Accessible opera : overcoming linguistic and sensorial barriers

The desire to make media available for all has been rapidly accepted and implemented by most European countries. Opera, as one of the many audiovisual representations, also falls under the category of production which needs to be made accessible and this article aims to analyse how opera has gone through a complete transformation to become a cultural event for all, overcoming not only linguistic but also sensorial barriers. The first part of the article analyses the various forms of translation associated with opera and the main challenges they entail. The second presents different systems used to make opera accessible to the sensorially challenged, highlighting their main difficulties. Examples from research carried out at the Barcelona's Liceu opera house are presented to illustrate various modalities, especially audio description. All in all, it is our aim to show how translated-related processes have made it possible to open opera to a wider audience despite some initial reluctance

Variations on the Pear Tree Experiment : different variables, new results?

Inspired by the Pear Stories Project, the Pear Tree Project has investigated how different cultures and languages describe the same film in order to apply its findings to audio description (AD). Participants from different countries were asked to "write down what they saw" in a controlled setting. This article proposes an alternative experiment, also based on the original Pear Stories Project, which aims to shed light on two issues: how different describer profiles (translation students with AD training/without AD training) and different instructions concerning the target audience profiles (blind/non-blind) could alter the final production. The results are analysed in this paper, taking into account the elements covered in the original Pear Stories Project as well as some additional elements proposed by the authors

Differentiating lower motor neuron syndromes

Lower motor neuron (LMN) syndromes typically present with muscle wasting and weakness and may arise from pathology affecting the distal motor nerve up to the level of the anterior horn cell. A variety of hereditary causes are recognised, including spinal muscular atrophy, distal hereditary motor neuropathy and LMN variants of familial motor neuron disease. Recent genetic advances have resulted in the identification of a variety of disease-causing mutations. Immune-mediated disorders, including multifocal motor neuropathy and variants of chronic inflammatory demyelinating polyneuropathy, account for a proportion of LMN presentations and are important to recognise, as effective treatments are available. The present review will outline the spectrum of LMN syndromes that may develop in adulthood and provide a framework for the clinician assessing a patient presenting with predominantly LMN features

Analysis of age-related left ventricular collagen remodeling in living donors: Implications in arrhythmogenesis

Age-related fibrosis in the left ventricle (LV) has been mainly studied in animals by assessing collagen content. Using second-harmonic generation microscopy and image processing, we evaluated amount, aggregation and spatial distribution of LV collagen in young to old pigs, and middle-age and elder living donors. All collagen features increased when comparing adult and old pigs with young ones, but not when comparing adult with old pigs or middle-age with elder individuals. Remarkably, all collagen parameters strongly correlated with lipofuscin, a biological age marker, in humans. By building patient-specific models of human ventricular tissue electrophysiology, we confirmed that amount and organization of fibrosis modulated arrhythmia vulnerability, and that distribution should be accounted for arrhythmia risk assessment. In conclusion, we characterize the age-associated changes in LV collagen and its potential implications for ventricular arrhythmia development. Consistency between pig and human results substantiate the pig as a relevant model of age-related LV collagen dynamics. © 2022 The Author(s

An interlaboratory comparison of mid-infrared spectra acquisition: Instruments and procedures matter

Diffuse reflectance spectroscopy has been extensively employed to deliver timely and cost-effective predictions of a number of soil properties. However, although several soil spectral laboratories have been established worldwide, the distinct characteristics of instruments and operations still hamper further integration and interoperability across mid-infrared (MIR) soil spectral libraries. In this study, we conducted a large-scale ring trial experiment to understand the lab-to-lab variability of multiple MIR instruments. By developing a systematic evaluation of different mathematical treatments with modeling algorithms, including regular preprocessing and spectral standardization, we quantified and evaluated instruments' dissimilarity and how this impacts internal and shared model performance. We found that all instruments delivered good predictions when calibrated internally using the same instruments' characteristics and standard operating procedures by solely relying on regular spectral preprocessing that accounts for light scattering and multiplicative/additive effects, e.g., using standard normal variate (SNV). When performing model transfer from a large public library (the USDA NSSC-KSSL MIR library) to secondary instruments, good performance was also achieved by regular preprocessing (e.g., SNV) if both instruments shared the same manufacturer. However, significant differences between the KSSL MIR library and contrasting ring trial instruments responses were evident and confirmed by a semi-unsupervised spectral clustering. For heavily contrasting setups, spectral standardization was necessary before transferring prediction models. Non-linear model types like Cubist and memory-based learning delivered more precise estimates because they seemed to be less sensitive to spectral variations than global partial least square regression. In summary, the results from this study can assist new laboratories in building spectroscopy capacity utilizing existing MIR spectral libraries and support the recent global efforts to make soil spectroscopy universally accessible with centralized or shared operating procedures

Altered Prion Protein Expression Pattern in CSF as a Biomarker for Creutzfeldt-Jakob Disease

Creutzfeldt-Jakob disease (CJD) is the most frequent human Prion-related disorder (PrD). The detection of 14-3-3 protein in the cerebrospinal fluid (CSF) is used as a molecular diagnostic criterion for patients clinically compatible with CJD. However, there is a pressing need for the identification of new reliable disease biomarkers. The pathological mechanisms leading to accumulation of 14-3-3 protein in CSF are not fully understood, however neuronal loss followed by cell lysis is assumed to cause the increase in 14-3-3 levels, which also occurs in conditions such as brain ischemia. Here we investigated the relation between the levels of 14-3-3 protein, Lactate dehydrogenase (LDH) activity and expression of the prion protein (PrP) in CSF of sporadic and familial CJD cases. Unexpectedly, we found normal levels of LDH activity in CJD cases with moderate levels of 14-3-3 protein. Increased LDH activity was only observed in a percentage of the CSF samples that also exhibited high 14-3-3 levels. Analysis of the PrP expression pattern in CSF revealed a reduction in PrP levels in all CJD cases, as well as marked changes in its glycosylation pattern. PrP present in CSF of CJD cases was sensitive to proteases. The alterations in PrP expression observed in CJD cases were not detected in other pathologies affecting the nervous system, including cases of dementia and tropical spastic paraparesis/HTLV-1 associated myelopathy (HAM/TSP). Time course analysis in several CJD patients revealed that 14-3-3 levels in CSF are dynamic and show a high degree of variability during the end stage of the disease. Post-mortem analysis of brain tissue also indicated that 14-3-3 protein is upregulated in neuronal cells, suggesting that its expression is modulated during the course of the disease. These results suggest that a combined analysis of 14-3-3 and PrP expression pattern in CSF is a reliable biomarker to confirm the clinical diagnosis of CJD patients and follow disease progression

The Roadmap to the POEMMA mission

The Probe Of Extreme Multi-Messenger Astrophysics (POEMMA) is designed to observe ultrahigh-energy cosmic rays (UHECRs) and cosmic neutrinos from space with sensitivity over the full celestial sky. Developed as a NASA Astrophysics Probe-class mission, POEMMA consists of two identical telescopes orbiting the Earth in a loose formation designed to observe extensive air showers (EAS) via air fluorescence and Cherenkov emissions. UHECRs and UHE neutrinos above 20 EeV are observed with the stereo fluorescence technique, while tau neutrinos above 20 PeV are observed via the optical Cherenkov signals produced by up-going EAS generated by the decay of Earth-emerging tau-leptons. The POEMMA satellites are designed to quickly re-orientate to follow up transient cosmic neutrino candidate sources and obtain unparalleled neutrino flux sensitivity. Both observation techniques and the instrument design are being validated by current and upcoming missions, such as Mini-EUSO and EUSO-SPB as part of the JEM-EUSO program, and the Terzina instrument onboard the NUSES SmallSat mission. We discuss the POEMMA science performance and the current roadmap to the POEMMA mission

Prospects for Cross-correlations of UHECR Events with Astrophysical Sources with Upcoming Space-based Experiments

Ultra-high energy cosmic rays (UHECRs) are the messengers of the most extreme physics in the cosmos; however, efforts to identify their origins have thus far been thwarted by the fact that they don’t point back to their sources. Using statistical studies cross-correlating UHECR arrival directions with astrophysical catalogs, the ground-based Pierre Auger Observatory has reported hints of a correlation with nearby starburst galaxies, as well as lower-significance correlations with other classes of astrophysical sources. Space-based UHECR experiments, such as POEMMA and ZAP, will monitor large interaction volumes on the Earth or the Moon. Within a few years of mission operation time, both missions will achieve unprecedented exposures at energies above 40 EeV across the entire sky. We present studies of the cross-correlation between UHECR event arrival directions and astrophysical catalogs as motivated by expectations for the detector performance for POEMMA and ZAP. We find that both POEMMA and ZAP will achieve 5σ discovery reach for many plausible astrophysical scenarios