Détection directionnelle de matière sombre avec MIMAC

De nombreuses mesures cosmologiques et astrophysiques tendent à montrer que notre galaxie serait englobée par un halo de matière sombre non-baryonique. La détection directionnelle vise à mesurer la direction du recul nucléaire issu d'une interaction avec une particule de matière sombre. Cela permettrait de mettre en évidence la forte dépendance angulaire de la distribution de reculs due à la rotation du système solaire autour du centre galactique. Cette thèse aborde la détection directionnelle par une approche multi-thématique : phénoménologie, expérimentale et analyse de données. L'objectif des études phénoménologiques est de montrer l'apport d'un détecteur directionnel en terme de recherche de matière sombre. Grâce au développement de méthodes statistiques dédiées, on montre qu'un détecteur tel que celui proposé par la collaboration MIMAC, devrait permettre de découvrir la matière sombre avec une grande significance jusqu'à des sections efficaces 2 à 3 ordres de grandeur en dessous des limites actuelles. La mise en place d'une méthodologie d'analyse de données directionnelles constitue un second objectif de cette thèse car la reconstruction 3D des traces mesurées est un point clef de cette nouvelle stratégie de détection. On présente ainsi une nouvelle méthode d'analyse basée sur une approche par vraisemblance, permettant d'optimiser l'estimation des paramètres de chaque événement mesuré: position dans le détecteur et direction. Dans le cadre de la discrimination du bruit de fond électronique, on a mis en place une étude basée sur la topologie de la trace et utilisant une analyse par arbres de décision boostés qui nous permet d'obtenir des facteurs de rejet environ 20 fois supérieurs à ceux obtenus avec des analyses séquentielles. Du point de vue expérimental, on présente une méthode originale de la mesure de vitesse de dérive des électrons permettant d'obtenir des incertitudes de l'ordre du pourcent et de contraindre simultanément les coefficients de diffusion longitudinale. On termine enfin sur l'analyse des données obtenues auprès du champ de neutrons AMANDE permettant de valider la stratégie de détection du projet MIMAC.A large number of cosmological and astrophysical measurements supports the fact that our galaxy should be immersed in a halo of non-baryonic dark matter. Directional detection aims at measuring the direction of recoiling nucleus following an elastic scattering with a Dark Matter particle. This should allow us to show the expected strong angular dependence of the recoil distribution due to the rotation of the Solar System around the galactic center. This thesis presents a comprehensive study of directional detection following three different aspects: phenomenology, experimental and data analysis. The goal of the phenomenological studies is to explore the interest of directional detection in terms of galactic dark matter search. With the use of dedicated statistical tools we show that a detector, as the one proposed by the MIMAC collaboration, should be able to discover dark matter with a high significance down to cross sections 2 to 3 orders of magnitude below current limits. Setting up a new strategy of data analysis is a second goal of this thesis as an efficient 3D track reconstruction is compulsory to achieve an accurate directional detection of dark matter. We present a new method based on a likelihood approach aiming at the optimisation of the estimation of the parameters of each measured track: position in the detector volume and direction. In the context of reducing the electronic background, we present a method based on the analysis of the track topology using a boosted decision tree algorithm which enhances the rejection power by a factor 20 with respect to a sequential analysis. In the context of experimental measurement, we present a new method dedicated to the measurement of the electron drift velocity with uncertainties of the order of the percent and constraining the longitudinal diffusion coefficients. Eventually, we discuss the results of a data analysis obtained during an acquisition using a neutron field which validate the detection strategy of the MIMAC experiment.SAVOIE-SCD - Bib.électronique (730659901) / SudocGRENOBLE1/INP-Bib.électronique (384210012) / SudocGRENOBLE2/3-Bib.électronique (384219901) / SudocSudocFranceF

Huntingtin proteolysis releases non-polyQ fragments that cause toxicity through dynamin 1 dysregulation

Cleavage of mutant huntingtin (HTT) is an essential process in Huntington's disease (HD), an inherited neurodegenerative disorder. Cleavage generates N-ter fragments that contain the polyQ stretch and whose nuclear toxicity is well established. However, the functional defects induced by cleavage of full-length HTT remain elusive. Moreover, the contribution of non-polyQ C-terminal fragments is unknown. Using time- and site-specific control of full-length HTT proteolysis, we show that specific cleavages are required to disrupt intramolecular interactions within HTT and to cause toxicity in cells and flies. Surprisingly, in addition to the canonical pathogenic N-ter fragments, the C-ter fragments generated, that do not contain the polyQ stretch, induced toxicity via dilation of the endoplasmic reticulum (ER) and increased ER stress. C-ter HTT bound to dynamin 1 and subsequently impaired its activity at ER membranes. Our findings support a role for HTT on dynamin 1 function and ER homoeostasis. Proteolysis-induced alteration of this function may be relevant to disease. Synopsis The development of a time and site-specifically controlled cleavage of the mutant huntingtin protein reveals a pathogenic mechanism induced by the non-polyQ-containing fragments that are generated upon proteolysis during disease progression. Huntingtin proteolysis generates N-ter fragments that contain the toxic polyQ stretch but also the corresponding C-ter fragments. N-ter to C-ter intramolecular interactions present in full-length huntingtin are abrogated by sequential cleavages. Whereas the N-ter polyQ fragments translocate into the nucleus, the non-polyQ C-ter huntingtin fragments remain in the cytoplasm and cause ER dilation, stress and cell death. C-ter huntingtin fragments bind and inactivate dynamin 1 at the ER thus causing ER dilation and toxicity. Site-specifically controlled cleavage of the mutant huntingtin protein reveals a pathogenic mechanism induced by non-polyQ-containing fragments that are generated upon proteolysis during disease progression.</p

Differential Impact According to Mission’s Operational Intensity on Psychoactive Substance Use: A Retrospective Cohort of French Male Army Service Members

International audienceBackground: Stressful deployments in combat areas are known to increase the risk of substance abuse in military personnel. Objectives: The aim of the study was to compare deployment on stressful, high-intensity missions (HIMs) to deployment on low-intensity missions (LIMs) in order to understand factors associated with substance use variations across the mission. Methods: A retrospective cohort study based on a one-shot self-questionnaire was performed four months after their return on two samples of male French Army service members: one returning from an HIM and one from an LIM. The questionnaire focused on tobacco, alcohol, cannabis, cocaine, and psychoactive medication use at three times: before, during, and after the mission. Results: During an HIM, the frequency of tobacco use increased, alcohol use remained stable – although 38% declared a decrease in consumption – and illicit drug use decreased. During an LIM, tobacco and alcohol use increased, cannabis use remained stable, and only cocaine and medication use decreased. After their return, use levels among both samples reverted to values similar to those reported before the mission, except for a decrease in tobacco use observed at return from an HIM. The main factors perceived as related to variations were stress in an HIM and low cost in an LIM. Conclusions/importance: The study suggests a differential impact of deployment on substance use according to the operational intensity of the mission. Variations in use are predominant during the mission with a washout effect after returning home

Gamma-ray spectroscopy for the characterization of uranium contamination in nuclear decommissioning

International audienceDecommissioning is the last step in the life cycle of a nuclear facility. After the evacuation of the facility components, the remaining structures such as concrete walls and floors must be surveyed to ensure that no residual contamination remains. It is a costly and time consuming activity, for which CEA develops fast alpha and beta detection methods allowing a full scanning of very large areas (hundreds of thousands of square meters) in legacy uranium enrichment plants. To support these developments, we present here complementary high-resolution gamma-ray spectroscopy analyses of a contaminated area at the gaseous diffusion uranium enrichment facility UDG, currently under decommissioning at Pierrelatte nuclear plant, France. Long measurements are performed with a High-Purity Germanium (HPGe) detector on the contaminated surface, and in a clean area to assess the natural gamma background of the concrete ground. The surface activity of uranium is 16.6 ± 6.0 Bq.cm-2, mainly due to 234U and 238U, most of the uncertainty coming from the non-uniform distribution of the contamination on the ground. This measurements also allowed us estimating the uranium enrichment of the contamination, which amounts to (0.80 ± 0.13) % of 235U mass fraction, consistently with the range of the Low Enrichment Plant where this measure was performed. Eventually, the background spectrum allowed us to determine the mass fractions of natural uranium, thorium and potassium in the concrete ground, which respectively amount to 3.8 ± 0.2. ppmU (i.e. 3.8 mg of uranium per kg of concrete), 7.4 ± 0.7 ppmTh, and (2.6 ± 0.1) %K of potassium

PANCO2: A new software to measure pressure profiles from resolved thermal SZ observations

International audienceWe have developed a new software to perform the measurement of galaxy cluster pressure profiles from high angular resolution thermal SZ observations. The code allows the user to take into account various features of millimeter observations, such as point spread function (PSF) convolution, pipeline filtering, correlated residual noise, and point source contamination, in a forward modeling approach. A major advantage of this software is its performance, enabling the extraction of the pressure profile and associated confidence intervals via MCMC sampling in times as short as a few minutes. We present the code and its validation on various realistic synthetic maps, of ideal spherical clusters, as well as of realistic, hydrodynamically simulated objects. We plan to publicly release the software in the coming months

Forecasting the Y500 – M500 scaling relation from the NIKA2 SZ Large Program

International audienceOne of the key elements needed to perform the cosmological exploitation of a cluster survey is the relation between the survey observable and the cluster masses. Among these observables, the integrated Compton parameter Y is a measurable quantity in Sunyaev-Zeldovich (SZ) surveys, which tightly correlates with cluster mass. The calibration of the relation between the Compton parameter Y500 and the mass M500 enclosed within radius R500 is one of the scientific goals of the NIKA2 SZ Large Program (LPSZ). We present an ongoing study to forecast the constraining power of this program, using mock simulated datasets that mimic the large program sample, selection function, and typical uncertainties on Y500 and M500. We use a Bayesian hierarchical modelling that enables taking into account a large panel of systematic effects. Our results show that the LPSZ can yield unbiased estimates of the scaling relation parameters for realistic input parameter values. The relative uncertainties on these parameters is ~ 10% for the intercept and slope of the scaling relation, and ~ 34% for its intrinsic scatter, foreshadowing precise estimates to be delivered by the LPSZ

Impact of Tobacco Smoking on the Risk of COVID-19: A Large Scale Retrospective Cohort Study

International audienceIntroduction: Preliminary reports indicated that smokers could be less susceptible to coronavirus SARS-CoV-2, which causes Covid-19. However, once infected an increased risk of severe disease is reported. We investigated the association between smoking and COVID-19 during an outbreak of the disease on a naval vessel.Methods: We conducted a cross-sectional, observational study on the 1769 sailors of the same navy aircraft carrier at sea exposed at the same time to SARS-CoV2 to investigate the link between tobacco consumption and Covid-19.Results: Among the 1688 crewmembers (87% men; median age = 28 [interquartile range 23-35]) included, 1279 (76%) developed Covid-19 (1038 [62%] reverse-transcriptase- polymerase chain reaction testing-positive and 241 [14%] with only clinical signs). One hundred and seven patients were hospitalized. The univariable analysis odds ratio (OR) for Covid-19 infection was 0.59 (95% confidence interval [CI], 0.45-0.78; p 50 years old had an increased risk of contracting Covid-19 (OR, 2.84 [95% CI, 1.30-7.5]; p = .01). Multivariable analysis retained the lower risk of current smokers becoming infected (OR, 0.64 [0.49-0.84]; p 50 years was significatively associated with Covid-19 (OR, 2.6 [1.17-6.9]; p = .03).Conclusions: Current smoking status was associated with a lower risk of developing Covid-19 but cannot be considered as efficient protection against infection. The mechanism of the lower susceptibility of smokers to SARS-CoV-2 requires further research.Trial registration: IRB no.: 0011873-2020-09.Implications: (1) Recent epidemiologic data suggest a paradoxical link between smoking and COVID-19. (2) Among the 1688 crewmembers (with an attack rate of 76% and exposed at the same time in the same place to SARS-CoV2), we found a significantly lower risk for developing COVID-19 in current smokers (71%) versus former and nonsmokers (80%). This finding strongly supports the need for further research on nicotine physiological pathway and its impact on COVID-19 infection whilst emphasizing that tobacco smoking should not be considered as efficient protection against COVID-19

The case for an all-sky millimetre survey at subarcminute resolution

International audienceThere are several new projects to survey the sky with millimetre eyes, the biggest being Simons Observatory and CMB-S4, in the Southern Hemisphere. The NIKA2 collaboration has acquired sufficient knowledge to build a large focal plane KID camera for a 15 m antenna. This would allow covering the whole Northern Hemisphere in five years at subarcminute resolution and with milliJansky point-source sensitivity. We describe the main scientific drivers for such a project: the SZ sky, the high-redshift millimetre Universe and the interstellar medium in our Galaxy and the nearby galaxies. We also show briefly the main difficulties (scientific, organisational, technical and financial)