10,950 research outputs found
Latrunculin A delays anaphase onset in fission yeast by disrupting an ase1-independent pathway controlling mitotic spindle stability
It has been proposed previously that latrunculin A, an inhibitor of actin polymerization, delays the onset of anaphase by causing spindle misorientation in fission yeast. However, we show that {Delta}mto1 cells, which are defective in nucleation of cytoplasmic microtubules, have profoundly misoriented spindles but are not delayed in the timing of sister chromatid separation, providing compelling evidence that fission yeast does not possess a spindle orientation checkpoint. Instead, we show that latrunculin A delays anaphase onset by disrupting interpolar microtubule stability. This effect is abolished in a latrunculin A-insensitive actin mutant and exacerbated in cells lacking Ase1, which cross-links antiparallel interpolar microtubules at the spindle midzone both before and after anaphase. These data indicate that both Ase1 and an intact actin cytoskeleton are required for preanaphase spindle stability. Finally, we show that loss of Ase1 activates a checkpoint that requires only the Mad3, Bub1, and Mph1, but not Mad1, Mad2, or Bub3 checkpoint proteins
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Quantifying the legacy of snowmelt timing on soil greenhouse gas emissions in a seasonally dry montane forest.
The release of water during snowmelt orchestrates a variety of important belowground biogeochemical processes in seasonally snow-covered ecosystems, including the production and consumption of greenhouse gases (GHGs) by soil microorganisms. Snowmelt timing is advancing rapidly in these ecosystems, but there is still a need to isolate the effects of earlier snowmelt on soil GHG fluxes. For an improved mechanistic understanding of the biogeochemical effects of snowmelt timing during the snow-free period, we manipulated a high-elevation forest that typically receives over two meters of snowfall but little summer precipitation to influence legacy effects of snowmelt timing. We altered snowmelt rates for two years using black sand to accelerate snowmelt and white fabric to postpone snowmelt, thus creating a two- to three-week disparity in snowmelt timing. Soil microclimate and fluxes of carbon dioxide (CO2 ), methane (CH4 ), and nitrous oxide (N2 O) were monitored weekly to monthly during the snow-free period. Microbial abundances were estimated by potential assays near the end of each snow-free period. Although earlier snowmelt caused soil drying, we found no statistically significant effects (p < 0.05) of altered snowmelt timing on fluxes of CO2 or N2 O, or soil microbial abundances. Soil CH4 fluxes, however, did respond to snowmelt timing, with 18% lower rates of CH4 uptake in the earlier snowmelt treatment, but only after a dry winter. Cumulative CO2 emission and CH4 uptake were 43% and 88% greater, respectively, after the dry winter. We conclude that soil GHG fluxes can be surprisingly resistant to hydrological changes associated with earlier snowmelt, likely because of persistent moisture and microbial activities in deeper mineral soils. As a result, a drier California in the future may cause seasonally snow-covered soils in the Sierra Nevada to emit more GHGs, not less
A Machine-Checked Formalization of the Generic Model and the Random Oracle Model
Most approaches to the formal analyses of cryptographic protocols make the perfect cryptography assumption, i.e. the hypothese that there is no way to obtain knowledge about the plaintext pertaining to a ciphertext without knowing the key. Ideally, one would prefer to rely on a weaker hypothesis on the computational cost of gaining information about the plaintext pertaining to a ciphertext without knowing the key. Such a view is permitted by the Generic Model and the Random Oracle Model which provide non-standard computational models in which one may reason about the computational cost of breaking a cryptographic scheme. Using the proof assistant Coq, we provide a machine-checked account of the Generic Model and the Random Oracle Mode
An Application of The Solo Taxonomy to Classify Strategies Used by Pre-Service Teachers to Solve “One Question Problems”
: The purpose of this article is to report on the strategies of secondary mathematics pre-service teachers (PSTs) as they solved conceptually rich problems. Using the Structure of Observed Learning Outcomes by Biggs and C (1982) (SOLO) Taxonomy, 15 PSTs’ solutions (in groups of 3 or 4) were analyzed by a panel of three mathematics educators. In addition, the authors studied questions posed by PSTs during their student teaching experiences through video analysis. Questions were then categorized using Crespo’s criteria of problem posing. Results showed a significant majority of the problems posed were procedural while PSTs own problem solutions showed a lack of conceptual understanding and depth of knowledge. The authors found the SOLO Taxonomy, together with PSTs scores on the state licensure exam and Crespo’s (2003) problem posing practices criteria to be a useful combination of tools to explore connections between PSTs’ mathematical and pedagogical content knowledge
Enceladus: Cassini observations and implications for the search for life
Aims. The recent Cassini discovery of water vapor plumes ejected from the south pole of the Saturnian satellite, Enceladus, presents a unique window of opportunity for the detection of extant life in our solar system.
Methods. With its significant geothermal energy source propelling these plumes >80 km from the surface of the moon and the ensuing large temperature gradient with the surrounding environment, it is possible to have the weathering of rocks by liquid water at the rock/liquid interface. For the cases of the putatively detected salt-water oceans beneath the ice crusts of Europa and Callisto, an isolated subsurface ocean without photosynthesis or contact with an oxidizing atmosphere will approach chemical equilibrium and annihilate any ecosystems dependent on redox gradients unless there is a substantial alternative energy source. This thermodynamic tendency imposes severe constraints on any biota that is based on chemical energy. On Enceladus, the weathering of rocks by liquid water and any concomitant radioactive emissions are possible incipient conditions for life. If there is CO, CO2 and NH3 present in the spectra obtained from the plume, then this is possible evidence that amino acids could be formed at the rock/liquid interface of Enceladus. The combination of a hydrological cycle, chemical redox gradient and geochemical cycle give favorable conditions for life.
Results. We discuss the search for signatures of these species and organics in the Cassini UVIS spectra of the plume and implications for the possible detection of life
Effects of Extreme Obliquity Variations on the Habitability of Exoplanets
We explore the impact of obliquity variations on planetary habitability in
hypothetical systems with high mutual inclination. We show that large
amplitude, high frequency obliquity oscillations on Earth-like exoplanets can
suppress the ice-albedo feedback, increasing the outer edge of the habitable
zone. We restrict our exploration to hypothetical systems consisting of a
solar-mass star, an Earth-mass planet at 1 AU, and 1 or 2 larger planets. We
verify that these systems are stable for years with N-body simulations,
and calculate the obliquity variations induced by the orbital evolution of the
Earth-mass planet and a torque from the host star. We run a simplified energy
balance model on the terrestrial planet to assess surface temperature and ice
coverage on the planet's surface, and we calculate differences in the outer
edge of the habitable zone for planets with rapid obliquity variations. For
each hypothetical system, we calculate the outer edge of habitability for two
conditions: 1) the full evolution of the planetary spin and orbit, and 2) the
eccentricity and obliquity fixed at their average values. We recover previous
results that higher values of fixed obliquity and eccentricity expand the
habitable zone, but also find that obliquity oscillations further expand
habitable orbits in all cases. Terrestrial planets near the outer edge of the
habitable zone may be more likely to support life in systems that induce rapid
obliquity oscillations as opposed to fixed-spin planets. Such planets may be
the easiest to directly characterize with space-borne telescopes.Comment: 46 pages, 12 Figures, 5 Table
In patients with a previous CVA, do antioxidants protect against subsequent stroke?
Most recent randomized controlled clinical trials have not found a benefit in antioxidants (vitamin C, vitamin E, and/or beta-carotene) for preventing cardiovascular disease, including stroke. These recent clinical studies have not confirmed earlier observational studies that suggested a benefit. No studies have assessed only stroke patients and stroke outcomes. (Grade of recommendation: A, based on randomized controlled clinical trials and a systematic review of antioxidants and cardiovascular disease.
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