which has a certain impact on the implementation of subsequent restoration projects. Therefore, from the perspective of ecosystem service supply and demand, this study considers the Guangdong-Hong Kong-Macao Greater Bay Area as the research area, scientifically delimiting the territorial space ecological restoration zones of the Guangdong-Hong Kong-Macao Greater Bay Area and proposes differentiated protection and restoration strategies for each zone. The results show that the spatial distribution of the comprehensive supply and demand of ecosystem services in the Guangdong-Hong Kong-Macao Greater Bay Area has a strong spatial heterogeneity. The comprehensive supply capacity mainly presented a spatial distribution pattern of high in four weeks and low in the middle, and the comprehensive demand presented a spatial distribution pattern of high in the middle and gradually decreasing from the ring to the periphery. There are many towns/streets with a spatial mismatch of supply and demand of ecosystem services (low supply and high demand; high supply and low demand), accounting for approximately 85% of the total area. There are relatively few towns/streets with spatial matching (high supply and high demand; low supply and low demand), accounting for approximately 15% of the total area. However, in space, there is a trend that the internal low-supply and high-demand areas gradually transition to the external high-supply and low-demand areas in a semicircle. According to the matching of supply and demand of ecosystem services, the Guangdong-Hong Kong-Macao Greater Bay Area was divided into four zones, and a differentiated protection and restoration strategy was proposed for each zone. In general, the key ecological conservation areas (61%) focus on nature conservation and pay attention to the maintenance of regional ecological integrity and biodiversity; the comprehensive ecological improvement zone (13%) is mainly composed of auxiliary restoration and ecological reconstruction, focusing on strengthening the construction and restoration of the suburban ecological buffer zone; the key ecological restoration areas (24%) mainly focus on auxiliary restoration and ecological remodeling to improve the ecological resilience of the area, while ecological prevention and control areas (2%) focus on nature conservation, maintaining the current ecological situation and ecological supply capacity, and preventing ecological environment degradation

Promotors or inhibitors? Role of task type on the effect of humanoid service robots on consumers’ use intention

Despite the pervasiveness of service robots in hospitality industry, it is unclear how highly human-like service robots elicit aversive effect on consumers’ use intention in addition to discomfort and when the aversive effect can be mitigated. Three experimental studies were conducted, showing that highly human-like service robots elicit greater consumer discomfort and decrease task attraction toward robots, in turn weakening consumers’ use interaction. Moreover, this research identified that emotional-social tasks (vs. mechanical tasks) mitigated the aversive effects of highly human-like service robots on consumers’ responses. The research extends the uncanny valley and mind perception theories and offers some guidelines for employing service robots with different degree of anthropomorphism. 尽管服务机器人在酒店业中非常普遍, 但尚不清楚除了不适之外,类似人类的服务机器人在多大程度上会对消费者的使用意图产生厌恶效应, 以及厌恶效应何时可以缓解. 进行了三项实验研究, 结果表明, 高度人性化的服务机器人会引起消费者更大的不适感, 降低对机器人的任务吸引力, 进而削弱消费者的使用互动. 此外, 这项研究还发现, 情感社交任务, 与机械任务相比, 减轻了高度人性化的服务机器人对消费者反应的厌恶效应. 这项研究扩展了神秘谷和心智感知理论, 并为使用具有不同程度拟人化的服务机器人提供了一些指导