Optimal Survey Strategies and Predicted Planet Yields for the Korean Microlensing Telescope Network

The Korean Microlensing Telescope Network (KMTNet) will consist of three 1.6m telescopes each with a 4 deg^{2} field of view (FoV) and will be dedicated to monitoring the Galactic Bulge to detect exoplanets via gravitational microlensing. KMTNet's combination of aperture size, FoV, cadence, and longitudinal coverage will provide a unique opportunity to probe exoplanet demographics in an unbiased way. Here we present simulations that optimize the observing strategy for, and predict the planetary yields of, KMTNet. We find preferences for four target fields located in the central Bulge and an exposure time of t_{exp} = 120s, leading to the detection of ~2,200 microlensing events per year. We estimate the planet detection rates for planets with mass and separation across the ranges 0.1 <= M_{p}/M_{Earth} <= 1000 and 0.4 <= a/AU <= 16, respectively. Normalizing these rates to the cool-planet mass function of Cassan (2012), we predict KMTNet will be approximately uniformly sensitive to planets with mass 5 <= M_{p}/M_{Earth} <= 1000 and will detect ~20 planets per year per dex in mass across that range. For lower-mass planets with mass 0.1 <= M_{p}/M_{Earth} < 5, we predict KMTNet will detect ~10 planets per year. We also compute the yields KMTNet will obtain for free-floating planets (FFPs) and predict KMTNet will detect ~1 Earth-mass FFP per year, assuming an underlying population of one such planet per star in the Galaxy. Lastly, we investigate the dependence of these detection rates on the number of observatories, the photometric precision limit, and optimistic assumptions regarding seeing, throughput, and flux measurement uncertainties.Comment: 29 pages, 31 figures, submitted to ApJ. For a brief video explaining the key results of this paper, please visit: https://www.youtube.com/watch?v=e5rWVjiO26

TEDI: the TripleSpec Exoplanet Discovery Instrument

The TEDI (TripleSpec - Exoplanet Discovery Instrument) will be the first instrument fielded specifically for finding low-mass stellar companions. The instrument is a near infra-red interferometric spectrometer used as a radial velocimeter. TEDI joins Externally Dispersed Interferometery (EDI) with an efficient, medium-resolution, near IR (0.9 - 2.4 micron) echelle spectrometer, TripleSpec, at the Palomar 200" telescope. We describe the instrument and its radial velocimetry demonstration program to observe cool stars.Comment: 6 Pages, To Appear in SPIE Volume 6693, Techniques and Instrumentation for Detection of Exoplanets II

Distinct Pathways Mediate the Sorting of Tail-Anchored Proteins to the Plastid Outer Envelope

Background: Tail-anchored (TA) proteins are a distinct class of membrane proteins that are sorted post-translationally to various organelles and function in a number of important cellular processes, including redox reactions, vesicular trafficking and protein translocation. While the molecular targeting signals and pathways responsible for sorting TA proteins to their correct intracellular destinations in yeasts and mammals have begun to be characterized, relatively little is known about TA protein biogenesis in plant cells, especially for those sorted to the plastid outer envelope. Methodology/Principal Findings: Here we investigated the biogenesis of three plastid TA proteins, including the 33-kDa and 34-kDa GTPases of the translocon at the outer envelope of chloroplasts (Toc33 and Toc34) and a novel 9-kDa protein of unknown function that we define here as an outer envelope TA protein (OEP9). Using a combination of in vivo and in vitro assays we show that OEP9 utilizes a different sorting pathway than that used by Toc33 and Toc34. For instance, while all three TA proteins interact with the cytosolic OEP chaperone/receptor, AKR2A, the plastid targeting information within OEP9 is distinct from that within Toc33 and Toc34. Toc33 and Toc34 also appear to differ from OEP9 in that their insertion is dependent on themselves and the unique lipid composition of the plastid outer envelope. By contrast, the insertion of OEP9 into the plastid outer envelope occurs in a proteinaceous-dependent, but Toc33/34-independent manner and membrane lipids appear to serve primarily to facilitate normal thermodynamic integration of this TA protein. Conclusions/Significance: Collectively, the results provide evidence in support of at least two sorting pathways for plastid TA outer envelope proteins and shed light on not only the complex diversity of pathways involved in the targeting and insertion of proteins into plastids, but also the molecular mechanisms that underlie the delivery of TA proteins to their proper intracellular locations in general

Escherichia coli biofilms have an organized and complex extracellular matrix structure

Bacterial biofilms are ubiquitous in nature, and their resilience is derived in part from a complex extracellular matrix that can be tailored to meet environmental demands. Although common developmental stages leading to biofilm formation have been described, how the extracellular components are organized to allow three-dimensional biofilm development is not well understood. Here we show that uropathogenic Escherichia coli (UPEC) strains produce a biofilm with a highly ordered and complex extracellular matrix (ECM). We used electron microscopy (EM) techniques to image floating biofilms (pellicles) formed by UPEC. EM revealed intricately constructed substructures within the ECM that encase individual, spatially segregated bacteria with a distinctive morphology. Mutational and biochemical analyses of these biofilms confirmed curli as a major matrix component and revealed important roles for cellulose, flagella, and type 1 pili in pellicle integrity and ECM infrastructure. Collectively, the findings of this study elucidated that UPEC pellicles have a highly organized ultrastructure that varies spatially across the multicellular community

Evaluation and Treatment in Urology for Nocturia Caused by Nonurological Mechanisms:Guidance from the PLANET Study

Patients with nocturia are commonly referred to urology clinics, including many for whom a nonurological medical condition is responsible for their symptoms. The PLanning Appropriate Nocturia Evaluation and Treatment (PLANET) study was established to develop practical approaches to equip healthcare practitioners to deal with the diverse causes of nocturia, based on systematic reviews and expert consensus. Initial assessment and therapy need to consider the possibility of one or more medical conditions falling into the “SCREeN” areas of Sleep medicine (insomnia, periodic limb movements of sleep, parasomnias, and obstructive sleep apnoea), Cardiovascular (hypertension and congestive heart failure), Renal (chronic kidney disease), Endocrine (diabetes mellitus, thyroid disease, pregnancy/menopause, and diabetes insipidus), and Neurology. Medical and medication causes of xerostomia should also be considered. Some key indicators for these conditions can be identified in urology clinics, working in partnership with the primary care provider. Therapy of the medical condition in some circumstances lessens the severity of nocturia. However, in many cases there is a conflict between the two, in which case the medical condition generally takes priority on safety grounds. It is important to provide patients with a realistic expectation of therapy and awareness of limitations of current therapeutic options for nocturia.Patient summaryNocturia is the symptom of waking at night to pass urine. Commonly, this problem is referred to urology clinics. However, in some cases, the patient does not have a urological condition but actually a condition from a different speciality of medicine. This article describes how best the urologist and the primary care doctor can work together to assess the situation and make sensible and safe treatment suggestions. Unfortunately, there is sometimes no safe or effective treatment choice for nocturia, and treatment needs to focus instead on supportive management of symptoms

Optical modeling and polarization calibration for CMB measurements with ACTPol and Advanced ACTPol

The Atacama Cosmology Telescope Polarimeter (ACTPol) is a polarization sensitive upgrade to the Atacama Cosmology Telescope. Located at an elevation of 5190 m, ACTPol measures the Cosmic Microwave Background (CMB) temperature and polarization with arcminute-scale angular resolution. Calibration of the detector angles is a critical step in producing maps of the CMB polarization. Polarization angle offsets in the detector calibration can cause leakage in polarization from E to B modes and induce a spurious signal in the EB and TB cross correlations, which eliminates our ability to measure potential cosmological sources of EB and TB signals, such as cosmic birefringence. We present our optical modeling and measurements associated with calibrating the detector angles in ACTPol.Comment: 12 pages, 8 figures, conference proceedings submitted to Proceedings of SPIE; added reference in section 2 and merged repeated referenc

Biofouling and its control for in situ lab-on-a-chip marine environmental sensors

Biofouling is the process by which biological organisms attach to surfaces in an aqueous environment. This occurs on nearly all surfaces in all natural aquatic environments, and can cause problems with the functioning of scientific equipment exposed to the marine environment for extended periods. At the National Oceanographic Centre in Southampton (NOCS), the Centre for Marine Microsystems (CMM) is developing lab-on-chip micro-sensors to monitor the chemical and biological environment in situ in the oceans. Due to the long periods (up to several months) that these sensors will be deployed, biofouling by microbial biofilms is an important concern for the efficient running of these sensors. The aim of this project was therefore to determine the potential level of fouling within the sensors and to investigate the potential use of low-concentration diffusible molecules (LCDMs) to remediate biofouling.Many of the sensors in development by CMM are designed to sense specific chemical species and they use various chemical reagents to achieve this. The effects of some of these reagents on the formation of biofilms by mixed marine communities were investigated. It was shown that Griess reagent and ortho-phthadialdehyde (OPA), used to sense nitrites and ammonium respectively, effectively stop biofilm formation by killing microorganisms before they can attach to surfaces.Biofouling on two different polymers, cyclic olefin copolymer (COC) and poly (methyl methacrylate) (PMMA), used in the construction of micro-sensors, was compared with biofouling on glass. No differences were observed between COC and PMMA, however a small but significant difference in surface coverage was observed between glass and COC at the early stages of exposure to the marine environment. The lack of differences between the two polymers suggests that biofouling is not an important consideration when deciding whether to construct sensors from COC or PMMA. However, the larger degree of fouling on hydrophobic COC compared with hydrophilic glass indicates a potential use of surface modifications as an antifouling strategy.The effects on biofouling of the LCDMs nitric oxide (NO), cis-2-decenoic acid (CDA) and patulin, were investigated to evaluate their potential for anti-fouling in marine micro sensors. All three molecules were shown to reduce the formation of biofilms by mixed marine communities, but colony counts suggested that the effect of patulin was due to toxicity as opposed to a physiological effect. Investigation of biofilm growth in the light and the dark revealed that there was less biofilm formation in the light that the dark and this effect was determined to be due to an interaction with the polystyrene growth substratum.Analysis of the biofilm communities grown in the presence of LCDMs by denaturing gradient gel electrophoresis (DGGE), showed no clear differences in community profiles depending on the LCDMs. However those biofilms grown in the light appeared to have a greater proportion of Alphaproteobacteria than those grown in the dark.Further study is needed to determine the level of fouling and the applicability of LCDMs in real micro-sensor systems. However, this study has shown that LCDMs have the potential to remediate, at least in part, the biofouling of marine micro-sensors