3,147 research outputs found
Effect of Continuous BFR Exercise on Tissues Oxygenation Characteristics in Incomplete Spinal Cord Injured Patients
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An Overview of Infections in Cystic Fibrosis Airways and the Role of Environmental Conditions on Pseudomonas aeruginosa Biofilm Formation and Viability
In this chapter, the authors review a major complication associated with cystic fibrosis (CF), problematic bacterial infections of the lungs. Infection by organisms such as Staphylococcus aureus, Burkholderia cepacia complex, and Pseudomonas aeruginosa (a major player in CF related infections) results in complications due to increased inflammation and production of virulence factors produced by the bacteria. In addition to these more canonical organisms associated with CF infection, emergingbacterial species have been found in the CF, including anaerobes that have only within the past 5-10 years have been reported to exist in the lungs. P. aeruginosa has long been a cause of devastating infections, and is often seen as the“hallmark”organism associated with the disease. The authors describe the P. aeruginosa infection, including its conversion to a mucoid phenotype, as well as its ability to utilize the thicker airway surface layer associated with CF to grow in “mode two biofilms.” Finally, the authors discuss treatments for bacterial infections, and some of the new advances that offerhope for treatment of CF symptoms and infections by multi-drug resistant organisms. Among these new treatments is the application of acidified nitrite, a non-antibiotic treatment that has been found to be effective at killing nonmucoid and mucoid variants of P. aeruginosa
Re-entrant ferroelectricity in liquid crystals
The ferroelectric (Sm C) -- antiferroelectric (Sm C) -- reentrant
ferroelectric (re Sm C) phase temperature sequence was observed for system
with competing synclinic - anticlinic interactions. The basic properties of
this system are as follows (1) the Sm C phase is metastable in temperature
range of the Sm C stability (2) the double inversions of the helix
handedness at Sm C -- Sm C and Sm C% -- re-Sm C phase
transitions were found (3) the threshold electric field that is necessary to
induce synclinic ordering in the Sm C phase decreases near both Sm
C -- Sm C and Sm C -- re-Sm C phase boundaries, and it has
maximum in the middle of the Sm C stability region. All these properties
are properly described by simple Landau model that accounts for nearest
neighboring layer steric interactions and quadrupolar ordering only.Comment: 10 pages, 5 figures, submitted to PR
In-beam gamma-ray spectroscopy of 35Mg and 33Na
Excited states in the very neutron-rich nuclei 35Mg and 33Na were populated
in the fragmentation of a 38Si projectile beam on a Be target at 83 MeV/u beam
energy. We report on the first observation of gamma-ray transitions in 35Mg,
the odd-N neighbor of 34Mg and 36Mg, which are known to be part of the "Island
of Inversion" around N = 20. The results are discussed in the framework of
large- scale shell-model calculations. For the A = 3Z nucleus 33Na, a new
gamma-ray transition was observed that is suggested to complete the gamma-ray
cascade 7/2+ --> 5/2+ --> 3/2+ gs connecting three neutron 2p-2h intruder
states that are predicted to form a close-to-ideal K = 3/2 rotational band in
the strong-coupling limit.Comment: Accepted for publication Phys. Rev. C. March 16, 2011: Replaced
figures 3 and 5. We thank Alfredo Poves for pointing out a problem with the
two figure
Thin films of Type 1 collagen for cell by cell analysis of morphology and tenascin-C promoter activity
BACKGROUND: The use of highly reproducible and spatiallyhomogeneous thin film matrices permits automated microscopy and quantitative determination of the response of hundreds of cells in a population. Using thin films of extracellular matrix proteins, we have quantified, on a cell-by-cell basis, phenotypic parameters of cells on different extracellular matrices. We have quantitatively examined the relationship between fibroblast morphology and activation of the promoter for the extracellular matrix protein tenascin-C using a tenascin-C promoter-based GFP reporter construct. RESULTS: We find that when considering the average response from the population of cells, cell area correlates with tenascin-C promoter activity as has been previously suggested; however cell-by-cell analysis suggests that cell area and promoter activity are not tightly correlated within individual cells. CONCLUSION: This study demonstrates how quantitative cell-by-cell analysis, facilitated by the use of thin films of extracellular matrix proteins, can provide insight into the relationship between phenotypic parameters
Temperature dependence of binary and ternary recombination of H3+ ions with electron
We study binary and the recently discovered process of ternary He-assisted
recombination of H3+ ions with electrons in a low temperature afterglow plasma.
The experiments are carried out over a broad range of pressures and
temperatures of an afterglow plasma in a helium buffer gas. Binary and
He-assisted ternary recombination are observed and the corresponding
recombination rate coefficients are extracted for temperatures from 77 K to 330
K. We describe the observed ternary recombination as a two-step mechanism:
First, a rotationally-excited long-lived neutral molecule H3* is formed in
electron-H3+ collisions. Second, the H3* molecule collides with a helium atom
that leads to the formation of a very long-lived Rydberg state with high
orbital momentum. We present calculations of the lifetimes of H3* and of the
ternary recombination rate coefficients for para and ortho-H3+. The
calculations show a large difference between the ternary recombination rate
coefficients of ortho- and para-H3+ at temperatures below 300 K. The measured
binary and ternary rate coefficients are in reasonable agreement with the
calculated values.Comment: 15 page
The Anti-Sigma Factor MucA of Pseudomonas aeruginosa: Dramatic Differences of a mucA22 vs. a ΔmucA Mutant in Anaerobic Acidified Nitrite Sensitivity of Planktonic and Biofilm Bacteria in vitro and During Chronic Murine Lung Infection
Mucoid mucA22 Pseudomonas aeruginosa (PA) is an opportunistic lung pathogen of cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD) patients that is highly sensitive to acidified nitrite (A-NO2-). In this study, we first screened PA mutant strains for sensitivity or resistance to 20 mM A-NO2- under anaerobic conditions that represent the chronic stages of the aforementioned diseases. Mutants found to be sensitive to A-NO2- included PA0964 (pmpR, PQS biosynthesis), PA4455 (probable ABC transporter permease), katA (major catalase, KatA) and rhlR (quorum sensing regulator). In contrast, mutants lacking PA0450 (a putative phosphate transporter) and PA1505 (moaA2) were A-NO2- resistant. However, we were puzzled when we discovered that mucA22 mutant bacteria, a frequently isolated mucA allele in CF and to a lesser extent COPD, were more sensitive to A-NO2- than a truncated ΔmucA deletion (Δ157–194) mutant in planktonic and biofilm culture, as well as during a chronic murine lung infection. Subsequent transcriptional profiling of anaerobic, A-NO2--treated bacteria revealed restoration of near wild-type transcript levels of protective NO2- and nitric oxide (NO) reductase (nirS and norCB, respectively) in the ΔmucA mutant in contrast to extremely low levels in the A-NO2--sensitive mucA22 mutant. Proteins that were S-nitrosylated by NO derived from A-NO2- reduction in the sensitive mucA22 strain were those involved in anaerobic respiration (NirQ, NirS), pyruvate fermentation (UspK), global gene regulation (Vfr), the TCA cycle (succinate dehydrogenase, SdhB) and several double mutants were even more sensitive to A-NO2-. Bioinformatic-based data point to future studies designed to elucidate potential cellular binding partners for MucA and MucA22. Given that A-NO2- is a potentially viable treatment strategy to combat PA and other infections, this study offers novel developments as to how clinicians might better treat problematic PA infections in COPD and CF airway diseases
Inverse-kinematics one-neutron pickup with fast rare-isotope beams
New measurements and reaction model calculations are reported for single
neutron pickup reactions onto a fast \nuc{22}{Mg} secondary beam at 84 MeV per
nucleon. Measurements were made on both carbon and beryllium targets, having
very different structures, allowing a first investigation of the likely nature
of the pickup reaction mechanism. The measurements involve thick reaction
targets and -ray spectroscopy of the projectile-like reaction residue
for final-state resolution, that permit experiments with low incident beam
rates compared to traditional low-energy transfer reactions. From measured
longitudinal momentum distributions we show that the \nuc{12}{C}
(\nuc{22}{Mg},\nuc{23}{Mg}+\gamma)X reaction largely proceeds as a direct
two-body reaction, the neutron transfer producing bound \nuc{11}{C} target
residues. The corresponding reaction on the \nuc{9}{Be} target seems to largely
leave the \nuc{8}{Be} residual nucleus unbound at excitation energies high in
the continuum. We discuss the possible use of such fast-beam one-neutron pickup
reactions to track single-particle strength in exotic nuclei, and also their
expected sensitivity to neutron high- (intruder) states which are often
direct indicators of shell evolution and the disappearance of magic numbers in
the exotic regime.Comment: 8 pages, 5 figure
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