Effect of Passive Integrated Transponder Tag Implantation Site on Tag Retention, Growth, and Survival of Two Sizes of Juvenile Bluegills and Yellow Perch

Passive integrated transponder (PIT) tags are commonly used to monitor growth, habitat use, activity rates, and survival of individual fish. However, for successful completion of research objectives, the tags must be retained and must not affect fish growth or survival. We compared the effects of PIT tagging location on tag retention, growth, and survival of juvenile bluegills Lepomis macrochirus and yellowperch Perca flavescens. In total, 80 bluegills and 80 yellow perch from two size-classes (75–101 and 128–162 mm total length) were randomly assigned to a control or to one of three tagging location treatments: isthmus, body cavity, or dorsal musculature. Fish received daily ad libitum rations and were monitored for survival. On days 14, 28, and 42, the fish were measured, weighed, and checked for tag retention. Use of the isthmus as a tagging location resulted in lower tag retention for both species and both size-classes relative to the body cavity and dorsal musculature locations. Tagging location had no detectable effect on growth or survival responses for either species or either size-class. Thus, PIT tags that are implanted in the dorsal musculature of large juvenile bluegills and yellow perch and in the body cavity of small juvenile bluegills and yellow perch can have high retention with minimal adverse effects

Haemoglobin mass responses and performance outcomes among high-performance swimmers following a three-week Live-High, Train-High camp at 2,320m

Greater quantification and characterisation of training load (TL) throughout Live-High, Train-High (LHTH) altitude (ALT) training is required to identify periodisation strategies that may lead to physiological and performance improvements in swimmers. Purpose: This study aimed to examine the physiological responses and performance outcomes of fourteen high-performance swimmers (FINA points: 836.0 ± 35.1) following three-weeks of LHTH at 2,320m, while characterising the training load periodisation strategy adopted during the intervention. Methods: Haemoglobin (Hb) mass was measured pre-, seven- and fourteen-days post-ALT via CO rebreathing. Performance in each athlete’s primary event at national standard meets were converted to FINA points and compared from pre-to-post ALT. TL was quantified at sea level (SL) and ALT through session rating of perceived exertion (RPE), where duration of each session was multiplied by its RPE for each athlete, with all sessions totalled to give a weekly TL. Pre-to-post ALT changes were evaluated using repeated-measures ANOVA.Results: Hb mass increased significantly from 798±182g pre-ALT, to 828±187g at seven-days post (p=0.013) and 833±205g 14-days post-ALT (p=0.026). Weekly TL increased from SL (3179±638 au) during week one (4797±1349 au, p&lt;0.001) and week two (4373±967 au, p&lt;0.001), but not week three (3511±730 au, p=0.149). No evidence of improved SL swimming performance was identified. Conclusion: A periodisation strategy characterised by a sharp spike in TL followed by a slight de-load towards the end of a LHTH intervention led to improved physiological characteristics but no change in the competitive performance of high-performance swimmers.<br/

Virtualizing the Stampede2 Supercomputer with Applications to HPC in the Cloud

Methods developed at the Texas Advanced Computing Center (TACC) are described and demonstrated for automating the construction of an elastic, virtual cluster emulating the Stampede2 high performance computing (HPC) system. The cluster can be built and/or scaled in a matter of minutes on the Jetstream self-service cloud system and shares many properties of the original Stampede2, including: i) common identity management, ii) access to the same file systems, iii) equivalent software application stack and module system, iv) similar job scheduling interface via Slurm. We measure time-to-solution for a number of common scientific applications on our virtual cluster against equivalent runs on Stampede2 and develop an application profile where performance is similar or otherwise acceptable. For such applications, the virtual cluster provides an effective form of "cloud bursting" with the potential to significantly improve overall turnaround time, particularly when Stampede2 is experiencing long queue wait times. In addition, the virtual cluster can be used for test and debug without directly impacting Stampede2. We conclude with a discussion of how science gateways can leverage the TACC Jobs API web service to incorporate this cloud bursting technique transparently to the end user.Comment: 6 pages, 0 figures, PEARC '18: Practice and Experience in Advanced Research Computing, July 22--26, 2018, Pittsburgh, PA, US

Stimulated Muscle Contractions Regulate Membrane-Bound and Soluble TLR4 to Prevent LPS-Induced Signaling and Myotube Atrophy in Skeletal Muscle Cells

Toll-like receptor 4 (TLR4) activation by lipopolysaccharides (LPS) contributes to chronic inflammation and causes upregulation of muscle atrophy signaling pathways. Exercise can suppress LPS/TLR4 axis activation by reducing the expression of TLR4 on immune cells. It is unknown how this regulation occurs, and it is not clear how exercise affects TLR4 on skeletal muscle. PURPOSE: To uncover the nature and mechanisms by which exercise affects TLR4 expression and intracellular signaling using cell culture models and human experiments. METHODS: C2C12 myotubes were subjected to electrical pulse stimulation (EPS) with and without subsequent treatment with 500 ng/mL lipopolysaccharide (LPS) along with corresponding control conditions. To investigate the effect of muscle contraction on the regulation of TLR4 in-vivo, we analyzed PBMC and serum samples from eight recreationally active men that completed 60-minutes of cycling at a moderate intensity (65% of VO2max). RESULTS: In-vitro, LPS decreased membrane-bound TLR4, increased TLR4 signaling (decreased inhibitor of κBα), and induced myotube atrophy. However, stimulated muscle contractions decreased membrane-bound TLR4, increased soluble TLR4 (sTLR4), and prevented LPS-induced signaling and myotube atrophy. In human participants, a single bout of moderate-intensity exercise decreased membrane-bound TLR4 on PBMCs and increased serum-borne sTLR4. CONCLUSION: These experiments support exercise may exert a novel anti-catabolic/ anti-inflammatory effect by increasing sTLR4 and decreasing TLR4 expressed on the muscle membrane. These results could help improve interventions for conditions associated with TLR4-mediated inflammation and muscle atrophy, such as diabetes, sarcopenia, and cancer cachexia

Seagrass mapping synthesis: a resource for coastal management in the Great Barrier Reef World Heritage Area

This project provides an up to date synthesis of the available information on seagrass in the Great Barrier Reef World Heritage Area (GBRWHA). It brings together more than 30 years of spatial information and data collection into easy to use spatial GIS layers that provide key information on species, meadow type and age and reliability of the data. The project provides: Seagrass site and meadow-specific data in Geographic Information System (GIS) layers to provide seagrass data to inform research analysis and management advice. A site layer that includes >66,000 individual survey sites with information including latitude/longitude, Natural Resource Management region, site depth, seagrass presence/absence, dominant seagrass species, presence/absence of individual species, survey date, survey method, and data custodian. A meadow layer that includes 1169 individual and/or composite seagrass meadows with information including individual meadow persistence, meadow location (intertidal/subtidal), meadow density based on mean biomass and/or mean percent cover, meadow area, dominant seagrass species, seagrass species present, range of survey dates, survey method, and data custodian. Metadata to enable interpretation of the information and to identify the original data custodians for assistance with interpretation. Outcomes: This study consolidates all available seagrass spatial data for the GBRWHA collected from 1984 to December 2014 by the TropWATER Seagrass Group and CSIRO in a GIS database. It assembles and documents the state of spatial knowledge of seagrass in the GBRWHA. The spatial data is based on methods developed by TropWATER and CSIRO for seagrass habitat surveys of subtidal meadows, and TropWATER methods for intertidal surveys. Methods include sampling by boat (free divers, underwater video camera, grabs, sled with net sampling), helicopter and walking. 447,530 hectares of seagrasses were mapped (modelled deep water seagrass areas are not included in area figures in this report) within the GBRWHA; much of which provides habitat for commercial and traditional fishery species, and an important food resource for dugong and green turtle populations. Data is included for twelve seagrass species from three families. Seagrass was present at 39% of all sites visited. The study identifies areas where much of the data available for management is more than 20 years old or where there are specific habitats unsurveyed. Large areas of central and northern Queensland require updating. Several key habitat types such as reef platform seagrass meadows are poorly represented in the data