Bias in the prediction of genetic gain due to mass and half-sib selection in random mating populations

The prediction of gains from selection allows the comparison of breeding methods and selection strategies, although these estimates may be biased. The objective of this study was to investigate the extent of such bias in predicting genetic gain. For this, we simulated 10 cycles of a hypothetical breeding program that involved seven traits, three population classes, three experimental conditions and two breeding methods (mass and half-sib selection). Each combination of trait, population, heritability, method and cycle was repeated 10 times. The predicted gains were biased, even when the genetic parameters were estimated without error. Gain from selection in both genders is twice the gain from selection in a single gender only in the absence of dominance. The use of genotypic variance or broad sense heritability in the predictions represented an additional source of bias. Predictions based on additive variance and narrow sense heritability were equivalent, as were predictions based on genotypic variance and broad sense heritability. The predictions based on mass and family selection were suitable for comparing selection strategies, whereas those based on selection within progenies showed the largest bias and lower association with the realized gain

NeuMa - the absolute Neuromarketing dataset en route to an holistic understanding of consumer behaviour

Neuromarketing is a continuously evolving field that utilises neuroimaging technologies to explore consumers’ behavioural responses to specific marketing-related stimulation, and furthermore introduces novel marketing tools that could complement the traditional ones like questionnaires. In this context, the present paper introduces a multimodal Neuromarketing dataset that encompasses the data from 42 individuals who participated in an advertising brochure-browsing scenario. In more detail, participants were exposed to a series of supermarket brochures (containing various products) and instructed to select the products they intended to buy. The data collected for each individual executing this protocol included: (i) encephalographic (EEG) recordings, (ii) eye tracking (ET) recordings, (iii) questionnaire responses (demographic, profiling and product related questions), and (iv) computer mouse data. NeuMa dataset has both dynamic and multimodal nature and, due to the narrow availability of open relevant datasets, provides new and unique opportunities for researchers in the field to attempt a more holistic approach to neuromarketing

Escherichia coli genome-wide promoter analysis: Identification of additional AtoC binding target elements

<p>Abstract</p> <p>Background</p> <p>Studies on bacterial signal transduction systems have revealed complex networks of functional interactions, where the response regulators play a pivotal role. The AtoSC system of <it>E. coli </it>activates the expression of <it>atoDAEB </it>operon genes, and the subsequent catabolism of short-chain fatty acids, upon acetoacetate induction. Transcriptome and phenotypic analyses suggested that <it>atoSC </it>is also involved in several other cellular activities, although we have recently reported a palindromic repeat within the <it>atoDAEB </it>promoter as the single, <it>cis</it>-regulatory binding site of the AtoC response regulator. In this work, we used a computational approach to explore the presence of yet unidentified AtoC binding sites within other parts of the <it>E. coli </it>genome.</p> <p>Results</p> <p>Through the implementation of a computational <it>de novo </it>motif detection workflow, a set of candidate motifs was generated, representing putative AtoC binding targets within the <it>E. coli </it>genome. In order to assess the biological relevance of the motifs and to select for experimental validation of those sequences related robustly with distinct cellular functions, we implemented a novel approach that applies Gene Ontology Term Analysis to the motif hits and selected those that were qualified through this procedure. The computational results were validated using Chromatin Immunoprecipitation assays to assess the <it>in vivo </it>binding of AtoC to the predicted sites. This process verified twenty-two additional AtoC binding sites, located not only within intergenic regions, but also within gene-encoding sequences.</p> <p>Conclusions</p> <p>This study, by tracing a number of putative AtoC binding sites, has indicated an AtoC-related cross-regulatory function. This highlights the significance of computational genome-wide approaches in elucidating complex patterns of bacterial cell regulation.</p

Efeito da reeducação postural global no alinhamento corporal e nas condições clínicas de indivíduos com disfunção temporomandibular associada a desvios posturais Effect of global postural reeducation on body alignment and on clinical status of individuals with temporomandibular disorder associated to postural deviations

Este estudo visou verificar o efeito da técnica de reeducação postural global (RPG) nas condições físicas, psicológicas e psicossociais, assim como no alinhamento corporal, de indivíduos com disfunção temporomandibular (DTM) associada a desvio postural. Participaram 20 indivíduos com DTM e com desvio postural confirmado por exame físico, avaliados, antes e depois do tratamento de RPG, pelos critérios diagnósticos de desordens temporomandibulares (RDC/TMD, na sigla em inglês) e quanto às medidas angulares, por fotogrametria digital. O tratamento consistiu em 10 sessões semanais de RPG. Os resultados após o tratamento mostram, na classificação da disfunção, maior predomínio de desordens apenas musculares (em detrimento das articulares e por deslocamento de disco) e redução da intensidade da dor orofacial; o percentual de indivíduos sem depressão aumentou de 10% para 35%; o percentual de indivíduos com classificação normal de sintomas físicos (excluindo itens de dor) passou de 30% para 55%. Foi encontrada melhora estatisticamente significante na maioria das medidas angulares, exceto nos ângulos frontais dos membros inferiores e ângulo perna/retropé direito. O alinhamento horizontal da cabeça e as medidas de lordose cervical e lombar, com valores normais antes da RPG, não se modificaram. Conclui-se que, com o tratamento de RPG, os indivíduos apresentaram importantes melhoras dos sintomas físicos e psicológicos da DTM, assim como melhora do alinhamento e simetria corporais.<br>The purpose of this study was to assess the effects of the technique of global postural re-education (GPR) on body alignment and clinical status of individuals with temporomandibular disorder (TMD) associated to postural deviations. Twenty individuals with both TMD and postural deviations confirmed by physical examination were assessed, before and after treatment, by the research diagnostic criteria for temporomandibular disorders (RDC/TMD) and as to body angle measures, by digital photogrammetry. Treatment consisted of 10 weekly, 45-minute GPR sessions. Results after treatment show higher predominance of myogenic dysfunction (rather than joint or disc ones) and significant orofacial pain intensity relief; the percentage of individuals without depression raised from10% to 35%; the percentage of individuals with physical symptoms classified as normal (excluding pain items) raised from 30 to 55%. A statistically significant improvement was found in most body structures angle measures, except at lower limb frontal angles and at the right leg/foot angle. Head alignment and measures of cervical and lumbar lordosis, having normal values before treatment, did not change. Hence the GPR treatment may be said to have brought significant relief of TMD physical and psychological symptoms, as well as improvement in body alignment and symmetry