3,964 research outputs found
Washburn extraction and width of the IUE point spread function
The Washburn Extraction Routine for low dispersion IUE spectra was reviewed. The shape of the point spread function (PSF) in low dispersion spectra is sufficiently well described by a gaussian function. The PSF is in large and small aperture essentially identical and values of sigma are presented. Several advantages of the extraction routine are mentioned
The CD4+ T cell methylome contributes to a distinct CD4+ T cell transcriptional signature in Mycobacterium bovis-infected cattle
peer-reviewedWe hypothesised that epigenetic regulation of CD4+ T lymphocytes contributes to a shift toward a dysfunctional T cell phenotype which may impact on their ability to clear mycobacterial infection. Combined RNA-seq transcriptomic profiling and Reduced Representation Bisulfite Sequencing identified 193 significantly differentially expressed genes and 760 differentially methylated regions (DMRs), between CD4+ T cells from M. bovis infected and healthy cattle. 196 DMRs were located within 10 kb of annotated genes, including GATA3 and RORC, both of which encode transcription factors that promote TH2 and TH17 T helper cell subsets respectively. Gene-specific DNA methylation and gene expression levels for the TNFRSF4 and Interferon-γ genes were significantly negatively correlated suggesting a regulatory relationship. Pathway analysis of DMRs identified enrichment of genes involved in the anti-proliferative TGF-β signaling pathway and TGFB1 expression was significantly increased in peripheral blood leukocytes from TB-infected cattle. This first analysis of the bovine CD4+ T cell methylome suggests that DNA methylation directly contributes to a distinct gene expression signature in CD4+ T cells from cattle infected with M. bovis. Specific methylation changes proximal to key inflammatory gene loci may be critical to the emergence of a non-protective CD4+ T cell response during mycobacterial infection in cattle
Exploring General Gauge Mediation
We explore various aspects of General Gauge Mediation(GGM). We present a
reformulation of the correlation functions used in GGM, and further elucidate
their IR and UV properties. Additionally we clarify the issue of UV sensitivity
in the calculation of the soft masses in the MSSM, highlighting the role of the
supertrace over the messenger spectrum. Finally, we present weakly coupled
messenger models which fully cover the parameter space of GGM. These examples
demonstrate that the full parameter space of GGM is physical and realizable.
Thus it should be considered a valid basis for future phenomenological
explorations of gauge mediation.Comment: 27 pages, minor changes, typos fixed in appendix
Long-Lived Neutralino NLSPs
We investigate the collider signatures of heavy, long-lived, neutral
particles that decay to charged particles plus missing energy. Specifically, we
focus on the case of a neutralino NLSP decaying to Z and gravitino within the
context of General Gauge Mediation. We show that a combination of searches
using the inner detector and the muon spectrometer yields a wide range of
potential early LHC discoveries for NLSP lifetimes ranging from 10^(-1)-10^5
mm. We further show that events from Z(l+l-) can be used for detailed kinematic
reconstruction, leading to accurate determinations of the neutralino mass and
lifetime. In particular, we examine the prospects for detailed event study at
ATLAS using the ECAL (making use of its timing and pointing capabilities)
together with the TRT, or using the muon spectrometer alone. Finally, we also
demonstrate that there is a region in parameter space where the Tevatron could
potentially discover new physics in the delayed Z(l+l-)+MET channel. While our
discussion centers on gauge mediation, many of the results apply to any
scenario with a long-lived neutral particle decaying to charged particles.Comment: 31 pages, 12 figure
RNA-seq transcriptional profiling of peripheral blood leukocytes from cattle infected with Mycobacterium bovis
Bovine tuberculosis, caused by infection with Mycobacterium bovis, is a major endemic disease affecting cattle populations worldwide, despite the implementation of stringent surveillance and control programs in many countries. The development of high-throughput functional genomics technologies, including gene expression microarrays and RNA-sequencing (RNA-seq), has enabled detailed analysis of the host transcriptome to M. bovis infection, particularly at the macrophage and peripheral blood level. In the present study, we have analyzed the peripheral blood leukocyte (PBL) transcriptome of eight natural M. bovis-infected and eight age- and sex-matched non-infected control Holstein-Friesian animals using RNA-seq. In addition, we compared gene expression profiles generated using RNA-seq with those previously generated using the high-density Affymetrix(®) GeneChip(®) Bovine Genome Array platform from the same PBL-extracted RNA. A total of 3,250 differentially expressed (DE) annotated genes were detected in the M. bovis-infected samples relative to the controls (adjusted P-value ≤0.05), with the number of genes displaying decreased relative expression (1,671) exceeding those with increased relative expression (1,579). Ingenuity(®) Systems Pathway Analysis (IPA) of all DE genes revealed enrichment for genes with immune function. Notably, transcriptional suppression was observed among several of the top-ranking canonical pathways including Leukocyte Extravasation Signaling. Comparative platform analysis demonstrated that RNA-seq detected a larger number of annotated DE genes (3,250) relative to the microarray (1,398), of which 917 genes were common to both technologies and displayed the same direction of expression. Finally, we show that RNA-seq had an increased dynamic range compared to the microarray for estimating differential gene expression
Testing the Mechanism for the LSP Stability at the LHC
The lightest supersymmetric particle (LSP) is a natural candidate for the
cold dark matter of the universe. In this Letter we discuss how to test the
mechanism responsible for the LSP stability at the LHC. We note that if
R-parity is conserved dynamically one should expect a Higgs boson which decays
mainly into two right-handed neutrinos (a "leptonic" Higgs) or into two
sfermions. The first case could exhibit spectacular lepton number violating
signals with four secondary vertices due to the long-lived nature of
right-handed neutrinos. These signals, together with the standard channels for
the discovery of SUSY, could help to establish the underlying theory at the TeV
scale.Comment: 4 pages, 3 figures, 1 table, minor corrections, published in Physics
Letters
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Broad spectrum proteomics analysis of the inferior colliculus following acute hydrogen sulfide exposure.
Acute exposure to high concentrations of H2S causes severe brain injury and long-term neurological disorders, but the mechanisms involved are not known. To better understand the cellular and molecular mechanisms involved in acute H2S-induced neurodegeneration we used a broad-spectrum proteomic analysis approach to identify key molecules and molecular pathways involved in the pathogenesis of acute H2S-induced neurotoxicity and neurodegeneration. Mice were subjected to acute inhalation exposure of up to750 ppm of H2S. H2S induced behavioral deficits and severe lesions including hemorrhage in the inferior colliculus (IC). The IC was microdissected for proteomic analysis. Tandem mass tags (TMT) liquid chromatography mass spectrometry (LC-MS/MS)-based quantitative proteomics was applied for protein identification and quantitation. LC-MS/MS identified 598, 562, and 546 altered proteomic changes at 2 h, and on days 2 and 4 post-H2S exposure, respectively. Of these, 77 proteomic changes were statistically significant at any of the 3 time points. Mass spectrometry data were subjected to Perseus 1.5.5.3 statistical analysis, and gene ontology heat map clustering. Expressions of several key molecules were verified to confirm H2S-dependent proteomics changes. Webgestalt pathway overrepresentation enrichment analysis with Panther engine revealed H2S exposure disrupted several biological processes including metabotropic glutamate receptor group 1 and inflammation mediated by chemokine and cytokine signaling pathways among others. Further analysis showed that energy metabolism, integrity of blood-brain barrier, hypoxic, and oxidative stress signaling pathways were also implicated. Collectively, this broad-spectrum proteomics data has provided important clues to follow up in future studies to further elucidate mechanisms of H2S-induced neurotoxicity
Surface energy and stability of stress-driven discommensurate surface structures
A method is presented to obtain {\it ab initio} upper and lower bounds to
surface energies of stress-driven discommensurate surface structures, possibly
non-periodic or exhibiting very large unit cells. The instability of the
stressed, commensurate parent of the discommensurate structure sets an upper
bound to its surface energy; a lower bound is defined by the surface energy of
an ideally commensurate but laterally strained hypothetical surface system. The
surface energies of the phases of the Si(111):Ga and Ge(111):Ga systems and the
energies of the discommensurations are determined within eV.Comment: 4 pages RevTeX. 2 Figures not included. Ask for a hard copy (through
regular mail) to [email protected]
Maintenance of Fertility in the Face of Meiotic Drive
Selfish genetic elements that gain a transmission advantage through the destruction of sperm have grave implications for drive male fertility. In the X-linked meiotic drive system (SR) of a stalk-eyed fly, we found that SR males have greatly enlarged testes and maintain high fertility despite the destruction of half of their sperm, even when challenged with fertilizing large numbers of females. Conversely, we observed reduced allocation of resources to the accessory glands that probably explains the lower mating frequency of SR males. Body size and eye span were also reduced, which are likely to impair viability and precopulatory success. We discuss the potential evolutionary causes of these differences between drive and standard males
Discovery of Highly Obscured Galaxies in the Zone of Avoidance
We report the discovery of twenty-five previously unknown galaxies in the
Zone of Avoidance. Our systematic search for extended extra-galactic sources in
the GLIMPSE and MIPSGAL mid-infrared surveys of the Galactic plane has revealed
two overdensities of these sources, located around l ~ 47 and 55 degrees and
|b| less than 1 degree in the Sagitta-Aquila region. These overdensities are
consistent with the local large-scale structure found at similar Galactic
longitude and extending from |b| ~ 4 to 40 degrees. We show that the infrared
spectral energy distribution of these sources is indeed consistent with those
of normal galaxies. Photometric estimates of their redshift indicate that the
majority of these galaxies are found in the redshift range z = 0.01 - 0.05,
with one source located at z = 0.07. Comparison with known sources in the local
Universe reveals that these galaxies are located at similar overdensities in
redshift space. These new galaxies are the first evidence of a bridge linking
the large-scale structure between both sides of the Galactic plane at very low
Galactic latitude and clearly demonstrate the feasibility of detecting galaxies
in the Zone of Avoidance using mid-to-far infrared surveys.Comment: Accepted for publication in the Astronomical Journal, 28 pages, 5
tables, 11 figure
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