Efeitos de extratos de Achyrocline satureioides (LAM) dc -(marcela)- sobre a reprodução de ratas

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Efeitos de extratos de Achyrocline satureioides (LAM) dc -(marcela)- sobre a reprodução de ratas

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The expression of glycosaminoglycans and proteoglycans in the uterine cervix of albino rats after local hyaluronidase infusion

Objective: To assess the local effect of hyaluronidase injection on the expression of glycosaminoglycans (GAGs) and proteoglycans (PGs) in the extracellular matrix of the uterine cervix from pregnant albino rats.Methods: Ten pregnant rats were divided into two groups on day 18 of pregnancy. the experimental group (Gexp) of rats received an intracervical infusion of 0.02 mL of hyaluronidase diluted to 1mL with distilled water, whereas the control group (Gc) received 1mL of distilled water. On day 20 of pregnancy, the pregnant rats were sacrificed and the uterine cervixes from all rats were then dissected. the qualitative expression of hyaluronic acid (HA) was assessed by immunohistochemistry and quantified by sandwich ELISA. To compare the quantitative GAG values between groups, a Student's t-test for independent samples was performed. PGs were also assessed by immunohistochemical analysis.Results: the electrophoretic profile of newly synthesized radioactively labeled GAGs degraded by specific enzymes showed that there were two predominant GAGs in both Gc and Gexp, i.e. heparan sulfate (HS) and a mixture of hondroitin sulfate (CS) and dermatan sulfate (DS). the concentrations of GAGs showed a significant reduction of CS/DS (p<0.004) and HS (p<0.005) relative to Gc. HA staining was less intense in the lamina propria and area surrounding the blood vessels in Gexp compared to Gc. the HA contents were also significantly reduced (p<0.012).Conclusions: Intracervical hyaluronidase infusion promoted a significant reduction in the concentration of sulfated GAGs as assessed by both qualitative (histochemical) and quantitative (fluorometric) measurements of HA.Fed Univ São Paulo UNIFESP, Dept Obstet, BR-05303000 São Paulo, BrazilFed Univ São Paulo UNIFESP, Dept Biochem, BR-05303000 São Paulo, BrazilFed Univ São Paulo UNIFESP, Dept Obstet, BR-05303000 São Paulo, BrazilFed Univ São Paulo UNIFESP, Dept Biochem, BR-05303000 São Paulo, BrazilWeb of Scienc

Índice de retorno ao reteste em um programa de triagem auditiva neonatal

OBJETIVO: investigar o nível de consciência dos pais em relação à importância da triagem auditiva neonatal, bem como verificar os motivos do não comparecimento ao retorno agendado após a primeira avaliação. MÉTODO: a casuística foi constituída por 31 mães e/ou responsáveis dos recém-nascidos da unidade neonatal do Hospital Universitário de Maringá, os quais não compareceram ao reteste agendado após a alta hospitalar. RESULTADOS: os motivos apresentados pelas mães e/ou responsáveis para o não comparecimento ao retorno agendado envolveram atitudes que puderam sinalizar pouca importância atribuída às questões relacionadas à audição e a Triagem Auditiva Neonatal, pois sugiram motivos irrelevantes como o esquecimento do retorno, mãe pensou que o bebê não deixaria ser submetido ao exame e a perda de horário para nova avaliação. Tal fato, provavelmente reflete a falta de conscientização por parte das mães dos recém-nascidos quanto à importância da Triagem Auditiva Neonatal. CONCLUSÃO: existe a necessidade de aumentar a conscientização geral em relação à Triagem Auditiva Neonatal, por parte dos familiares e dos profissionais que atuam diretamente com os recém-nascidos, os quais contribuirão para a agilidade do processo diagnóstico, garantindo melhores perspectiva ao futuro de crianças portadoras de deficiência auditiva

The Low Level Laser Therapy Effect on the Remodeling of Bone Extracellular Matrix

The low level laser therapy (LLLT) has been used as an option to accelerate the regeneration of bone tissue. in this study, both femurs of male Wistar rats (30 animals) were injured with a drill and the effect of LLLT using a laser diode (100 mW at 660 nm) in the bone matrix on the left paw measured. LLLT effect on the healing bone tissue matrix was evaluated by a combination of immunohistochemical histomorphometry, confocal immunofluorescence microscopy and isolation and characterization of glycosaminoglycans. Histomorphometric analysis showed that LLLT increased bone matrix and showing more organized. Alcian Blue and PAS staining seems to suggest differential glycosaminoglycans and glycoproteins. the data showed increased expression of chondroitin sulfate and hyaluronic acid, after reduction as the LLLT and mature bone, resembling the expression of osteonectin and biglycan. the difference in expression of siblings (DMP-1, OPN and BSP) is in accordance with the repair accelerated bone formation after the application of LLLT as compared with control. the expression of osteonectin and osteocalcin supports their role in bone mineralization protein, indicating that LLLT accelerates this process. the overall data show that LLLT bone changes dynamic array, shortening the time period involved in the bone repair.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal de São Paulo, Dept Orthoped & Traumatol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biochem, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Morphol & Genet, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Orthoped & Traumatol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biochem, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Morphol & Genet, São Paulo, BrazilFAPESP: 06/50452-1Web of Scienc

Effects of Training and Overtraining on Intervertebral Disc Proteoglycans

Study Design. Animal experimental study. Objective. Evaluate the effect of physical activity and overtraining condition on glycosaminoglycan concentration on the intervertebral disc (IVD) using a rat running model. Summary of Background Data. Some guidelines recommend the implementation of a physical exercise program as treatment for low back pain however, cyclic loading impact on the health of the IVD and whether there is a dose-response relationship is still incompletely understood. Methods. Thirty-two rats ages 8 weeks were divided into four groups with eight animals each. The first 8 weeks were the adaptive phase, the overtraining phase was from the ninth to the eleventh week, which consisted of increasing the number of daily training sessions from 1 to 4 and the recovery phase was represented by the 12th and 13th weeks without training. Control group 1 (CG1) did not undergo any kind of training. Control group 2 (CG2) completed just the adaptive phase. Overtraining group 1 (OT1) completed the overtraining phase. Overtraining group 2 (OT2) completed the recovery phase. Running performance tests were used to assess the "overtraining'' status of the animals. IVD glycosaminoglycans were extracted and quantified, and identified by electrophoresis. Results. Glycosaminoglycans showed a distribution between chondroitin sulfate and dermatan sulfate. Glycosaminoglycans quantification showed decreasing concentration at the following order: OT1 > CG2 > OT2 > CG1. Increased expression of dermatan sulfate was verified at the groups submitted to any training. Conclusion. Overtraining condition, as assessed by muscle and cardiovascular endurance did not lessen glycosaminoglycan concentration in the IVD. In fact, physical exercise increased glycosaminoglycan concentration in the IVD in proportion to the training load, even at overtraining condition, returning to normal levels after the recovery phase and glycosaminoglycan production is a reversible acute positive response for mechanical stimulation of the IVD.Univ Fed Sao Paulo, Dept Orthopaed & Traumatol, Escola Paulista Med, Rua Borges Lagoa,783-5 Andar Vila Clementino, BR-04038032 Sao Paulo, SP, BrazilUniv Fed Juiz de Fora, Dept Biochem, Juiz De Fora, MG, BrazilUniv Fed Sao Paulo, Dept Biochem, Escola Paulista Med, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Orthopaed & Traumatol, Escola Paulista Med, Rua Borges Lagoa,783-5 Andar Vila Clementino, BR-04038032 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Biochem, Escola Paulista Med, Sao Paulo, SP, BrazilWeb of Scienc

Changes in human intervertebral disc biochemical composition and bony end plates between middle and old age.

OBJECTIVE:This study evaluates molecular, nutritional and biochemical alterations in human intervertebral discs between middle and old age. METHODS:Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13). Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed. Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined. RESULTS:The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size. Hyaluronic acid molecular weight was around 200 kDa in all regions and ages studied. The anterior annulus differed from the posterior annulus particularly in relation to cell density and GAG content. Additionally, there were changes in the bony endplate, with fewer openings observed in the caudal than cranial endplates of all discs in both groups. CONCLUSIONS:Results show the cranial vertebral endplate is the main vascular source for the intervertebral discs. Hylauronic acid molecular weight is the same through the intervertebral disc after age of 50 years

Fucan effect on CHO cell proliferation and migration

Fucan is a term used to denominate sulfated L-fucose rich polysaccharides. Here, a heterofucan, named fucan B, was extracted from the Spatoglossum schroederi seaweed. This 21.5 kDa galactofucan inhibited CHO-Kl proliferation and migration when fibronectin was the substrate. Fucan B derivatives revealed that such effects depend on their degree of sulfation. Fucan B did not induce cell death, but promoted G1 cell cycle arrest. Western blotting and flow cytometry analysis suggest that fucan B binds to fibronectin and activates integrin, mainly integrin alpha 5 beta 1, which induces FAK/RAS/MEK/ERK activation. FAK activation inhibits CHO-K1 migration on fibronectin and ERK blocks cell cycle progression. This study indicates that fucan B could be applied in developing new antitumor drugs. (C) 2013 Elsevier B.V. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)MCTFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPERNCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Rio Grande do Norte, Dept Bioquim, BR-59072970 Natal, RN, BrazilUniversidade Federal de São Paulo, Depto Bioquim, Disciplina Biol Mol, São Paulo, BrazilUniv Fed Parana, Dept Biol Celular, BR-80060000 Curitiba, PR, BrazilUniversidade Federal de São Paulo, Depto Bioquim, Disciplina Biol Mol, São Paulo, BrazilWeb of Scienc