Report D7.1 Recommendations on Safety Initiatives

A central objective of EU Kids Online is to strengthen the evidence base for policies regarding online safety in Europe. Its findings regarding children’s online experiences from across Europe offer an unrivalled opportunity to gain greater knowledge of European children’s and parents’ experiences and practices regarding risky and safer use of the internet and online technologies, thereby informing the promotion of a safer online environment for children. This chapter draws out in summary form the main implications for policy making and highlights significant issues arising from the findings of the survey, aligning them with existing initiatives where relevant in the distinct areas of risk and safety addressed. Policy actors addressed include policy makers at the European level, the Safer Internet Programme itself; Safer Internet Centres in each of the countries; national governments who play an important role in regulatory oversight; schools as central providers of internet safety training and education; industry at both national and European level as service providers and developers of children’s online content; and finally, children, young people and their parents as not only the targets for awareness-raising but who also have active roles in promoting and supporting safer internet practices

‘Think B4 U Click’: an Educational Online Safety Resource for the Irish CSPE Curriculum

Young people in Ireland, like their counterparts across Europe, are enthusiastic social networkers. EU Kids Online found that in 2010 82% of children in Ireland, aged 13-16, had a social networking (SNS) profile (O’Neill, Grehan, & Ólafsson, 2011). Social networking gives young people extraordinary opportunities to communicate with peers, share information and explore new friendships, in the relative security of an online community created through a social networking platform. Much concern has been expressed about young people\u27s apparent lack of concern about privacy issues (boyd & Marwick, 2011) and about the dangers they may be exposed to by failing to keep their SNS profiles restricted to friends or personal acquaintances. Education has been slow to address this issue in part due to the reluctance of schools to allow social networking on school networks and as well as more traditional resistance to intervene in youth cultures outside the normal curriculum

‘Think B4 U Click’: an Educational Online Safety Resource for the Irish CSPE Curriculum

Young people in Ireland, like their counterparts across Europe, are enthusiastic social networkers. EU Kids Online found that in 2010 82% of children in Ireland, aged 13-16, had a social networking (SNS) profile (O’Neill, Grehan, & Ólafsson, 2011). Social networking gives young people extraordinary opportunities to communicate with peers, share information and explore new friendships, in the relative security of an online community created through a social networking platform. Much concern has been expressed about young people\u27s apparent lack of concern about privacy issues (boyd & Marwick, 2011) and about the dangers they may be exposed to by failing to keep their SNS profiles restricted to friends or personal acquaintances. Education has been slow to address this issue in part due to the reluctance of schools to allow social networking on school networks and as well as more traditional resistance to intervene in youth cultures outside the normal curriculum

Optimized media and workflow for the expansion of human pluripotent stem cells as aggregates in suspension

3D suspension culture enables the efficient and cost-effective scale-up of human pluripotent stem cell (hPSCs) manufacturing. However, media optimized for 2D adherent cultures can lead to low volumetric productivity and inefficient workflow. To overcome these limitations we developed mTeSRTM3D, a defined medium based on mTeSRTM1, and novel protocols for fed-batch culture of hPSC aggregates. Human embryonic stem cell (hESC) lines (H1 or H9) or human induced pluripotent stem cell (hiPSC) lines (WLS-1C or STiPS-M001) that were previously maintained in 2D mTeSRTM1 culture were seeded into multiple suspension culture vessels containing mTeSRTM3D Seed Medium plus 10 μM Y-27632 ROCK inhibitor. 3D cultures were maintained using either daily 50% mTeSRTM1 medium exchanges (control) or using a fed-batch protocol whereby the culture medium was supplemented daily with mTeSRTM3D Feed Medium. After 3 or 4 days in suspension culture, aggregates were harvested, dissociated into small clumps with Gentle Cell Dissociation Reagent (GCDR) or single cell suspensions enzymatically, and re-seeded in mTeSRTM3D Seed Medium plus 10 μM Y-27632. Passaging and feeding cycles were repeated for at least 5 passages. 3D cultures were assessed for growth, viability, hPSC marker expression, in vitro differentiation potential, and karyotype. In addition, media was analyzed for molar glucose to lactate yield to characterize metabolism. By day 4, aggregates cultured in mTeSRTM3D typically grew to a mean diameter of 350 μm, with a 5-fold increase in cell number. Using mTeSRTM3D up to 109 cells can be produced from a single plate within 2-3 weeks representing a greater than 500-fold expansion. hPSC cultures maintained in mTeSRTM3D differentiated into all 3 germ layers with high efficiency. The average volumetric productivities were 0.7, 3.1 and 6.9 (x105) viable cells / mL in 2D, daily 50% media exchange, and mTeSRTM3D cultures, respectively. Using the GCDR clump passaging protocol, mTeSRTM3D cultured hPSCs retained normal karyotypes. Culture performance was evaluated in shaker bottles, spinner flasks and bioreactors. Performance in each culture system was comparable confirming straightforward scale-up and wide applicability. Typical growth rates were on the order of 1.5-fold expansion per day. Metabolic activity as assessed by the moles lactate produced to glucose consumed was 1.7, consistent with a primarily glycolytic metabolism. Image analysis was performed to estimate aggregate size during growth. Adaptation times for cells moving from 2D to 3D aggregate culture varied with different cell lines; typically one passage in 3D was required before consistent expansion passage over passage was obtained. Additionally, protocols were developed for use on a Hamilton® robotic platform for reproducible, matrix-free, high-throughput hPSC suspension culture at a small scale. mTeSRTM3D enables efficient scale-up and scale-down of hPSC cultures with greatly simplified workflow

Converting From 3.6 and 4.5 Micron Fluxes to Stellar Mass

We use high spatial resolution maps of stellar mass and infrared flux of the Large Magellanic Cloud (LMC) to calibrate a conversion between 3.6 and 4.5 micron fluxes and stellar mass, M_* = 10^{5.65} * F_{3.6}^{2.85} * F_{4.5}^{-1.85} * (D/0.05)^2 M_solar, where fluxes are in Jy and D is the luminosity distance to the source in Mpc, and to provide an approximate empirical estimate of the fractional internal uncertainty in M_* of 0.3*sqrt{N/10^6}, where N is the number of stars in the region. We find evidence that young stars and hot dust contaminate the measurements, but attempts to remove this contamination using data that is far superior than what is generally available for unresolved galaxies resulted in marginal gains in accuracy. The scatter among mass estimates for regions in the LMC is comparable to that found by previous investigators when modeling composite populations, and so we conclude that our simple conversion is as precise as possible for the data and models currently available. Our results allow for a reasonably bottom-heavy initial mass function, such as Salpeter or heavier, and moderately disfavor lighter versions such as a diet-Salpeter or Chabrier initial mass function.Comment: 7 pages, 6 figures, to be published in the Astronomical Journa

Primary care physicians’ perspectives on computer-based health risk assessment tools for chronic diseases: a mixed methods study

Background Health risk assessment tools compute an individual’s risk of developing a disease. Routine use of such tools by primary care physicians (PCPs) is potentially useful in chronic disease prevention. We sought physicians’ awareness and perceptions of the usefulness, usability and feasibility of performing assessments with computer-based risk assessment tools in primary care settings.Methods Focus groups and usability testing with a computer-based risk assessment tool were conducted with PCPs from both university-affiliated and community-based practices. Analysis was derived from grounded theory methodology.Results PCPs (n = 30) were aware of several risk assessment tools although only select tools were used routinely. The decision to use a tool depended on how use impacted practice workflow and whether the tool had credibility. Participants felt that embedding tools in the electronic medical records (EMRs) system might allow for health information from the medical record to auto-populate into the tool. User comprehension of risk could also be improved with computer-based interfaces that present risk in different formats.Conclusions In this study, PCPs chose to use certain tools more regularly because of usability and credibility. Despite there being differences in the particular tools a clinical practice used, there was general appreciation for the usefulness of tools for different clinical situations. Participants characterised particular features of an ideal tool, feeling strongly that embedding risk assessment tools in the EMR would maximise accessibility and use of the tool for chronic disease management. However, appropriate practice workflow integration and features that facilitate patient understanding at point-of-care are also essential.

Genetic testing in ALS:A survey of current practices

Objective: To determine the degree of consensus among clinicians on the clinical use of genetic testing in amyotrophic lateral sclerosis (ALS) and the factors that determine decision-making. Methods: ALS researchers worldwide were invited to participate in a detailed online survey to determine their attitudes and practices relating to genetic testing. Results: Responses from 167 clinicians from 21 different countries were analyzed. The majority of respondents (73.3%) do not consider that there is a consensus definition of familial ALS (FALS). Fifty-seven percent consider a family history of frontotemporal dementia and 48.5% the presence of a known ALS genetic mutation as sufficient for a diagnosis of FALS. Most respondents (90.2%) offer genetic testing to patients they define as having FALS and 49.4% to patients with sporadic ALS. Four main genes (SOD1, C9orf72, TARDBP, and FUS) are commonly tested. A total of 55.2% of respondents would seek genetic testing if they had personally received a diagnosis of ALS. Forty-two percent never offer presymptomatic testing to family members of patients with FALS. Responses varied between ALS specialists and nonspecialists and based on the number of new patients seen per year. Conclusions: There is a lack of consensus among clinicians as to the definition of FALS. Substantial variation exists in attitude and practices related to genetic testing of patients and presymptomatic testing of their relatives across geographic regions and between experienced specialists in ALS and nonspecialists