The Performance of Mangrove (Rhizophora apiculata) Leaf Extract to Treat Vibriosis (Vibrio harveyi) in Mud Crab (Scylla serrata)

Mud crabs (Scylla serrata) is one of the most economic crabs in Central Java. However, mortality was reached 80% during fattening due to bacterial infection. Meantime, mangrove leaf contain bacteriostatic and bactericidal such as saponin, flavonoid, tanin, alkaloid, steroid, terpenoid and phenol that might effective to control bacterial infection. This study was carried out to observe the performance of mangrove leaf extract to treat vibriosis in mud crabs at various concentration in vitro and in vivo. In vitro study demonstrated that mangrove leaf extract at concentration of 300 mg/l to 900 mg/l were able to prevent Vibrio harveyi growth and formed clear ring between 11.37–13.67 mm. Forty eight mud crabs at body weight of 4011±253 g were injected with a bacterial V. harveyi at density of 106 CFU/ml in between the leg join. Then immersed into mangrove leaf extract at concentration of 0 mg/l, 300 mg/l, 600 mg/l, 900 mg/l for 30 minutes. Post injection of bacterial Vibrio crabs showed clinical signs such as wide apart of swimming and walking legs, blackened carapace, white spots, and red spots. Immersion of mangrove leaf extract post injection could improved survival rate, healing and histopathological features of experimental crabs. Therefore, it can be concluded that the use of mangrove leaf extract at 900 mg/l demonstrated best survival rate, histopathological feature and recovery after 14 days post infection

Pengaruh Kepemimpinan Transformasional pada Sinisisme terhadap Perubahan Organisasional dengan Keadilan Distributif sebagai Pemoderasi

This research used transformational leadership as independent variables which had negative influence on cynicism about organizational change. While distributive justice used as moderated variable that expected to affect the relationship of transformational leadership on cynicism about organizational change. The survey was conducted to 250 employees of PT PLN branch Padang (Rayon Kuranji, Rayon Tabing, Rayon Belanti and Rayon Indarung), PT Telkom area Padang, PT MNC Sky Vision (KPP KPP Padang and Bukittinggi). At least, 202 Questionnaires were collected, but only 187 questionnaires that can be processed

Pengaruh Perceived Service Quality Terhadap Unibrand Performance Melalui Satisfaction Pada Universitas Swasta

This study refers to an earlier study that has been done by Sultan & Wong (2014). The purpose of this study was to determine the influence of Perceived Service Quality on the UniBrand Performance through Satisfaction in Private Universities. The design of this research is hypothesis testing using primary data obtained by distributing questionnaires directly to the 200 respondents who were active students of Private Universities in Jakarta. The analytical method used is Structure Equation Model (SEM). The result of this research conclude that there is a significant and positive relation between Perceived Service Quality and Satisfaction. There is a significant and positive relation between Satisfaction and UniBrand Performance. There is a significant and positive relation between Perceived Service Quality and UniBrand Performance

Pola Pengasuhan Anak pada Keluarga Tkw di Kecamatan Srengat Kabupaten Blitar

Pola asuh merupakan USAha orangtua dalam membina anak dan membimbing anak baik jiwa maupun raganya sejak lahir sampai dewasa. Ketika ibu bekerja sebagai seorang TKW diluar negeri maka seorang anak membutuhkan peran atau figur orang lain atau anggota lain sebagai pengganti ibu dalam proses sosialisasi dan internalisasi nilai. Sejauh ini belum terlalu banyak fakta yang diungkap tentang pola asuh dan proses pendidikan anak TKW yang ditinggal oleh ibu kandungnya. Sehingga perlu adanya pembahasan yang menggambarkan tentang proses pola asuh anak TKW secara mendalam. Penelitian ini dilaksanakan dengan tujuan untuk mendeskripsikan pola asuh dan proses internalisasi nilai dan norma pada anak keluarga TKW di daerah Kecamatan Srengat Kabupaten Blitar. Jenis penelitian yang digunakan adalah kualitatif deskriptif. Data diperoleh melalui wawancara, observasi, serta dokumentasi.Instrumen yang digunakan berupa pedoman wawancara maupun peneliti sendiri. Keabsahan dilakukan dengan triangulasi data. Analisis data dimulai dari reduksi data, kategorisasi, penyajian data (display), kemudian diakhiri dengan penarikan kesimpulan. Berdasarkan temuan data di lapangan diperoleh hasil bahwa pola asuh yang diterapkan orangtua wali anak TKW tidak bersifat mutlak menggunakan pola asuh otoriter, demokratis, maupun permisif. Pada dasarnya pola asuh tersebut lebih bersifat campuran. Hal ini ditinjau dari perlakuan wali terhadap anak meliputi pengelolaan keuangan, pemenuhan pendidikan, dan pemenuhan hak anak dalam berkomunikasi dengan orangtua. Kata Kunci: Pola asuh, anak,keluarga &nbsp

Meningkatkan Kemampuan Berpikir Kritis Matematis Siswa melalui Pendekatan Metacognitive Instruction

The general objective of this research is to improve critical thingking mathematically junior high school students through metacognitive Instruction approach. This study is an experimental study in SMPN 2 Tarogong Kidul with research design Non-equivalent control group pretest-postes. The results showed that the ability of mathematical critical thingking of students who received metacognitive Instruction approach to learning with better on critical thingking of students who have mathematical with conventional learning. Students' attitudes toward learning with Metacognitive Instruction approach positive

Pola Protein Sekret Kelenjar Parotoid Tiga Spesies Kodok dan Sekret Kelenjar Kulit Katak Kongkang Racun (Odorrana Hosii Boulenger,1891) melalui SDS-PAGE

Toads (Phrynoidis aspera, Ingerophrynus biporcatus, Duttaphrynus melanostictus) and poisonous rock-frog (Odorrana hosii) are members of the class Amphibian from different Families. Previously, those three species toads were in the same genus called Bufo, but now they are clustered in a different genus based on the molecular systematic. Proteins secrete from the parotoid and skin glands are one of self-defense mechanisms against to predators. The aims of this study were to determine and compare the protein patterns in secretes from toad parotoid gland and skin gland of poisonous rock-frog. The specimens were obtained from sampling at several different locations according to their habitats. Secrete collections were done by stressing both physically and chemically. Secrete was extracted by the addition of acid and sonication. The secrete protein concentration was measured by the Bradford test, whereas protein patterns were analyzed by SDS-PAGE. The protein concentrations of secrete from toad's parotoid gland are higher (Phrynoidis aspera 3.50 µg/ml; Duttaphrynus melanostictus 6.7 µg/ml Ingerophrynus biporcatus 1.32 µg/ml) than in skin gland secretion of poisonous rock- frog (1.07 µg/ml). There are 6 protein bands in parotoid gland secretion of Phrynoidis aspera, 5 protein bands in parotoid gland secretion Ingerophrynus biporcatus and 7 protein bands in secrete of Duttaphrynus melanostictus parotoid glands with molecular weight ranges 12.09-172.51 kDa. However, those proteins are different size of molecular weights for each species. Furthermore, two protein bands (with different densities) are founded in skin gland secretion of poisonous rock-frog with molecular weight of 66.17 kDa and 78.73 kDa. The differences of genes, habitats and predators may influence the differences of protein patterns in secrete from toad's paratoid gland and frog's skin glands. Those protein patterns could be useful for further studies, especially on Bufo systematic, also for exploring the potential of protein secrete as antibacterial or antifungal

Evaluation of Power Distribution based on Power Losses on Transmission Interconnection

This paper discusses the analysis of continuity of power delivery and network losses in the scenario of adding 150 kV to the Malang Raya transmission network. The discussion in this paper is based on the increasing load growth conditions in Malang Raya and the condition of the Malang 150 kV main system which is centralized in the Kebonagung Substation so that a scenario of adding 150 kV transmission network interconnection is needed to increase the capacity, reliability, and improvement of the Malang Raya system. Based on the simulation results before the scenario of adding 150 kV transmission network losses in the poor 150 kV main system by 0.02 MW, whereas after the scenario of adding 150 kV transmission network the overall losses in the 150 kV main unfortunate main system were 0.009 MW