Biosynthesis of tetrahydrofuran lignans in Virola surinamensis

Através de experimentos de administração in vivo em plântulas de Virola surinamensis, observaram-se as incorporações de fenilalanina e do fenilpropanóide E-isoeugenol no 4-O-metil-5-metoxi-E-isoeugenol (E-isoelemicina) e na lignana tetraidrofurânica verrucosina.The labelled substrates phenylalanine and phenylpropanoid E-isoeugenol were incorporated to 5-methoxy-4-O-methyl-E-isoeugenol (E-isoelemicin) and to the tetrahydrofuran lignan verrucosin in plantlets of Virola surinamensis (Myristicaceae)

Portrait of an act: Aesthetics and ethics in The 'Portrait of a Lady' (Henry James)

Five tetrahydrofuran lignans and two known flavones were isolated from the aerial parts of Peperomia blanda. The structures of the isolated lignans were elucidated by interpretation of their spectroscopic data, including by gHMQC and gHMBC. The relative and absolute configurations of the isolates were determined from NOESY interactions and optical properties, respectively. Four of the lignans were diastercomeric whilst one was of mixed biosynthetic origin. All but one of the lignans exhibited high in vitro trypanocidal activity when assayed against epimastigotes of Trypanosoma cruzi strain Y. (C) 2007 Elsevier Ltd. All rights reserved

Uptake of Seeds Secondary Metabolites by Virola surinamensis Seedlings

The major secondary metabolites and fatty acids occurring in the seeds of Virola surinamensis were monitored by GC-MS during germination and seedling development. The role as carbon source for seedling development was indicated considering that both classes of compounds were similarly consumed in the seeds and that no selective consumption of compounds could be detected

Interaction Gardens and Butterfly Catalogues: a Joint Strategy to Promote Capacity Development in Protected Areas and Reduce the Extinction of Experience in Cities

More than half of the world’s population live in cities. Increasing numbers of generations are now born and raised in urban landscapes with decreasing opportunities towards interacting with natural environments. This extinction of experience leads to environmental apathy and lack of bioliteracy, which is a central aspect to be tackled in conservation strategies. At the same time, people who live near or at non-urban settings are closer to natural habitats but do not have similar access to concepts of environmentalism and may lack incentives to lean towards nature conservation, instead of its exploitation or even illegal activities. We here propose that interaction gardens, that is, gardens with multiple trophic levels (primary producers, herbivores, predators, and parasitoids), can tackle these issues, especially if planned with incentives, such as butterfly catalogues, aimed as a common goal of the garden community. It can also bring benefits to human health and well-being, increase the survival chances of local biodiversity, and strengthen the front line of conservation by promoting income strategies to people who live near protected areas. Our specific aims are to 1) share a case study of capacity development at the Intervales State Park in the Atlantic Forest, Southeast Brazil; 2) present the butterfly catalogue of this protected area; 3) present guidelines for interaction gardens at both urban and non-urban settings; and 4) discuss alternative perspectives about Neotropical conservation. We provide a translated version of the text in Portuguese to encourage students, educators, NGOs and local communities of other protected areas to venture in our proposed joint strategy of interaction gardens with butterfly catalogues

Producción de metabolitos secundarios en cultivo de raíces in vitro y suspensiones celulares de Ipomoea carnea spp. carnea Jacq.

Ipomoea carnea spp. carnea Jacq. es un arbusto dominante en el bosque estacionalmente seco del Perú y Ecuador. Los cultivos de raíces fueron establecidos en medio MS con diferentes concentra- ciones de AIB, sacarosa y combinaciones de AIB-sacarosa. Diver- sos explantes obtenidos de plántulas in vitro y plantas silvestres se cultivaron en diferentes combinaciones de 2,4-D, AIA, ANA y BAP, para inducir callos friables, dependiendo del tipo de explante y los reguladores de crecimiento. Suspensiones celulares se establecie- ron a partir de callos y estudiaron en su crecimiento y acumulación de metabolitos secundarios. Los metabolitos secundarios identifi- cados en cultivo de raíces y suspensiones celulares fueron feno- les, diversos flavonoides y la cumarina escopoletina

Grandisin induces apoptosis in leukemic K562 cells

In this study, the potential antileukemic activity of grandisin, a lignan extracted from Piper solmsianum, was evaluated against the leukemic line K562. The cytotoxicity of grandisin (0.018 to 2.365 µM) was evaluated in K562 and normal peripheral blood lymphocytes by Trypan Blue Exclusion and MTT methods after 48h exposure to the drug. In both methods, cellular viability was concentration-dependent and the IC50 values were lower than 0.85µM. Analysis of K562 cells after treatment with grandisin showed that the cell cycle was arrested in the G1 phase with a 12.31% increase, while both S and G2 phases decreased. Morphological studies conducted after the exposure of K562 to grandisin revealed changes consistent with the apoptosis process, which was confirmed by anexin V stain and caspase activation. Thus, lignan grandisin showed antileukemic activities against the K562 cell line and the cell death process occurred via apoptosis

He reform of theHungarian higher education and the non-university sector

One of the most important weak points of the Hungarian higher education (just like the Central European higher education system) in the communist era was the extremely low access rate, roughly around 10% of the age group in the region. Such a system cuts the way before a large percentage of the age group towards post-secondary education and we cannot help thinking about political considerations of the governing nomenclature behind it. In some countries of the region the situation could be qualified even anticonstitutional since the respective constitutions stipulated: Each citizen, having graduated from the secondary education system has the right to enter the post-secondary education in a filiere appropriately chosen according to his or her capacities. Beyond this bottleneck at the access to higher education another common characteristic of the region higher education system were the inappropriate, if existing, links between education and economy. This bad relationship is especially dangerous in a period of rapid economical and social changes of the society. Regardless of the direction of the changes, the accelerated evolution of the society necessitates a high level of adaptability of the education and training system. Traditional higher education systems with their long cycle courses - Iet alone the sometimes obsolete curricula - are not able to satisfy the rapidly appearing needs of the new economical system. ln the same time on the traditional fields the good performance of the higher educational systems was weIl known. This gave the false justification for the higher education institutions to preserve their structure, profile, curricula, etc

Larvicidal activity of Piper tuberculatum on Spodoptera frugiperda (Lepidoptera: Noctuidae) under laboratory conditions

The larvicidal activity of the neotropical "matico" Piper tuberculatum was evaluated. The secondary compounds were extracted of leaves, stems and mature spikes with fruits and seeds from wild plants and in vitro plants of Piper tuberculatum. The acute toxicities to the fall armyworm, Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae), of extracts of spikes with fruits and seeds and in vitro plants of P tuberculatum were evaluated by means of contact bioassays. Only CH2Cl2:MeOH (2:1) and EtOH extracts of mature spikes and CH2C12:MeOH (2:1) extract from in vitro plants showed significant levels of larval mortality. The CH2Cl2:MeOH (2:1) and EtOH extracts of mature spikes caused 90% mortality when doses of 0.1850 mg/mu L were applied to the S. frugiperda in 24 and 48 h of exposure, respectively. The CH2Cl2:MeOH (2:1) extract from in vitro plants caused 95% mortality when doses of 0.1850 mg/mu L were too applied in 48 h of exposure. The mature spikes test best results were: LD50 0.001 mg/mu L with EtOH and 0.007 mg/mu L with CH2Cl2:MeOH (2:1) and LD90 0.027 mg/mu L with EtOH and 0.103 mg/mu L with CH2Cl2:MeOH (2:1); and, in the case of in vitro plants, only CH2Cl2:MeOH (2:1) extract was: LD50 0.003 mg/mu L and LD90 0.060 mg/mu L. The potential value of extracts derived from P. tuberculatum as efficient insecticides against S.frugiperda is discussed

Plasticity in plant defense and the role of phytochemical dissimilarity in limiting specialist herbivory

Phytochemical diversity is an effective plant defensive attribute, but much more research has focused on genetic and environmental controls of specific defensive compounds than phytochemical diversity per se. Documenting plasticity in phytochemical richness and plant chemical composition as opposed to individual compounds is important for understanding plant defense. This study outlines a multi-site transplant experiment in Cerrado gallery forests in central Brazil, utilizing Piper arboreum (Piperaceae), a prevalent and widespread neotropical shrub. Clones from four distinct populations were planted either at their origin site or in a different forest. Secondary metabolite composition varied between populations initially and then changed after transplanting. Interestingly, clones with chemical profiles that were distinct from the populations where they were introduced experienced reduced specialist chrysomelid herbivory compared to clones that were more chemically similar to the existing P. arboreum populations where they were planted. Specialist Lepidoptera herbivory also declined in clones transplanted to a new forest, but this change could not be ascribed to chemical profiles. In contrast, generalist herbivory was unaffected by chemical dissimilarity and transplanting. This research adds to the expanding body of evidence suggesting that phytochemical diversity is a dynamic trait exerting unique effects on different herbivore guilds