Using ³⁶Cl exposure dating to date mass movement and assess land stability on the Nicholas Range, Tasmania

Detailed mapping of dolerite slope deposits overlying sedimentary Triassic rocks on the northern slopes of the Nicholas Range in northeastern Tasmania has revealed an extensive mass movement complex. Landforms north of the summit plateau of the Nicholas Range include the following: (1) a cliff of dolerite columns with associated scree slopes at its base; (2) a topple landscape consisting of several topples that have fallen in a north-easterly direction; (3) a ripple landscape consisting of a series of long boulder ridges aligned approximately east-west. Exposure dates were obtained for three large boulders (collapsed dolerite columns) from a ridge within the ripple landscape. The two youngest dates gave a mean age of 52.1 ± 1.9 ka using36Cl. This is the estimated age for collapse of the dated columns from the cliff face c. 750 m to the south. Boulder ages and landscape morphology indicate that the ripple landscape developed by physical and chemical degradation and concurrent northern displacement of topples over a slip plane formed at the contact between dolerite colluvium and underlying Triassic sedimentary rocks. There is no evidence of movement today, other than localised debris flows associated with knickpoints in streams, and it is deduced that movement on the slip plane occurred under a cooler climate than that prevailing today, possibly under the influence of melting of winter snow during the last glacial cycle. As there is no evidence of significant recent mass movement and forests in the area are likely to have experienced many stand-destroying forest fires in the Holocene, forest harvest is not considered to pose a risk to landscape stability

Critical Interaction of Actuator Domain Residues Arginine 174, Isoleucine 188, and Lysine 205 with Modulatory Nucleotide in Sarcoplasmic Reticulum Ca 2+ -ATPase

ATP plays dual roles in the reaction cycle of the sarcoplasmic reticulum Ca2+-ATPase by acting as the phosphorylating substrate as well as in nonphosphorylating (modulatory) modes accelerating conformational transitions of the enzyme cycle. Here we have examined the involvement of actuator domain residues Arg174, Ile188, Lys204, and Lys205 by mutagenesis. Alanine mutations to these residues had little effect on the interaction of the Ca2E1 state with nucleotide or on the HnE 2 to Ca2E1 transition of the dephosphoenzyme. The phosphoenzyme processing steps, Ca2E1P to E2P and E2P dephosphorylation, and their stimulation by MgATP/ATP were markedly affected by mutations to Arg174, Ile188, and Lys205. Replacement of Ile188 with alanine abolished nucleotide modulation of dephosphorylation but not the modulation of the Ca2E1P to E2P transition. Mutation to Arg174 interfered with nucleotide modulation of either of the phosphoenzyme processing steps, indicating a significant overlap between the modulatory nucleotide-binding sites involved. Mutation to Lys205 enhanced the rates of the phosphoenzyme processing steps in the absence of nucleotide and disrupted the nucleotide modulation of the Ca2E1P to E2P transition. Remarkably, the mutants with alterations to Lys205 showed an anomalous inhibition by ATP of the dephosphorylation, and in the alanine mutant the affinity for the inhibition by ATP was indistinguishable from that for stimulation by ATP of the wild type. Hence, the actuator domain is an important player in the function of ATP as modulator of phosphoenzyme processing, with Arg174, Ile188, and Lys205 all being critically involved, although in different ways. The data support a variable site model for the modulatory effects with the nucleotide binding somewhat differently in each of the conformational states occurring during the transport cycle

Network oscillations at the boundary of an equatorial coronal hole

We investigate intensity oscillations observed simultaneously in the quiet chromosphere and in the corona, above an enhanced network area at the boundary of an equatorial coronal hole. A Fourier analysis is applied to a sequence of images observed in the 171 A and 1600 A passbands of TRACE. Four interesting features above the magnetic network are further investigated by using a wavelet analysis. Our results reveal that, in both the 171 A and 1600 A passbands, oscillations above the magnetic network show a lack of power at high frequencies (5.0-8.3 mHz), and a significant power at low (1.3-2.0 mHz) and intermediate frequencies (2.6-4.0 mHz). The global 5-min oscillation is clearly present in the 4 analyzed features when seen in the 1600 A passband, and is also found with enhanced power in feature 1 (leg of a large coronal loop) and feature 2 (legs of a coronal bright point loop) when seen in the 171 A passband. Two features above an enhanced network element (feature 3 and feature 4) show repeated propagating behaviors with a dominant period of 10 min and 5 min, respectively. We suggest these oscillations are likely to be slow magneto-acoustic waves propagating along inclined magnetic field lines, from the lower solar atmosphere into the corona. The energy flux carried by these waves is estimated of the order of 40 erg cm\^{-2} s\^{-1} for the 171 A passband and is far lower than the energy required to heat the quiet corona. For the 1600 A passband, the energy flux is about 1.4*10^6 erg cm\^{-2} s\^{-1}, which is about one third of the required energy budget for the chromosphere.Comment: 7 pages, 8 figure

Importance of Conserved N-domain Residues Thr 441 , Glu 442 , Lys 515 , Arg 560 , and Leu 562 of Sarcoplasmic Reticulum Ca 2+ -ATPase for MgATP Binding and Subsequent Catalytic Steps: PLASTICITY OF THE NUCLEOTIDE-BINDING SITE

Nine single mutations were introduced to amino acid residues Thr441, Glu442, Lys515, Arg560, Cys561, and Leu562 located in the nucleotide-binding domain of sarcoplasmic reticulum Ca2+-ATPase, and the functional consequences were studied in a direct nucleotide binding assay, as well as by steady-state and transient kinetic measurements of the overall and partial reactions of the transport cycle. Some partial reaction steps were also examined in mutants with alterations to Phe487, Arg489, and Lys492. The results implicate all these residues, except Cys561, in high affinity nucleotide binding at the substrate site. Mutations Thr441 --> Ala, Glu442 --> Ala, and Leu562 --> Phe were more detrimental to MgATP binding than to ATP binding, thus pointing to a role for these residues in the binding of Mg2+ or to a difference between the interactions with MgATP and ATP. Subsequent catalytic steps were also selectively affected by the mutations, showing the involvement of the nucleotide-binding domain in these reactions. Mutation of Arg560 inhibited phosphoryl transfer but enhanced the E1PCa2 --> E2P conformational transition, whereas mutations Thr441 --> Ala, Glu442 --> Ala, Lys492 --> Leu, and Lys515 --> Ala inhibited the E1PCa2 --> E2P transition. Hydrolysis of the E2P phosphoenzyme intermediate was enhanced in Glu442 --> Ala, Lys492 --> Leu, Lys515 --> Ala, and Arg560 --> Glu. None of the mutations affected the low affinity activation by nucleotide of the phosphoenzyme-processing steps, indicating that modulatory nucleotide interacts differently from substrate nucleotide. Mutation Glu442 --> Ala greatly enhanced reaction of Lys515 with fluorescein isothiocyanate, indicating that the two residues form a salt link in the native protein

Evidence of Explosive Evaporation in a Microflare Observed by Hinode/EIS

We present a detailed study of explosive chromospheric evaporation during a microflare which occurred on 2007 December 7 as observed with the EUV Imaging Spectrometer (EIS) onboard Hinode. We find temperature-dependent upflows for lines formed from 1.0 to 2.5 MK and downflows for lines formed from 0.05 to 0.63 MK in the impulsive phase of the flare. Both the line intensity and the nonthermal line width appear enhanced in most of the lines and are temporally correlated with the time when significant evaporation was observed. Our results are consistent with the numerical simulations of flare models, which take into account a strong nonthermal electron beam in producing the explosive chromospheric evaporation. The explosive evaporation observed in this microflare implies that the same dynamic processes may exist in events with very different magnitudes.Comment: 14 pages, 8 figures. Accepted for publication in the Astrophysical Journa

Beyond Spheroids and Discs: Classifications of CANDELS Galaxy Structure at 1.4 < z < 2 via Principal Component Analysis

Important but rare and subtle processes driving galaxy morphology and star-formation may be missed by traditional spiral, elliptical, irregular or S\'ersic bulge/disk classifications. To overcome this limitation, we use a principal component analysis of non-parametric morphological indicators (concentration, asymmetry, Gini coefficient, $M_{20}$, multi-mode, intensity and deviation) measured at rest-frame $B$-band (corresponding to HST/WFC3 F125W at 1.4 $10^{10} M_{\odot}$) galaxy morphologies. Principal component analysis (PCA) quantifies the correlations between these morphological indicators and determines the relative importance of each. The first three principal components (PCs) capture $\sim$75 per cent of the variance inherent to our sample. We interpret the first principal component (PC) as bulge strength, the second PC as dominated by concentration and the third PC as dominated by asymmetry. Both PC1 and PC2 correlate with the visual appearance of a central bulge and predict galaxy quiescence. PC1 is a better predictor of quenching than stellar mass, as as good as other structural indicators (S\'ersic-n or compactness). We divide the PCA results into groups using an agglomerative hierarchical clustering method. Unlike S\'ersic, this classification scheme separates compact galaxies from larger, smooth proto-elliptical systems, and star-forming disk-dominated clumpy galaxies from star-forming bulge-dominated asymmetric galaxies. Distinguishing between these galaxy structural types in a quantitative manner is an important step towards understanding the connections between morphology, galaxy assembly and star-formation.Comment: 31 pages, 24 figures, accepted for publication in MNRA

A mindful approach to physician self-care

There has been an increasing awareness of the importance of physician mental health. Several South African studies show a high prevalence of burnout among doctors. Burnout is characterised by three components: exhaustion, depersonalisation, and a sense of a lack of efficacy. Burnout is a result of both external and internal pressures. While lifestyle modification is essential, mindfulness-informed programmes promote self-regulation and resilience. Mindfulness programmes comprise three components: present moment awareness, perspective-taking and wisdom, and compassion. Physician wellness begins with individuals recognising the need of self-care and giving themselves permission to prioritise this. Ongoing identification of self-care needs and acting compassionately to address these needs is essential

General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH

To date, efforts to switch the cofactor specificity of oxidoreductases from nicotinamide adenine dinucleotide phosphate (NADPH) to nicotinamide adenine dinucleotide (NADH) have been made on a case-by- case basis with varying degrees of success. Here we present a straightforward recipe for altering the cofactor specificity of a class of NADPH-dependent oxidoreductases, the ketol-acid reductoisomerases (KARIs). Combining previous results for an engineered NADH-dependent variant of Escherichia coli KARI with available KARI crystal structures and a comprehensive KARI-sequence alignment, we identified key cofactor specificity determinants and used this information to construct five KARIs with reversed cofactor preference. Additional directed evolution generated two enzymes having NADH-dependent catalytic efficiencies that are greater than the wild-type enzymes with NADPH. High-resolution structures of a wild-type/variant pair reveal the molecular basis of the cofactor switch

Persistent fluctuations in stride intervals under fractal auditory stimulation

Copyright @ 2014 Marmelat et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Stride sequences of healthy gait are characterized by persistent long-range correlations, which become anti-persistent in the presence of an isochronous metronome. The latter phenomenon is of particular interest because auditory cueing is generally considered to reduce stride variability and may hence be beneficial for stabilizing gait. Complex systems tend to match their correlation structure when synchronizing. In gait training, can one capitalize on this tendency by using a fractal metronome rather than an isochronous one? We examined whether auditory cues with fractal variations in inter-beat intervals yield similar fractal inter-stride interval variability as isochronous auditory cueing in two complementary experiments. In Experiment 1, participants walked on a treadmill while being paced by either an isochronous or a fractal metronome with different variation strengths between beats in order to test whether participants managed to synchronize with a fractal metronome and to determine the necessary amount of variability for participants to switch from anti-persistent to persistent inter-stride intervals. Participants did synchronize with the metronome despite its fractal randomness. The corresponding coefficient of variation of inter-beat intervals was fixed in Experiment 2, in which participants walked on a treadmill while being paced by non-isochronous metronomes with different scaling exponents. As expected, inter-stride intervals showed persistent correlations similar to self-paced walking only when cueing contained persistent correlations. Our results open up a new window to optimize rhythmic auditory cueing for gait stabilization by integrating fractal fluctuations in the inter-beat intervals.Commission of the European Community and the Netherlands Organisation for Scientific Research