Alternative antibody for the detection of CA125 antigen: a European multicenter study for the evaluation of the analytical and clinical performance of the Access (R) OV Monitor assay on the UniCel (R) Dxl 800 Immunoassay System

Background: Cancer antigen CA125 is known as a valuable marker for the management of ovarian cancer. Methods: The analytical and clinical performance of the Access OV Monitor Immunoassay System (Beckman Coulter) was evaluated at five different European sites and compared with a reference system, defined as CA125 on the Elecsys System (Roche Diagnostics). Results: Total imprecision (%CV) of the OV Monitor ranged between 3.1% and 8.8%, and inter-laboratory reproducibility between 4.7% and 5.0%. Linearity upon dilution showed a mean recovery of 100% (SD+8.1%). Endogenous interferents had no influence on OV Monitor levels (mean recoveries: hemoglobin 107%, bilirubin 103%, triglycericles 103%). There was no high-dose hook effect up to 27,193 kU/L. Clinical performance investigated in sera from 1811 individuals showed a good correlation between the Access OV Monitor and Elecsys CA125 (R = 0.982, slope = 0.921, intercept = + 1.951). OV Monitor serum levels were low in healthy individuals (n = 267, median = 9.7 kU/L, 95th percentile = 30.8 kU/L), higher in individuals with various benign diseases (n = 549, medians = 10.9-16.4 kU/L, 95th percentiles = 44.2-355 kU/L) and even higher in individuals suffering from various cancers (n = 995, medians= 12.4-445 kU/L; 95th percentiles = 53.4-4664 kU/L). Optimal diagnostic accuracy for cancer detection against the relevant benign control group by the OV Monitor was found for ovarian cancer {[}area under the curve (AUC) 0.898]. Results for the reference CA125 assay were comparable (AUC 0.899). Conclusions: The Access OV Monitor provides very good methodological characteristics and demonstrates an excellent analytical and clinical correlation with Elecsys CA125. The best diagnostic accuracy for the OV Monitor was found in ovarian cancer. Our results also suggest a clinical value of the OV Monitor in other cancers

Filtering and scalability in the ECO distributed event model

Event-based communication is useful in many application domains, ranging from small, centralised applications to large, distributed systems. Many different event models have been developed to address the requirements of different application domains. One such model is the ECO model which was designed to support distributed virtual world applications. Like many other event models, ECO has event filtering capabilities meant to improve scalability by decreasing network traffic in a distributed implementation. Our recent work in event-based systems has included building a fully distributed version of the ECO model, including event filtering capabilities. This paper describes the results of our evaluation of filters as a means of achieving increased scalability in the ECO model. The evaluation is empirical and real data gathered from an actual event-based system is used

Mindful Eating: Trait and State Mindfulness Predict Healthier Eating Behavior

Obesity and excess weight are significant societal problems. Mindfulness may encourage healthier weight and eating habits. Across four studies, we found a positive relation between mindfulness and healthier eating. Trait mindfulness was associated with less impulsive eating, reduced calorie consumption, and healthier snack choices. In addition, we found a causal effect of mindfulness on healthier eating. An experimental manipulation of state mindfulness led participants to consume fewer calories in a spontaneous eating task. We also found preliminary evidence that mindfulness affects eating behavior by encouraging attitudinal preferences for healthier foods. Taken together, these results provide strong evidence that mindfulness encourages healthier eating, even in the absence of specific instruction in mindful eating. These results suggest that generic mindfulness-based strategies could have ancillary benefits for encouraging healthier eating behavior

Hubble Space Telescope Ultraviolet Imaging and High-Resolution Spectroscopy of Water Photodissociation Products in Comet Hyakutake (C/1996 B2)

Comet Hyakutake (C/1996 B2) provided a target of opportunity for performing a systematic study of water photodissociation products in which we obtained data from three instruments on the Hubble Space Telescope (HST). The HST Goddard High Resolution Spectrograph (GHRS) was used to measure the line profile of hydrogen Lyα (H Lyα) at six locations around the coma of the comet, ranging from the nucleus to a displacement of 100,000 km, and covering different directions compared with the comet-sun line. GHRS yielded line profiles with a spectral resolution (FWHM ~4 km s^(-1)) that was a factor of 2-3 better than any previous H Lyα or Hα ground-based measurements. The Wide Field Planetary Camera 2 (WFPC2) and the Woods filter were used to obtain H Lyα images of the inner coma. The faint object spectrograph (FOS) was used to determine the OH production rate and monitor its variation throughout the HST observing sequence. The GHRS H Lyα line profiles show the behavior of a line profile that is optically thick in the core for positions near the nucleus (<5000 km) and gradually becoming more optically thin at larger displacements and lower column abundances. A composite H Lyα image constructed from four separate WFPC2 exposures is consistent with the relative fluxes seen in GHRS observations and clearly shows the dayside enhancement of a solar illuminated optically thick coma. These data were analyzed self-consistently to test our understanding of the detailed physics and chemistry of the expanding coma and our ability to obtain accurate water production rates from remote observations of gaseous hydrogen (H) and hydroxyl (OH), the major water dissociation products. Our hybrid kinetic/hydrodynamic model of the coma combined with a spherical radiative transfer calculation is able to account for (1) the velocity distribution of H atoms, (2) the spatial distribution of the H Lyα emission in the inner coma, and (3) the absolute intensities of H and OH emissions, giving a water production rate of (2.6 ± 0.4) × 10^(29) s^(-1) on 1996 April 4

An extreme ultraviolet spectrometer experiment for the Shuttle Get Away Special Program

An extreme ultraviolet (EUV) spectrometer experiment operated successfully during the STS-7 mission in an experiment to measure the global and diurnal variation of the EUV airglow. The spectrometer is an F 3.5 Wadsworth mount with mechanical collimator, a 75 x 75 mm grating, and a bare microchannel plate detector providing a spectral resolution of 7 X FWHM. Read-out of the signal is through discrete channels or resistive anode techniques. The experiment includes a microcomputer, 20 Mbit tape recorder, and a 28V, 40 Ahr silver-zinc battery. It is the first GAS payload to use an opening door. The spectrometer's 0.1 x 4.2 deg field of view is pointed vertically out of the shuttle bay. During the STS-7 flight data were acquired continuously for a period of 5 hours and 37 minutes, providing spectra of the 570 A to 850 A wavelength region of the airglow. Five diurnal cycles of the 584 A emission of neutral helium and the 834 A emission of ionized atomic oxygen were recorded. The experiment also recorded ion events and pressure pulses associated with thruster firings. The experiment is to fly again on Mission 41-F

Silicon dendritic web material

The development of a low cost and reliable contact system for solar cells and the fabrication of several solar cell modules using ultrasonic bonding for the interconnection of cells and ethylene vinyl acetate as the potting material for module encapsulation are examined. The cells in the modules were made from dendritic web silicon. To reduce cost, the electroplated layer of silver was replaced with an electroplated layer of copper. The modules that were fabricated used the evaporated Ti, Pd, Ag and electroplated Cu (TiPdAg/Cu) system. Adherence of Ni to Si is improved if a nickel silicide can be formed by heat treatment. The effectiveness of Ni as a diffusion barrier to Cu and the ease with which nickel silicide is formed is discussed. The fabrication of three modules using dendritic web silicon and employing ultrasonic bonding for interconnecting calls and ethylene vinyl acetate as the potting material is examined