Goat milk free fatty acid characterization during conventional and ohmic heating pasteurization

The disruption of the milk fat globule membrane can lead to an excessive accumulation of free fatty acids in milk, which is frequently associated with the appearance of rancid flavors. Solid-phase microextraction and gas chromatography techniques have been shown to be useful tools in the quantification of individual free fatty acids in dairy products providing enough sensitivity to detect levels of rancidity in milk. Therefore, the aim of this study was to characterize the short-chain and medium-chain free fatty acid profile in i) raw untreated goat milk; ii) raw goat milk passing through pumps and heating units (plate-and-frame heat exchanger and ohmic heater); and iii) processed goat milk by conventional and ohmic pasteurization to determine the influence of each treatment in the final quality of the milk. Multivariate statistical analysis has shown that the treatments studied were not responsible for the variability found on free fatty acid contents. In particular, it was possible to conclude that ohmic pasteurization at 72°C for 15 s did not promote an extended modification of free fatty acid contents in goat milk when compared with that of conventional pasteurization. Furthermore, principal component analysis showed that the capric acid can be used to discriminate goat’s milk with different free fatty acid concentrations. Hierarchical cluster analysis showed evidence of the existence of correlations between contents of short and medium chain free fatty acids in goat milk

In-season internal and external training load quantification of an elite European soccer team

Elite soccer teams that participate in European competitions need to have players in the best physical and psychological status possible to play matches. As a consequence of congestive schedule, controlling the training load (TL) and thus the level of effort and fatigue of players to reach higher performances during the matches is therefore critical. Therefore, the aim of the current study was to provide the first report of seasonal internal and external training load that included Hooper Index (HI) scores in elite soccer players during an in-season period. Nineteen elite soccer players were sampled, using global position system to collect total distance, high-speed distance (HSD) and average speed (AvS). It was also collected session rating of perceived exertion (s-RPE) and HI scores during the daily training sessions throughout the 2015-2016 in-season period. Data were analysed across ten mesocycles (M: 1 to 10) and collected according to the number of days prior to a one-match week. Total daily distance covered was higher at the start (M1 and M3) compared to the final mesocycle (M10) of the season. M1 (5589m) reached a greater distance than M5 (4473m) (ES = 9.33 [12.70, 5.95]) and M10 (4545m) (ES = 9.84 [13.39, 6.29]). M3 (5691m) reached a greater distance than M5 (ES = 9.07 [12.36, 5.78]), M7 (ES = 6.13 [8.48, 3.79]) and M10 (ES = 9.37 [12.76, 5.98]). High-speed running distance was greater in M1 (227m), than M5 (92m) (ES = 27.95 [37.68, 18.22]) and M10 (138m) (ES = 8.46 [11.55, 5.37]). Interestingly, the s-RPE response was higher in M1 (331au) in comparison to the last mesocycle (M10, 239au). HI showed minor variations across mesocycles and in days prior to the match. Every day prior to a match, all internal and external TL variables expressed significant lower values to other days prior to a match (p<0.01). In general, there were no differences between player positions. Conclusions: Our results reveal that despite the existence of some significant differences between mesocycles, there were minor changes across the in-season period for the internal and external TL variables used. Furthermore, it was observed that MD-1 presented a reduction of external TL (regardless of mesocycle) while internal TL variables did not have the same record during in-season match-day-minus.: The authors state that there were no salaries’ fund from a tobacco company. Also, the authors are not aware of any competing interests. This project was supported by the National Funds through FCT—Portuguese Foundation for Science and Technology (UID/DTP/04045/2013)—and the European Fund for Regional Development (FEDER) allocated by European Union through the COMPETE 2020 Programme (POCI-01-0145- FEDER-006969)—competitiveness and internationalization (POCI). All funding received for this work from any of the following organizations: National Institutes of Health (NIH); Welcome Trust; Howard Hughes Medical Institute (HHMI). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.info:eu-repo/semantics/publishedVersio

In-season training load quantification of one-, two- and three-game week schedules in a top European professional soccer team

Top European soccer teams that play in UEFA competitions often participate in one, two- or three-games per week. Therefore, it is necessary to ensure optimal match-day performance and full recovery. The aim of this study was to quantify internal and external training load (TL) within five microcycles: M1 and M2 - one-game weeks; M3 and M4 - two-game weeks; M5 - three-game week). Thirteen elite soccer players participated in this study. A global positioning system (GPS) was used to measure the total distance covered and distances of different exercise training zones (1-5), the session ratings of perceived exertion (s-RPE) scores and the amount of creatine kinase (CK) created during daily training sessions for the 2015-2016 in-season period. The data were analysed with respect to the number of days prior to a given match. The main results indicate that there was a significant difference in training intensity for zone 1 between M2 and M4 (4010.2 ± 103.5 and 4507.6 ± 133.0 m, respectively); a significant difference in training intensity for zone 3 between M1 and M5 (686.1 ± 42.8 and 801.2 ± 61.2 m, respectively); a significant difference in the duration of the training sessions and matches between M2 and M5 (69.2 ± 2.1 and 79.6 ± 2.3) and M3 and M5 (69.7 ± 1.0 and 79.6 ± 2.3); and finally, there was a significant difference in CK between M3 and M2 (325.5 ± 155.0 and 194.4 ± 48.9). Moreover, there was a significant decrease in TL in the last day prior to a match, for all microcycles and all variables. There was no significant difference with respect to s-RPE. This study provides the first report of daily external and internal TLs and weekly accumulated load (training sessions and match demands) during one, two, and three-game week schedules in a group of elite soccer players. Expected significant differences are found in daily and accumulated loads for within- and between-game schedules. A similar pattern is exhibited for one- and two-game week microcycles regarding the day before the match, which exhibits a decrease in all variables. Despite the different number of games played per week, TL remain similar between microcycles for zone 2 and 5, plus s-RPE.This project was supported by the National Funds through FCT—Portuguese Foundation for Science and Technology (UID/DTP/04045/2013) and the European Fund for Regional Development (FEDER) allocated by European Union through the COMPETE 2020 Programme (POCI-01-0145-FEDER-006969)—competitiveness and internationalization (POCI). The authors disclose funding received for this work from any of the following organizations: National Institutes of Health (NIH); Welcome Trust; Howard Hughes Medical Institute (HHMI); and other(s).info:eu-repo/semantics/publishedVersio

Fostering computational thinking skills : a didactic proposal for elementary school grades

There is a growing presence of technology in the daily lives of elementary school students, with a recent exponential rise due to the constraints of remote teaching during the COVID-19 pandemic. It is important to understand how the education system can contribute to helping students develop the required skills for technological careers, without neglecting its obligation to create conditions that allow them to acquire transversal skills and to enable them to exercise full citizenship. The integration of Educational Robotics and block programming activities in collaborative learning environments promotes the development of computational thinking and other ICT skills, as well as critical thinking, social skills, and problem solving. This paper presents a theoretical proposal of a didactic sequence for the introduction to educational robotics and programming with Scratch Jr. It is composed of three learning scenarios, designed for elementary school teaching. Its main goal is to create conditions that favour the development of computational thinking in a collaborative learning environment. With increasing complexity and degree of difficulty, all the tasks root from a common problem: How can we create an algorithm that programs the robot/sprite to reach a predetermined position?info:eu-repo/semantics/publishedVersio

Using educational robotics in pre-service teacher training : orchestration between an exploration guide and teacher role

The proper integration of technology in teaching and learning processes must consider the role of teachers and students, as well as the design of tasks and the context in which they are implemented. Teachers’ perceived self-efficacy significantly influences their willingness to integrate educational robotics (ER) into their practice, so initial teacher training should provide opportunities for teachers to participate in structured activities that integrate ER. In this study, a class of pre-service teachers from an initial teacher training programme were provided with their first contact with an ER platform through the use of a simulator. We present the design process of a student exploration guide and teacher guide, developed over three iterative cycles of implementation, assessment and redesign. The analysis of the data collected allowed for improvements in the design of the tasks, the graphic component of the student exploration guide, and more precise indications for the teacher’s actions. The main contribution of this study is the chain orchestration between the simulator, student exploration guide and teacher guide, which allowed pre-service teachers to solve a set of challenges of increasing complexity, thereby progressively decreasing their difficulties and contributing to an adequate integration of ER in their future teaching practices.info:eu-repo/semantics/publishedVersio

Use of fluent for the development of a di-si engine

The recent surge of electric vehicles has put pressure on the development and manufacture of batteries. However, batteries are still expensive, bulky and heavy, creating the need for inboard electricity generation using an internal combustion engine, usually referred as “range extender”. This paper presents the initial development of a DI-SI engine to work as range extender, focusing on the interaction between fuel spray and airflow inside the combustion chamber. To enable efficient combustion of lean and extra lean mixtures, a technique called stratified charge, is used. With direct injection spark ignition (DI-SI) engines it is important, under part load, to direct the fuel spray to the vicinities of the spark plug, enabling a fast and stable combustion of a lean mixture. A rich mixture region is created near the spark plug allowing an easy kernel formation and development. There are three types of systems for “directing” the fuel spray towards the spark plug: wall guided, air guided and spray guided. The developed design is a mixture of wall and air guided systems and the idea is to inject the spray towards the piston crown and to divert it to the spark plug location by the barrel swirl existent within the combustion chamber at this time. The system development was carried out using CFD FLUENT code. The study comprises three parts, the design of the components and its location (combustion chamber, piston crown, intake passage and injector location and aim), the air flow modeling and finally, the two phase modelling. A simple engine geometry and mesh were created in the Ansys CFD software. The air flow was considered to be transient, incompressible, Newtonian and viscous turbulent. The turbulence model used was the standard k-ε model, since it is the most common, simple and well-known model of turbulence. The spray has been simulated using the Discrete Phase Model. The Lagrangian discrete phase model in Fluent™ follows the Euler-Lagrange approach, where the fluid phase is treated as a continuum by solving the time-averaged Navier-Stokes equations, while the dispersed phase is solved by tracking a large number of particles through the calculated flow field. Preliminary results are now being obtained.MIT Portugal, Fundação para a Ciência e a Tecnologia (FCT

Long-term management practices successfully reduce bird-related electrical faults in a transmission grid increasingly used by white storks for nesting

Bird nests on transmission lines can cause electrical faults which reduce service reliability. To address this problem, since the mid-90s, the Portuguese Transmission System Operator (TSO) has undertaken management actions to discourage white storks Ciconia ciconia from nesting in hazardous locations of the pylons. Here, we compiled and analyzed an 18-year series of data on electrical faults, TSO management actions to tackle these, and stork nests on transmission pylons in Portugal to: (a) determine the relative importance of bird-related faults over the total number of faults; (b) describe variations in bird-related faults across time (season of the year and time of the day); (c) describe spatial variations in bird-related faults and their association with the occurrence of white stork nests on pylons; and (d) analyze the trends, over the years, of the number of white stork nests on pylons, the TSO management actions and their effectiveness in reducing bird-related fault rates. Overall, birds accounted, on average, for 25.3% of the electrical faults in the transmission network, with the vast majority being attributed to white storks. The seasonal pattern of bird-related faults showed higher rates in April and in October–November. Faults occurred more often during the night period, when storks spend more time on the pylons. We found a positive spatial relationship between the electrical fault rate and the proportion of pylons with stork nests (and the correlated number of nests per 100 km of line). There was, however, considerable variation in the fault rates not explained by the stork nest variables, particularly during the non-breeding season. The TSO management actions (namely removal/translocation of nests in hazardous locations of the pylons, installation of anti-perching devices and provision of alternative nesting platforms) significantly reduced, as a whole, the annual number of bird-related faults between 2001 and 2018, despite the three-fold increase in the number of white stork nests on transmission pylons. A deeper understanding of how white storks use the transmission pylons outside the breeding season is needed, so that targeted management actions can be taken to reduce the remaining bird-related fault rates to residual levels.info:eu-repo/semantics/publishedVersio

Response of vertebrate scavengers to power line and road rights-of-way and its implications for bird fatality estimates

Linear infrastructures, such as power lines and roads, are an important source of bird mortality. However, little is known on the potential effect of these infrastructures on local scavenger guilds, their foraging activity and the resulting bird carcass removal patterns. This is an important source of bias in studies aiming to quantify bird fatalities due to linear infrastructures. We used camera-traps to record scavenger identity and persistence patterns of bird carcasses placed close to linear infrastructure and nearby controls in two Mediterranean agricultural regions. We found that linear infrastructure influence on scavenger identity varied depending on the region. Contrary to expectations, linear infrastructure presence had either none or a positive effect on carcass persistence, meaning that carcasses placed within power line or road rights-of-way were not removed faster than the ones placed in controls. We conclude that linear infrastructure effect on vertebrate scavenging patterns is likely to be region-specific, and that reliable correction factors for carcass removal-bias in bird fatality estimates require site-specific experiments to characterize local scavenging processesinfo:eu-repo/semantics/publishedVersio

Monitoring goat milk quality during pasteurisation and ohmic treatment using UV-VIS-SWNIR spectroscopy

This study evaluates the effectiveness of UV-VIS-SWNIR spectroscopy of goat milk quality degradation during pasteurisation and ohmic heating, being performed on: i) raw goat milk; ii) non-processed milk but passing though pumps, pasteurizer and ohmic heater; and iii) processed milk by pasteurisation and ohmic heating. Spectra were collected by a transmittance probe for UV-VIS and UV-NIR wavelengths. The samples temperature was recorded (18.0 ± 2.0oC) and the probe was always checked for bubble formation or fat residues on lens/mirror system. The integration time was set to 25s and 4s for the collection of UV-VIS and VIS-NIR spectra respectively. Data analysis was performed on each product and for each spectral range independently. The spectra were normalized by its maximum intensity and the corrected for using a robust multiplicative scatter correction algorithm. A principal component analysis was performed to the pre-processed spectra. Results show that UV-VIS-SWNIR reflectance spectroscopy provides a quick and fast assessment of goat milk characteristics and thus it can be used as an indication of the overall product variability, allowing to develop monitoring and control models for both pasteurisation and ohmic heating of goat milk.Fundação para a Ciência e a Tecnologia (FCT)EDERPRIME progra