Determination of Ligand Profiles for Pseudomonas aeruginosa Solute Binding Proteins

Solute binding proteins (SBPs) form a heterogeneous protein family that is found in all kingdoms of life. In bacteria, the ligand-loaded forms bind to transmembrane transporters providing the substrate. We present here the SBP repertoire of Pseudomonas aeruginosa PAO1 that is composed of 98 proteins. Bioinformatic predictions indicate that many of these proteins have a redundant ligand profile such as 27 SBPs for proteinogenic amino acids, 13 proteins for spermidine/putrescine, or 9 proteins for quaternary amines. To assess the precision of these bioinformatic predictions, we have purified 17 SBPs that were subsequently submitted to high-throughput ligand screening approaches followed by isothermal titration calorimetry studies, resulting in the identification of ligands for 15 of them. Experimentation revealed that PA0222 was specific for y-aminobutyrate (GABA), DppA2 for tripeptides, DppA3 for dipeptides, CysP for thiosulphate, OpuCC for betaine, and AotJ for arginine. Furthermore, RbsB bound D-ribose and D-allose, ModA bound molybdate, tungstate, and chromate, whereas AatJ recognized aspartate and glutamate. The majority of experimentally identified ligands were found to be chemoattractants. Data show that the ligand class recognized by SPBs can be predicted with confidence using bioinformatic methods, but experimental work is necessary to identify the precise ligand profile.This work was supported by FEDER funds and Fondo Social Europeo through a grant from the Spanish Ministry for Economy and Competitiveness to T. Krell (BIO2016-76779-P). This funding source was not involved in the design and conduct of this stud

Hacia la excelencia en la docencia de producción animal: empleo de técnicas de aprendizaje activo para la gestión integral de explotaciones ganaderas

El objetivo de este Proyecto de Innovación Docente es crear un equipo de trabajo orientado al desarrollo de metodologías de aprendizaje activo para la gestión de explotaciones ganaderas en la ETS de Ingenierías Agrarias de la Universidad de Valladolid (Campus de Palencia). Se pretende que los alumnos adquieran la competencia en gestión de explotaciones ganaderas desde un enfoque profesional. Se incluye una guía para el manejo del programa GID (MSD Animal Health), un modelo de informe técnico profesional y la rúbrica de evaluación del informeDepartamento de Ciencias Agroforestale

El podcast como herramienta de aprendizaje: Contribuciones de la Química Farmacéutica en el entorno “One Health”

Este proyecto se presenta como continuación del proyecto de innovación docente Nº 96 (2022/2023) y tiene como objetivo crear un modelo didáctico basado en el aprendizaje autónomo y práctico del alumnado mediante la escucha, elaboración y/o edición de podcasts, abordando temas relacionados con la importancia de la Química Farmacéutica en el campo de la salud y el bienestar.Depto. de Química en Ciencias FarmacéuticasFac. de FarmaciaFac. de Ciencias QuímicasFac. de Ciencias de la InformaciónFALSEsubmitte

El derecho público en Iberoamérica. Libro homenaje al profesor Jaime Vidal Perdomo. Tomos I y II

La obra que se entrega hoy es un gran ejemplo de la riqueza académica con la que todos cuentan en Iberoamérica, motor de una nueva integración, que mueve y motiva por encima de intereses políticos y partidarios. Para una mejor lectura de las evoluciones y las expectativas en derecho público en Iberoamérica, así como del balance del derecho administrativo colombiano, en sus primeros cien años de justicia contencioso-administrativa, en este orden de ideas se ha dividido la publicación en dos tomos. El primero de ellos se divide en tres partes: derecho constitucional, teoría general del derecho administrativo, y acto y procedimiento administrativo. El segundo tomo viene distribuido en cinco partes: la primera parte se ha denominado contratos administrativos, la segunda parte régimen local, la tercera parte, denominada bienes del Estado, la cuarta parte, contencioso-administrativo y la última parte, se ha descrito como temas varios. En ella se encuentran reflexiones sobre la evolución de la teoría del daño especial en Colombia. Con lo anterior, se espera haber cumplido con un buen balance bicentenario del derecho público en Iberoamérica y del derecho administrativo colombiano en estos cien primeros años de justicia contencioso-administrativa, en homenaje al maestro Jaime Vidal Perdomo, eximio jurista colombiano consagrado al estudio y análisis del derecho público y exponente calificado y reconocido de la academia en Colombia y en los países iberoamericanos

Global variation in postoperative mortality and complications after cancer surgery: a multicentre, prospective cohort study in 82 countries

© 2021 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY-NC-ND 4.0 licenseBackground: 80% of individuals with cancer will require a surgical procedure, yet little comparative data exist on early outcomes in low-income and middle-income countries (LMICs). We compared postoperative outcomes in breast, colorectal, and gastric cancer surgery in hospitals worldwide, focusing on the effect of disease stage and complications on postoperative mortality. Methods: This was a multicentre, international prospective cohort study of consecutive adult patients undergoing surgery for primary breast, colorectal, or gastric cancer requiring a skin incision done under general or neuraxial anaesthesia. The primary outcome was death or major complication within 30 days of surgery. Multilevel logistic regression determined relationships within three-level nested models of patients within hospitals and countries. Hospital-level infrastructure effects were explored with three-way mediation analyses. This study was registered with ClinicalTrials.gov, NCT03471494. Findings: Between April 1, 2018, and Jan 31, 2019, we enrolled 15 958 patients from 428 hospitals in 82 countries (high income 9106 patients, 31 countries; upper-middle income 2721 patients, 23 countries; or lower-middle income 4131 patients, 28 countries). Patients in LMICs presented with more advanced disease compared with patients in high-income countries. 30-day mortality was higher for gastric cancer in low-income or lower-middle-income countries (adjusted odds ratio 3·72, 95% CI 1·70–8·16) and for colorectal cancer in low-income or lower-middle-income countries (4·59, 2·39–8·80) and upper-middle-income countries (2·06, 1·11–3·83). No difference in 30-day mortality was seen in breast cancer. The proportion of patients who died after a major complication was greatest in low-income or lower-middle-income countries (6·15, 3·26–11·59) and upper-middle-income countries (3·89, 2·08–7·29). Postoperative death after complications was partly explained by patient factors (60%) and partly by hospital or country (40%). The absence of consistently available postoperative care facilities was associated with seven to 10 more deaths per 100 major complications in LMICs. Cancer stage alone explained little of the early variation in mortality or postoperative complications. Interpretation: Higher levels of mortality after cancer surgery in LMICs was not fully explained by later presentation of disease. The capacity to rescue patients from surgical complications is a tangible opportunity for meaningful intervention. Early death after cancer surgery might be reduced by policies focusing on strengthening perioperative care systems to detect and intervene in common complications. Funding: National Institute for Health Research Global Health Research Unit

Effects of hospital facilities on patient outcomes after cancer surgery: an international, prospective, observational study

© 2022 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 licenseBackground: Early death after cancer surgery is higher in low-income and middle-income countries (LMICs) compared with in high-income countries, yet the impact of facility characteristics on early postoperative outcomes is unknown. The aim of this study was to examine the association between hospital infrastructure, resource availability, and processes on early outcomes after cancer surgery worldwide. Methods: A multimethods analysis was performed as part of the GlobalSurg 3 study—a multicentre, international, prospective cohort study of patients who had surgery for breast, colorectal, or gastric cancer. The primary outcomes were 30-day mortality and 30-day major complication rates. Potentially beneficial hospital facilities were identified by variable selection to select those associated with 30-day mortality. Adjusted outcomes were determined using generalised estimating equations to account for patient characteristics and country-income group, with population stratification by hospital. Findings: Between April 1, 2018, and April 23, 2019, facility-level data were collected for 9685 patients across 238 hospitals in 66 countries (91 hospitals in 20 high-income countries; 57 hospitals in 19 upper-middle-income countries; and 90 hospitals in 27 low-income to lower-middle-income countries). The availability of five hospital facilities was inversely associated with mortality: ultrasound, CT scanner, critical care unit, opioid analgesia, and oncologist. After adjustment for case-mix and country income group, hospitals with three or fewer of these facilities (62 hospitals, 1294 patients) had higher mortality compared with those with four or five (adjusted odds ratio [OR] 3·85 [95% CI 2·58–5·75]; p<0·0001), with excess mortality predominantly explained by a limited capacity to rescue following the development of major complications (63·0% vs 82·7%; OR 0·35 [0·23–0·53]; p<0·0001). Across LMICs, improvements in hospital facilities would prevent one to three deaths for every 100 patients undergoing surgery for cancer. Interpretation: Hospitals with higher levels of infrastructure and resources have better outcomes after cancer surgery, independent of country income. Without urgent strengthening of hospital infrastructure and resources, the reductions in cancer-associated mortality associated with improved access will not be realised. Funding: National Institute for Health and Care Research