Desiccation tolerance of Botryococcus braunii (Trebouxiophyceae, Chlorophyta) and extreme temperature tolerance of dehydrated cells

Botryococcus braunii Kützing, a green colonial microalga, occurs worldwide in both freshwater and brackish water environments. Despite considerable attention to B. braunii as a potential source of renewable fuel, many ecophysiological properties of this alga remain unknown. Here, we examined the desiccation and temperature tolerances of B. braunii using two newly isolated strains BOD-NG17 and BOD-GJ2. Both strains survived through 6- and 8-month desiccation treatments but not through a 12-month treatment. Interestingly, the desiccation-treated cells of B. braunii gained tolerance to extreme temperature shifts, i.e., high temperature (40 °C) and freezing (−20 °C). Both strains survived for at least 4 and 10 days at 40 and −20 °C, respectively, while the untreated cells barely survived at these temperatures. These traits would enable long-distance dispersal of B. braunii cells and may account for the worldwide distribution of this algal species. Extracellular substances such as polysaccharides and hydrocarbons seem to confer the desiccation tolerance

A Suzaku Observation of the Low-Ionization Fe-Line Emission from RCW 86

The newly operational X-ray satellite Suzaku observed the southwestern quadrant of the supernova remnant (SNR) RCW 86 in February 2006 to study the nature of the 6.4 keV emission line first detected with the Advanced Satellite for Cosmology and Astronomy (ASCA). The new data confirm the existence of the line, localizing it for the first time; most of the line emission is adjacent and interior to the forward shock and not at the locus of the continuum hard emission. We also report the first detection of a 7.1 keV line that we interpret as the K-beta emission from low-ionization iron. The Fe-K line features are consistent with a non-equilibrium plasma of Fe-rich ejecta with n_{e}t <~ 10^9 cm^-3 s and kT_{e} ~ 5 keV. This combination of low n_{e}t and high kT_{e} suggests collisionless electron heating in an SNR shock. The Fe K-alpha line shows evidence for intrinsic broadening, with a width of 47 (34--59) eV (99% error region). The difference of the spatial distributions of the hard continuum above 3 keV and the Fe-K line emission support a synchrotron origin for the hard continuum.Comment: 6 pages with 6 figures. Accepted for PASJ Suzaku Special Issue (vo. 58, sp.1

Fyn phosphorylates AMPK to inhibit AMPK activity and AMP-dependent activation of autophagy

We previously demonstrated that proto-oncogene Fyn decreased energy expenditure and increased metabolic phenotypes. Also Fyn decreased autophagy-mediated muscle mass by directly inhibiting LKB1 and stimulating STAT3 activities, respectively. AMPK, a downstream target of LKB1, was recently identified as a key molecule controlling autophagy. Here we identified that Fyn phosphorylates the α subunit of AMPK on Y436 and inhibits AMPK enzymatic activity without altering the assembly state of the AMPK heterotrimeric complex. As pro-inflammatory mediators are reported modulators of the autophagy processes, treatment with the pro-inflammatory cytokine TNFα resulted in 1) increased Fyn activity 2) stimulated Fyn-dependent AMPKα tyrosine phosphorylation and 3) decreased AICAR-dependent AMPK activation. Importantly, TNFα induced inhibition of autophagy was not observed when AMPKα was mutated on Y436. 4) These data demonstrate that Fyn plays an important role in relaying the effects of TNFα on autophagy and apoptosis via phosphorylation and inhibition of AMPK

Effectiveness of Anti-PD-1 Antibody Monotherapy for the Primary Malignant Melanoma of the Esophagus： A Case Report

Primary malignant melanoma of the esophagus（PMME）is extraordinarily rare with a high degree of malignancy and poor prognosis, and a standard therapy remains to be established. The anti-PD-1 antibody nivolumab is a promising agent for various cancers. To our knowledge, this is the first case report of PMME where a complete response was achieved using nivolumab. We report an 80-year-old woman who was diagnosed with PMME with bone metastasis and lymph node metastases. Although dacarbazine combined chemotherapy was performed and continued for six cycles, the primary tumor progressed and liver metastases appeared. The patient then received nivolumab monotherapy. After three cycles, nivolumab monotherapy for PMME resulted in a complete response as shown by positron emission tomography, computed tomography, and esophagogastroduodenoscopy. In our case, nivolumab exerted a curative effect on PMME, thus suggesting that nivolumab can be effective in the treatment of this rare disease

Time course of discordant BMIPP and thallium uptake after ischemia and reperfusion in a rat model

金沢大学大学院医学系研究科Serial changes in fatty acid metabolism or use associated with acute ischemia and reperfusion were examined in rat hearts. Methods: Male Wistar rats were subjected to occlusion of the left coronary artery for 20 min followed by reperfusion. After release of the occlusion, groups of animals were allowed to recover for intervals of 20 min (n = 9), 1 d (n = 9), 3 d (n = 6), 7 d (n = 6), or 30 d (n = 6). Hearts were excised 15-20 min after injection of 0.74 MBq of 125I-15-(p-iodophenyl)-3-R,S-methylpentadecanoic acid (BMIPP) and 14.8 MBq of 201TI. One minute before resection, the left coronary artery was reoccluded and 185 MBq of 99mTc-sestamibi were injected to document the area at risk. Triple-tracer autoradiography was performed to assess tracer uptake. Uptake ratios of BMIPP and 201TI in the area at risk were calculated on the basis of the count density in the lesion divided by that in the normally perfused area. Results: 201TI uptake did not change throughout the observation period (P = 0.25). In contrast, BMIPP uptake increased early in the acute phase (20 min and 1 d), decreased during the sub-acute phase (7 d), and subsequently recovered in the chronic phase (30 d). Conclusion: The present investigation clearly illustrated that BMIPP uptake is higher than 201TI uptake in the acute phase, that BMIPP uptake is lower than 201TI uptake in the subacute phase, and that BMIPP uptake and 201TI uptake are similar in the chronic phase. These results yield data essential to the precise interpretation of BMIPP images

Contribution of Thyrotropin-Releasing Hormone to Cerebellar Long-Term Depression and Motor Learning

Thyrotropin-releasing hormone (TRH) regulates various physiological activities through activation of receptors expressed in a broad range of cells in the central nervous system. The cerebellum expresses TRH receptors in granule cells and molecular layer interneurons. However, the function of TRH in the cerebellum remains to be clarified. Here, using TRH knockout (KO) mice we studied the role of TRH in the cerebellum. Immunohistochemistry showed no gross morphological differences between KO mice and wild-type (WT) littermates in the cerebellum. In the rotarod test, the initial performance of KO mice was comparable to that of WT littermates, but the learning speed of KO mice was significantly lower than that of WT littermates, suggesting impaired motor learning. The motor learning deficit in KO mice was rescued by intraperitoneal injection of TRH. Electrophysiology revealed absence of long-term depression (LTD) at parallel fiber-Purkinje cell synapses in KO mice, which was rescued by bath-application of TRH. TRH was shown to increase cyclic guanosine monophosphate (cGMP) content in the cerebellum. Since nitric oxide (NO) stimulates cGMP synthesis in the cerebellum, we examined whether NO-cGMP pathway was involved in TRH-mediated LTD rescue in KO mice. Pharmacological blockade of NO synthase and subsequent cGMP production prevented TRH-induced LTD expression in KO mice, whereas increase in cGMP signal in Purkinje cells by 8-bromoguanosine cyclic 3’,5’-monophosphate, a membrane-permeable cGMP analog, restored LTD without TRH application. These results suggest that TRH is involved in cerebellar LTD presumably by upregulating the basal cGMP level in Purkinje cells, and, consequently, in motor learning

X-Ray Spectroscopy of SN 1006 with Suzaku

We report on observations of SN 1006 with the X-ray Imaging Spectrometers (XIS) on board Suzaku. We firmly detected K-shell emission from Fe, for the first time, and find that the Fe ionization state is quite low. The broad band spectrum extracted from the southeast of the remnant is well fitted with a model consisting of three optically thin thermal non-equilibrium ionization plasmas and a power-law component. Two of the thermal models are highly overabundant in heavy elements and, hence, are likely due to ejecta. These components have different ionization parameters: $n_et \sim 1.4\times 10^{10}$ cm$^{-3}$ s and $n_et \sim 7.7\times 10^8$ cm$^{-3}$ s and it is the later one that produces the Fe-K emission. This suggests that Fe has been heated by the reverse shock more recently than the other elements, consistent with a picture where the ejecta are stratified by composition with Fe in the interior. On the other hand, the third thermal component is assumed to be solar abundance, and we associate it with emission from the interstellar medium (ISM). The electron temperature and ionization parameter are $kT_e \sim$0.5 keV and $n_et \sim 5.8\times 10^9$ cm$^{-3}$ s. The electron temperature is lower than that expected from the shock velocity which suggests a lack of collisionless electron heating at the forward shock. The extremely low ionization parameter and extreme non-equilibrium state are due to the low density of the ambient medium.Comment: 12 pages, 9 figures (14 figure files), accepted for publication in PAS

A Suzaku Observation of the Neutral Fe-line Emission from RCW 86

The newly operational X-ray satellite Suzaku observed the supernova remnant (SNR) RCW 86 in February 2006 to study the nature of the 6.4 keV emission line first detected with the Advanced Satellite for Cosmology and Astronomy (ASCA). The new data confirms the existence of the line, localizing it for the first time inside a low temperature emission region and not at the locus of the continuum hard X-ray emission. We also report the first detection of a 7.1 keV line that we interpret as the K(beta) emission from neutral or low-ionized iron. The Fe-K line features are consistent with a non-equilibrium plasma of Fe-rich ejecta with n(sub e) less than or approx. equal to 10(exp 9)/cu cm s and kT(sub e) > 1 keV. We found a sign that Fe K(alpha) line is intrinsically broadened 47 (35-57) eV (99% error region). Cr-K line is also marginally detected, which is supporting the ejecta origin for the Fe-K line. By showing that the hard continuum above 3 keV has different spatial distribution from the Fe-K line, we confirmed it to be synchrotron X-ray emission

Suzaku wide-band observations of SN 1006

We report on the wide band spectra of SN 1006 as observed by Suzaku. Thermal and nonthermal emission are successfully resolved thanks to the excellent spectral response of Suzaku's X-ray CCD XIS. The nonthermal emission cannot be reproduced by a simple power-law model but needs a roll-off at 5.7$\times 10^{16}$ Hz = 0.23 keV. The roll-off frequency is significantly higher in the northeastern rim than in the southwestern rim. We also have placed the most stringent upper limit of the flux above 10 keV using the Hard X-ray Detector.Comment: 16 pages, 8 figures, PASJ, in pres