the molecular motion of bovine serum albumin under physiological conditions is ion specific

Specific ion effects on the Brownian motion of BSA protein under physiological conditions give new useful insights into the electrolyte–protein interactions and the molecular mechanisms involved in the Hofmeister effect

Adsorption and release of ampicillin antibiotic from ordered mesoporous silica

In this work the adsorption and the release of ampicillin - a β-lactam penicillin-like antibiotic - from MCM-41, SBA-15, and (amino functionalized) SBA-15-NH2 ordered mesoporous silica (OMS) materials were investigated. The silica matrices differ for their pore size (SBA-15 vs. MCM-41) mainly, and also for surface charge (SBA-15 and MCM-41, vs. SBA-15-NH2). OMS samples were characterized through small-angle X-rays scattering (SAXS), transmission electron microscopy (TEM), N2 adsorption–desorption isotherms, Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and potentiometric titrations. The quantification of immobilized and released ampicillin was monitored by mean of UV–Vis spectroscopy. Experimental adsorption isotherms evidenced that ampicillin's loading is not related to the pore size (dBJH) of the adsorbent. Indeed the maximal loadings were 237 mg/g for SBA-15 (dBJH = 6.5 nm), 278 mg/g for MCM-41 (dBJH = 2.2 nm), and 333 mg/g for SBA-15-NH2 (dBJH = 5.6 nm). Loading seems, instead, to be related to the surface charge density (σ) of the sorbent surface. Indeed, at pH 7.4 ampicillin drug is negatively charged and likely prefers to interact with SBA-15-NH2 (σSBA-15-NH2 = +0.223 C m−2) rather than the slightly negatively charged silicas (σSBA-15 = −0.044 C m−2 and σMCM-41 = −0.033 C m−2). Similarly, ampicillin release is affected by interfacial interactions. Indeed, we found a burst release from pure silica samples (SBA-15 and MCM-41), whereas a sustained one from SBA-15-NH2 sample. We explain this behavior as a result of an attractive interaction between the protonated amino group of SBA-15-NH2 and the negatively charged carboxylate group of ampicillin. In summary, in order to obtain a sustained drug release, the chemical nature of the matrix's surface plays a role which is more important than its textural features. SBA-15-NH2 matrix is hence a suitable candidate for local sustained release of antibiotic drugs

Misleading rites: ritual actions and <i>mechanaí</i> in Euripides (El., IT., Hel.)

Questo lavoro esamina tre casi euripidei nei quali il riferimento ad azioni rituali specifiche (il riconoscimento di un nuovo nato, la celebrazione di un rito funerario, la purificazione di un omicida) funziona come strumento di inganno utilizzato da uno o più personaggi ai danni di un altro. Si tratta di casi in cui il rito assume una precisa funzione drammatica e permette di creare una complicità con l’uditorio, che coinvolge tanto l’autore quanto coloro che, in vari momenti dell’Elettra, dell’Ifigenia tra i Tauri e dell’Elena, si trovano sulla scena.The article deals with three euripidean passages (from Electra, Iphigenia among the Taurians and Helen) in which the enactment of ritual actions (such as the admission of a newborn child to the familial group, the celebration of a funerary rite, the purification of a murderer) works as a deceiving or misleading resource. In such circumstances rites play a major dramatic role in creating a complicity between the author, the audience and the characters acting on scene.Dossier: Ritualidad en EurípidesFacultad de Humanidades y Ciencias de la Educació

Adsorption of malachite green and alizarin red S dyes using Fe-BTC metal organic framework as adsorbent

peer-reviewedSynthetic organic dyes are widely used in various industrial sectors but are also among the most harmful water pollutants. In the last decade, significant efforts have been made to develop improved materials for the removal of dyes from water, in particular, on nanostructured adsorbent materials. Metal organic frameworks (MOFs) are an attractive class of hybrid nanostructured materials with an extremely wide range of applications including adsorption. In the present work, an iron-based Fe-BTC MOF, prepared according to a rapid, aqueous-based procedure, was used as an adsorbent for the removal of alizarin red S (ARS) and malachite green (MG) dyes from water. The synthesized material was characterized in detail, while the adsorption of the dyes was monitored by UV-Vis spectroscopy. An optimal adsorption pH of 4, likely due to the establishment of favorable interactions between dyes and Fe-BTC, was found. At this pH and at a temperature of 298 K, adsorption equilibrium was reached in less than 30 min following a pseudo-second order kinetics, with k” of 4.29 × 10−3 and 3.98 × 10−2 g·mg−1 min−1 for ARS and MG, respectively. The adsorption isotherm followed the Langmuir model with maximal adsorption capacities of 80 mg·g−1(ARS) and 177 mg·g −1 (MG), and KL of 9.30·103 L·mg−1 (ARS) and 51.56·103 L·mg−1 (MG)

Cardiac Autonomic Control and Neural Arousal as Indexes of Fatigue in Professional Bus Drivers

Background: Bus driving is a mentally demanding activity that requires prolonged attention to ensure safety. The aim of the study was to assess mental fatigue caused by driving a public bus and to find a profile of workers at higher risk. Methods: We evaluated changes of critical flicker fusion (CFF) (index of central arousal) and heart rate variability (HRV) (index of autonomic balance) in a 6-hour driving shift on a real route, in 31 professional bus drivers, and we tested the influence of personal factors such as sleep quality, BMI, and age. Paired t test was used to test differences of CFF and HRV between both initial and final phase of driving, while multiple linear regression tested the influence of personal variables on the indexes of mental fatigue. Results: Results showed that CFF significantly decreased after 6 hours of bus driving (41.91 Hz, sd 3.31 vs. 41.15 Hz, sd 3.15; p = 0.041), and heart rate significantly decreased in the final phase of driving, with respect to the initial phase (85 vs. 78 bpm, p = 0.027). Increasing age (beta =-0.729, p = 0.022), risk of obstructive sleep apnea syndrome (beta =-0.530, p = 0.04), and diurnal sleepiness (beta =-0.406, p = 0.017) showed a significant effect on influencing mental fatigue. Conclusion: Elderly drivers at higher risk of sleep disorders are more prone to mental fatigue, when exposed to driving activity. Monitoring indexes of central arousal and autonomic balance, coupled with the use of structured questionnaires can represent a useful strategy to detect profile of workers at higher risk of mental fatigue in such duty

Increased shedding of soluble fragments of P-cadherin in nipple aspirate fluids from women with breast cancer.

Breast cancer, a worldwide disease with increasing incidence, develops from ductal/lobular epithelium. Nipple aspirate fluid (NAF), secreted from the breast ducts and lobules, can be analyzed to assess breast metabolic activity. P-cadherin is frequently over-expressed in high-grade invasive breast carcinomas and has been reported to be an enhancer of migration and invasion of breast cancer cells, being correlated with tumor aggressiveness. The present study analyzed the soluble fragment of P-cadherin in milk, NAF and matched plasma samples of healthy subjects and in women with precancer conditions and breast cancer. Soluble P-cadherin was detected in all plasma and milk samples, and in about 31.3% of NAF samples. The lowest levels of soluble P-cadherin were found in plasma, with no significant difference among NoCancer, PreCancer and Cancer patients. The highest concentration of soluble P-cadherin was detected in milk collected during the first trimester of lactation, significantly with respect to all NAF samples. There were significantly higher levels of soluble P-cadherin in NAF from Cancer patients than those in women with NoCancer and PreCancer (P < 0.0001). Although no significant difference was found between in situ and invasive breast cancer, soluble P-cadherin levels were found at high concentrations in c-erbB-2-positive tumors, showing a positive correlation with disease stage grouping and tumor grade, and an inverse relationship with estrogen/progesterone receptor status. High levels of the soluble fragment of P-cadherin in Cancer NAF suggest its possible release via proteolytic processing, favoring cancer cell detachment from breast duct, and suggesting that measuring soluble P-cadherin in NAF may improve the identification of women with increased breast cancer risk

An experimental analysis on driving behaviour for professional bus drivers

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Thermal diffusivity from Fourier’s inverse problem supervised by an optimization model: Theoretical analysis and experimental validation

An original experimental device coupled with an optimization technique, for determining the thermal diffusivity () of solid materials, has been devised and experimentally validated. The inverse problem of the classical Fourier heat equation in transient condition is numerically supervised by an optimization procedure for the initial and boundary conditions from measurements. Imperfect adiabaticity on the insulated lateral surfaces is explained by modeling heat loss correction functions with additional time dependent Robin conditions. The optimization model identifies the optimal values of the heat transfer coefficients and of by minimizing the residual function between the model predictions and the experimental data. Incorporating the heat loss corrections in the solution of the heat equation significantly improves the estimation of the . Indeed, the time profile of the surface temperatures measured for a specimen of PPMA material is well reflected by the simulated curves. The estimated is in good agreement with an experimental inter-comparison of eleven laboratories equipped with Laser Flash, hot disk, and hot bridge certified devices. Our results reveal a reliable capability of the model to identify the value that explains the functional dependence underlying the experimental observations. The error lies in the range 5% or 34%, depending on whether the heat losses are accounted or not

Investigating the Role of the Human Element in Maritime Accidents using Semi-Supervised Hierarchical Methods

Navigation safety is a priority both at European and global level. Despite the important progress made over the years, sea accidents remain a major concern and much work is still needed to enhance maritime safety. Knowing the causes and precursors of past accidents is essential to identify the elements on which to intervene to improve safety and reduce the possibility of an accident to occur again. In this study, 1.079 sea accidents from the International Maritime Organization (IMO) database are analyzed using Semi-supervised Recursively Partitioned Mixture Models in an attempt to identify and categorize causal themes from accident data. Special attention is devoted to the human element, which is widely recognized as a primary or precursory cause in most accidents

Genetic and environmental factors on heart rate, mean arterial pressure and carotid intima–media thickness: A longitudinal twin study

Background: Heart rate (HR), mean arterial pressure (MAP) and carotid intima–media thickness (cIMT) are moderately heritable cardiovascular traits, but the environmental effects on the longitudinal change of their heritability have never been investigated. Methods: 368 Italian and Hungarian twins (107 monozygotic, 77 dizygotic) underwent oscillometric measurement and B-mode sonography of bilateral carotid arteries in 2009/2010 and 2014. Within- -individual/cross-study wave, cross-twin/within-study wave and cross-twin/cross-study wave correlations were estimated, and bivariate Cholesky models were fitted to decompose the total variance at each wave and covariance between study waves into additive genetic, shared and unique environmental components. Results: For each trait, a moderate longitudinal stability was observed, with within-individual/crosswave correlations of 0.42 (95% CI: 0.33–0.51) for HR, 0.34 (95% CI: 0.24–0.43) for MAP, and 0.23 (95% CI: 0.12–0.33) for cIMT. Cross-twin/cross-wave correlations in monozygotic pairs were all significant and substantially higher than the corresponding dizygotic correlations. Genetic continuity was the main source of longitudinal stability, with across-time genetic correlations of 0.52 (95% CI: 0.29–0.71) for HR, 0.56 (95% CI: 0.31–0.81) for MAP, and 0.36 (95% CI: 0.07–0.64) for cIMT. Overlapping genetic factors explained respectively 57%, 77%, and 68% of the longitudinal covariance of the HR, MAP and cIMT traits. Conclusions: Genetic factors have a substantial role in the longitudinal change of HR, MAP and cIMT; however, the influence of unique environmental factors remains relevant. Further studies should better elucidate whether epigenetic mechanisms have a role in influencing the stability of the investigated traits over time