3,473 research outputs found
Fluctuation and localization of acoustic waves in bubbly water
Here the fluctuation properties of acoustic localization in bubbly water is
explored. We show that the strong localization can occur in such a system for a
certain frequency range and sufficient filling fractions of air-bubbles. Two
fluctuating quantities are considered, that is, the fluctuation of transmission
and the fluctuation of the phase of acoustic wave fields. When localization
occurs, these fluctuations tend to vanish, a feature able to uniquely identify
the phenomenon of wave localization.Comment: 10 pages, 4 figure
Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi.
Gene knockout strategies, RNAi and rescue experiments are all employed to study mammalian gene function. However, the disadvantages of these approaches include: loss of function adaptation, reduced viability and gene overexpression that rarely matches endogenous levels. Here, we developed an endogenous gene knockdown/rescue strategy that combines RNAi selectivity with a highly efficient CRISPR directed recombinant Adeno-Associated Virus (rAAV) mediated gene targeting approach to introduce allele-specific mutations plus an allele-selective siRNA Sensitive (siSN) site that allows for studying gene mutations while maintaining endogenous expression and regulation of the gene of interest. CRISPR/Cas9 plus rAAV targeted gene-replacement and introduction of allele-specific RNAi sensitivity mutations in the CDK2 and CDK1 genes resulted in a >85% site-specific recombination of Neo-resistant clones versus ∼8% for rAAV alone. RNAi knockdown of wild type (WT) Cdk2 with siWT in heterozygotic knockin cells resulted in the mutant Cdk2 phenotype cell cycle arrest, whereas allele specific knockdown of mutant CDK2 with siSN resulted in a wild type phenotype. Together, these observations demonstrate the ability of CRISPR plus rAAV to efficiently recombine a genomic locus and tag it with a selective siRNA sequence that allows for allele-selective phenotypic assays of the gene of interest while it remains expressed and regulated under endogenous control mechanisms
Discovery of Highly Obscured Galaxies in the Zone of Avoidance
We report the discovery of twenty-five previously unknown galaxies in the
Zone of Avoidance. Our systematic search for extended extra-galactic sources in
the GLIMPSE and MIPSGAL mid-infrared surveys of the Galactic plane has revealed
two overdensities of these sources, located around l ~ 47 and 55 degrees and
|b| less than 1 degree in the Sagitta-Aquila region. These overdensities are
consistent with the local large-scale structure found at similar Galactic
longitude and extending from |b| ~ 4 to 40 degrees. We show that the infrared
spectral energy distribution of these sources is indeed consistent with those
of normal galaxies. Photometric estimates of their redshift indicate that the
majority of these galaxies are found in the redshift range z = 0.01 - 0.05,
with one source located at z = 0.07. Comparison with known sources in the local
Universe reveals that these galaxies are located at similar overdensities in
redshift space. These new galaxies are the first evidence of a bridge linking
the large-scale structure between both sides of the Galactic plane at very low
Galactic latitude and clearly demonstrate the feasibility of detecting galaxies
in the Zone of Avoidance using mid-to-far infrared surveys.Comment: Accepted for publication in the Astronomical Journal, 28 pages, 5
tables, 11 figure
Surface energy and stability of stress-driven discommensurate surface structures
A method is presented to obtain {\it ab initio} upper and lower bounds to
surface energies of stress-driven discommensurate surface structures, possibly
non-periodic or exhibiting very large unit cells. The instability of the
stressed, commensurate parent of the discommensurate structure sets an upper
bound to its surface energy; a lower bound is defined by the surface energy of
an ideally commensurate but laterally strained hypothetical surface system. The
surface energies of the phases of the Si(111):Ga and Ge(111):Ga systems and the
energies of the discommensurations are determined within eV.Comment: 4 pages RevTeX. 2 Figures not included. Ask for a hard copy (through
regular mail) to [email protected]
The RMS Survey: Far-Infrared Photometry of Young Massive Stars
Context: The Red MSX Source (RMS) survey is a multi-wavelength campaign of
follow-up observations of a colour-selected sample of candidate massive young
stellar objects (MYSOs) in the galactic plane. This survey is returning the
largest well-selected sample of MYSOs to date, while identifying other dust
contaminant sources with similar mid-infrared colours including a large number
of new ultra-compact (UC)HII regions. Aims:To measure the far-infrared (IR)
flux, which lies near the peak of the spectral energy distribution (SED) of
MYSOs and UCHII regions, so that, together with distance information, the
luminosity of these sources can be obtained. Methods:Less than 50% of RMS
sources are associated with IRAS point sources with detections at 60 micron and
100 micron, though the vast majority are visible in Spitzer MIPSGAL or IRAS
Galaxy Atlas (IGA) images. However, standard aperture photometry is not
appropriate for these data due to crowding of sources and strong spatially
variable far-IR background emission in the galactic plane. A new technique
using a 2-dimensional fit to the background in an annulus around each source is
therefore used to obtain far-IR photometry for young RMS sources.
Results:Far-IR fluxes are obtained for a total of 1113 RMS candidates
identified as young sources. Of these 734 have flux measurements using IGA 60
micron and 100 micron images and 724 using MIPSGAL 70 micron images, with 345
having measurements in both data sets.Comment: 10 pages, 10 figures, 2 Tables, accepted to A&A. A full version of
table 1 is available from the lead author or at the CDS upon publicatio
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Affordable Near-term Burning-plasma Experiments
Fusion energy is a potential energy source for the future with plentiful fuel supplies and is expected to have benign environmental impact. The issue with fusion energy has been the scientific feasibility, and recently the cost of this approach. The key technical milestone for fusion is the achievement of a self-sustained fusion fire, ignition, in the laboratory. Despite 40 years of research and the expenditure of almost $20B worldwide, a self-sustained fusion fire has not yet been produced in the laboratory. The fusion program needs a test bed, preferably more than one, where the dynamics of a burning plasma can be studied, optimized and understood so that the engineering requirements for an engineering test reactor can be determined. Engineering and physics concepts must be developed within the next decade that will lead to an Affordable Burning Plasma Experiment if fusion is going to be perceived as making progress toward a potential long-range energy source
Picture gallery: A structured presentation of OAO-2 photometric data supported by UBV, ANS, and TD1 observations
Stellar fluxes for 531 stars in the wavelength range lambda 5500-1330A lambda are presented in the form of graphs. The stars are divided into 52 different categories on the basis of their spectral types and objects within one category are shown together. The agreement between the various ultraviolet photometric systems for early type stars is generally better than 0.10 mag. Stars with known and/or observed variability have been grouped separately. A list of stars with observed photometric properties which are indicative of stellar or interstellar anomalies is also provided
Magma Ascent along a Major Terrane Boundary: Crustal Contamination and Magma Mixing at the Drumadoon Intrusive Complex, Isle of Arran, Scotland
The composite intrusions of Drumadoon and An Cumhann crop out on the SE coast of the Isle of Arran, Scotland and form part of the larger British and Irish Palaeogene Igneous Province, a subset of the North Atlantic Igneous Province. The intrusions (shallow-level dykes and sills) comprise a central quartz-feldspar-phyric rhyolite flanked by xenocryst-bearing basaltic andesite, with an intermediate zone of dark quartz-feldspar-phyric dacite. New geochemical data provide information on the evolution of the component magmas and their relationships with each other, as well as their interaction with the crust through which they travelled. During shallow-crustal emplacement, the end-member magmas mixed. Isotopic evidence shows that both magmas were contaminated by the crust prior to mixing; the basaltic andesite magma preserves some evidence of contamination within the lower crust, whereas the rhyolite mainly records upper-crustal contamination. The Highland Boundary Fault divides Arran into two distinct terranes, the Neoproterozoic to Early Palaeozoic Grampian Terrane to the north and the Palaeozoic Midland Valley Terrane to the south. The Drumadoon Complex lies within the Midland Valley Terrane but its isotopic signatures indicate almost exclusive involvement of Grampian Terrane crust. Therefore, although the magmas originated at depth on the northern side of the Highland Boundary Fault, they have crossed this boundary during their evolution, probably just prior to emplacemen
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