A spectroscopic census of the M82 stellar cluster population

We present a spectroscopic study of the stellar cluster population of M82, the archetype starburst galaxy, based primarily on new Gemini-North multi-object spectroscopy of 49 star clusters. These observations constitute the largest to date spectroscopic dataset of extragalactic young clusters, giving virtually continuous coverage across the galaxy; we use these data to deduce information about the clusters as well as the M82 post-starburst disk and nuclear starburst environments. Spectroscopic age-dating places clusters in the nucleus and disk between (7, 15) and (30, 270) Myr, with distribution peaks at ~10 and ~140 Myr respectively. We find cluster radial velocities in the range (-160, 220) km/s (wrt the galaxy centre) and line of sight Na I D interstellar absorption line velocities in (-75, 200) km/s, in many cases entirely decoupled from the clusters. As the disk cluster radial velocities lie on the flat part of the galaxy rotation curve, we conclude that they comprise a regularly orbiting system. Our observations suggest that the largest part of the population was created as a result of the close encounter with M81 ~220 Myr ago. Clusters in the nucleus are found in solid body rotation on the bar. The possible detection of WR features in their spectra indicates that cluster formation continues in the central starburst zone. We also report the potential discovery of two old populous clusters in the halo of M82, aged >8 Gyr. Using these measurements and simple dynamical considerations, we derive a toy model for the invisible physical structure of the galaxy, and confirm the existence of two dominant spiral arms.Comment: Accepted for publication in the Astrophysical Journa

Kinetics and kinematics of diabetic foot in type 2 diabetes mellitus with and without peripheral neuropathy: a systematic review and meta-analysis.

Diabetes mellitus patients are at increased risk of developing diabetic foot with peripheral neuropathy, vascular and musculoskeletal complications. Problems develop with a relatively high risk of infection, gangrene and amputation. In addition, altered plantar pressure distribution is an important etiopathogenic risk factor for the development of foot ulcers. The purpose of this systematic review is to understand the biomechanical changes involved through studies of foot kinematic and kinetic in type 2 diabetes mellitus. Scientific articles were identified using electronic databases including Science Direct, CINAHL, Springer Link, Medline, Web of Science, and Pubmed. The selection of articles to include in the systematic review was narrorwed after reading the full text, focusing on studies that used experimental designs relating to the biomechanics of diabetic foot. The meta-analysis report on gait velocity (neuropathy = 128 and non-diabetes = 131) showed that there was a significantly lower gait velocity in neuropathy participants compared to non-diabetes age-matched participants at a high effect level (-0.09, 95% CI -0.13 to 0.05; p < 0.0001). Regarding knee-joint flexion range, there was a significant difference between neuropathy and non-diabetes groups (4.75, 95% CI, -7.53 to 1.97, p = 0.0008). The systematic review found significant differences in kinematic and kinetic variables among diabetic with neuropathy, diabetic without neuropathy and non-diabetic individuals. The review also found that the sample sizes used in some studies were not statistically significant enough contribute reliably to the meta-analysis, so further studies with higher sample sizes are required

Dust Properties of NGC4753

We report BVR surface photometry of a lenticular galaxy, NGC4753 with prominent dust lanes. We have used the multicolor broadband photometry to study dust-extinction as a function of wavelength and derived the extinction curve. We find the extinction curve of NGC 4753 to be similar to the Galactic extinction curve in the visible region which implies that the sizes of dust grains responsible for optical extinction are similar to those in our Galaxy. We derive dust mass from optical extinction as well as from the far infrared fluxes observed with IRAS. The ratio of the two dust masses, $\frac{M_{d,IRAS}}{M_{d,optical}}$, is 2.28 for NGC 4753, which is significantly lower than the value of 8.4 \pm 1.3 found previously for a large sample of elliptical galaxies. The total mass of the observed dust within NGC4753 is about a factor of 10 higher than the mass of dust expected from loss of mass from red giant stars and destruction by sputtering and grain-grain collisions in low velocity shocks, and sputtering in supernova driven blast waves. We find evidence for the coexistence of dust and H$\alpha$ emitting gas within NGC4753. The current star formation rate of NGC4753, averaged over past $2\times10^{6} yr$, is estimated to be less than 0.21M_{\sun}yr^{-1}. A substantial amount of dust within NGC4753 exists in the form of cirrus.Comment: 15 pages, 8 jpeg figures, 5 tables in one file, AASTEX style, Accepted for publication in the Astronomical Journal, 1999 Augus

"Then you get a teacher" - Guidelines for excellence in teaching

Background: Current literature calls for the explicit teaching to health-science educators of the skills, knowledge and dispositions that are required for successful teaching in higher education. Aims: This paper draws on evidence from an Oral Hygiene department at a South African university in order to illustrate these teaching-competency needs. Insights from the evidence are synthesised with current literature regarding best teaching practice, in support of an appropriate framework for the development of teaching competencies to health-science educators. Description: A qualitative approach, using a case study, was adopted. The cohort comprised fifteen students in the first-year Oral Hygiene cohort class and the ten educators who taught their programme. Data was collected through semistructured interviews and open-ended questionnaires. The topics that emerged from the combined analysis of the interviews and the questionnaires were organised into a grid so that common themes could be identified. Current literature regarding teaching and learning was used as a framework for interpreting the empirical evidence, from which three categories emerged. The first category included suggestions from students regarding what to do to teach better. A review of the literature indicates that these competencies can be effectively learnt from self-help guides. The second category included requests for skills development. Literature review suggests that these might effectively be learnt from single-event workshops facilitated by more able peers. Responses in the final category highlighted the need for an underpinning theory of teaching and learning, and signalled the need for a more theoretically grounded and detailed approach to teacher development. Conclusion: The framework developed from the empirical study and current literature makes it possible for individual clinical teachers, and staff developers, to construct teaching-competency development plans that are pertinent to individual teachers’ needs, relevant and practical, educationally sound, and cost-effective in terms of time and effort

An evaluation of thoron (and radon) equilibrium factorclose towalls based on long-term measurements in dwellings

Thoron gas and its progeny behave quite differently in room environments, owing to the difference in their half-lives; therefore, it is important to measure simultaneously gas and progeny concentrations to estimate the time-integrated equilibrium factor. Furthermore, thoron concentration strongly depends on the distance from the source, i.e. generally walls in indoor environments. In the present work, therefore, the measurements of both thoron and radon gas and their progeny concentrations were consistently carried out close to the walls, in 43 dwellings located in the Sokobanja municipality, Serbia. Three different types of instruments have been used in the present survey to measure the time-integrated thoron and radon gas and their progeny concentrations simultaneously. The equilibrium factor for thoron measured ‘close to the wall’, FW Tn, ranged from 0.001 to 0.077 with a geometric mean (GM) [geometric standard deviation (GSD)] of 0.006 (2.2), whereas the equilibrium factor for radon, FRn, ranged from 0.06 to 0.95 with a GM (GSD) of 0.23 (2.0)

T cell cytolytic capacity is independent of initial stimulation strength.

How cells respond to myriad stimuli with finite signaling machinery is central to immunology. In naive T cells, the inherent effect of ligand strength on activation pathways and endpoints has remained controversial, confounded by environmental fluctuations and intercellular variability within populations. Here we studied how ligand potency affected the activation of CD8+ T cells in vitro, through the use of genome-wide RNA, multi-dimensional protein and functional measurements in single cells. Our data revealed that strong ligands drove more efficient and uniform activation than did weak ligands, but all activated cells were fully cytolytic. Notably, activation followed the same transcriptional pathways regardless of ligand potency. Thus, stimulation strength did not intrinsically dictate the T cell-activation route or phenotype; instead, it controlled how rapidly and simultaneously the cells initiated activation, allowing limited machinery to elicit wide-ranging responses

Identification of Phosphoproteins as Possible Differentiation Markers in All-Trans-Retinoic Acid-Treated Neuroblastoma Cells

BACKGROUND: Neuroblastic tumors account for 9-10% of pediatric tumors and neuroblastoma (NB) is the first cause of death in pre-school age children. NB is classified in four stages, depending on the extent of spreading. A fifth type of NB, so-called stage 4S (S for special), includes patients with metastatic tumors but with an overall survival that approximates 75% at five years. In most of these cases, the tumor regresses spontaneously and regression is probably associated with delayed neuroblast cell differentiation. METHODOLOGY/PRINCIPAL FINDINGS: In order to identify new early markers to follow and predict this process for diagnostic and therapeutics intents, we mimicked the differentiation process treating NB cell line SJ-NK-P with all-trans-retinoic acid (ATRA) at different times; therefore the cell proteomic pattern by mass spectrometry and the phosphoproteomic pattern by a 2-DE approach coupled with anti-phosphoserine and anti-phosphotyrosine western blotting were studied. CONCLUSIONS/SIGNIFICANCE: Proteomic analysis identified only two proteins whose expression was significantly different in treated cells versus control cells: nucleoside diphosphate kinase A (NDKA) and reticulocalbin-1 (RCN1), which were both downregulated after 9 days of ATRA treatment. However, phosphoproteomic analysis identified 8 proteins that were differentially serine-phosphorylated and 3 that were differentially tyrosine-phosphorylated after ATRA treatment. All proteins were significantly regulated (at least 0.5-fold down-regulated). Our results suggest that differentially phosphorylated proteins could be considered as more promising markers of differentiation for NB than differentially expressed proteins