2,580 research outputs found

    1.5 to Stay Alive: How We Find Hope and Honesty in Dangerous Times

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    Roles of Peroxisomes and Peroxisome-Derived Products in Controlling Plant Growth and Stress Responses

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    The peroxisome is a vital organelle conserved through the entire eukaryotic lineage. In all examined species, peroxisomes are responsible for such essential processes as fatty acid beta-oxidation and metabolism of reactive oxygen species (ROS). In plants, peroxisomes have taken on additional specialized roles, such as production of some plant hormones and vitamins. In this work, I have uncovered novel factors regulating peroxisome number in model species Arabidopsis thaliana, and novel mechanisms governing how peroxisomes respond to salt stress. I discovered a role for Arabidopsis MAP KINASE17 (MPK17) as a negative regulator of peroxisome division that acts in the salt-stress response pathway of peroxisome division. Additionally, I uncovered a novel role for the known peroxisome division factor PEROXISOME AND MITOCHONDRIAL DIVISION FACTOR 1 (PMD1) as another regulator of salt-induced peroxisome division and as the first known plant peroxisome division factor to bind to actin. A forward genetics approach was undertaken to attempt to isolate peroxisome-deficient mutants in the genetically tractable crop species Solanum lycopersicum, screening mutagenized tomato seeds on the auxin precursor indole-3-butyric acid (IBA). Although no peroxisome mutants were isolated by this method, several mutants impaired in various other aspects of auxin homeostasis were isolated and used to make new discoveries regarding the contributions of IBA to vegetative and reproductive tomato development

    Health and the nature of urban green spaces

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    Recognition that city-dwelling people can benefit from contact with nature is not new. The urban open air movement of the 19th century advised retention of greenways and development of urban parks and gardens to stop disease spread through lack of fresh air, poor sanitation and overcrowding. Now, in the early 21st century, urban green spaces are under threat from inner city infill projects and clearing of remnant vegetation to accommodate suburban sprawl. While much literature discusses positive health benefits of contact with nature, few studies explore explicit pathways between urban green space and health, despite mounting concern that disassociation between people and nature in urban communities may be detrimental to physical and mental health. This study explored how people’s attitudes toward nature might influence perceptions of nearby green spaces and feelings of attachment to living in their neighbourhood, and in turn, whether people with positive attitudes towards nature and positive perceptions of nearby green space would report better health. A mixed method research design was adopted in this study. Exploration of research questions required objective measurement of relationships between different aspects of health and nature, and interpretation of the subjective meanings people attach to those relationships. Study design involved distribution of a cross-sectional survey to residents in four neighbourhoods in Perth, Western Australia with respondents invited to participate in a semi-structured interview. Neighbourhoods were selected based on location (either an inner or outer suburban area), age of neighbourhood (established or new), diversity of nearby green space, and socio-demographic characteristics. Data from 440 surveys and 25 interviews were analysed. Attitudes towards urban nature were diverse and it was clear that feelings about natural environments strongly influenced preference and perceptions of useable green spaces, and for some people, their choice of neighbourhood. In essence, people who enjoyed spending time in nature were more inclined to seek green spaces within their neighbourhood environment that provided complexity and opportunities for exploration or escape. Those who professed little connection to nature and saw bushland areas as untidy, uninviting or unsafe, tended to be more concerned about aesthetic and functional aspects of green space design and preferred to visit ‘civilised’ parks and gardens with manicured lawns, formal paths and playgrounds. Green spaces were important sites for physical activity, relaxation and social interaction and proximity to useable green space was a significant factor in predicting better selfreported health. In addition, neighbourhoods with trees and greenways were described as healthier places to live. People who lived in close proximity to parks and green spaces where social interaction regularly occurred, who reported that diverse green spaces and bushland areas were being retained in their neighbourhood, who cared about environmental issues and were interested in being involved in conservation activities, were more likely to report better physical function, general health, mental health and feelings of vitality. People who regularly visited nearby green spaces described feeling happier and more satisfied with living in their neighbourhood. Encouraging people to regularly visit and become actively involved in caring for local nature reserves and parklands can play an important role in health promotion and preventive health strategies. Conservation, useability and management of diverse green spaces must be considered as a critical element of urban planning. This will only occur with continuing recognition of the health benefits that can be achieved by retaining diverse, quality green spaces within suburban neighbourhoods

    An Investigation of Diabetes Mellitus in Postmortem Human Remains

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    Diabetes mellitus is one of the most prevalent and significant metabolic diseases impacting modern human populations. The goal of this research is to explore several analytical methods to better appreciate how diabetes impacts the skeleton, and to determine if this effect can be recognized in postmortem remains. Anthropologists are tasked with elucidating the relationship between nutrition, metabolism, growth, development, and skeletal health. Diabetes represents a crucial point of interface between these factors. Furthermore, as the percentage of diabetics increases in the general population, so will their representation in forensic cases. This study will provide tools for identifying characteristics of diabetes in the postmortem material available to anthropologists. Diabetes is a disease process that can alter the function of many tissues and systems. For these reasons, three analytical approaches were conducted including: blood serum protein analysis using ELISA, bone mineral density (BMD) scans with a dual-energy x-ray absorptiometry (DXA) scanner, and macroscopic osteological analysis. This study was completed employing a sample of 80 known skeletal donations and 20 blood samples from the William M. Bass Donated Skeletal Collection at the University of Tennessee, Knoxville. Results indicated that pro-inflammatory biomarkers may be quantified in postmortem blood samples, and that diabetics showed slightly higher average concentrations of cytokines associated with diabetes and lower concentrations of those related to insulin sensitivity. Bone density analysis revealed that diabetics and non-diabetics significantly differ in BMD, but this relationship varies between the sexes. Female diabetics had consistently denser bones in all measured variables of the lower limb, and one-third of forearm variables. Results based on male data did not display a similar outcome, with little difference observed between male diabetics and non-diabetics. Analysis of skeletal pathologies identified a set of three osteological variables, concentrated in the feet, as having the highest discriminatory potential. An accuracy rate of 83% was achieved in classifying individuals into diabetic versus non-diabetic categories

    The spores of Lycopodium, Phylloglossium, Selaginella and Isoetes and their value in the study of microfossils of Palaeozoic age

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    The LYCOPODIINAE are a ubiquitous race of plants: members are found in many different parts of the world from the tropics to the polar regions, and tinder a great variety of habitat conditions.The families with living representatives afford ample evidence of antiquity; and their fossil relatives indicate the abundance of the group during Palaeozoic times. The coals of the Carboniferous Age are to a large extent composed of the carbonised remains of these plants; their spores are found in vast numbers in most coal seams.The study of fossil spores within the last 25 years has led to their being used as an aid in the correlation of coal seams. Up to the present, the classification of fossil spores has been an arbitrary one; with the increasing use of spores as zonal indices in coalfield work, a more scientific system of nomenclature has been found necessary. A binomial system has been promulgated in America which in all probability will form the basis of future work on classification. Although a general review of the spores of modern Pteridophyta has already been published by the author (Knox, 1938), the lack of any comprehensive survey of the spore morphology of living species of Vascular Cryptogams has proved a considerable handicap in framing a classification of fossil spores; the present study of the spores of modern species of the LYCOPODIINAE has been undertaken with a view to gaining more exact knowledge of spore morphology in this large and important group, both generic and specific, in order to make possible an assessment of relative values for purposes of palaeobotanical taxonomy.The basis of the following work is furnished by a detailed account of the morphology of the spores of Lycopodium, Phylloglossum, Selaginella and Isoetes, derived from as many of the living species as it has been possible to obtain samples
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