A Wide-Field View of Leo II -- A Structural Analysis Using the SDSS

Using SDSS I data, we have analysed the stellar distribution of the Leo II dwarf spheroidal galaxy (distance of 233 kpc) to search for evidence of tidal deformation. The existing SDSS photometric catalogue contains gaps in regions of high stellar crowding, hence we filled the area at the centre of Leo II using the DAOPHOT algorithm applied to the SDSS images. The combined DAOPHOT-SDSS dataset contains three-filter photometry over a 4x4 square degree region centred on Leo II. By defining a mask in three-filter colour-magnitude space, we removed the majority of foreground field stars. We have measured the following Leo II structural parameters: a core radius of r_c = 2.64 +/- 0.19 arcmin (178 +/- 13 pc), a tidal radius of r_t = 9.33 +/- 0.47 arcmin (632 +/- 32 pc) and a total V-band luminosity of L_V = (7.4 +/- 2.0) times 10^5 L_sun (M_V = -9.9 +/- 0.3). Our comprehensive analysis of the Leo II structure did not reveal any significant signs of tidal distortion. The internal structure of this object contains only mild isophotal twisting. A small overdensity was discovered appoximately 4.5 tidal radii from the Leo II centre, however we conclude it is unlikely to be material tidally stripped from Leo II based on its stellar population, and is most likely a foreground overdensity of stars. Our results indicate that the influence of the Galactic graviational field on the structure of Leo II has been relatively mild. We rederived the mass-to-light ratio of this system using existing kinematic data combined with our improved structural measurements, and favour the scenario in which Leo II is strongly dominated by dark matter with (M/L)_V ~ 100 in solar units.Comment: 41 pages, 15 figures, accepted for publication in the Astronomical Journa

The Nature of the Density Clump in the Fornax Dwarf Spheroidal Galaxy

We have imaged the recently discovered stellar overdensity located approximately one core radius from the center of the Fornax dwarf spheroidal galaxy using the Magellan Clay 6.5m telescope with the Magellan Instant Camera (MagIC). Superb seeing conditions allowed us to probe the stellar populations of this overdensity and of a control field within Fornax to a limiting magnitude of R=26. The color-magnitude diagram of the overdensity field is virtually identical to that of the control field with the exception of the presence of a population arising from a very short (less than 300 Myr in duration) burst of star formation 1.4 Gyr ago. Coleman et al. have argued that this overdensity might be related to a shell structure in Fornax that was created when Fornax captured a smaller galaxy. Our results are consistent with this model, but we argue that the metallicity of this young component favors a scenario in which the gas was part of Fornax itself.Comment: 24 pages including 8 figures and 3 tables. Accepted by Astronomical Journa

Large-scale Spatiotemporal Spike Patterning Consistent with Wave Propagation in Motor Cortex

Aggregate signals in cortex are known to be spatiotemporally organized as propagating waves across the cortical surface, but it remains unclear whether the same is true for spiking activity in individual neurons. Furthermore, the functional interactions between cortical neurons are well documented but their spatial arrangement on the cortical surface has been largely ignored. Here we use a functional network analysis to demonstrate that a subset of motor cortical neurons in non-human primates spatially coordinate their spiking activity in a manner that closely matches wave propagation measured in the beta oscillatory band of the local field potential. We also demonstrate that sequential spiking of pairs of neuron contains task-relevant information that peaks when the neurons are spatially oriented along the wave axis. We hypothesize that the spatial anisotropy of spike patterning may reflect the underlying organization of motor cortex and may be a general property shared by other cortical areas

Constructive activism in the dark web: cryptomarkets and illicit drugs in the digital ‘demimonde’

This paper explores activism enacted through Silk Road, a now defunct cryptomarket where illicit drugs were sold in the dark web. Drawing on a digital ethnography of Silk Road, we develop the notion of constructive activism to extend the lexicon of concepts available to discuss forms of online activism. Monitoring of the cryptomarket took place between June 2011 and its closure in October 2013. Just before and after the closure of the marketplace we conducted anonymous online interviews with 17 people who reported buying drugs on Silk Road (1.0). These interviews were conducted synchronously and interactively through encrypted instant messaging. Participants discussed harnessing and developing the technological tools needed to access Silk Road and engage within the Silk Road community. For participants Silk Road was not just a market for trading drugs: it facilitated a shared experience of personal freedom within a libertarian philosophical framework, where open discussions about stigmatized behaviours were encouraged and supported. Tensions between public activism against drug prohibition and the need to hide one's identity as a drug user from public scrutiny were partially resolved through community actions that internalized these politics, rather than engaging in forms of online activism that are intended to have real-world political effects. Most aptly described through van de Sande's (2015) concept of prefigurative politics, they sought to transform their values into built environments that were designed to socially engineer a more permissive digital reality, which we refer to as constructive activism

A Deep Survey of the Fornax dSph I: Star Formation History

Based on a deep imaging survey, we present the first homogeneous star formation history (SFH) of the Fornax dwarf spheroidal (dSph) galaxy. We have obtained two-filter photometry to a depth of B ~ 23 over the entire surface of Fornax, the brightest dSph associated with the Milky Way, and derived its SFH using a CMD-fitting technique. We show that Fornax has produced the most complex star formation and chemical enrichment histories of all the Milky Way dSphs. This system has supported multiple epochs of star formation. A significant number of stars were formed in the early Universe, however the most dominant population are the intermediate age stars. This includes a strong burst of star formation approximately 3 to 4 Gyr ago. Significant population gradients are also evident. Similar to other dSphs, we have found that recent star formation was concentrated towards the centre of the system. Furthermore, we show that the central region harboured a faster rate of chemical enrichment than the outer parts of Fornax. At the centre, the ancient stars (age > 10 Gyr) display a mean metallicity of [Fe/H] ~ -1.4, with evidence for three peaks in the metallicity distribution. Overall, enrichment in Fornax has been highly efficient: the most recent star formation burst has produced stars with close to solar metallicity. Our results support a scenario in which Fornax experienced an early phase of rapid chemical enrichment producing a wide range of abundances. Star formation gradually decreased until ~4 Gyr ago, when Fornax experienced a sudden burst of strong star formation activity accompanied by substantial chemical enrichment. Weaker star forming events followed, and we have found tentative evidence for stars with ages less than 100 Myr.Comment: 13 pages, 13 figures, accepted for publication in Ap

SOST Inhibits Prostate Cancer Invasion.

Inhibitors of Wnt signaling have been shown to be involved in prostate cancer (PC) metastasis; however the role of Sclerostin (Sost) has not yet been explored. Here we show that elevated Wnt signaling derived from Sost deficient osteoblasts promotes PC invasion, while rhSOST has an inhibitory effect. In contrast, rhDKK1 promotes PC elongation and filopodia formation, morphological changes characteristic of an invasive phenotype. Furthermore, rhDKK1 was found to activate canonical Wnt signaling in PC3 cells, suggesting that SOST and DKK1 have opposing roles on Wnt signaling in this context. Gene expression analysis of PC3 cells co-cultured with OBs exhibiting varying amounts of Wnt signaling identified CRIM1 as one of the transcripts upregulated under highly invasive conditions. We found CRIM1 overexpression to also promote cell-invasion. These findings suggest that bone-derived Wnt signaling may enhance PC tropism by promoting CRIM1 expression and facilitating cancer cell invasion and adhesion to bone. We concluded that SOST and DKK1 have opposing effects on PC3 cell invasion and that bone-derived Wnt signaling positively contributes to the invasive phenotypes of PC3 cells by activating CRIM1 expression and facilitating PC-OB physical interaction. As such, we investigated the effects of high concentrations of SOST in vivo. We found that PC3-cells overexpressing SOST injected via the tail vein in NSG mice did not readily metastasize, and those injected intrafemorally had significantly reduced osteolysis, suggesting that targeting the molecular bone environment may influence bone metastatic prognosis in clinical settings

Circulating microRNAs as biomarkers to assist the management of the malignant germ-cell-tumour subtype choriocarcinoma.

Germ-cell-tumours (GCTs) are heterogeneous and management is complex. The current conventional biomarkers, alpha-fetoprotein and human-chorionic-gonadotropin (HCG), have limited utility for diagnosis/follow-up as secretion is restricted to specific malignant-GCT subtypes and long half-life can make interpretation and clinical decision-making challenging. We sought to identify circulating microRNAs that reflected choriocarcinoma disease activity more accurately than HCG in a metastatic primary mediastinal nonseminomatous-GCT (PMNSGCT) case with elevated diagnostic serum HCG (>250,000U/L), consistent with pure choriocarcinoma. We undertook comprehensive microRNA profiling (n=754 microRNAs) using two 384-well TaqMan Low-Density-Array cards in 16 serum samples; 10 from PMNSGCT diagnosis/follow-up and six controls. Key findings underwent confirmatory qRT-PCR. We identified a serum panel of choriocarcinoma-specific ‘chromosome-19-microRNA-cluster’ (C19MC) microRNAs that were highly elevated at diagnosis but fell rapidly on treatment and normalised before the second full chemotherapy course. We also re-confirmed serum elevation of the previously identified malignant-GCT marker miR-371a-3p at diagnosis. These circulating microRNA markers reflected choriocarcinoma disease activity more accurately than serum HCG and real-time knowledge would have assisted clinical decision-making. With further study, these microRNA markers will facilitate future management of such patients and are likely to result in improved outcomes.Acknowledgment of research support: The authors acknowledge grant funding from the St. Baldrick’s Foundation [reference 358099], the Isaac Newton Trust [reference 15.40f], the Medical Research Council [reference MR/R001146/1] and Addenbrooke’s Charitable Trust [reference 23/17 B (iv)]. We are grateful for support from the Max Williamson Fund and from Christiane and Alan Hodson, in memory of their daughter Olivia. The funders were not involved in study design, data collection or interpretation, or decision to submit for publication