LIVER BIOPSY: IMPORTANCE OF SPECIMEN SIZE IN THE DIAGNOSIS AND STAGING OF CHRONIC VIRAL HEPATITIS

Liver biopsy is the gold standard method for the grading and staging of chronic viral hepatitis, but optimal biopsy specimen size remains controversial. The aim of this study was to evaluate the quality of liver specimen (number of portal tracts) and to evaluate the impact of the number of portal tracts in the staging of chronic hepatitis. Material and Methods: 468 liver biopsies from consecutive patients with hepatitis C virus and hepatitis B virus infection from 2009 to 2010 were evaluated. Results: The length of fragment was less than 10 mm in 43 cases (9.3%), between 10 and 14 mm in 114 (24.3%), and ≥ 15 mm in 311 (64.4%); of these, in 39 (8.3%) cases were ≥ 20 mm. The mean representation of portal tracts was 17.6 ± 2.1 (5-40); in specimens ≥ 15 mm the mean portal tract was 13.5 ± 4.7 and in cases ≤ 15 mm was 11.4 ± 5.0 (p = 0.002). Cases with less than 11 portal tracts were associated with F3, and cases with 11 or more portal tracts with F2 (p = 0.001). Conclusion: this study demonstrated the good quality of liver biopsy and a relationship between the macroscopic size of the fragment and the number of portal tracts

Decision-making framework for implementing safer human-robot collaboration workstations: system dynamics modeling

Human-Robot Collaboration (HRC) systems are often implemented seeking for reducing risk of Work-related Musculoskeletal Disorders (WMSD) development and increasing productivity. The challenge is to successfully implement an industrial HRC to manage those factors, considering that non-linear behaviors of complex systems can produce counterintuitive effects. Therefore, the aim of this study was to design a decision-making framework considering the key ergonomic methods and using a computational model for simulations. It considered the main systemic influences when implementing a collaborative robot (cobot) into a production system and simulated scenarios of productivity and WMSD risk. In order to verify whether the computational model for simulating scenarios would be useful in the framework, a case study in a manual assembly workstation was conducted. The results show that both cycle time and WMSD risk depend on the Level of Collaboration (LoC). The proposed framework helps deciding which cobot to implement in a context of industrial assembly process. System dynamics were used to understand the actual behavior of all factors and to predict scenarios. Finally, the framework presented a clear roadmap for the future development of an industrial HRC system, drastically reducing risk management in decision-making.This work was supported by European Structural and Investment Funds in the FEDER component, through the Operational Competitiveness and Internationalization Programme (COMPETE 2020) [Project n◦ 39479; Funding Reference: POCI-01-0247-FEDER-39479] and by FCT - Fundação para a Ciência e Tecnologia within the R&D Units Project Scope: UIDB/00319/202

Ambiente urbano como promotor da saúde: aplicação do Índice de Bem-Estar Urbano na cidade de Conchal-SP, Brasil

Este artigo visa apresentar o processo de aplicação do Índice de Bem-Estar Urbano (IBEU) realizado em 2014, no município de Conchal-São Paulo, Brasil. Destaca a importância de estudos quantitativos para futuras o desenvolvimento de politicas publicas a serem adotadas no local, que vise alcançar um bem-estar diferencial da população urbana. Os dados utilizados para o estudos foram os do Censo Demográfico Brasileiro (IBGE, 2010). A metodologia e a criação do IBEU foram elaboradas pelo Observatório das Metrópoles, diferindo-se nesse estudo pela aplicação do índice ser em um município de pequeno porte com 25.229 habitantes. Os cinco indicadores utilizados são: mobilidade urbana, condições ambientais urbanas, condições habitacionais urbanas, atendimento de serviços coletivos urbanos e infraestrutura urbana. Ao final do estudo identificou-se que o Índice de Bem-Estar de Conchal foi de 0.863, que é considerado de bom para ótimo. Este estudo foi vinculado à disciplina Ambiente Urbano como Promotor da Saúde da FEC/UNICAMP, ao LABINUR e ao GEPUCS5816417

Comparative analysis of two complete Corynebacterium ulcerans genomes and detection of candidate virulence factors

Trost E, Al-Dilaimi A, Papavasiliou P, et al. Comparative analysis of two complete Corynebacterium ulcerans genomes and detection of candidate virulence factors. BMC Genomics. 2011;12(1): 383.ABSTRACT: Corynebacterium ulcerans has been detected as a commensal in domestic and wild animals that may serve as reservoirs for zoonotic infections. During the last decade, the frequency and severity of human infections associated with C. ulcerans appear to be increasing in various countries. As the knowledge of genes contributing to the virulence of this bacterium was very limited, the complete genome sequences of two C. ulcerans strains detected in the metropolitan area of Rio de Janeiro were determined and characterized by comparative genomics: C. ulcerans 809 was initially isolated from an elderly woman with fatal pulmonary infection and C. ulcerans BR-AD22 was recovered from a nasal sample of an asymptomatic dog. The circular chromosome of C. ulcerans 809 has a total size of 2,502,095 bp and encodes 2,182 predicted proteins, whereas the genome of C. ulcerans BR-AD22 is 104,279 bp larger and comprises 2,338 protein-coding regions. The minor difference in size of the two genomes is mainly caused by additional prophage-like elements in the C. ulcerans BR-AD22 chromosome. Both genomes show a highly similar order of orthologous coding regions; and both strains share a common set of 2,076 genes, demonstrating their very close relationship. A screening for prominent virulence factors revealed the presence of phospholipase D (Pld), neuraminidase H (NanH), endoglycosidase E (EndoE), and subunits of adhesive pili of the SpaDEF type that are encoded in both C. ulcerans genomes. The rbp gene coding for a putative ribosome-binding protein with striking structural similarity to Shiga-like toxins was additionally detected in the genome of the human isolate C. ulcerans 809. The molecular data deduced from the complete genome sequences provides considerable knowledge of virulence factors in C. ulcerans that is increasingly recognized as an emerging pathogen. This bacterium is apparently equipped with a broad and varying set of virulence factors, including a novel type of a ribosome-binding protein. Whether the respective protein contributes to the severity of human infections (and a fatal outcome) remains to be elucidated by genetic experiments with defined bacterial mutants and host model systems