MYB10 and MYB72 are Required for Growth under Iron-Limiting Conditions

Iron is essential for photosynthesis and is often a limiting nutrient for plant productivity. Plants respond to conditions of iron deficiency by increasing transcript abundance of key genes involved in iron homeostasis, but only a few regulators of these genes have been identified. Using genome-wide expression analysis, we searched for transcription factors that are induced within 24 hours after transferring plants to iron-deficient growth conditions. Out of nearly 100 transcription factors shown to be up-regulated, we identified MYB10 and MYB72 as the most highly induced transcription factors. Here, we show that MYB10 and MYB72 are functionally redundant and are required for plant survival in alkaline soil where iron availability is greatly restricted. myb10myb72 double mutants fail to induce transcript accumulation of the nicotianamine synthase gene NAS4. Both myb10myb72 mutants and nas4-1 mutants have reduced iron concentrations, chlorophyll levels, and shoot mass under iron-limiting conditions, indicating that these genes are essential for proper plant growth. The double myb10myb72 mutant also showed nickel and zinc sensitivity, similar to the nas4 mutant. Ectopic expression of NAS4 rescues myb10myb72 plants, suggesting that loss of NAS4 is the primary defect in these plants and emphasizes the importance of nicotianamine, an iron chelator, in iron homeostasis. Overall, our results provide evidence that MYB10 and MYB72 act early in the iron-deficiency regulatory cascade to drive gene expression of NAS4 and are essential for plant survival under iron deficiency

The Iowa Homemaker vol.22, no.5

Keeping Up With Today, Mary Lou Springer, page 2 Portraits in Sculpture, Virginia Carter, page 3 American Dyes Go to War, Du Pont Magazine, page 4 Vicky Celebrates A Warm Holiday, Virginia Brainard, page 6 Food Experts Back the Victory Effort, Dorothy Olson, page 8 Dehydration Gains in Scope, Mary Schmidt, page 9 What’s New In Home Economics, Helen Horton, page 10 His Christmas Box From You, Catherine Tidemanson, page 12 Across Alumnae Desks, Mary Ellen Sullivan, page 14 In an Ordnance Cafeteria, Dorothy Walker, page 16 Bookmarks, Eileen Dudgeon, page 17 That Hosiery Problem, Ann Mason, page 18 Alums in the News, Harriet Zook, page 2

Prospectus, March 13, 1985

https://spark.parkland.edu/prospectus_1985/1006/thumbnail.jp

Immortalized myogenic cells from congenital muscular dystrophy type1A patients recapitulate aberrant caspase activation in pathogenesis: a new tool for MDC1A research

BACKGROUND: Congenital muscular dystrophy Type 1A (MDC1A) is a severe, recessive disease of childhood onset that is caused by mutations in the LAMA2 gene encoding laminin-alpha2. Studies with both mouse models and primary cultures of human MDC1A myogenic cells suggest that aberrant activation of cell death is a significant contributor to pathogenesis in laminin-alpha2-deficiency. METHODS: To overcome the limited population doublings of primary cultures, we generated immortalized, clonal lines of human MDC1A myogenic cells via overexpression of both CDK4 and the telomerase catalytic component (human telomerase reverse transcriptase (hTERT)). RESULTS: The immortalized MDC1A myogenic cells proliferated indefinitely when cultured at low density in high serum growth medium, but retained the capacity to form multinucleate myotubes and express muscle-specific proteins when switched to low serum medium. When cultured in the absence of laminin, myotubes formed from immortalized MDC1A myoblasts, but not those formed from immortalized healthy or disease control human myoblasts, showed significantly increased activation of caspase-3. This pattern of aberrant caspase-3 activation in the immortalized cultures was similar to that found previously in primary MDC1A cultures and laminin-alpha2-deficient mice. CONCLUSIONS: Immortalized MDC1A myogenic cells provide a new resource for studies of pathogenetic mechanisms and for screening possible therapeutic approaches in laminin-alpha2-deficiency

The Iowa Homemaker vol.23, no.2

Presenting 1943 Veishea, Dorothy Walker, page 1 Keeping Up With Today, Margaret Ralston, page 4 Letter from a SPAR, Mary Leffler, page 5 Demand for Day Nurseries, Marjory Gillingham, page 6 Freezing Preserves Victory Foods, Frances Madigan, page 7 Gardens Challenge Faculty, Lois Stewart, page 8 Food Affects Morale, Dr. Lowell Selling, page 9 Vicky Favors Simplicity, Mary Lou Springer, page 10 An Economist Interprets Food Problems, Dorothy Conquest, page 11 Grooming for a Career, Mary Schmidt, page 12 Students Enlist, Annette DeLay, page 13 The Red Cross Canteen Corps Mobilize, page 14 What’s New in Home Economics, Lily Houseman, page 16 Women to Know, Josephine Ahern, page 18 Home Economists Plan for the WAAC, Frances Kerekes, page 19 Canada Organizes for Nutrition, Mary Ellen Sullivan, page 20 Experience Gleaned, Janet Russell, page 22 Eggs Spell Good Nutrition, Marian Loofe, page 23 Commissioned in Dietetics, Victoria McKibben, page 24 Across Alumnae Desks, Virginia Carter, page 26 Whole Grains Fortify, Shirley Like, page 28 Designed for Art Majors, JoAnne Nicholson, page 29 Alums in the News, Rachel Ann Lusher, page 31 Meals on the Move, Catherine Tidemanson, page 3

Prospectus, April 4, 1984

PARKLAND STUDENT RECEIVES STATEWIDE AWARD; News Digest; Parkland welcomes visitors; Problems? Let us help--NEW--Advice Column; \u27Images\u27 \u2784 starts production; PC Happenings: Kidney stone treatment is seminar topic, May fest exhbitors to register, Parkland hosting hairstyling, Martial arts demonstration, March of Dimes walkamerica, Stu-Go to survey; Two Parkland students to go to conference; Blood drive; Board considers closing U.H.S.; Did You Know...; Creative Corner...Especially for you!!: Buzz Galaxy, Father, Dear Father, Unicorns, My Friends and Me, A low rumble starts...; Archaeological Field School.....Experiencing the world ......... the way it really is: Shawnee National Forest: Extreme weather conditions; \u27Racing\u27--a good traditional movie; Rebel with a cause; Disney to release \u27Touchstone Films\u27; Classified; International Cafe during Open House; Parkland Student returns home; Parkland fall registration; What to do with a home computer; Have a question about the new phone systems?; Spring brings on large scale housecleaning; Women\u27s hats coming back; Elmhurst defeats Peoria; Cobra men split two; We goofed: Oops...; Quincy wins its second straight championship; Expos to take N.L. East; Fitness Center to open; Softball newshttps://spark.parkland.edu/prospectus_1984/1026/thumbnail.jp

Control of disseminated intravascular coagulation in Klippel-Trenaunay-Weber syndrome using enoxaparin and recombinant activated factor VIIa: a case report

<p>Abstract</p> <p>Introduction</p> <p>Vascular malformation is associated with coagulopathies, especially when hemostasis is challenged.</p> <p>Case presentation</p> <p>We present the case of an 11-year-old Hispanic girl with Klippel-Trenaunay-Weber syndrome that developed disseminated intravascular coagulation after minor surgery, which was controlled by blood product transfusions and enoxaparin to address an ongoing consumptive coagulopathy. The patient, however, developed bacteremia and liver trauma that resulted in severe bleeding. To the best of our knowledge, we report here the first known instance of administering recombinant coagulation factor VIIa to control acute bleeding in a patient with Klippel-Trenaunay-Weber syndrome.</p> <p>Conclusions</p> <p>This case illustrates the concept of enoxaparin maintenance to suppress an ongoing consumptive coagulopathy and the use of recombinant coagulation factor VIIa to control its potentially fatal severe bleeding episodes.</p

Prospectus, March 7, 1984

CONGRESS RETURNS TO A \u27MOST IMPORTANT\u27 STUDENT AID DEBATE; News Digest; Four paintings stolen from Parkland; \u27Job Training and Partnership Act\u27: JTPA \u27good opportunity for dedicated\u27; PC Happenings: TV programs for busy parents, Parkland announces registration, Polish folk arts at Parkland; Sargent talks to Lifelong Learners\u27 Club; Yaxley scholarship; Stu-Go News; \u27Planned Parenthood\u27--pro choice organization; In the Library--Paperbacks; Nolen enjoys differences; What is Sadie Hawkins Day?; Did You Know...; Scholarship news for real estate students; Tuexdo styles are changing; What did you thinnk of the Grammy Awards?; Classifieds; \u27Good looking coed\u27 hunt draws lots of photos; Urbana construction will increase; Learning to relax can kill stress; \u27Women\u27s History Week\u27 honored; Parkland Christian Fellowship to sponsor conference; Weather conditions force closing; Math contest at Parkland; Financia; aid for summer and fall; The latest in swimwear; \u27Western look\u27 changing; Creative Corner...Especially for you!!: Believe..., Creed, Dream, The End, Darkness...; All Up to Me, Listening to an old..., Father to Son, How to know just what is real..., For Larry, Hi, I\u27m crazy ...; Bite the Bullet, Answer, Why can\u27t we see?, Love sparked nu the heat of summer...,Keg, A low rumble starts..., Oh, Mothers..., Has the human race forgot to look at a sunset..., I tried to play your game..., You\u27ve let me down...; Jackson leads the way at this year\u27s Grammy awards; Dexter--valuable asset; $50,000 and silence \u27cheap way for MSU to avoid lawsuit\u27; In the Library--; Channel 12 fund drive stresses \u27TV worth paying for\u27; Woody Allen\u27s latest hilarious; \u27Foghat\u27 hits C/U March 18; Predictions for the upcoming Academy Awards; \u27Big Country\u27 to appear; \u27Full Metal Jacket\u27 talent search; \u27The Right Stuff\u27 finally opens in area; Energetic movie should do well; Baseball Schedule; Bank president--NFL referee; High School Notes; Softball Schedule; Cobra baseball to start; Women win tournament; Lake Land advances in sectional; Mens basketball scores; I.M. News; Cobra men advance to sectional; Cobras end up with last year\u27s record; Tae Kwon Do showed display; Cooper remembers 6 good years; First basketball coach tells how it was; NJCAA meethttps://spark.parkland.edu/prospectus_1984/1029/thumbnail.jp

Prospectus, April 18, 1984

CHORAL UNION DEMANDING AND REWARDING; News Digest; Is your child part of the identi-child program?; Budding playwrights get chance to have work produced; PC Happenings: Parkland wins Automotive Contest, German Club annual Springfest, EMT workshop offered, Petitions available for Stu-Go, Stu-Go lacks quorum; Here Comes the Easter Bunny (or the Easter Hare?); Faces We Make; Former Prospectus entertainment writer: Alender services held; Strange things at the library; Only Food Service Management course in area: Sutton leads Food Service Management program; Criminal justice program well rounded; Running may increase protein need; Easter means Easter egg time; Second Wind hosts race; Did You Know...; Classifieds; Creative Corner...Especially for you!!: Recollections, She\u27s More than a Nurse, You are a corn-fed steak..., Memories..., A fire burns, AKTING??????????????..., Sometimes in their heed to tact..., Clouds, Disturb Not the Dead, Follow the Wind, I don\u27t know why I bother..., Innoncent Eyes, Please tell me what it is you want..., Running scared from yourself and tripped, babe..., Time Bomb, Don\u27t go away--Linger on..., Class, Raggedy Ann lay in the back of an old station wagon..., Too Late, To God; Ice Capades comes to town: Skaters say it\u27s hard work but well worth it; few surprises during Hollywood\u27s biggest night; \u27Queen\u27 film at Assembly Hall; Director shines in film; \u27Go-Go\u27s\u27 are back on track; \u27Weird Al\u27 hits big time; Fan Club to host film; Parkland College 1984 baseball roster; Cobra men lose two; 1984 Parkland Outdoor Track Bests; Sports Digest; Women lose twin bill; Prospectus survey resultshttps://spark.parkland.edu/prospectus_1984/1024/thumbnail.jp