513 research outputs found

    Vascular Smooth Muscle Cells in Response to Gold Nanoparticles

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    In this master\u27s thesis we look at elucidating the interactions between nanoparticles and cells. Specifically, we looked at how the cell mechanics are affected, cytotoxicity of the nanoparticles, and shifts in cell phenotypes. There has been much research looking into whether nanoparticles are cytotoxic, but limited amounts looking at their effect on mechanics especially with vascular smooth muscle cells. This cell type has two distinct phenotypes of synthetic and contractile that each serve different purposes physiologically. The first experiments we did were cytotoxicity assays to see if the cells could survive the treatment with nanoparticles. If the cells died within a short period of time then we wouldn\u27t be able to take the next step and look at the mechanics of the cells. Most of the nanoparticles used proved to cause no change in proliferation rate of the cells; however, a couple did show some cytotoxic effects and were not used for further experimentation. Since the cells were surviving and proliferating after treatment with these nanoparticles we did atomic force microscopy to determine the elastic modulus of the cells that were treated with nanoparticles and those that were untreated. This allowed us to see if there was a significant increase or decrease caused by the nanoparticles. The results showed that there was a significant decrease in the elastic modulus of the cells treated with nanoparticles. Finally, we wanted to observe any possible phenotypic shifts in the cells by using immunofluorescence. The cells were stained for actin, microtubules (the main components of the cell\u27s cytoskeleton and thus mechanics), and nuclei. Vascular smooth muscle cells at low passage number in culture are typically in the contractile phase and this was proven with our images. The nanoparticle treated cells showed a shift towards the synthetic phenotype which confirmed the decrease in elastic modulus from the AFM data. So, while these nanoparticles are not cytotoxic we are causing a significant change in the cells\u27 mechanics and phenotype

    STUDIES OF VERY EARLY EVENTS OCCURRING IN BACTERIOPHAGE-SP82G INFECTION

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    An Analysis and Performance Guide of Olivier Messiaen’s Chants de Terre ET de Ciel

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    This dissertation presents an analysis of musical elements in Olivier Messiaen’s Chants de terre et de ciel in order to facilitate performance preparation of the work. To begin, general information about Messiaen’s religious and musical upbringing is introduced, as well as relevant details of his marriage to Claire Delbos and the birth of their son, Pascal. This is followed by a discussion of the poems which Messiaen set in the Chants and their surrealistic aspects. The musical analysis begins with an examination of Messiaen’s compositional techniques as outlined in Technique de mon langage musical, followed by discussions of those elements as they appear in the Chants. The songs are analyzed according to Messiaen’s modes of limited transposition, accords spéciaux, other means of pitch organization, and rhythmic organizations. Finally, the author presents practical guidance to aid performance preparation of the Chants. This guidance addresses several performance considerations particular to Messiaen and includes specific counting and coordination strategies to assist the pianist, singer, and ensemble. The analysis of the poetry, music, and performance considerations in this dissertation aims to support performers in developing an understanding of musical organization in the Chants and devising strategies for effective rehearsal of the work

    7-An Archaeological Survey of the Thornapple River Basin, Barry County Michigan: Phase One Completion Report

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    Archaeological Research in the Thornapple Drainage of Barry County The Project Area Previous Archaeological Research in Barry County Research Objectives for Phase One Assessment of Current Land Use Patterns Evaluation of Data in the State Site Files and Information Provided by Landowners/ Collectors and Local Institutions Limited Surface Reconnaissance in Selected Portions of the Basin and the County Description of Sites Recorded and Catalog of Surface Collections Interpretations and Conclusions Comments on Management of Cultural Resources and Future Research Needs Catalog of Artifactual Material from the Survey References Cited Thornapple Basin Survey, Barry County, Michigan Thornapple Basin Survey: Previously Recorded Archaeological Sites Thornapple Basin Survey: Land Use Survey Thornapple Basin Survey: Archaeological Site
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