Improve the Capacities of Zimbabwe for the Control of animal and zoonotic diseases

The main objective of the CAZCOM project is to build Zimbabwe's capacity to improve the surveillance and control of important animal and zoonotic diseases. Environmental changes related to climate change have a major impact on the epidemiology and emergence of vector-borne and non-vector-borne diseases in humans, domestic animals and wildlife. The human, societal, environmental and economic costs associated with changes in the dynamics of infectious diseases are considerable. Zimbabwe, a country located in the subtropical area, will have to face many human and veterinary sanitary challenges in connection with these global changes. Currently, the lack of resources, skilled personnel, and advanced biotechnology infrastructures does not allow the country to put in place an adequate disease surveillance and health response. The CAZCOM project, through its training activities, the establishment of a laboratory with international standards, the development of research projects and the setting-up of effective surveillance and control systems for infectious diseases aims to increase Zimbabwe's autonomy for the control of animal diseases, within the frame of its national livestock breeding strategy. CAZCOM will (1) develop molecular biology technical capacities by creating a molecular platform, training technical staff and establishing technical private/public partnerships, (2) provide training through the development of technical trainings and master modules, the revision of master curricula and the supervision of master students, (3) enhance efficiency and autonomy of animal and zoonotic disease surveillance systems through the set-up of sampling protocols to characterize disease circulation and inter-species transmission modes a at the Human - Wildlife – Livestock interfaces, the development of diagnostic tools and the identification of new surveillance strategies. The success of this project is based on existing collaborations between CIRAD, IRD and their local and international partners developed over the past years through the "Partnership Production and Conservation Research Platform" (RP-PCP). The RP-PCP has established links with Zimbabwe's leading universities as well as with key departments of the Ministry of Agriculture and the Environment. This network enables the RP-PCP and all its partners to create joint research projects and benefit from a regional research dynamic

Brucellosis and chlamydiosis seroprevalence in goats at livestock–wildlife interface areas of Zimbabwe

In Zimbabwe, there have been no chlamydiosis and limited brucellosis studies in goats. This study was conducted to determine the seroprevalence and risk factors of the two diseases in goats at three different livestock–wildlife interface areas: porous, non-porous and non-interface in the south-eastern lowveld of Zimbabwe. Collected sera (n = 563) were tested for Brucella antibodies using the Rose Bengal plate test (RBPT) and the complement fixation test (CFT); and for Chlamydia abortus antibodies using the CFT. All tested goats were negative for Brucella antibodies. Overall, chlamydial seroprevalence was 22%. The porous [c2 = 9.6, odds ratio (OR) = 2.6, p = 0.002] and non-porous (c2 = 37.5, OR = 5.8, p < 0.00001) interfaces were approximately three and six times more likely to be chlamydial seropositive than the non-interface area, respectively. Chlamydial seroprevalence was not associated with sex (c2 = 0.5, OR = 1.2, p = 0.5), abortion history in female goats (c2 = 0.7, OR = 1.3, p = 0.4), keeping goats with cattle (c2 = 0.2, OR = 1.5, p = 0.7) or flock size (c2 = 0.03, OR = 1.4, p = 0.9). Our study provides the first serological evidence of chlamydiosis in goats in Zimbabwe and the results suggest that proximity to wildlife is associated with increased chlamydial seropositivity. Further studies are required to determine the role of chlamydial infection on goat reproductive failure and that of wildlife on C. abortus transmission to domestic ruminants

Serological survey of <i>Brucella canis</i> in dogs in urban Harare and selected rural communities in Zimbabwe

A cross-sectional study was conducted in order to detect antibodies for Brucella canis (B. canis) in dogs from urban Harare and five selected rural communities in Zimbabwe. Sera from randomly selected dogs were tested for antibodies to B. canis using an enzyme-linked immunosorbent assay. Overall, 17.6% of sera samples tested (57/324, 95% CI: 13.5–21.7) were positive for B. canis antibodies. For rural dogs, seroprevalence varied from 11.7% – 37.9%. Rural dogs recorded a higher seroprevalence (20.7%, 95% CI: 15.0–26.4) compared with Harare urban dogs (12.7%, 95% CI: 6.9–18.5) but the difference was not significant (p = 0.07). Female dogs from both sectors had a higher seroprevalence compared with males, but the differences were not significant (p > 0.05). Five and two of the positive rural dogs had titres of 1:800 and 1:1600, respectively, whilst none of the positive urban dogs had a titre above 1:400. This study showed that brucellosis was present and could be considered a risk to dogs from the studied areas. Further studies are recommended in order to give insight into the epidemiology of brucellosis in dogs and its possible zoonotic consequences in Zimbabwe. Screening for other Brucella spp. (Brucella abortus, Brucella melitensis and Brucella suis) other than B. canis is also recommended

Micro-milling work-holding devices employing adhesive forces

IngenieursweseBedryfsingenieursweseMicro-parts are often very fragile rendering conventional, mechanical work-holding fixtures unsuitable for them since they exert large straining forces. Furthermore, macroworkholding devices occupy a large space which impedes high precision required in micromilling. Although some micro-clamping fixtures exist, they are limited to specific part shapes and in most cases expensive to manufacture. Hence this paper focuses on the application of adhesive forces namely electrostatic, surface tension and van-der-Waals forces; in work-holding strategies for micro-milling operations. An analysis is given as to their applicability with reference to micro-milling cutting forces

Assessment of community awareness and risk perceptions of zoonotic causes of abortion in cattle at three selected livestock–wildlife interface areas of Zimbabwe

A study was conducted to assess the awareness of cattle abortions due to brucellosis, Rift Valley fever (RVF) and leptospirosis, and to compare frequencies of reported abortions in communities living at the periphery of the Great Limpopo Transfrontier Conservation Area in southeastern Zimbabwe. Three study sites were selected based on the type of livestock–wildlife interface: porous livestock–wildlife interface (unrestricted); non-porous livestock–wildlife interface (restricted by fencing); and livestock–wildlife non-interface (totally absent or control). Respondents randomly selected from a list of potential cattle farmers (N = 379) distributed at porous (40·1%), non-interface (35·5%) and non-porous (26·4%), were interviewed using a combined close- and open-ended questionnaire. Focus group discussions were conducted with 10–12 members of each community. More abortions in the last 5 years were reported from the porous interface (52%) and a significantly higher per cent of respondents from the porous interface (P < 0·05) perceived wildlife as playing a role in livestock abortions compared with the other interface types. The odds of reporting abortions in cattle were higher in large herd sizes (odds ratio (OR) = 2·6; 95% confidence interval (CI) 1·5–4·3), porous (OR = 1·9; 95% CI 1·0–3·5) and non-porous interface (OR = 2·2; 95% CI 1·1–4·3) compared with livestock–wildlife non-interface areas. About 21·6% of the respondents knew brucellosis as a cause of abortion, compared with RVF (9·8%) and leptospirosis (3·7%). These results explain to some extent, the existence of human/wildlife conflict in the studied livestock–wildlife interface areas of Zimbabwe, which militates against biodiversity conservation efforts. The low awareness of zoonoses means the public is at risk of contracting some of these infections. Thus, further studies should focus on livestock–wildlife interface areas to assess if the increased rates of abortions reported in cattle may be due to exposure to wildlife or other factors. The government of Zimbabwe needs to launch educational programmes on public health awareness in these remote areas at the periphery of transfrontier conservation areas where livestock–wildlife interface exists to help mitigate the morbidity and mortality. (Résumé d'auteur

Transfer of Individual Micro- and Nanoparticles for High- Precision 3D Analysis Using 360° Electron Tomography

A versatile approach is demonstrated, providing a general routine for an extensive and advanced 3D characterization of individually selected micro- and nanoparticles, enabling the combination of complementary and scale-bridging techniques. Quintessential to the method is the transfer of individual particles onto tailored tips using a conventional scanning electron microscope equipped with a suitable micromanipulator. The method enables a damage- and contamination-free preparation of freestanding particles. This is of significant importance for applications addressing the measurement of structural, physical, and chemical properties of specifically selected particles, such as 360° electron tomography, atom probe tomography, nano X-ray tomography, or optical near-field measurements. In this context, the method is demonstrated for 360° electron tomography of micro-/macroporous zeolite particles with sizes in the micrometer range and mesoporous alpha-hematite nanoparticles exhibiting sizes of 50–100 nm, including detailed pre- and postcharacterization on the nanoscale.“Deutsche Forschungsgemeinschaft” (DFG) within the framework of the SPP 1570 (project DFG SP 648/4-3 “3D analysis of complex pore structures using ET and high-resolution TEM”) and the research training group GRK 1896 (“In situ Microscopy with Electrons, X-rays and Scanning Probes”) as well as through the Cluster of Excellence “Engineering of Advanced Materials” at the Friedrich-Alexander-Universität Erlangen-Nürnberg (Germany)FIBJulian Losche

Youth perspectives of achievement: Is money everything?

This article draws from a qualitative research project completed at Victoria High School (pseudonym) in Cape Town in 2012 which explored 13 learners’ perspectives of achievement and its influence on their lives and thinking. The piece problematises and analyses taken-for-granted connections between money, achievement, youth aspirations and views of employment (Opsahl &amp; Dunnette, 1966). The article builds on McClelland’s (1967: 10) view that “money isn’t everything,” that money is a motivator for some, yet often inconsequential for others, and that its meaning mostly lies “in the eye of the beholder”. In light of this view, the articles discusses the perspectives of four learners at Victoria High to illustrate how they approached achievement, aspiration and materiality according to the different social standings and worlds that they inhabited. It was found that the expressed views of achievement by learners went beyond stereotypical and measurable attitudes and connected in quite complex ways with how they imagined their futures. The learners approached the notion of achievement in developmental, cumulative, and progressive ways. An interpretive qualitative paradigm using the work of Pierre Bourdieu and Arjun Appadurai was employed to highlight how the youth’s various capitals and aspirations respectively influenced their notions of achievement

Women teachers leading change against intimate partner violence at a state university in Zimbabwe

This study responded to the following research question: How can participatory and visual methodology enable women teachers to lead change against intimate partner violence at a state university in Zimbabwe? The study was qualitative and informed by the critical paradigm and employed visual participatory research methodology (PVM). The participants in this study were six women teachers from a state university who were selected by means of snowballing. The visual methods of data generation that were employed were memory accounts, drawings, participatory videos and focus group discussions. Memory accounts expressed the participants’ experiences and understanding of IPV as they made meaning of their lived realities. Data generated from the drawings depicted the participants’ positioning and the way in which they were positioned in intimate relationships. The participatory video was used to express the participants’ understanding of IPV and the critical steps that can be taken to address the issue. The findings indicated that the participants understand IPV as a complex issue and they can engage in and explore issues that affect them. The participants’ voices can initiate change by breaking down barriers as they address the issue. The participants also indicated that students’ voices should be heard in the drafting of policies to include issues that affect them directly. The findings suggest that engagement with women, listening to one another, being listened to and working collaboratively to offer solutions to problems as agents of change, empowers the women. The findings have implications for the university community in that they show that the women teachers are actors who are aware of IPV and can participate and inform intervention programs. The participants can lead change through their work as educators as they continue to share with the community. This could enhance knowledge co-production. I conclude by arguing that using PVM provided a safe space for the women to talk about their lived realities of IPV with a wider audience and propose solutions to a phenomenon that has always been taboo; thus leading change towards an IPV free society

A bioassay method validation framework for laboratory and semi-field tests used to evaluate vector control tools

Vector control interventions play a fundamental role in the control and elimination of vector-borne diseases. The evaluation of vector control products relies on bioassays, laboratory and semi-field tests using live insects to assess the product’s effectiveness. Bioassay method development requires a rigorous validation process to ensure that relevant methods are used to capture appropriate entomological endpoints which accurately and precisely describe likely efficacy against disease vectors as well as product characteristics within the manufacturing tolerance ranges for insecticide content specified by the World Health Organization. Currently, there are no standardized guidelines for bioassay method validation in vector control. This report presents a framework for bioassay validation that draws on accepted validation processes from the chemical and healthcare fields and which can be applied for evaluating bioassays and semi-field tests in vector control. The validation process has been categorized into four stages: preliminary development; feasibility experiments; internal validation, and external validation. A properly validated method combined with an appropriate experimental design and data analyses that account for both the variability of the method and the product is needed to generate reliable estimates of product efficacy to ensure that at-risk communities have timely access to safe and reliable vector control products.</p

An assessment of factors that determine usage of contraceptive services among adolescent women in Lesotho: An analytical cross-sectional study of Maseru city secondary school students

Master of Public Health - MPHIn the Maseru district, the high prevalence of unintended pregnancies among adolescents aged between 15 to 19 years may be due to non-use of modern contraceptive methods. It is therefore important to investigate possible barriers adolescents in Maseru in Lesotho face in accessing contraceptive methods and services. This research has therefore focused on the factors that affect the accessibility and usage of contraceptive services among in-school adolescent girls in Maseru City, Lesotho.The study was an observational, analytical, cross-sectional study. A sample of 10 high schools in Maseru city was selected as study sites, using computer-generated random numbers. A stratified random sampling method was used to enrol a total of 368 sexually active respondents, aged 15-19 years into the study. Data was collected using a structured one-on-one interviewer administered questionnaire and analysis was conducted using the STATA 14 statistical software programme. Ethical approval was obtained from the Biomedical Research and Ethics Committee (University of the Western Cape) and the Lesotho Ministry of Health and Social Welfare. Written parental/caregiver informed consent and written participant consent and assent, as per the Lesotho law were obtained