The Rhetoric of Big Data: Collecting, Interpreting, and Representing in the Age of Datafication

Rhetorical studies of science, technology, and medicine (RSTM) have provided critical understanding of how argument and argument norms within a field shape what we mean by “data.” Work has also examined how questions that shape data collection are asked, how data is interpreted, and even how data is shared. Understood as a form of argument, data reveals important insights into rhetorical situations, the motives of rhetorical actors, and the broader appeals that shape everything from the kinds of technologies built, to their inclusion in our daily lives, to the infrastructures of cities, the medical practices and policies concerning public health, etc. Big data merits continued attention from RSTM scholars as our understanding of its pervasive use and its ethos grows, but its arguments remain elusive (Salvo, 2012). To unpack the elusivity of big data, we explore one particularly illustrative case of big data and political, democratic influence: the Cambridge Analytica scandal. To understand the case, we turn to social studies of data to explore the range of ethical issues raised by big data, and to examine the rhetorical strategies that entail big data

Cysteine Metallochemistry and Metal Binding: Quantification of the Thermodynamic Foundations of Cellular Homeostasis

Metals are required for life. Many metalloproteins contain cysteine in their metal-binding site (MBS) and cysteines are unique in that they are reactive, and strongly bind certain metals, which aid in metal selectivity and specificity. Using isothermal titration calorimetry (ITC), the thermodynamic foundation for metal binding, cellular protection, and transcriptional regulation, which all utilize cysteines in their MBS, are quantified. In bacteria there are metalloprotein pathways that actively uptake mercury, which are regulated by the metalloregulatory protein MerR. MerR de-represses the transcription of these mer proteins in a metal-dependent manner. Using ITC, the thermodynamic foundation of the negative allosteric coupling that regulates MerR is quantified. Within this regulated pathway is a metallochaperone in the periplasm of the cell, MerP, which functions as a mercury buffer, protecting the cell from the deleterious effects of the metal. The thermodynamic foundation of cellular protection and the mechanism of metal binding is characterized. MerP is a part of a class of metallochaperones that all contain the same protein architecture and MBS. However, this class of metallochaperones binds a wide range of metals, including zinc, copper, and mercury. How one protein scaffold with the same MBS modulates metal binding is unknown. The fundamental thermodynamics suggest that second-sphere interactions are capable of modulating metal-binding properties of these ferredoxin-like folded proteins leading to metal selectivity and specificity. Unlike bacterial mer protein, which generally bind one metal ion, other cysteine-rich proteins bind multiple metals simultaneously. In this work, the thermodynamics and mechanism of copper and zinc binding to human metallothionein (MT), a protein with many cellular functions in the regulation of metal toxicity and neuron growth inhibition, were quantified. The binding of copper, zinc, and mercury to bacterial copper storage proteins (CSPs), which also utilize a large number of cysteine residues to bind and store copper, but unlike MT are conformationally stable. Metal binding in CSPs does not alter the global protein dynamics, thus a comparison between CSPs and MT provide valuable insight into how protein dynamics and the contribution of the protein scaffold can modulate the binding of different metals in solution

Genetic Algorithm for Coalition formation April, 2010 (Unfinished)

Abstract I have developed evolutionary algorithm to solve the optimal coalition structure problem and applied it to the problem of formation of criminal gangs. A previous metho

Characterization of Iranian grapevine cultivars using microsatellite markers

Sixty-two grapevine (Vitis spp.) accessions from Iran and the USA were characterized at 9 highly polymorphic microsatellite loci using fluorescent primers and a capillary electrophoresis fragment sizing system. The number of alleles observed per locus ranged from 4 to 16 and heterozygosity values ranged from 0.47 to 0.86. Genetic similarity was estimated for each pair of accessions as the proportion of shared alleles. A phenogram constructed from genetic dissimilarity values revealed three clusters, one each for table grapes, wine grapes and rootstocks. The phenogram also revealed three clonal sets (Askari, Bidane and Yaghoti) as well as some synonyms and homonyms among Iranian table grape cultivars.

Sub-20 nm Core-Shell-Shell Nanoparticles for Bright Upconversion and Enhanced Förster Resonant Energy Transfer.

Upconverting nanoparticles provide valuable benefits as optical probes for bioimaging and Förster resonant energy transfer (FRET) due to their high signal-to-noise ratio, photostability, and biocompatibility; yet, making nanoparticles small yields a significant decay in brightness due to increased surface quenching. Approaches to improve the brightness of UCNPs exist but often require increased nanoparticle size. Here we present a unique core-shell-shell nanoparticle architecture for small (sub-20 nm), bright upconversion with several key features: (1) maximal sensitizer concentration in the core for high near-infrared absorption, (2) efficient energy transfer between core and interior shell for strong emission, and (3) emitter localization near the nanoparticle surface for efficient FRET. This architecture consists of β-NaYbF4 (core) @NaY0.8-xErxGd0.2F4 (interior shell) @NaY0.8Gd0.2F4 (exterior shell), where sensitizer and emitter ions are partitioned into core and interior shell, respectively. Emitter concentration is varied (x = 1, 2, 5, 10, 20, 50, and 80%) to investigate influence on single particle brightness, upconversion quantum yield, decay lifetimes, and FRET coupling. We compare these seven samples with the field-standard core-shell architecture of β-NaY0.58Gd0.2Yb0.2Er0.02F4 (core) @NaY0.8Gd0.2F4 (shell), with sensitizer and emitter ions codoped in the core. At a single particle level, the core-shell-shell design was up to 2-fold brighter than the standard core-shell design. Further, by coupling a fluorescent dye to the surface of the two different architectures, we demonstrated up to 8-fold improved emission enhancement with the core-shell-shell compared to the core-shell design. We show how, given proper consideration for emitter concentration, we can design a unique nanoparticle architecture to yield comparable or improved brightness and FRET coupling within a small volume

Editorial: Recent advances in hazelnut (Corylus spp.)

The European hazelnut (Corylus avellana L.) of the genus Corylus is a major species of interest for nutritional use, and its nuts are widely used worldwide in the chocolate, confectionery, and bakery industries. Other Corylus species, including C. americana, C. heterophylla, and C. colurna, are of local interest for commercial uses and provide important genetic resources for breeding. The global production of hazelnut has increased since the beginning of the last century, especially in the last 10 years in response to the demands of the confectionery industry, which processes about 90% of the harvested nuts. Cultivation of this nut has expanded in several traditional countries as well as in new areas, including Chile, South Africa, and Australia. Therefore, this Research Topic devoted to European hazelnut and its related species has been designed with the aim of assembling an effective-knowledge platform based on recent research carried out in the Corylus spp. sector. The Research Topic brings together some of the latest research outputs in hazelnut cultivation, genetic resources, and post-harvest processing, thanks to the excellent response to the call which resulted in the publication of 25 original articles. We found the edition and selection of articles for this book very inspiring and rewarding. We also thank the editorial staff and reviewers for their efforts and help during the process. The scientific contributions are briefly discussed below, grouped into seven sections according to the main topics proposed during the Research Topic launch.info:eu-repo/semantics/publishedVersio

Effective User Experience in Online Technical Communication Courses: Employing Multiple Methods within Organizational Contexts to Assess Usability

In teaching online technical communication courses, shaping an electronic interface requires extensive consideration of the user experience, both for students and for faculty members who design and teach the courses. Technical communication faculty members should provide strong examples of effective user experiences and should be leaders in making the interfaces of online learning management systems as usable as possible. Principles of usability designed for general web sites may or may not apply to learning management systems designed for educational purposes. In order to create effective online technical communication courses, one needs to consider both usability concerns and pedagogical concerns. To assess the usability and pedagogical effectiveness of online courses, faculty members may use indirect means such as heuristic analyses. In addition, they may use direct means such as usability testing, student feedback, and analytic tools. Each approach has advantages as well as limitations. Faculty members will gain the richest information through using multiple approaches. In assessing usability and pedagogical effectiveness, faculty members also need to consider the situational constraints and resources in their unique contexts. Understanding and adapting their approaches to use resources well and to work within constraints will benefit their abilities to enhance their student users' experiences with online courses

Geographic Distribution of Incompatibility Alleles in Cultivars and Selections of European Hazelnut

The european hazelnut (Corylus avellana L.) is native 24 to most of Europe and nearby areas in Asia Minor and the Caucasus Mountains. Cross-pollination is enforced by sporophytic incompatibility under the control of a single locus with multiple alleles (haplotypes). Fluorescence microscopy is routinely used to determine if a pollination is compatible or incompatible, and use of an array of known testers allows identification of the alleles of cultivars and selections. Both alleles are expressed in the stigmas, but often only one is expressed in the pollen because of dominance. Cultivars are highly heterozygous diploids (2n = 2x = 22) and clonally propagated. Most of the world's leading cultivars were selected from local wild populations near where they are now planted on a commercial scale. Genetic improvement efforts are recent and, although tremendous genetic variability is available, such efforts have had little impact outside of Oregon (USA) and France. Studies of genetic diversity using simple sequence repeat (SSR) markers have placed most cultivars in one of the four main groups: Spanish-Italian, Central European, English or Black Sea. This study presents 17 years of data on incompatibility in hazelnut, including the discovery of six new S-alleles and determination of the dominance relationships among 105 new pairs of alleles. The total number of alleles now stands at 33. The S-alleles of 284 cultivars, 13 interspecific hybrids and 522 selections of diverse origin are presented. The S-alleles identified in hazelnut cultivars is information that should be useful to breeders in the planning of crosses, to curators of germplasm collections, and to growers and nurseries as they choose cultivars and pollinizers when designing orchards. Differences in S-allele frequency seen in the cultivars and selections are related to geographic origin. The most common alleles of cultivars in the major geographical groups are S₂ in the Spanish-Italian group, S₅ in the Central European group, S₃ in the English group and S₄ in the Black Sea group. Most selections belonged to the Black Sea group, and S₄ was by far the most common allele. Differences in allele frequency were also observed among seed lots within a country.Keywords: Pollenizer, Sporophytic, Filbert, Corylus avellana, Nut breedin

Discovery, Characterization, and Linkage Mapping of Simple Sequence Repeat Markers In Hazelnut

From the genome sequence of hazelnut (Corylus avellana), 192 new polymorphic simple sequence repeat (SSR) markers were developed, characterized, and used to investigate genetic diversity in 50 accessions. Next-generation sequencing allows inexpensive sequencing of plant genomes and transcriptomes, and efficient development of polymorphic SSR markers, also known as microsatellite markers, at low cost. A search of the genome sequence of 'Jefferson' hazelnut identified 9094 fragments with long repeat motifs of 4, 5, or 6 base pairs (bp), from which polymorphic SSR markers were developed. The repeat regions in the 'Jefferson' genome were used as references to which genomic sequence reads of seven additional cultivars were aligned in silico. Visual inspection for variation in repeat number among the aligned reads identified 246 as polymorphic, for which primer pairs were designed. Polymerase chain reaction (PCR) amplification followed by agarose gel separation indicated polymorphism at 195 loci, for which fluorescent forward primers were used to amplify the DNA of 50 hazelnut accessions. Amplicons were post-PCR multiplexed for capillary electrophoresis, and allele sizes were determined for 50 accessions. After eliminating three, 192 were confirmed as polymorphic, and 169 showed only one or two alleles in each of the 50 cultivars, as expected in a diploid. At these 169 SSRs, a total of 843 alleles were found, for an average of 4.99 and a range of 2 to 17 alleles per locus. The mean observed heterozygosity, expected heterozygosity, polymorphism information content, and the frequency of null alleles were 0.51, 0.53, 0.47, and 0.03, respectively. An additional 25 primer pairs produced more than two bands in some accessions with an average of 6.8 alleles. The UPGMA dendrogram revealed a wide genetic diversity and clustered the 50 accessions according to their geographic origin. Of the new SSRs, 132 loci were placed on the linkage map. These new markers will be useful for diversity and parentage studies, cultivar fingerprinting, marker-assisted selection, and aligning the linkage map with scaffolds of the genome sequence