33 research outputs found
Variableselectioninmultivariatecalibrationbasedonclusteringofvariableconcept
Recentlywehaveproposedanewvariableselectionalgorithm,basedonclusteringofvariableconcept(CLoVA)inclassificationproblem.Withthesameidea,thisnewconcepthasbeenappliedtoaregres-sionproblemandthentheobtainedresultshavebeencomparedwithconventionalvariableselectionstrategiesforPLS.Thebasicideabehindtheclusteringofvariableisthat,theinstrumentchannelsareclusteredintodifferentclustersviaclusteringalgorithms.Then,thespectraldataofeachclusteraresubjectedtoPLSregression.Differentrealdatasets(Cargillcorn,Biscuitdough,ACEQSAR,Soy,andTablet)havebeenusedtoevaluatetheinfluenceoftheclusteringofvariablesonthepredictionper-formancesofPLS.Almostintheallcases,thestatisticalparameterespeciallyinpredictionerrorshowsthesuperiorityofCLoVA-PLSrespecttoothervariableselectionstrategies.Finallythesynergyclusteringofvariable(sCLoVA-PLS),whichisusedthecombinationofcluster,hasbeenproposedasanefficientandmodificationofCLoVAalgorithm.Theobtainedstatisticalparameterindicatesthatvariableclusteringcansplitusefulpartfromredundantones,andthenbasedoninformativecluster;stablemodelcanbereache
Leukemia and small round blue-cell tumor cancer detection using microarray gene expression data set: Combining data dimension reduction and variable selection technique
Using gene expression data in cancer classification plays an important role for solving the fundamental problems
relating to cancer diagnosis. Because of high throughput of gene expression data for healthy and patient samples,
a variable selection method can be applied to reduce complexity of the model and improve the classification
performance. Since variable selection procedures pose a risk of over-fitting, when a large number of variables
with respect to sample are used,we have proposed a method for coupling data dimension reduction and variable
selection in the present study. This approach uses the concept of variable clustering for the original data set.
Significant components of local principal component analysis models have just been retained from all clusters.
Then, the variable selection algorithm is performed on these locally derived principal component variables.
The proposed algorithm has been evaluated on two gene expression data sets; namely, acute Leukemia and
small round blue-cell tumor (SRBCT). Our results confirmed that the classification models achieved on the
reduced data were better than those obtained on the entire microarray gene expression profile
Molecular Dynamics and Docking Investigations of Several Zoanthamine- Type Marine Alkaloids as Matrix Metaloproteinase-1 Inhibitors
Zoanthamine-type alkaloids display a wide spectrum of biological effects. This study aimed
to examine the inhibitory effects of norzoanthamine and its ten homologues of zoanthamine
class on human fibroblast collagenase by modeling a three-dimensional structure of the
ligands at collagenase using energy minimization, docking, molecular dynamics simulation
and MM-PB/GBSA binding free energy calculations. The results showed that zoanthamide,
zooxathellamine and enol-iminium form of norzoanthamine, with lower binding free energies
than other compounds, are potent inhibitors of collagenase. However, the enol-iminium form of
norzoanthamine showed a more inhibitory activity against collagenase than its keto form. This
suggests that it can be used for treatment of many diseases such as osteoporosis, autoimmune
diseases, and cancer. Zinc-binding residues such as His 118, His 122 and His 128 for hydrogen
bonds and Leu 81, Tyr 110, Val 115, Leu 126, Pro 138, Ser 139 for hydrophobic interactions
should be considered for designing an inhibitor for collagenase. Our theoretical results and
MM/GBSA binding free energy calculations are consistent with experimental studies.
Abbreviation
MD: Molecular dynamics; RMSD: Root mean square deviation; MM-PB/GBSA:
Molecular mechanics Poisson-Boltzmann/General Born surface area, DFT: density functional
theory, B3LYP: Becke, three-parameter, Lee-Yang-Parr, RESP: Restrained electrostatic surfacepotentia
Catalytic Cycle of Multicopper Oxidases Studied by Combined Quantum- and Molecular-Mechanical Free-Energy Perturbation Methods.
We have used combined quantum mechanical and molecular mechanical free-energy perturbation methods in combination with explicit solvent simulations to study the reaction mechanism of the multicopper oxidases, in particular, the regeneration of the reduced state from the native intermediate. For 52 putative states of the trinuclear copper cluster, differing in the oxidation states of the copper ions and the protonation states of water- and O2-derived ligands, we have studied redox potentials, acidity constants, isomerization reactions, as well as water- and O2 binding reactions. Thereby, we can propose a full reaction mechanism of the multicopper oxidases with atomic detail. We also show that the two copper sites in the protein communicate so that redox potentials and acidity constants of one site are affected by up to 0.2 V or 3 pKa units by a change in the oxidation state of the other site
SUPPLEMENTARY MATERIAL Marine Natural Products as Acetylcholinesterase Inhibitor: Comparative Quantum Mechanics and Molecular Docking Study
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute,
Bushehr University of Medical Sciences, Bushehr 75147-63448, Ira
Development of a new colorimetric assay for detection of bisphenol-A in aqueous media using green synthesized silver chloride nanoparticles: experimental and theoretical study
In the present study, a cost-effective, green and simple
synthesis method was applied for preparation of stable
silver chloride nanoparticles (AgCl-NPs). The method was
done by forming AgCl-NPs from Ag+ ions using aqueous
extract of brown algae (Sargassum boveanum) obtained from
the Persian Gulf Sea. This extract served as capping agent
during the formation of AgCl-NPs. Creation of AgCl-NPs
was confirmed by UV–visible spectroscopy, powder X-ray
diffraction, energy-dispersive X-ray spectroscopy, and highresolution
transmission electron microscopy, while the morphology
and size analyses were characterized using highresolution
transmission electron microscopy and dynamic
light scattering. After optimization of some experimental conditions,
particularly pH, a simple and facile system was developed
for the naked-eye detection of bisphenol-A. Moreover, a
theoretical study of AgCl interaction with bisphenol-A was
performed at the density functional level of theory in both
gas and solvent phases. Theoretical results showed that electrostatic
and van der Waal interactions play important roles in
complexation of bisphenol-Awith AgCl-NPs, which can lead
to aggregation of the as-prepared AgCl-NPs and results in
color change from specific yellow to dark purple, where a
new aggregation band induced at 542 nm appears. The absorbance
at 542 nm was found to be linearly dependent on the
bisphenol-A concentration in the range of 1 × 10−6–1 ×
10−4 M, with limit of detection of 45 nM. In conclusion, obtained
results from the present study can open up an innovative
application of the green synthesis of AgCl-NPs using
brown algae extract as colorimetric sensor
Development of a new colorimetric assay for detection of bisphenol-A in aqueous media using green synthesized silver chloride nanoparticles: experimental and theoretical study.
Abstract
In the present study, a cost-effective, green and simple synthesis method was applied for preparation of stable silver chloride nanoparticles (AgCl-NPs). The method was done by forming AgCl-NPs from Ag+ ions using aqueous extract of brown algae (Sargassum boveanum) obtained from the Persian Gulf Sea. This extract served as capping agent during the formation of AgCl-NPs. Creation of AgCl-NPs was confirmed by UV-visible spectroscopy, powder X-ray diffraction, energy-dispersive X-ray spectroscopy, and high-resolution transmission electron microscopy, while the morphology and size analyses were characterized using high-resolution transmission electron microscopy and dynamic light scattering. After optimization of some experimental conditions, particularly pH, a simple and facile system was developed for the naked-eye detection of bisphenol-A. Moreover, a theoretical study of AgCl interaction with bisphenol-A was performed at the density functional level of theory in both gas and solvent phases. Theoretical results showed that electrostatic and van der Waal interactions play important roles in complexation of bisphenol-A with AgCl-NPs, which can lead to aggregation of the as-prepared AgCl-NPs and results in color change from specific yellow to dark purple, where a new aggregation band induced at 542 nm appears. The absorbance at 542 nm was found to be linearly dependent on the bisphenol-A concentration in the range of 1 × 10-6-1 × 10-4 M, with limit of detection of 45 nM. In conclusion, obtained results from the present study can open up an innovative application of the green synthesis of AgCl-NPs using brown algae extract as colorimetric sensors
Association of Pathogen Burden and Hypertension: The Persian Gulf Healthy Heart Study
background
Chronic infection with cytomegalovirus (CMV), Chlamydia pneumoniae,
herpes simplex virus 1 (HSV-1), and Helicobacter pylori may contribute
to essential hypertension. However, the evidence now available does
not clarify whether the aggregate number of pathogens (pathogen
burden) may be associated with hypertension.
methods
Sera from 1,754 men and women aged ≥25 years were analyzed for
immunoglobulin G antibodies to C. pneumoniae, HSV-1, H. pylori,
and CMV using enzyme-linked immunosorbent assay. The aggregate
number of seropositives to the studied viral and bacterial agents was
defined as pathogen burden. Hypertension was defined according to
World Health Organization criteria.
results
A total of 459 (26.3%) of the subjects had hypertension. In the hypertensive
group, 4.2% had 0 or 1 pathogens present, 20.6% had 2, 43.2%
had 3, and 32.1% had 4; in the normotensive group, 7.9% had 0 or 1,
28.4% had 2, 42.7% had 3, and 21.0% had 4. Of the 4 studied pathogens,
H. pylori seropositivity showed a significant independent association
with hypertension (odds ratio (OR) =1.37; 95% confidence interval
(CI) =1.05–1.79; P = 0.02). In multiple logistic regression analyses, the
pathogen burden did not show a significant independent association
with hypertension. Coinfection with H. pylori and C. pneumoniae
was significantly associated with hypertension compared with double
seronegativity after adjustment for age, sex, chronic low-grade inflammation,
and cardiovascular risk factors (OR = 1.68; 95% CI = 1.14–2.47;
P = 0.008].
conclusions
The pathogen burden was not associated with hypertension. However,
coinfection with C. pneumoniae and H. pylori showed a significant association
with essential hypertension, independent of cardiovascular risk
factors and chronic low-grade inflammation.
Keywords: blood pressure; Chlamydia pneumoniae; cytomegalovirus;
Helicobacter pylori; herpes simplex virus; hypertension; pathogen
Phospholipase A2 activity of the Persian Gulf upside-down jellyfish venom (Cassiopea andromeda)
Background: The venomous jellyfish Cassiopea andromeda can produce envenomation and different
toxicological and biological effects by their nematocysts. The phospholipase A2 enzymes (PLA2) are toxic
and induce various pharmacological effects including neurotoxicity, myotoxicity, and anticoagulant
activities. The main aim of the current project was to screen the in vitro PLA2 activity of the C. andromeda
crude venom. To better understand the experimental result; a molecular docking study was also performed.
Materials and methods: The live specimens were collected from Nayband lagoon, by a trawl net, and
separation of their tentacles was done according to Bloom 's et al., method. The PLA2 activity of crude
venom was performed according to the acidimetric method of Tan and Tan. The lyophilized venom was
subjected to Gas Chromatography/ Mass Spectroscopy, and the obtained structures were used for docking
study against PLA2. The indoxam was considered as standard control.
Results: The PLA2 activity of the jellyfish crude venom was 413 ±0.08 µmol/min/mg. Analysis of the crude
venom detected seven compounds (i-vii) using GC-MS. Docking data was also confirmed the experimental
results. According to the docking results, the highest affinity [-6.7 (kcal/mol)] was observed in the compound
“Pregn-5-ene-3,11-dione, 17,20:20,21 bis [methylenebis(oxy)]-, cyclic 3-(1,2-ethane diyl acetal”.
Conclusions: A high PLA2 level was found in the venom of C. andromeda. There was a good correlation
between in vitro and in silico studies
Omentin-1, visfatin and adiponectin levels in relation to bone mineral density in Iranian postmenopausal women
The potential link between infection with Chlamydia
pneumoniae or Helicobacter pylori and osteoporosis
has not been investigated in population-based longitudinal
studies. A total of 250 healthy postmenopausal women
who participated in a prospective cohort study were evaluated
for IgG antibodies directed against C. pneumoniae and
H. pylori, osteoprotegerin (OPG), the receptor activator of
nuclear factor kappa B ligand (RANKL), CrossLaps, and
osteocalcin. Bone mineral density (BMD) was measured at
the femoral neck and lumbar spine at baseline and at follow-
up 5.8 years later. There were no significant differences
in age-adjusted bone turnover markers, OPG, RANKL,
the RANKL/OPG ratio, and BMD between the C. pneumoniae
and H. pylori IgG seropositive and seronegative
subjects (P > 0.05). Neither C. pneumoniae nor H. pylori
IgG seropositivity was associated with age-and body massindex-adjusted BMD at the femoral neck and lumbar spine
or bone loss at the 5.8-year follow-up. In logistic regression
analysis, neither C. pneumoniae nor H. pylori IgG seropositivities
predicted incident lumbar or spine osteoporosis
5.8 years later. In conclusion, neither C. pneumoniae nor
H. pylori IgG seropositivity was associated with bone turnover
markers, the RANKL/OPG ratio, BMD, or bone loss
in postmenopausal women. In addition, chronic infection
with C. pneumoniae or H. pylori did not predict incident
osteoporosis among this group of women