Response to oxidative stress induced by cadmium and copper in tobacco plants (Nicotiana tabacum) engineered with the trehalose-6-phosphate synthase gene (AtTPS1)

The response of tobacco plants genetically engineered with the AtTPS1 gene to stress induced by excess Cu and Cd was evaluated in hydroponic solution (100 and 400 lM Cu and 50 and 200 lM Cd) after a 48 h exposure. Two transgenic lines, transformed with the AtTPS1 (trehalose-6-phosphate synthase) gene from Arabidopsis, with different levels of trehalose-6-phosphate synthase expression (B5H, higher and B1F, lower), and a wild type (WT) were investigated. Protein content, antioxidative enzymes (CAT, POD, SOD, and APX), glucose, fructose, lipid peroxidation, hydrogen peroxide and Cd and Cu contents were determined in leaves. The two transgenic lines were differently influenced by Cd and Cu exposure as they induced a different antioxidant enzymatic defense response. B1F and B5H plants showed a better acclimation to Cd and excess Cu compared to WT. Furthermore B1F was more tolerant than B5H to Cd and excess Cu. B1F accumulated less Cd and Cu in leaves, probably due to a more efficient exclusion mechanism. Catalase was shown to be the most important enzyme in the antioxidative system of these plants

Da complexidade do conceito de tempo geológico às dificuldades de alunos do 3.º ciclo do ensino básico

Esta comunicação insere-se num projecto de investigação educacional em curso, que envolve professores dos Ensinos Básico e Secundário, investigadores em Didáctica das Ciências e especialistas em Geologia. Com ele, pretende-se reflectir sobre o conceito de tempo e aprofundar a compreensão das dificuldades sentidas por alunos do 3º Ciclo do Ensino Básico relativamente ao tempo geológico para, em conformidade, conceber, desenvolver e avaliar estratégias curriculares que contribuam para a melhoria da literacia científica dos discentes

Age-friendly cities - some exploration data on Portalegre County

Para a OMS “O envelhecimento ativo é o processo de otimização de condições de saúde, participação e segurança, de modo a melhorar a qualidade de vida à medida que as pessoas envelhecem” dependendo de vários fatores, influências e sentimentos (OMS, 2007 citado em FCG, 2009: 5). Assim é essencial conhecermos as condições de vida destas pessoas, contemplando dimensões ligadas à territorialidade ao nível dos espaços lúdico-desportivos, habitacionais, de transporte, serviço comunitário e saúde. Importa perceber ainda a forma como é efetuada a participação e inclusão social, a participação cívica bem como as potencialidades e constrangimentos da comunicação e informação que é disponibilizada ao idoso, tendo como objetivo último melhorar as condições oferecidas aos idosos. Foi objetivo deste trabalho, gerar um retrato local onde as pessoas idosas e instituições possam fazer a sua autoavaliação e registar os progressos efetuados baseado em perceções. Como estratégia metodológica, utilizámos o questionário elaborado pela OMS e cedido em Portugal pela Associação Vida

Metamitron and Shade Effects on Leaf Physiology and Thinning Efficacy of Malus × domestica Borkh

Thinning strategies, namely shade or photosynthetic inhibitors, rely on the reduction of carbon supply to the fruit below the demand, causing fruit abscission. In order to clarify the subject, seven field trials were carried out in Lleida, Girona, and Sint-Truiden (2017 + 2018), using orchards of ‘Golden’ and ‘Gala’ apple trees. At the stage of 9–14-mm fruit diameter, four treatments were implemented: (A) CTR-control, trees under natural environmental conditions; (B) SN-shaded trees, trees above which shading nets reducing 50% of irradiance were installed 24 h after metamitron application date—without application of metamitron—and removed after five days; (C) MET-trees sprayed with 247.5 ppm of metamitron; (D) MET + SN-trees submitted to the combined exposure to metamitron application and shading nets. Low radiation significantly increased metamitron absorption (36–53% in the three locations in 2018) and reduced its degradation. Net photosynthesis and stomatal conductance were strongly reduced in all treatments, with minimum values 2 days after spraying (DAS) and incomplete recovery 10 DAS in MET + SN. All treatments resulted in leaf sucrose and sorbitol decreases, leading to a negative carbon balance. SN and MET + SN promoted the highest thinning efficacy, increasing fruit weight and size, with MET + SN causing over-thinning in some trials. Leaf antioxidant enzymes showed moderate changes in activity increases under MET or MET + SN, accompanied by a rise of glutathione content and a reduction in ascorbate, however without lipid peroxidation. This work shows that environmental conditions, such as cloudy days, must be carefully considered upon metamitron application, since the low irradiance enhances metamitron efficacy and may cause over-thinninginfo:eu-repo/semantics/publishedVersio

COOPEDU IV — Cooperação e Educação de Qualidade

O quarto Congresso Internacional de Cooperação e Educação-IV COOPEDU, organizado pelo Centro de Estudos Internacionais (CEI) do Instituto Universitário de Lisboa e pela Escola Superior de Educação e Ciências Sociais do Instituto Politécnico de Leiria decorreu nos dias 8 e 9 de novembro de 2018, subordinado à temática Cooperação e Educação de Qualidade. Este congresso insere-se numa linha de continuidade de intervenção por parte das duas instituições organizadoras e dos elementos coordenadores e este ano beneficiou do financiamento do Instituto Camões, obtido através de um procedimento concursal, que nos permitiu contar com a participação presencial de elementos dos Países Africanos de Língua Portuguesa, fortemente implicados nas problemáticas da Educação e da Formação. Contou também com a participação do Instituto Camões e da Fundação Calouste Gulbenkian, entidades que sistematizaram a sua intervenção nos domínios da cooperação na área da educação nos últimos anos. A opção pela temática da qualidade pareceu aos organizadores pertinente e actual. Com efeito os sistemas educativos dos países que constituem a Comunidade de países de língua portuguesa têm implementado várias reformas mas em vários domínios mantem-se a insatisfação de responsáveis políticos, pedagogos, técnicos sociais face aos resultados obtidos. Aliás o caminho de procura da Qualidade é interminável porque vai a par da aposta na exigência e na promoção da cidadania e responsabilidade social. As comunicações que agora se publicam estão organizadas em dois eixos: o das Políticas da Educação e Formação e o das dimensões em que se traduzem essas políticas. Neste último eixo encontramos fios condutores para agregarmos as comunicações apresentadas

Antioxidative response of plants to oxidative stress induced by cadmium

This book edited by Dr. Mirza Hasanuzzaman and Dr. Masayuki Fujita presents a collection of 16 chapters written by 67 experts from 19 countries working on cadmium toxicity. This volume provides the readers with a background for understanding cadmium toxicity, its environmental and health aspects, and its remediation mechanisms. Various chapters included in this book provide a state-of-the-art account of the information as a resourceful guide suited for scholars and researchers working in the field of cadmium. This book is a invaluable resource for plant biologists, agriculturists, toxicologists, biochemists, environmental scientists, physiologists, pharmacologists, geneticists, molecular biologists; as well as graduate students in these discipline

Triagem interventiva: a caracterização de uma demanda

This paper analyzes the process of sorting through the intervening demand survey that reaches the Center for Research and Applied Psychology (CPPA) “Dr. Betti Katzenstein” UNESP Assis. The objective was to better understand the reality of conflicts that people face and, therefore, to characterize the patients who seek help in CPPA. With greater understanding of demand, it is possible to trace more consistent referrals and tailor the service to the school clinic profile of clients that demand. Recalling that the actual attendance of screening is already a form of interventional care, because it provides patient care from first contact. This was a documentary research, which had collected their data sheets of the CPPA trials conducted in 2011. 394 screenings were performed. We present the distribution of the demand for sex, age and complaint that motivated the search for care. It can be concluded that the characterization allows a customer routing more efficient services offered at the institution, as it contributes to a better understanding delineated in each case and a reduced number of dropouts in the screening process.Este trabalho faz uma análise do processo de triagem interventiva por meio do levantamento da demanda que chega ao Centro de Pesquisa e Psicologia Aplicada (CPPA) “Dra. Betti Katzenstein” da UNESP de Assis. O objetivo foi compreender melhor a realidade dos conflitos que essa população enfrenta e, com isso, caracterizar a clientela que busca auxílio no CPPA. Com uma maior compreensão da demanda, é possível traçar encaminhamentos mais condizentes e adequar o serviço da clínica-escola ao perfil da clientela que a procura. Lembrando que o próprio atendimento de triagem já é uma forma interventiva de atendimento, pois ele proporciona o cuidado do paciente desde o primeiro contato. Essa foi uma pesquisa documental, que teve seus dados coletados das fichas de triagens do CPPA realizadas em 2011. Foram feitas 394 triagens. É apresentada a distribuição da demanda por sexo, faixa etária e queixa que motivou a busca pelo atendimento. Pode-se concluir que a caracterização da clientela possibilita um direcionamento mais eficaz dos serviços oferecidos na instituição, visto que contribui para uma compreensão melhor delineada de cada caso e um número reduzido de desistências no processo de triagem

Metamitron and Shade Effects on Leaf Physiology and Thinning Efficacy of Malus × domestica Borkh

Thinning strategies, namely shade or photosynthetic inhibitors, rely on the reduction of carbon supply to the fruit below the demand, causing fruit abscission. In order to clarify the subject, seven field trials were carried out in Lleida, Girona, and Sint-Truiden (2017 + 2018), using orchards of ‘Golden’ and ‘Gala’ apple trees. At the stage of 9–14-mm fruit diameter, four treatments were implemented: (A) CTR-control, trees under natural environmental conditions; (B) SN-shaded trees, trees above which shading nets reducing 50% of irradiance were installed 24 h after metamitron application date—without application of metamitron—and removed after five days; (C) MET-trees sprayed with 247.5 ppm of metamitron; (D) MET + SN-trees submitted to the combined exposure to metamitron application and shading nets. Low radiation significantly increased metamitron absorption (36–53% in the three locations in 2018) and reduced its degradation. Net photosynthesis and stomatal conductance were strongly reduced in all treatments, with minimum values 2 days after spraying (DAS) and incomplete recovery 10 DAS in MET + SN. All treatments resulted in leaf sucrose and sorbitol decreases, leading to a negative carbon balance. SN and MET + SN promoted the highest thinning efficacy, increasing fruit weight and size, with MET + SN causing over-thinning in some trials. Leaf antioxidant enzymes showed moderate changes in activity increases under MET or MET + SN, accompanied by a rise of glutathione content and a reduction in ascorbate, however without lipid peroxidation. This work shows that environmental conditions, such as cloudy days, must be carefully considered upon metamitron application, since the low irradiance enhances metamitron efficacy and may cause over-thinning

Metamitron and shade effects on leaf physiology and thinning efficacy of Malus X domestica Borkh

Thinning strategies, namely shade or photosynthetic inhibitors, rely on the reduction of carbon supply to the fruit below the demand, causing fruit abscission. In order to clarify the subject, seven field trials were carried out in Lleida, Girona, and Sint-Truiden (2017 + 2018), using orchards of ‘Golden’ and ‘Gala’ apple trees. At the stage of 9–14-mm fruit diameter, four treatments were implemented: (A) CTR-control, trees under natural environmental conditions; (B) SN-shaded trees, trees above which shading nets reducing 50% of irradiance were installed 24 h after metamitron application date—without application of metamitron—and removed after five days; (C) MET-trees sprayed with 247.5 ppm of metamitron; (D) MET + SN-trees submitted to the combined exposure to metamitron application and shading nets. Low radiation significantly increased metamitron absorption (36–53% in the three locations in 2018) and reduced its degradation. Net photosynthesis and stomatal conductance were strongly reduced in all treatments, with minimum values 2 days after spraying (DAS) and incomplete recovery 10 DAS in MET + SN. All treatments resulted in leaf sucrose and sorbitol decreases, leading to a negative carbon balance. SN and MET + SN promoted the highest thinning e cacy, increasing fruit weight and size, with MET + SN causing over-thinning in some trials. Leaf antioxidant enzymes showed moderate changes in activity increases under MET or MET + SN, accompanied by a rise of glutathione content and a reduction in ascorbate, however without lipid peroxidation. This work shows that environmental conditions, such as cloudy days, must be carefully considered upon metamitron application, since the low irradiance enhances metamitron e cacy and may cause over-thinninginfo:eu-repo/semantics/publishedVersio