A laser tracking dynamic robot metrology instrument

Research work over several years has resulted in the development of a laser tracking instrument capable of dynamic 3-D measurements of robot end-effector trajectories. The instrument characteristics and experiments to measure the static and dynamic performance of a robot in an industrial manufacturing environment are described. The use of this technology for space applications is examined

Detection of HIV-1 infection in dried blood spots from a 12-year-old ABO bedside test card

Background and Objectives: We tested dried blood from an ABO bedside test card which had been stored at room temperature for 12 years, to prove that a patient with HIV-1 infection had been infected by blood transfusion. Materials and Methods: Immunoblots for HIV-1 antibodies and threefold PCRs with half-nested primers for the HIV-1 integrase gene were done with eluates from the dried blood spots. Results: HIV-1 antibodies and HIV-1 DNA could be detected in the sample from one unit of blood, but not from the two other units or from the recipient before transfusion. Conclusion: Further studies should be done on the validity of stored dried blood as an alternative to the storage of frozen donor serum for several years for `look-back' studies

Potential of different composts to improve soil fertility

Composts can influence soil fertility and plant health. These influences can be positive or negative, depending of the quality of the composts. Some practitioners already make use of the positive effects on plant health. For example, they use composts to protect their plants against soil borne diseases in substrate, or to detoxify and reactivate soil after steaming. In order to estimate the potential of Swiss composts to influence soil fertility and plant health positively, we analyzed one hundred composts representative of the different composting systems and qualities available on the market. The organic substance and the nutrient content of the composts varied greatly between the composts; the materials of origin were the major factor influencing these values. The respiration rate and enzyme activities also varied greatly, particularly in the youngest composts. These differences become smaller when the composts become more mature. Maturity, the degradation stage of the organic matter, depended not only on the age of the compost, but also on the management of the process. The N-mineralization potential from compost added to soil showed that a high proportion of young composts immobilized the nitrogen in the soil. This problem was hardly correlated with the materials of origin, but with the management of the first stage of the composting process. Especially composts which had become too dry in this period lost their ammonia-nitrogen, and hence immobilized nitrogen in the soil. Also composts with a low NO3/NH4 ratio, as a rough indicator for an immature compost, immobilized nitrogen in the soil. By contrast, the phytotoxicity of the composts varied very much also in matured composts, showing that the storage of the compost plays a decisive role. While the majority of compost protected cucumber plants against Pythium ultimum, only a few composts suppressed Rhizoctonia solani in basil. With respect to disease suppression, the management of the maturation process seems to play a major role. In conclusion, big differences in compost quality and of their impact on soil fertility and on plant health were observed. The management of the composting process seems to influence the quality of the composts to a higher extent than the materials of origin or the composting system. More attention should be paid to biological quality of composts, in order to produce composts with more beneficial effects on crops

Part I: A novel in-vitro system for simultaneous mechanical stimulation and time-lapse microscopy in 3D

To investigate the migration response of cells to changes in their biophysical environment, a novel uniaxial cell stimulation device (UCSD) has been designed and tested. The device is capable of applying very precise user-defined static or dynamic mechanical stimuli in a physiologically relevant strain window (up to 50%) and frequency bandwidth (up to 2Hz) to cells residing in a three-dimensional (3D) environment while single-cell migration is simultaneously measured by time-lapse microscopy. The system is an advancement over uniaxial loading devices reported to date in that it allows temporal and spatial quantification of migration as a function of the micromechanical environment. We make use of the favorable physical and biological properties of poly(ethylene glycol) hydrogels as model matrix and present a method for fabricating cell-containing hydrogel constructs. The 3D strain field within these constructs is modeled by finite element analysis. Fibroblasts reversibly altered their morphology and orientation in response to the strain field. In the succeeding companion paper we then exploit the system to analyze fibroblast motility induced by different stimulation regimes (refer to part II

Focused-ion-beam milling based nanostencil mask fabrication for spin transfer torque studies

Focused-ion-beam milling is used to fabricate nanostencil masks suitable for the fabrication of magnetic nanostructures relevant for spin transfer torque studies. Nanostencil masks are used to define the device dimensions prior to the growth of the thin film stack. They consist of a wet etch resistant top layer and an insulator on top of a pre-patterned bottom electrode. The insulator supports a hard mask and gives rise to an undercut by its selective etching. The approach is demonstrated by fabricating current perpendicular to the plane Co/Cu/Co nanopillar junctions, which exhibit current-induced magnetization dynamics.Comment: 13 pages, 3 figures, submitted to AP

1995, Spatial and temporal variability of late Neogene equatorial Pacific carbonate

High-resolution, continuous records of GRAPE wet bulk density (a carbonate proxy) from Ocean Drilling Program Leg 138 provide one the opportunity for a detailed study of eastern equatorial Pacific Ocean carbonate sedimentation during the last 6 m.y. The transect of sites drilled spans both latitude and longitude in the eastern equatorial Pacific from 90° to 110°W and from 5°S to 10°N. Two modes of variability are resolved through the use of Empirical Orthogonal Function (EOF) analysis. In the presence of large tectonic and climatic boundary condition changes over the last 6 m.y., the dominant mode of spatial variability in carbonate sedimentation is remarkably constant. The first mode accounts for over 50% of the variance in the data, and is consistent with forcing by equatorial divergence. This mode characterizes both carbonate concentration and carbonate mass accumulation rate time series. Variability in the first mode is highly coherent with insolation, indicating a strong linear relationship between equatorial Pacific car bonate sedimentation and Milankovitch variability. Frequency domain analysis indicates that the coupling to equatorial divergence in carbonate sedimentation is strongest in the precession band (19-23 k.y.) and weakest though present at lower frequencies. The second mode of variability has a consistent spatial pattern of east-west asymmetry over the past 4 m.y. only; prior to 4 Ma, a different mode of spatial variability may have been present, possibly suggesting influence by closure of the Isthmus of Panama or other tectonic changes. The second mode of variability may indicate influence by CaCO3 dissolution. The second mode of variability is not highly coherent with insolation. Comparison of the modes of carbonate variability to a 4 m.y. record of benthic δ 1 8 indicates that although overall correlation between carbonate and δ 1 8 is low, both modes of variability in carbonate sedimentation are coherent with δ 1 8 changes at some frequencies. The first mode of carbonate variability is coherent with Sites 846/849 δ 1 8 at the dominant insolation periods, and the second mode is coherent at 100 k.y. during the last 2 m.y. The coherence between carbonate sedimentation and δ 1 8 in both EOF modes suggests that multiple uncorrelated modes of variability operated within the climate system during the late Neogene

Polarization-selective excitation of N-V centers in diamond

The nitrogen-vacancy (N-V) center in diamond is promising as an electron spin qubit due to its long-lived coherence and optical addressability. The ground state is a spin triplet with two levels ($m_s = \pm 1$) degenerate at zero magnetic field. Polarization-selective microwave excitation is an attractive method to address the spin transitions independently, since this allows operation down to zero magnetic field. Using a resonator designed to produce circularly polarized microwaves, we have investigated the polarization selection rules of the N-V center. We first apply this technique to N-V ensembles in [100] and [111]-oriented samples. Next, we demonstrate an imaging technique, based on optical polarization dependence, that allows rapid identification of the orientations of many single N-V centers. Finally, we test the microwave polarization selection rules of individual N-V centers of known orientation