192 research outputs found
Attachment to an endogenous laminin-like protein initiates sprouting by leech neurons.
Leech neurons in culture sprout rapidly when attached to extracts from connective tissue surrounding the nervous system. Laminin-like molecules that promote sprouting have now been isolated from this extracellular matrix. Two mAbs have been prepared that react on immunoblots with a approximately equal to 220- and a approximately equal to 340-kD polypeptide, respectively. These antibodies have been used to purify molecules with cross-shaped structures in the electron microscope. The molecules, of approximately equal to 10(3) kD on nonreducing SDS gels, have subunits of approximately equal to 340, 220, and 160-180 kD. Attachment to the laminin-like molecules was sufficient to initiate sprouting by single isolated leech neurons in defined medium. This demonstrates directly a function for a laminin-related invertebrate protein. The mAbs directed against the approximately equal to 220-kD chains of the laminin-like leech molecule labeled basement membrane extracellular matrix in leech ganglia and nerves. A polyclonal antiserum against the approximately equal to 220-kD polypeptide inhibited neurite outgrowth. Vertebrate laminin did not mediate the sprouting of leech neurons; similarly, the leech molecule was an inert substrate for vertebrate neurons. Although some traits of structure, function, and distribution are conserved between vertebrate laminin and the invertebrate molecule, our results suggest that the functional domains differ
Nuclear dependence of the transverse-single-spin asymmetry for forward neutron production in polarized collisions at GeV
During 2015 the Relativistic Heavy Ion Collider (RHIC) provided collisions of
transversely polarized protons with Au and Al nuclei for the first time,
enabling the exploration of transverse-single-spin asymmetries with heavy
nuclei. Large single-spin asymmetries in very forward neutron production have
been previously observed in transversely polarized collisions at
RHIC, and the existing theoretical framework that was successful in describing
the single-spin asymmetry in collisions predicts only a moderate
atomic-mass-number () dependence. In contrast, the asymmetries observed at
RHIC in collisions showed a surprisingly strong dependence in
inclusive forward neutron production. The observed asymmetry in Al
collisions is much smaller, while the asymmetry in Au collisions is a
factor of three larger in absolute value and of opposite sign. The interplay of
different neutron production mechanisms is discussed as a possible explanation
of the observed dependence.Comment: 315 authors, 8 pages, 4 figures, 1 table. v2 is version accepted for
publication in Phys. Rev. Lett. Plain text data tables for the points plotted
in figures for this and previous PHENIX publications are (or will be)
publicly available at http://www.phenix.bnl.gov/papers.htm
L\'evy-stable two-pion Bose-Einstein correlations in GeV AuAu collisions
We present a detailed measurement of charged two-pion correlation functions
in 0%-30% centrality GeV AuAu collisions by the
PHENIX experiment at the Relativistic Heavy Ion Collider. The data are well
described by Bose-Einstein correlation functions stemming from L\'evy-stable
source distributions. Using a fine transverse momentum binning, we extract the
correlation strength parameter , the L\'evy index of stability
and the L\'evy length scale parameter as a function of average
transverse mass of the pair . We find that the positively and the
negatively charged pion pairs yield consistent results, and their correlation
functions are represented, within uncertainties, by the same L\'evy-stable
source functions. The measurements indicate a decrease of the
strength of the correlations at low . The L\'evy length scale parameter
decreases with increasing , following a hydrodynamically
predicted type of scaling behavior. The values of the L\'evy index of stability
are found to be significantly lower than the Gaussian case of
, but also significantly larger than the conjectured value that may
characterize the critical point of a second-order quark-hadron phase
transition.Comment: 448 authors, 25 pages, 11 figures, 4 tables, 2010 data. v2 is version
accepted for publication in Phys. Rev. C. Plain text data tables for the
points plotted in figures for this and previous PHENIX publications are (or
will be) publicly available at http://www.phenix.bnl.gov/papers.htm
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