A Procedure for Determining the Relative Volume of Mitochondria in Hepatic Cells

About forty years ago Cowdry\u27s monograph on mitochondria appeared evaluating the work of previous investigators, indicating synonymy and, in a sense, bringing the first exploratory phase of the study of these cellular constituents to a close (1). Reasonably specific methods for their demonstration had been devised, and it was possible to have some confidence in the conclusions that the so-called mitochondria of different kinds of cells, despite dissimilarities in size or shape, were comparable cellular parts

Some Aspects of Quantitative Histology of the Liver of A-Jax Mice Before and During Tumor Induction with Carbon Tetrachloride

The proportion of the cell populations composed of parenchymal, littoral, and other types of cells is reported for control mice, mice repeatedly anesthetized, mice fed with olive oil repeatedly, and mice fed with carbon tetrachloride and olive oil, showing that the cellular components undergo a marked change when carbon tetrachloride is administered. The proportion of the liver volume occupied by parenchymal cells is found to be relatively stable throughout the period of tumor induction. The nuclear-cytoplasmic ratio of parenchymal cells is found to fall with the aging of control mice and those fed olive oil or anesthetized during the period of the experiment. The nuclear-cytoplasmic ratio is found to fall to a minimal point during the first two weeks of carbon tetrachloride feeding, thereafter gradually rising to normal values for mice of the same age, and eventually rising, after 24 feedings, to a value exceeding that of normal mice of the same age. Between 24 and 30 feedings the nuclear-cytoplasmic ratio undergoes a decrease. The volumes of parenchymal cell nuclei are found to vary with the position in a lobule, the maximal nuclear volumes being found in a region about 100μ to 200μ from the central vein in lobules of average size

3D Morphology, Ultrastructure and Development of Ceratomyxa puntazzi Stages: First Insights into the Mechanisms of Motility and Budding in the Myxozoa

Free, amoeboid movement of organisms within media as well as substrate-dependent cellular crawling processes of cells and organisms require an actin cytoskeleton. This system is also involved in the cytokinetic processes of all eukaryotic cells. Myxozoan parasites are known for the disease they cause in economical important fishes. Usually, their pathology is related to rapid proliferation in the host. However, the sequences of their development are still poorly understood, especially with regard to pre-sporogonic proliferation mechanisms. The present work employs light microscopy (LM), electron microscopy (SEM, TEM) and confocal laser scanning microscopy (CLSM) in combination with specific stains (Nile Red, DAPI, Phalloidin), to study the three-dimensional morphology, motility, ultrastructure and cellular composition of Ceratomyxa puntazzi, a myxozoan inhabiting the bile of the sharpsnout seabream