1,813 research outputs found
Kemalangan jalanraya
Kemalangan jalanraya adalah disebabkan oleh beberapa faktor yang merupakan isu serius pada masa ini. Di antara sebab-sebab utama meningkatnya jumlah kemalangan jalanraya di Malaysia adalah kerana jumlah isipadu lalulintas yang tinggi. pada tahun 1983 didapati bilangan kemalangan jalanraya telah meningkat sebanyak 8%. Jumlah kematian adalah 3,220 orang dari jumlah keseluruhan 72,634 kes kemalangan.
There are numerous factors responsible for road accidents, which are now causing serious issue. Among the reasons giving rise to the road accident in Malaysia are high traffic volume. It has been revealed that the number of accidents that is 72,634 cases, the death toll is 3,220 cases
Warga UMP berkayak Sehati Sejiwa
Bersempena sambutan Bulan Kebangsaan dan Hari Malaysia, Universiti Malaysia Pahang (UMP) baru-baru ini menganjurkan program Berkayak Sehati Sejiwa sambil mengibarkan Jalur Gemilang mengelilingi tasik di universiti berkenaan di kampus Pekan di sini
Cabaran kaki pancing UMP
PERTANDINGAN Memancing Terbuka sempena Ekspokonvo Universiti Malaysia Pahang (UMP) di tasik universiti berkenaan di Pekan, Pahang baru-baru ini berlangsung meriah dengan penyertaan hampir 600 kaki pancing
The development of style in Malaysian printmaking: 1930-2000 / Mohd Jamil Mat Isa
The development of printmaking started as a medium of communication, be it a religious propaganda or as a tool to achieve independence. Printmaking can be classified into several categories, such as relief printing, intaglio, planographic and now in the modern era, is a digital print. Malaysian printmaking has been developing since the Colonial Era (1930s) by Chuah Thien Teng and Abdullah Ariff. Woodcut and linocut are the techniques chosen by them. Later on, other artists explored the medium; they applied more techniques included the latest digital technology. The objectives of this study are to trace on the development of style in Malaysian printmaking, to identify the factors that influence the development of style in Malaysian printmaking and to document the historical development of Malaysian printmaking. This research will use qualitative methods with art historical approach based on the primary and secondary data. The data collected in the form of text from books, exhibition catalogues, and art auction catalogues. The works of printmaking are divided into two main categories, namely the Pre-Independence Era (1930-1957) and Post-Independence Era (1958-2000)
Molecular Characterisation Of Infectious Bursal Disease Virus And Expression Of Vp2 Protein For The Development Of Diagnostic Kit And Recombinant Vaccine
Outbreak of infectious bursal disease (IBD) in chickens due to highly pathogenic strain of IBD virus (vvIBDV) was first reported in Europe in late 1980’s and in Malaysia in 1991. The disease caused significant economic losses, estimated more than RM72 million per year in Malaysia alone due to high mortality and immunosuppression. Treatment of IBD is of no value and the disease can only be controlled and prevented by proper vaccination programme and biosecurity. It was the objectives of the study to determine the molecular characteristic of Malaysian field isolates of IBDV and expression of the VP2 gene of the isolate for the development of diagnostic kit and recombinant vaccine. Three IBDV isolates identified as UPM04178, UPM04190 and UPM04238 were characterised. Based on their pathogenicity and sequence characteristic, the highest similarity (98%) concerning both nucleotide and amino acid sequences, the IBDV isolates were characterized as vvIBDV strains. Evolutionary relatedness of the isolates to vvIBDV strains was demonstrated by three phylogenetic methods: bootstrap values of 100%, 95% and 90% for nucleotide sequences and those of 58%, 86% and 96% for amino acid sequences were obtained by the distance, maximum parsimony and maximum likehood methods, respectively. Phylogenetic analysis revealed clustering of the isolates with vvIBDV strains of serotype 1, which originate from a common ancestor of IBDV strains present in Malaysia.
Using informative characteristics of the isolate, both diagnostic kit and recombinant vaccine were successfully developed using a new wild-type field vvIBDV strain of UPM04190 isolate. A safe and effective recombinant IBD vaccine was developed base on the construction of recombinant VP2 gene of the isolate cloned into an Escherichia coli expression system. The VP2 gene was inserted into pRSET B vector as a fusion protein with histidine tag, which can be easily purified. The recombinant VP2 protein bands were expressed to their expected sizes of ~50 kDa from cell lysate. The pRSET vectors are pUC-derived expression vectors and expression of the gene of interest from pRSET is controlled by the strong phage T7 promoter that drives expression of gene 10 (Φ10) which provides protein stability and help to maintain the original structure of the protein. High-level production (3 mg/ml) of soluble product of VP2 recombinant protein was achieved with modified techniques of expression conditions and approaches. Efficacy test demonstrated that the recombinant vaccine of various fractions could provide protection ranging from 75% to 100% in highly susceptible chickens (specific pathogen free chickens) when challenged with vvIBDV (B00/81) at 104.25 EID50/ml per chicken following vaccination. One-step-immunostrip kit which is highly specific and sensitive was developed using whole virus as capture antigen and high-affinity polyclonal IBD antibodies coated with gold particles. Rapid detection of IBD antibody can be achieved as fast as two minutes in a clinical or field environment. The kit is highly sensitive as it can detect as low as 250 ELISA units compared to commercial ELISA kit that only goes to 391 ELISA units for positive samples. The specificity of the kit was evaluated against antibody of other chicken viruses. No signal of reactivity or cross react exists among the antibodies tested. Thus, it was highly specific to IBDV. It was concluded that the local IBDV isolates were proven to be vvIBDV strain, the constructed recombinant vaccine provide a safe and effective protection and, the developed one-step-immunostrip kit is rapid, specific, sensitive, safe and economic in detection of IBDV infection and monitoring immune status of chicken against IBD
DNA Variations Within Yam Cultivars (Dioscorea Spp) as Indicated by RAPD Analysis
A research on the DNA variations in eighteen yam cultivars from Malaysia and
Japan was conducted using RAPD method in order to identify the cultivars and
evaluate their genetic relationship. A preliminary identification of the cultivars
was made using morphological characters of the plants. Ten primers, which
were capable of producing PCR amplifications, were used in this research and
were able to produce 515 amplification bands.
Primer OPG-13 was found to be the best primer producing the most number
and unambiguous fragments. All the ten primers were used for identifying and
elucidating the genetic relationship among the cultivars. The dendrogram
produced from the NTsys programme based on UPGMA, by using RAPD data showed that a close genetic relationship with a high percentage of similarity is
between accession UPM DA 023 with accession UPM DA 024. Accession
UPM DE 007 is a different species that is of Disocorea esculenta and thus
genetically distant from the other accessions of Dioscorea alata. The tuber and
leaf morphology of D. esculenta is also different from those of Dioscorea alata
and indeed their genome is different too.
The results of this research show that the RAPD technique can be used to
identify the yam cultivars and to determine the genetic relationship among the
cultivars. The genotype of all cultivars from Japan can be identified using only
one primer, which is primer OPG-13. On the other hand, the identification of
cultivars from Malaysia requires four primers, which are OPB-07, OPB-15,
OPG-02 and OPG-13.
In general, there is a close genetic relationship among yam cultivars found in
Malaysia. However, these cultivars are different from Japan cultivars with the
similarity index as low as 31.3%, indicating a distant relationship between them.
RAPD is a very useful technique for the determination of genetic relationship
and identification of plant cultivars. Such technique was successfully employed
in genetic relationship and identification studies of D. alata and D. esculenta
cultivars in Malaysia
SL1M-IBE TNB pacu keupayaan graduan
Graduan Ijazah Sarjana Muda Pengurusan Sumber Manusia dari Universiti Teknologi Mara (UiTM), Nurfadzidah Azhar, 24, tidak menyangka keputusannya untuk menghadiri temuduga terbuka Skim Latihan 1Malaysia - Industri Bekalan Elektrik (SL1M-IBE) kelolaan Tenaga Nasional Berhad (TNB) telah membantu melengkapkan dirinya untuk memasuki dunia pekerjaan
Tiga sempel protein dibawa ke ISS
Tiga sampel protein yang dihasilkan seorang saintis tempatan bakal dibawa ke Stesen Angkasa Lepas Antarabangsa (ISS) menerusi kapal angkasa milik Russia, 'Soyuz' September ini
A foresight study on green technology in paint industries in Malaysia
Green technology paint industries have been rising significantly over the years due to its benefit to environment and consequently to human health. However, the high manufacturing cost and other disadvantages threatened its sustainability in the future. Therefore a research was carried out to identify the issues and drivers of employing in green technology paint industries and to study the future trend of green technology paints in Malaysia. STEEPV approach which is an acronym for (Social, Technological, Environmental, Economic, Political and Values) is an internationally recognized tool used in conducting technology foresight worldwide. This tool was used in the current research in identifying various issues and drivers based on the above mentioned six categories. New technologies in preparing low energy consuming paints and the role of government and non-governmental organization was voted as the most influential drivers for the eco-friendly paint industries in Malaysia. In line with this regard, a scenario development carried out and a number of projects were suggested in order to move towards sustainable development of eco-friendly paint industry. As a consequence it is recommended to add more political will and support in such projects to ensure the ownership at the top level and maximize the chances of implementing the outcomes
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