Whaling in Iron Age to post-medieval Scotland:a zooarchaeological and biomolecular study of cetacean remains from selected sites in Caithness, the Orkney Islands and the Shetland Islands

Cetacean remains have been recovered from archaeological sites all over Europe, but are especially abundant in Scotland. These remains originate from all periods and have often been worked into artefacts or tools, including chopping blocks, plaques, combs, pegs, snecks and perforated vertebral epiphyseal discs. It still remains unclear which species were exploited and to what extent active whaling was undertaken in the region. To address these questions Zooarchaeology by Mass Spectrometry (ZooMS) was undertaken on 35 cetacean specimens from five sites in Scotland (Jarlshof, Brough of Birsay, Quoygrew, Deerness and Freswick Links), dating from the Iron Age to the post-medieval period. Furthermore, morphological analysis was performed on the material in order to optimise the ZooMS identifications. A large variety of species were identified, including high numbers of Balaenidae sp and Globicephalinae sp. Comparison with other ZooMS studies in north-western Europe revealed equally high specimen numbers for these species, but also fin whale (Balaenoptera physalus), sperm whale (Physeter macrocephalus) and humpback whale (Megaptera novaeangliae). Moreover, one grey whale (Eschrichtius robustus) was identified in the Scottish specimens, adding to an increasing number of specimens indicating that the grey whale was once abundant in European waters. Furthermore, only one specimen of the common minke whale (Balaenoptera acutorostrata) was identified, despite modern stranding data which suggests this is the most common large whale species in Scottish waters. The large variety of species identified suggests that opportunistic scavenging was likely the primary method of acquiring cetaceans, though historical and ethnographic sources suggest that two distinct forms of active whaling may have occasionally been undertaken. The high number of Globicephalinae specimens from Jarlshof raise the possibility that drive-hunting might have already been undertaken at the site during the Iron Age

Medieval Whalers in the Netherlands and Flanders:Zooarchaeological Analysis of Medieval Cetacean Remains

Medieval historical sources suggest that cetacean exploitation was, for large parts of Europe, restricted to the social elite. This appears to have also been the case for the Netherlands and Flanders. It remains unclear, however, how frequently active hunting was undertaken, and which species were targeted. Zooarchaeological cetacean remains are often recovered from Medieval (AD 400-1600) sites in the Netherlands and Flanders, however the majority of these specimens have not been identified to the species level, leaving a substantial gap in our knowledge of past cetacean exploitation. By applying ZooMS, as well as morphological and osteometric analyses, these zooarchaeological specimens were identified to the species level. This analysis revealed that the North Atlantic right whale (Eubalaena glacialis), sperm whale (Physeter macrocephalus), and grey whale (Eschrichtius robustus) were frequently exploited. Active whaling appears to have been undertaken as well, especially in Flanders and in Frisia (the northern part of the Netherlands). Zooarchaeological cetacean remains appear to be present with relative frequency at high-status sites such as castles, as well as ecclesiastical sites, confirming the historical evidence that the social elite indeed did have a taste for cetacean meat. However, cetacean products were also available outside of elite and ecclesiastical contexts

Active Whaling, Opportunistic Scavenging or Long-Distance Trading:Zooarchaeological, Palaeoproteomic, and Historical Analyses on Whale Exploitation and Bone Working in Anglo-Saxon Hamwic

THE ANGLO-SAXON SITE OF HAMWIC (modern Southampton, Hampshire, UK) has been identified as a major bone-working centre. Besides antler and terrestrial mammal bone, cetacean bone has been recovered in high quantities. These specimens primarily represent working waste. Using peptide mass fingerprinting of bone collagen (ZooMS), it was determined that the majority of these specimens derive from the currently highly endangered population of North Atlantic right whale (Eubalaena glacialis). Limited historical sources appear to suggest that whaling was undertaken by the Anglo-Saxons, or by the Normans on the other side of the English Channel prior to the eleventh century ad. Nevertheless, the primary method of acquisition for whale bone was through opportunistic scavenging and trading.</p

Prospective diary study of nonpainful and painful phantom sensations in a preselected sample of child and adolescent amputees reporting phantom limbs.

Objective: To prospectively study factors associated with the occurrence of phantom sensations and pains in a pre-selected sample of child and adolescent amputees reporting phantom limbs. Design: Prospective diary study over 1 month. Participants: Fourteen child and adolescent amputees from 10-18 years of age who were missing a limb due to trauma (n = 12) or congenital limb deficiency (n = 2), and who had previously reported having phantom sensations and pain. Main Outcome Measure: Diary used to assess the occurrence of non-painful and painful phantom sensations. Items included age, sex, location and cause of amputation, past experience with stump pain and pre-amputation pain, and intensity, quality, duration, and triggers of the sensations and pains. Results: Thirteen amputees reported having 104 incidents of non-painful phantom sensations with an average intensity of 4.17 (SD = 2.14) on a 0-10 rating scale. Fifty-three incidents of phantom pain with an average intensity of 6.43 (SD = 1.76) were recorded by 8 amputees. Both amputees with a congenital limb deficiency reported phantom phenomena. Girls reported more psychosocial triggers than did boys whereas boys were more likely than girls to report that they could not identify a trigger (P = 0.0001). Boys also reported a higher proportion of physical triggers than psychosocial triggers while there were no differences for girls (P = 0.0001). Discussion: Child and adolescent amputees experience phantom sensations and pains on a regular basis over a 1-month period. Differences in triggers of phantom phenomena between boys and girls may be due to differences in activities, awareness, attribution, and willingness to report psychosocial triggers

Cannabidiol plasma determination and pharmacokinetics conducted at beginning, middle and end of long-term supplementation of a broad-spectrum hemp oil to healthy adult dogs

IntroductionVeterinary hemp products containing cannabidiol (CBD) and negligible psychoactive (THC) have increased popularity since hemp (with &lt;0.3% THC) was removed from schedule 1 substances under the Controlled Substances Act in 2018. This was accompanied by increased CBD research, mostly on the short-term safety and efficacy for inflammatory and neurological conditions. It is imperative to understand how CBD is metabolized or accumulated in the body long-term, thus the goal of the present work was to determine monthly plasma CBD concentrations, as well as changes in pharmacokinetic (PK) parameters in chronically dosed dogs.MethodsThe study was a masked, placebo-controlled, randomized design. Six adult beagles were assigned to placebo, 5 and 10 mg/kg/day CBD treatment groups. Dogs received oral oil treatment once daily for 36 weeks. Blood was collected once every 4 weeks pre- and postprandially for CBD plasma determination (at 0 and 2 h). Pharmacokinetics were conducted at 0, 18 and 36 weeks. Pharmacokinetics and monthly CBD plasma data of dogs who received CBD were analyzed as repeated measures over time using a mixed model, with significance at α = 0.05.ResultsAverage plasma CBD at 5 and 10 mg/kg were 97.3 ng/mL and 236.8 ng/mL pre-prandial, 341 ng/mL and 1,068 ng/mL postprandial, respectively. PK parameters suggested CBD accumulation over time, with significant increases in Cmax and AUC at both the 18 and 36-week timepoints. Cmax and AUC were dose proportional. Half-life demonstrated large inter-individual variations and increased (p &lt; 0.05) at weeks 18 and 36 compared to baseline. Volume of distribution was not affected by time or treatment, while MRT increased, and clearance decreased over time (p &lt; 0.05).Conclusions and clinical importanceChronic administration of CBD to healthy adult dogs led to a dose-proportional accumulation in the body for 36 weeks, which was confirmed by an increased half-life, total exposure, mean residence time and plasma peak. Our data also suggests that CBD plasma levels may have less daily variation if administered twice daily

Stable isotope evidence for dietary diversification in the pre-Columbian Amazon

Unidad de excelencia María de Maeztu CEX2019-000940-MArchaeological research is radically transforming the view that the Amazon basin and surrounding areas witnessed limited societal development before European contact. Nevertheless, uncertainty remains on the nature of the subsistence systems and the role that aquatic resources, terrestrial mammalian game, and plants had in supporting population growth, geographic dispersal, cultural adaptations and political complexity during the later stages of the pre-Columbian era. This is exacerbated by the general paucity of archaeological human remains enabling individual dietary reconstructions. Here we use stable carbon and nitrogen isotope analysis of bone collagen to reconstruct the diets of human individuals from São Luís Island (Brazilian Amazon coast) dated between ca. 1800 and 1000 cal BP and associated with distinct ceramic traditions. We expanded our analysis to include previously published data from Maracá and Marajó Island, in the eastern Amazon. Quantitative estimates of the caloric contributions from food groups and their relative nutrients using a Bayesian Mixing Model revealed distinct subsistence strategies, consisting predominantly of plants and terrestrial mammals and variably complemented with aquatic resources. This study offers novel quantitative information on the extent distinct food categories of polyculture agroforestry systems fulfilled the caloric and protein requirements of Late Holocene pre-Columbian populations in the Amazon basin

Recent dating of extinct Atlantic gray whale fossils, (Eschrichtius robustus), Georgia Bight and Florida, western Atlantic Ocean

The Atlantic gray whale (Eschrichtius robustus) presents an interesting case study of climate related dispersal and extinction. While (limited) fossil records confirm its presence in the Atlantic up until the 18th Century, its abundance and distribution within the Eastern and Western basins are still not well understood. The discovery of presumed gray whale fossil remains from the Georgia Bight and the Atlantic coast of Florida, from the mid-1980s to late-2000s, provides a new opportunity to recover additional data regarding their chronology within the Western basin. Here, we apply accelerator mass spectrometry radiocarbon techniques to six fossil whale finds, identifying dates within marine isotope stage 3 (59–24 ka) and the late Holocene, ∼2,000 yr BP. We additionally confirm the taxonomic identification of two fossil bone samples as E. robustus using collagen peptide mass fingerprinting (ZooMS). The obtained dates, when combined with a larger corpus of previously published Atlantic gray whale fossil dates, support the hypothesis for the decline of the Atlantic gray whale in the late Pleistocene and the late Holocene. These new data augment the findings of the Eastern Atlantic Basin and better incorporate the Western Atlantic Basin into a pan-ocean understanding for the species

Barcoding the largest animals on Earth: ongoing challenges and molecular solutions in the taxonomic identification of ancient cetaceans.

Over the last few centuries, many cetacean species have witnessed dramatic global declines due to industrial overharvesting and other anthropogenic influences, and thus are key targets for conservation. Whale bones recovered from archaeological and palaeontological contexts can provide essential baseline information on the past geographical distribution and abundance of species required for developing informed conservation policies. Here we review the challenges with identifying whale bones through traditional anatomical methods, as well as the opportunities provided by new molecular analyses. Through a case study focused on the North Sea, we demonstrate how the utility of this (pre)historic data is currently limited by a lack of accurate taxonomic information for the majority of ancient cetacean remains. We then discuss current opportunities presented by molecular identification methods such as DNA barcoding and collagen peptide mass fingerprinting (zooarchaeology by mass spectrometry), and highlight the importance of molecular identifications in assessing ancient species' distributions through a case study focused on the Mediterranean. We conclude by considering high-throughput molecular approaches such as hybridization capture followed by next-generation sequencing as cost-effective approaches for enhancing the ecological informativeness of these ancient sample sets.This article is part of the themed issue 'From DNA barcodes to biomes'

Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome.

The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for food during the winter, and while stable isotope analyses have revealed dietary similarities at some sites, deciphering the details of provisioning strategies have been challenging. In this study, we apply zooarchaeology by mass spectrometry (ZooMS) and liquid chromatography tandem mass spectrometry to dog palaeofaeces to investigate protein preservation in this highly degradable material and obtain information about the diet of domestic dogs at the Nunalleq site, Alaska. We identify a suite of digestive and metabolic proteins from the host species, demonstrating the utility of this material as a novel and viable substrate for the recovery of gastrointestinal proteomes. The recovered proteins revealed that the Nunalleq dogs consumed a range of Pacific salmon species (coho, chum, chinook and sockeye) and that the consumed tissues derived from muscle and bone tissues as well as roe and guts. Overall, the study demonstrated the viability of permafrost-preserved palaeofaeces as a unique source of host and dietary proteomes.DNRF, E

Shepherding the past: High-resolution data on Neolithic Southern Iberian livestock management at Cueva de El Toro (Antequera, Málaga)

The feeding strategies of the first domesticated herds had to manage the risks arising from the novelty of livestock practices in territories often distant from the animals’ primary habitats. The Iberian Peninsula is characterised by a great diversity of environments, which undoubtedly influenced these dynamics. At the beginning of the Neolithic period these led the possibility to combine diverse livestock farming practices based on different animal feeding habits. This variability is also consistent with the rythms of adoption of domesticated animals, being later on the northern area. In order to address this issue, this work focuses on the dietary regimes of early sheep herds from southern Iberia, an area for which information is currently scarce. This study utilises high-resolution radiocarbon dating and stable isotope data on teeth to investigate sheep husbandry management strategies in Cueva de El Toro (Antequera, Málaga). The radiocarbon dates on the analysed remains evidenced they were deposited at the site over a short period, supporting the recurrent use of the cave. The sequential analysis of oxygen and carbon isotopes in tooth enamel reveals distinct livestock management strategies, reproduction patterns, feeding habits, and mobility during this short period. This variability demonstrates that livestock management practices in the western Mediterranean are more diverse than previously considered. Furthermore, these findings support the hypothesis that early Neolithic communities in the southern Iberian Peninsula were able to adopt different feeding strategies within the same herd, depending on their ecological and productive needs