Immobilization of monocentric and polycentric types of anaerobic chytrid fungi in Ca-alginate

A procedure for the immobilization of rhizomycelia of two strains of anaerobic fungi, namely, Piromyces sp. KSX1 and Orpinomyces sp, 478P1 using Ca-alginate beads has been developed. Zoospores were found to be suitable propagules for the immobilization of the monocentric strain KSX1 giving rise to colonization in 100% of beads. For polycentric strain 478P1, which produced very few zoospores, partially homogenized rhizomycelia proved a suitable inoculum for the beads. The distribution of rhizomycelial growth inside the beads whereas strain KSZ1 grew in microcolonies throughout the bead and on the surface, and was unable to evenly colonize the entire Ca-alginate matrix

An MRI-histological study of white matter in stroke-free SHRSP

Hypertension is associated with cerebral small vessel disease (SVD) and with diffuse white matter hyperintensities (WMH) on T<sub>2</sub>-weighted magnetic resonance imaging (MRI). We tested whether stroke-prone spontaneously hypertensive rats (SHRSP), a model of chronic hypertension, exhibit WMH. Male SHRSP (age 10 months) without stroke symptoms were compared with age-matched male WKY rats. Stroke-prone spontaneously hypertensive rats exhibited no WMH on MRI scans (T<sub>2</sub>, T<sub>2</sub>*, diffusion tensor imaging) and no neuropathological lesions. While leptomeningeal arteries exhibited fibrohyaline wall thickening, with decreased smooth muscle actin relative to WKY, deep penetrating arterioles within the caudate nuclei had no vasculopathy. We conclude that WMH are not an obligate feature of stroke-free SHRSP aged up to 10 months